Role of Reconstituted Basement Membrane in Human Granulosa Cell Culture
The effects of Matrigel, a reconstituted basement membrane, on human granulosa cells were investigated. Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and...
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| Veröffentlicht in: | ENDOCRINE JOURNAL 2000, Vol.47(2), pp.177-183 |
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| container_title | ENDOCRINE JOURNAL |
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| creator | HWANG, DOUK-HO KEE, SOOK-HYEUN KIM, KICHUL CHEONG, KYUNG-SOON YOO, YOUNG-BOK LEE, BYUNG-LAN |
| description | The effects of Matrigel, a reconstituted basement membrane, on human granulosa cells were investigated. Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and electron microscopy showed that granulosa cells cultured on Matrigel demonstrated three-dimensional aggregated cells with well differentiated morphology: numerous lipid droplets, microvilli, junctional complexes and lumen-like structures were seen. In contrast, cells cultured on plastic were flattened, poorly differentiated and showed apoptotic cells. Immunocytochemistry showed that the proportion of immunopositive cells for 3β-hydroxysteroid dehydrogenase was increased in cultures on Matrigel. The results of the present study suggest that culture on Matrigel promotes the differentiation of human granulosa cells and provides a useful tool which may improve the efficiency of in vitro fertilization. |
| doi_str_mv | 10.1507/endocrj.47.177 |
| format | Article |
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Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and electron microscopy showed that granulosa cells cultured on Matrigel demonstrated three-dimensional aggregated cells with well differentiated morphology: numerous lipid droplets, microvilli, junctional complexes and lumen-like structures were seen. In contrast, cells cultured on plastic were flattened, poorly differentiated and showed apoptotic cells. Immunocytochemistry showed that the proportion of immunopositive cells for 3β-hydroxysteroid dehydrogenase was increased in cultures on Matrigel. The results of the present study suggest that culture on Matrigel promotes the differentiation of human granulosa cells and provides a useful tool which may improve the efficiency of in vitro fertilization.</description><identifier>ISSN: 0918-8959</identifier><identifier>EISSN: 1348-4540</identifier><identifier>DOI: 10.1507/endocrj.47.177</identifier><identifier>PMID: 10943742</identifier><language>eng</language><publisher>Japan: The Japan Endocrine Society</publisher><subject>3-Hydroxysteroid Dehydrogenases - analysis ; 3β-Hydroxysteroid dehydrogenase ; Apoptosis ; Cell Adhesion ; Cell Differentiation ; Cells, Cultured ; Collagen - pharmacology ; Culture Media ; Drug Combinations ; Female ; Granulosa cells ; Granulosa Cells - enzymology ; Granulosa Cells - ultrastructure ; Humans ; Immunocytochemistry ; Immunohistochemistry ; Intercellular Junctions - ultrastructure ; Laminin - pharmacology ; Lipids - analysis ; Matrigel ; Microscopy, Electron ; Microvilli - ultrastructure ; Morphology ; Proteoglycans - pharmacology</subject><ispartof>Endocrine Journal, 2000, Vol.47(2), pp.177-183</ispartof><rights>The Japan Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c654t-481df19f9a05c4513dcfa6c575274f2774302fd7959410c251acd1c6dfed1ed93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1881,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10943742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HWANG, DOUK-HO</creatorcontrib><creatorcontrib>KEE, SOOK-HYEUN</creatorcontrib><creatorcontrib>KIM, KICHUL</creatorcontrib><creatorcontrib>CHEONG, KYUNG-SOON</creatorcontrib><creatorcontrib>YOO, YOUNG-BOK</creatorcontrib><creatorcontrib>LEE, BYUNG-LAN</creatorcontrib><creatorcontrib>College