Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides
The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed...
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| Veröffentlicht in: | Rapid communications in mass spectrometry 2000-01, Vol.14 (16), p.1448-1454 |
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| container_title | Rapid communications in mass spectrometry |
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| creator | Guc̆ek, Marjan Gaspari, Marco Walhagen, Karin Vreeken, Rob J. Verheij, Elwin R. van der Greef, Jan |
| description | The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with CEC columns (100 µm i.d./25 cm long) packed with 3 µm Hypersil C8 or C18 bonded silica particles with an eluent composed of ammonium acetate/acetonitrile. Peptide mixtures of desmopressin, peptide A, oxytocin, carbetocin and [Met5]‐enkephalin were detected in the mid‐attomole range, which is the lowest amount analyzed using CEC combined with MS detection. It was also observed that sensitivity can be compromised at higher separation voltages. We demonstrate that CEC/nESI‐MS, at the current stage of development, represents one of the most sensitive systems for peptide analysis.Copyright © 2000 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/1097-0231(20000830)14:16<1448::AID-RCM44>3.0.CO;2-9 |
| format | Article |
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Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with CEC columns (100 µm i.d./25 cm long) packed with 3 µm Hypersil C8 or C18 bonded silica particles with an eluent composed of ammonium acetate/acetonitrile. Peptide mixtures of desmopressin, peptide A, oxytocin, carbetocin and [Met5]‐enkephalin were detected in the mid‐attomole range, which is the lowest amount analyzed using CEC combined with MS detection. It was also observed that sensitivity can be compromised at higher separation voltages. We demonstrate that CEC/nESI‐MS, at the current stage of development, represents one of the most sensitive systems for peptide analysis.Copyright © 2000 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-4198</identifier><identifier>EISSN: 1097-0231</identifier><identifier>DOI: 10.1002/1097-0231(20000830)14:16<1448::AID-RCM44>3.0.CO;2-9</identifier><identifier>PMID: 10931536</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject><![CDATA[Amino Acid Sequence ; Chromatography - methods ; Deamino Arginine Vasopressin - chemistry ; Deamino Arginine Vasopressin - isolation & purification ; Enkephalin, Methionine - chemistry ; Enkephalin, Methionine - isolation & purification ; Mass Spectrometry - methods ; Oncogene Protein pp60(v-src) - chemistry ; Oncogene Protein pp60(v-src) - isolation & purification ; Oxytocin - analogs & derivatives ; Oxytocin - chemistry ; Oxytocin - isolation & purification ; Peptide Fragments - chemistry ; Peptide Fragments - isolation & purification ; Peptides - isolation & purification ; Quality Control ; Sensitivity and Specificity]]></subject><ispartof>Rapid communications in mass spectrometry, 2000-01, Vol.14 (16), p.1448-1454</ispartof><rights>Copyright © 2000 John Wiley & Sons, Ltd.</rights><rights>Copyright 2000 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3994-e856cda9f1eeb3cfd856ba16ea7447bbd78a288f131d3fdedc92bcb3f9a44b223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1097-0231%2820000830%2914%3A16%3C1448%3A%3AAID-RCM44%3E3.0.CO%3B2-9$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1097-0231%2820000830%2914%3A16%3C1448%3A%3AAID-RCM44%3E3.0.CO%3B2-9$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10931536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guc̆ek, Marjan</creatorcontrib><creatorcontrib>Gaspari, Marco</creatorcontrib><creatorcontrib>Walhagen, Karin</creatorcontrib><creatorcontrib>Vreeken, Rob J.</creatorcontrib><creatorcontrib>Verheij, Elwin R.</creatorcontrib><creatorcontrib>van der Greef, Jan</creatorcontrib><title>Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides</title><title>Rapid communications in mass spectrometry</title><addtitle>Rapid Commun. Mass Spectrom</addtitle><description>The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with CEC columns (100 µm i.d./25 cm long) packed with 3 µm Hypersil C8 or C18 bonded silica particles with an eluent composed of ammonium acetate/acetonitrile. Peptide mixtures of desmopressin, peptide A, oxytocin, carbetocin and [Met5]‐enkephalin were detected in the mid‐attomole range, which is the lowest amount analyzed using CEC combined with MS detection. It was also observed that sensitivity can be compromised at higher separation voltages. We demonstrate that CEC/nESI‐MS, at the current stage of development, represents one of the most sensitive systems for peptide analysis.Copyright © 2000 John Wiley & Sons, Ltd.