Voltage activation and hysteresis of the non-selective voltage-dependent channel in the intact human red cell
Suspension of intact human red cells in media with low chloride and sodium concentrations (isotonic sucrose substitution) results in strongly inside positive membrane potentials, which activate the voltage-dependent non-selective cation (NSVDC) channel. By systematic variation of the initial Nernst...
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| Veröffentlicht in: | Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2004-05, Vol.62 (2), p.181-185 |
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| container_title | Bioelectrochemistry (Amsterdam, Netherlands) |
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| creator | Bennekou, Poul Barksmann, Trine L. Jensen, Lars R. Kristensen, Berit I. Christophersen, Palle |
| description | Suspension of intact human red cells in media with low chloride and sodium concentrations (isotonic sucrose substitution) results in strongly inside positive membrane potentials, which activate the voltage-dependent non-selective cation (NSVDC) channel. By systematic variation of the initial Nernst potentials for chloride (degree of ion substitution) as well as the chloride conductance (block by NS1652), and by exploiting the interplay between the Ca
2+-permeable NSVDC channel, the Ca
2+-activated K
+ channel (the Gárdos channel) and the Ca
2+-pump, a graded activation of the NSVDC channel was achieved. Under these conditions, it was shown that the NSVDC channels exist in two states of activation depending on the initial conditions for the activation. The hysteretic behaviour, which in patch clamp experiments has been found for the individual channel unit, is thus retained at the cellular level and can be demonstrated with red cells in suspension. |
| doi_str_mv | 10.1016/j.bioelechem.2003.08.006 |
| format | Article |
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2+-permeable NSVDC channel, the Ca
2+-activated K
+ channel (the Gárdos channel) and the Ca
2+-pump, a graded activation of the NSVDC channel was achieved. Under these conditions, it was shown that the NSVDC channels exist in two states of activation depending on the initial conditions for the activation. The hysteretic behaviour, which in patch clamp experiments has been found for the individual channel unit, is thus retained at the cellular level and can be demonstrated with red cells in suspension.</description><identifier>ISSN: 1567-5394</identifier><identifier>EISSN: 1878-562X</identifier><identifier>DOI: 10.1016/j.bioelechem.2003.08.006</identifier><identifier>PMID: 15039024</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Ca 2+-transient ; Calcium Channels - metabolism ; Calcium Channels - physiology ; Calcium-Transporting ATPases - metabolism ; Calcium-Transporting ATPases - physiology ; Electrophysiology ; Erythrocytes - physiology ; Human red cells ; Humans ; Hysteresis ; Ion Channels - metabolism ; Ion Channels - physiology ; Membrane Potentials ; Non-selective voltage-dependent cation channel ; Patch-Clamp Techniques ; Potassium Channels, Calcium-Activated - metabolism ; Potassium Channels, Calcium-Activated - physiology</subject><ispartof>Bioelectrochemistry (Amsterdam, Netherlands), 2004-05, Vol.62 (2), p.181-185</ispartof><rights>2003 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-c0b4e6ac670f32a076e85b385586f2cdd3092a53080042c44ff412c44c6c3b53</citedby><cites>FETCH-LOGICAL-c370t-c0b4e6ac670f32a076e85b385586f2cdd3092a53080042c44ff412c44c6c3b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1567539403001002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15039024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bennekou, Poul</creatorcontrib><creatorcontrib>Barksmann, Trine L.</creatorcontrib><creatorcontrib>Jensen, Lars R.</creatorcontrib><creatorcontrib>Kristensen, Berit I.</creatorcontrib><creatorcontrib>Christophersen, Palle</creatorcontrib><title>Voltage activation and hysteresis of the non-selective voltage-dependent channel in the intact human red cell</title><title>Bioelectrochemistry (Amsterdam, Netherlands)</title><addtitle>Bioelectrochemistry</addtitle><description>Suspension of intact human red cells in media with low chloride and sodium concentrations (isotonic sucrose substitution) results in strongly inside positive membrane potentials, which activate the voltage-dependent non-selective cation (NSVDC) channel. By systematic variation of the initial Nernst potentials for chloride (degree of ion substitution) as well as the chloride conductance (block by NS1652), and by exploiting the interplay between the Ca
2+-permeable NSVDC channel, the Ca
2+-activated K
+ channel (the Gárdos channel) and the Ca
