Dot-blot hybridization and RT-PCR detection of extra small virus (XSV) associated with white tail disease of prawn Macrobrachium rosenbergii
The availability of specific and reliable detection methods is essential for monitoring the health status of farmed species, particularly for viral diseases. Extra small virus (XSV), a virus-like particle, is associated with Macrobrachium rosenbergii Noda virus (MrNV) in white tail disease (WTD) of...
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| Veröffentlicht in: | Diseases of aquatic organisms 2004-01, Vol.58 (1), p.83-87 |
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| container_title | Diseases of aquatic organisms |
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| creator | SRI WIDADA, Joannes RICHARD, Véronique ZHENGLI SHI DONG QIAN BONAMI, Jean-Robert |
| description | The availability of specific and reliable detection methods is essential for monitoring the health status of farmed species, particularly for viral diseases. Extra small virus (XSV), a virus-like particle, is associated with Macrobrachium rosenbergii Noda virus (MrNV) in white tail disease (WTD) of M. rosenbergii. We developed 2 genome-based detection methods for the identification of XSV, namely dot-blot hybridization and a single-step RT-PCR. Detection limits were established and are ca. 2.5 pg and 5 fg of viral RNA for dot-blot hybridization and RT-PCR, respectively. Application of the methods to field samples indicated that some animals positively diagnosed with MrNV did not contain XSV, at least within the detection limit of the methodology. This raises the question of the actual role of XSV and its interactions with MrNV in WTD of M. rosenbergii. |
| doi_str_mv | 10.3354/dao058083 |
| format | Article |
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Extra small virus (XSV), a virus-like particle, is associated with Macrobrachium rosenbergii Noda virus (MrNV) in white tail disease (WTD) of M. rosenbergii. We developed 2 genome-based detection methods for the identification of XSV, namely dot-blot hybridization and a single-step RT-PCR. Detection limits were established and are ca. 2.5 pg and 5 fg of viral RNA for dot-blot hybridization and RT-PCR, respectively. Application of the methods to field samples indicated that some animals positively diagnosed with MrNV did not contain XSV, at least within the detection limit of the methodology. 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Extra small virus (XSV), a virus-like particle, is associated with Macrobrachium rosenbergii Noda virus (MrNV) in white tail disease (WTD) of M. rosenbergii. We developed 2 genome-based detection methods for the identification of XSV, namely dot-blot hybridization and a single-step RT-PCR. Detection limits were established and are ca. 2.5 pg and 5 fg of viral RNA for dot-blot hybridization and RT-PCR, respectively. Application of the methods to field samples indicated that some animals positively diagnosed with MrNV did not contain XSV, at least within the detection limit of the methodology. 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| ispartof | Diseases of aquatic organisms, 2004-01, Vol.58 (1), p.83-87 |
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| language | eng |
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| source | MEDLINE; Inter-Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animal aquaculture Animal productions Animals Aquaculture Biological and medical sciences Crustacea DNA Primers DNA Probes extra small virus Freshwater Fundamental and applied biological sciences. Psychology Invertebrate aquaculture Macrobrachium rosenbergii Nodaviridae Nodavirus Palaemonidae - virology Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics |
| title | Dot-blot hybridization and RT-PCR detection of extra small virus (XSV) associated with white tail disease of prawn Macrobrachium rosenbergii |
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