of Medicine</creatorcontrib><creatorcontrib>Department of Anatomy</creatorcontrib><creatorcontrib>Seoul National University</creatorcontrib><creatorcontrib>Dankook University</creatorcontrib><creatorcontrib>Hamchoon Women's Clinic</creatorcontrib><title>Role of Reconstituted Basement Membrane in Human Granulosa Cell Culture</title><title>ENDOCRINE JOURNAL</title><addtitle>Endocr J</addtitle><description>The effects of Matrigel, a reconstituted basement membrane, on human granulosa cells were investigated. Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and electron microscopy showed that granulosa cells cultured on Matrigel demonstrated three-dimensional aggregated cells with well differentiated morphology: numerous lipid droplets, microvilli, junctional complexes and lumen-like structures were seen. In contrast, cells cultured on plastic were flattened, poorly differentiated and showed apoptotic cells. Immunocytochemistry showed that the proportion of immunopositive cells for 3β-hydroxysteroid dehydrogenase was increased in cultures on Matrigel. The results of the present study suggest that culture on Matrigel promotes the differentiation of human granulosa cells and provides a useful tool which may improve the efficiency of in vitro fertilization.</description><subject>3-Hydroxysteroid Dehydrogenases - analysis</subject><subject>3β-Hydroxysteroid dehydrogenase</subject><subject>Apoptosis</subject><subject>Cell Adhesion</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Collagen - pharmacology</subject><subject>Culture Media</subject><subject>Drug Combinations</subject><subject>Female</subject><subject>Granulosa cells</subject><subject>Granulosa Cells - enzymology</subject><subject>Granulosa Cells - ultrastructure</subject><subject>Humans</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>Intercellular Junctions - ultrastructure</subject><subject>Laminin - pharmacology</subject><subject>Lipids - analysis</subject><subject>Matrigel</subject><subject>Microscopy, Electron</subject><subject>Microvilli - ultrastructure</subject><subject>Morphology</subject><subject>Proteoglycans - pharmacology</subject><issn>0918-8959</issn><issn>1348-4540</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEFvFCEYhomxsdvq1aPh5G22HwMMw1E3dtukpkmjZ0LhQ2czAy3MHPz3sp2t8QIhPDzfy0vIRwZbJkFdYfTJ5cNWqC1T6g3ZMC76RkgBb8kGNOubXkt9Ti5KOQBwLgV_R84ZaMGVaDdk_5BGpCnQB3QplnmYlxk9_WoLThhn-h2nx2wj0iHSm2Wyke7rcRlTsXSH40h3yzgvGd-Ts2DHgh9O-yX5ef3tx-6mubvf3-6-3DWuk2JuRM98YDpoC9IJybh3wXZOKtkqEVqlBIc2eFUzCwaulcw6z1znA3qGXvNL8nn1PuX0vGCZzTQUV4PUjGkpRjElFJNdBbcr6HIqJWMwT3mYbP5jGJhjdeZUnRHK1Orqg08n8_I4of8PX7uqwPUK1NvB2THFcYhoDmnJsX7ZuOfuRWlaADAAQkFroE6Dqq9LX9vvVN9X0X4VHcpsf-G_STbPgxvxNRjTmh_DtetylLwS7rfNFeN_AWMQmqQ</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>HWANG, DOUK-HO</creator><creator>KEE, SOOK-HYEUN</creator><creator>KIM, KICHUL</creator><creator>CHEONG, KYUNG-SOON</creator><creator>YOO, YOUNG-BOK</creator><creator>LEE, BYUNG-LAN</creator><general>The Japan Endocrine Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Role of Reconstituted Basement Membrane in Human Granulosa Cell Culture</title><author>HWANG, DOUK-HO ; KEE, SOOK-HYEUN ; KIM, KICHUL ; CHEONG, KYUNG-SOON ; YOO, YOUNG-BOK ; LEE, BYUNG-LAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c654t-481df19f9a05c4513dcfa6c575274f2774302fd7959410c251acd1c6dfed1ed93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>3-Hydroxysteroid Dehydrogenases - analysis</topic><topic>3β-Hydroxysteroid dehydrogenase</topic><topic>Apoptosis</topic><topic>Cell