Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides

The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed...

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Veröffentlicht in:	Rapid communications in mass spectrometry 2000-01, Vol.14 (16), p.1448-1454
Hauptverfasser:	Guc̆ek, Marjan, Gaspari, Marco, Walhagen, Karin, Vreeken, Rob J., Verheij, Elwin R., van der Greef, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography - methods Deamino Arginine Vasopressin - chemistry Deamino Arginine Vasopressin - isolation & purification Enkephalin, Methionine - chemistry Enkephalin, Methionine - isolation & purification Mass Spectrometry - methods Oncogene Protein pp60(v-src) - chemistry Oncogene Protein pp60(v-src) - isolation & purification Oxytocin - analogs & derivatives Oxytocin - chemistry Oxytocin - isolation & purification Peptide Fragments - chemistry Peptide Fragments - isolation & purification Peptides - isolation & purification Quality Control Sensitivity and Specificity
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