</description><subject>Amino Acid Sequence</subject><subject>Chromatography - methods</subject><subject>Deamino Arginine Vasopressin - chemistry</subject><subject>Deamino Arginine Vasopressin - isolation & purification</subject><subject>Enkephalin, Methionine - chemistry</subject><subject>Enkephalin, Methionine - isolation & purification</subject><subject>Mass Spectrometry - methods</subject><subject>Oncogene Protein pp60(v-src) - chemistry</subject><subject>Oncogene Protein pp60(v-src) - isolation & purification</subject><subject>Oxytocin - analogs & derivatives</subject><subject>Oxytocin - chemistry</subject><subject>Oxytocin - isolation & purification</subject><subject>Peptide Fragments - chemistry</subject><subject>Peptide Fragments - isolation & purification</subject><subject>Peptides - isolation & purification</subject><subject>Quality Control</subject><subject>Sensitivity and Specificity</subject><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUU2P0zAUtBCILQt_AeWE4JCuv_LhLkJaBSgrFirxoR6fHOeFBpI62K6g_HrcTak4cMEXy8_zZjQzhFwyOmeU8gtGVZFSLthTTuMpBX3G5ILlz5mU5WJxdf0y_VC9k_KFmNN5tbrkqbpDZqetu2RGVcZSyVR5Rh54_5VSxjJO75OzCBIsE_mM9JUeu77Xbp9gjyY4azbODjrYL06Pm_3FVm_t8cePTu-TQXuf-PF2MmCIi611iQ7BDrbHxGy00yag637p0NltYttkxDF0DfqH5F6re4-Pjvc5-fz61afqTXqzWl5XVzepEUrJFMssN41WLUOshWmb-K41y1EXUhZ13RSl5mXZMsEa0TbYGMVrU4tWaSlrzsU5eTLxjs5-36EPMHTeYLS5RbvzULBCKiFlBH6cgCa68w5bGF03xDCAUTiUAIc44RAn_CkBmASWw6EEgFgC3JYAAihUK-CgIuvjo_yuHrD5i3NKXZx8_-h63P-n5r8kp0HkTSfezgf8eeLV7hvkhSgyWL9fwlLx9Xr9loMUvwHo0LOv</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Guc̆ek, Marjan</creator><creator>Gaspari, Marco</creator><creator>Walhagen, Karin</creator><creator>Vreeken, Rob J.</creator><creator>Verheij, Elwin R.</creator><creator>van der Greef, Jan</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000101</creationdate><title>Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides</title><author>Guc̆ek, Marjan ; Gaspari, Marco ; Walhagen, Karin ; Vreeken, Rob J. ; Verheij, Elwin R. ; van der Greef, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3994-e856cda9f1eeb3cfd856ba16ea7447bbd78a288f131d3fdedc92bcb3f9a44b223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Chromatography - methods</topic><topic>Deamino Arginine Vasopressin - chemistry</topic><topic>Deamino Arginine Vasopressin - isolation & purification</topic><topic>Enkephalin, Methionine - chemistry</topic><topic>Enkephalin, Methionine - isolation & purification</topic><topic>Mass Spectrometry - methods</topic><topic>Oncogene Protein pp60(v-src) - chemistry</topic><topic>Oncogene Protein pp60(v-src) - isolation & purification</topic><topic>Oxytocin - analogs & derivatives</topic><topic>Oxytocin - chemistry</topic><topic>Oxytocin - isolation & purification</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - isolation & purification</topic><topic>Peptides - isolation & purification</topic><topic>Quality Control</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guc̆ek, Marjan</creatorcontrib><creatorcontrib>Gaspari, Marco</creatorcontrib><creatorcontrib>Walhagen, Karin</creatorcontrib><creatorcontrib>Vreeken, Rob J.</creatorcontrib><creatorcontrib>Verheij, Elwin R.</creatorcontrib><creatorcontrib>van der Greef, Jan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guc̆ek, Marjan</au><au>Gaspari, Marco</au><au>Walhagen, Karin</au><au>Vreeken, Rob J.</au><au>Verheij, Elwin R.</au><au>van der Greef, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><addtitle>Rapid Commun. Mass Spectrom</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>14</volume><issue>16</issue><spage>1448</spage><epage>1454</epage><pages>1448-1454</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with CEC columns (100 µm i.d./25 cm long) packed with 3 µm Hypersil C8 or C18 bonded silica particles with an eluent composed of ammonium acetate/acetonitrile. Peptide mixtures of desmopressin, peptide A, oxytocin, carbetocin and [Met5]‐enkephalin were detected in the mid‐attomole range, which is the lowest amount analyzed using CEC combined with MS detection. It was also observed that sensitivity can be compromised at higher separation voltages. We demonstrate that CEC/nESI‐MS, at the current stage of development, represents one of the most sensitive systems for peptide analysis.Copyright © 2000 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>10931536</pmid><doi>10.1002/1097-0231(20000830)14:16<1448::AID-RCM44>3.0.CO;2-9</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Chromatography - methods Deamino Arginine Vasopressin - chemistry Deamino Arginine Vasopressin - isolation & purification Enkephalin, Methionine - chemistry Enkephalin, Methionine - isolation & purification Mass Spectrometry - methods Oncogene Protein pp60(v-src) - chemistry Oncogene Protein pp60(v-src) - isolation & purification Oxytocin - analogs & derivatives Oxytocin - chemistry Oxytocin - isolation & purification Peptide Fragments - chemistry Peptide Fragments - isolation & purification Peptides - isolation & purification Quality Control Sensitivity and Specificity |
| title | Capillary electrochromatography/nanoelectrospray mass spectrometry for attomole characterization of peptides |
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