Dynamics of intracellular movement and nucleocytoplasmic recycling of the ligand-activated androgen receptor in living cells

An expression construct containing the cDNA encoding a modified aequorea green fluorescent protein (GFP) ligated to the 5'-end of the rat androgen receptor (AR) cDNA (GFP-AR) was used to study the intracellular dynamics of the receptor movement in living cells. In three different cell lines, ie...

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Veröffentlicht in:	Molecular endocrinology (Baltimore, Md.) Md.), 2000-08, Vol.14 (8), p.1162-1174
Hauptverfasser:	Tyagi, R K, Lavrovsky, Y, Ahn, S C, Song, C S, Chatterjee, B, Roy, A K
Format:	Artikel
Sprache:	eng
Schlagworte:	Androgen Antagonists - pharmacology Anilides - pharmacology Animals Biological Transport, Active - drug effects Cell Compartmentation Cell Line Cell Nucleus - drug effects Cell Nucleus - metabolism Cyproterone - pharmacology Cytoplasm - drug effects Cytoplasm - metabolism Dihydrotestosterone - pharmacology Estradiol - pharmacology Fatty Acids, Unsaturated - pharmacology Green Fluorescent Proteins Humans Kinetics Ligands Luminescent Proteins - genetics Luminescent Proteins - metabolism Nitriles Progesterone - pharmacology Rats Receptors, Androgen - drug effects Receptors, Androgen - genetics Receptors, Androgen - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Tosyl Compounds Transcriptional Activation
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