2+-pump, a graded activation of the NSVDC channel was achieved. Under these conditions, it was shown that the NSVDC channels exist in two states of activation depending on the initial conditions for the activation. The hysteretic behaviour, which in patch clamp experiments has been found for the individual channel unit, is thus retained at the cellular level and can be demonstrated with red cells in suspension.</description><subject>Ca 2+-transient</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Channels - physiology</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Calcium-Transporting ATPases - physiology</subject><subject>Electrophysiology</subject><subject>Erythrocytes - physiology</subject><subject>Human red cells</subject><subject>Humans</subject><subject>Hysteresis</subject><subject>Ion Channels - metabolism</subject><subject>Ion Channels - physiology</subject><subject>Membrane Potentials</subject><subject>Non-selective voltage-dependent cation channel</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channels, Calcium-Activated - metabolism</subject><subject>Potassium Channels, Calcium-Activated - physiology</subject><issn>1567-5394</issn><issn>1878-562X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2PFCEQhonRuB_6Fwwnb91bNA3NHHXjqskmXjbGG6Gh2mbSDSMwk-y_l3Ym2aMHUhyet97UQwhl0DJg8m7fjj7ignbGte0AeAuqBZCvyDVTg2qE7H69rn8hh0bwXX9FbnLeA4Big3hLrpgAvoOuvybrz7gU8xupscWfTPExUBMcnZ9zwYTZZxonWmakIYYmb52VQ3o6xxqHBwwOQ6F2NiHgQn34h_tQ6ko6H1cTaEJHLS7LO_JmMkvG95d5S54evjzdf2sef3z9fv_psbF8gNJYGHuUxsoBJt4ZGCQqMXIlhJJTZ53jsOuM4KAA-s72_TT1bJtWWj4Kfks-ntceUvxzxFz06vPWbwLGY9YDG3pZXwXVGbQp5pxw0ofkV5OeNQO9mdZ7_WJab6Y1KF1N1-iHS8dxXNG9BC9qK_D5DGA99OQx6Ww9BovOpypRu-j_3_IXGcKVxQ</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Bennekou, Poul</creator><creator>Barksmann, Trine L.</creator><creator>Jensen, Lars R.</creator><creator>Kristensen, Berit I.</creator><creator>Christophersen, Palle</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>Voltage activation and hysteresis of the non-selective voltage-dependent channel in the intact human red cell</title><author>Bennekou, Poul ; Barksmann, Trine L. ; Jensen, Lars R. ; Kristensen, Berit I. ; Christophersen, Palle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-c0b4e6ac670f32a076e85b385586f2cdd3092a53080042c44ff412c44c6c3b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Ca 2+-transient</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Channels - physiology</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Calcium-Transporting ATPases - physiology</topic><topic>Electrophysiology</topic><topic>Erythrocytes - physiology</topic><topic>Human red cells</topic><topic>Humans</topic><topic>Hysteresis</topic><topic>Ion Channels - metabolism</topic><topic>Ion Channels - physiology</topic><topic>Membrane Potentials</topic><topic>Non-selective voltage-dependent cation channel</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channels, Calcium-Activated - metabolism</topic><topic>Potassium Channels, Calcium-Activated - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bennekou, Poul</creatorcontrib><creatorcontrib>Barksmann, Trine L.</creatorcontrib><creatorcontrib>Jensen, Lars R.</creatorcontrib><creatorcontrib>Kristensen, Berit I.</creatorcontrib><creatorcontrib>Christophersen, Palle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bennekou, Poul</au><au>Barksmann, Trine L.</au><au>Jensen, Lars R.</au><au>Kristensen, Berit I.</au><au>Christophersen, Palle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Voltage activation and hysteresis of the non-selective voltage-dependent channel in the intact human red cell</atitle><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle><addtitle>Bioelectrochemistry</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>62</volume><issue>2</issue><spage>181</spage><epage>185</epage><pages>181-185</pages><issn>1567-5394</issn><eissn>1878-562X</eissn><abstract>Suspension of intact human red cells in media with low chloride and sodium concentrations (isotonic sucrose substitution) results in strongly inside positive membrane potentials, which activate the voltage-dependent non-selective cation (NSVDC) channel. By systematic variation of the initial Nernst potentials for chloride (degree of ion substitution) as well as the chloride conductance (block by NS1652), and by exploiting the interplay between the Ca
2+-permeable NSVDC channel, the Ca
2+-activated K
+ channel (the Gárdos channel) and the Ca
2+-pump, a graded activation of the NSVDC channel was achieved. Under these conditions, it was shown that the NSVDC channels exist in two states of activation depending on the initial conditions for the activation. The hysteretic behaviour, which in patch clamp experiments has been found for the individual channel unit, is thus retained at the cellular level and can be demonstrated with red cells in suspension.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15039024</pmid><doi>10.1016/j.bioelechem.2003.08.006</doi><tpages>5</tpages></addata></record> |
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| ispartof | Bioelectrochemistry (Amsterdam, Netherlands), 2004-05, Vol.62 (2), p.181-185 |
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| language | eng |
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| subjects | Ca 2+-transient Calcium Channels - metabolism Calcium Channels - physiology Calcium-Transporting ATPases - metabolism Calcium-Transporting ATPases - physiology Electrophysiology Erythrocytes - physiology Human red cells Humans Hysteresis Ion Channels - metabolism Ion Channels - physiology Membrane Potentials Non-selective voltage-dependent cation channel Patch-Clamp Techniques Potassium Channels, Calcium-Activated - metabolism Potassium Channels, Calcium-Activated - physiology |
| title | Voltage activation and hysteresis of the non-selective voltage-dependent channel in the intact human red cell |
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