Adhesion</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Collagen - pharmacology</topic><topic>Culture Media</topic><topic>Drug Combinations</topic><topic>Female</topic><topic>Granulosa cells</topic><topic>Granulosa Cells - enzymology</topic><topic>Granulosa Cells - ultrastructure</topic><topic>Humans</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>Intercellular Junctions - ultrastructure</topic><topic>Laminin - pharmacology</topic><topic>Lipids - analysis</topic><topic>Matrigel</topic><topic>Microscopy, Electron</topic><topic>Microvilli - ultrastructure</topic><topic>Morphology</topic><topic>Proteoglycans - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HWANG, DOUK-HO</creatorcontrib><creatorcontrib>KEE, SOOK-HYEUN</creatorcontrib><creatorcontrib>KIM, KICHUL</creatorcontrib><creatorcontrib>CHEONG, KYUNG-SOON</creatorcontrib><creatorcontrib>YOO, YOUNG-BOK</creatorcontrib><creatorcontrib>LEE, BYUNG-LAN</creatorcontrib><creatorcontrib>College of Medicine</creatorcontrib><creatorcontrib>Department of Anatomy</creatorcontrib><creatorcontrib>Seoul National University</creatorcontrib><creatorcontrib>Dankook University</creatorcontrib><creatorcontrib>Hamchoon Women's Clinic</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ENDOCRINE JOURNAL</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HWANG, DOUK-HO</au><au>KEE, SOOK-HYEUN</au><au>KIM, KICHUL</au><au>CHEONG, KYUNG-SOON</au><au>YOO, YOUNG-BOK</au><au>LEE, BYUNG-LAN</au><aucorp>College of Medicine</aucorp><aucorp>Department of Anatomy</aucorp><aucorp>Seoul National University</aucorp><aucorp>Dankook University</aucorp><aucorp>Hamchoon Women's Clinic</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Reconstituted Basement Membrane in Human Granulosa Cell Culture</atitle><jtitle>ENDOCRINE JOURNAL</jtitle><addtitle>Endocr J</addtitle><date>2000</date><risdate>2000</risdate><volume>47</volume><issue>2</issue><spage>177</spage><epage>183</epage><pages>177-183</pages><issn>0918-8959</issn><eissn>1348-4540</eissn><abstract>The effects of Matrigel, a reconstituted basement membrane, on human granulosa cells were investigated. Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and electron microscopy showed that granulosa cells cultured on Matrigel demonstrated three-dimensional aggregated cells with well differentiated morphology: numerous lipid droplets, microvilli, junctional complexes and lumen-like structures were seen. In contrast, cells cultured on plastic were flattened, poorly differentiated and showed apoptotic cells. Immunocytochemistry showed that the proportion of immunopositive cells for 3β-hydroxysteroid dehydrogenase was increased in cultures on Matrigel. The results of the present study suggest that culture on Matrigel promotes the differentiation of human granulosa cells and provides a useful tool which may improve the efficiency of in vitro fertilization.</abstract><cop>Japan</cop><pub>The Japan Endocrine Society</pub><pmid>10943742</pmid><doi>10.1507/endocrj.47.177</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3-Hydroxysteroid Dehydrogenases - analysis 3β-Hydroxysteroid dehydrogenase Apoptosis Cell Adhesion Cell Differentiation Cells, Cultured Collagen - pharmacology Culture Media Drug Combinations Female Granulosa cells Granulosa Cells - enzymology Granulosa Cells - ultrastructure Humans Immunocytochemistry Immunohistochemistry Intercellular Junctions - ultrastructure Laminin - pharmacology Lipids - analysis Matrigel Microscopy, Electron Microvilli - ultrastructure Morphology Proteoglycans - pharmacology |
| title | Role of Reconstituted Basement Membrane in Human Granulosa Cell Culture |
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