Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish
A transforming growth factor (TGF)-β was isolated and cloned from hybrid striped bass (Morone saxatilis x M. chrysops) anterior kidney mononuclear cells. This isolate (Genbank accession number AF140363) contains an open reading frame of 1146 bases coding for a 382 amino acid protein most similar to...
Gespeichert in:
| Veröffentlicht in: | Fish & shellfish immunology 2000-01, Vol.10 (1), p.61-85 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 85 |
|---|---|
| container_issue | 1 |
| container_start_page | 61 |
| container_title | Fish & shellfish immunology |
| container_volume | 10 |
| creator | Harms, C.A Kennedy-Stoskopf, S Horne, W.A Fuller, F.J Tompkins, W.A.F |
| description | A transforming growth factor (TGF)-β was isolated and cloned from hybrid striped bass (Morone saxatilis x M. chrysops) anterior kidney mononuclear cells. This isolate (Genbank accession number AF140363) contains an open reading frame of 1146 bases coding for a 382 amino acid protein most similar to rainbow trout TGF-β (57·3 and 78·6% identity with precursor and active protein, respectively) and rat TGF-β1(41·1 and 68·8% identity with precursor and active protein, respectively). Consensus primers were demonstrated to amplify specifically by polymerase chain reaction (PCR), a TGF-β segment from 14 species of teleost fish comprising 10 taxonomic families in 7 orders. A reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay was devised to measure TGF-β mRNA expression in teleost fish. Higher levels of TGF-β mRNA expression were detected in mononuclear cells of peripheral blood than from spleen or anterior kidney. |
| doi_str_mv | 10.1006/fsim.1999.0230 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71743760</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1050464899902309</els_id><sourcerecordid>71743760</sourcerecordid><originalsourceid>FETCH-LOGICAL-c371t-86e37b2ea63967c032284249ba22c6fcb6af8b8eba1863c11770cbd6826682143</originalsourceid><addsrcrecordid>eNqFUs2O0zAQDgjEloUrRzSnVSuRYsepnRxXFbuLtAuoKufIcSZbo8RObbdsX4sH4ZnWIXvggjhY4xl9P6PRlyTvKFlSQvjH1ut-ScuyXJKMkefJjJJylZZlLl6M_xVJc54XZ8lr73-QSGCcvErOIogVImOzZ7N1Z4029yBNAx73BzRqbHen2uk4CU4P2EAtvYf5nXXWIHj5IIPutIcHuFuC2rmTt4NfQHDS-Na6flS4d_Zn2EErVbAu_f0L5tvrq1gXH_54NXjEzg49mgC2BQkuDpzHSURF26Ctgf1BmqBD9DsiKNsPGLvxP9ju1KOTkaF2UpvIj04jZb7Zpnv1bb1ZQNxaniBY6FH6g0OYVoB-8-VydA3YofUBWu13b5KXrew8vn2q58n3q0_b9U16-_X68_ryNlVM0JAWHJmoM5SclVwowrKsyLO8rGWWKd6qmsu2qAusJS04U5QKQVTd8CLj8dGcnScXk-7gbDy3D1WvvcKukwbtwVeCipwJTv4LpAURqxUTEbicgMpZ7x221eB0L92poqQaU1KNKanGlFRjSiLh_ZPyoe6x-Qs-xSICigmA8RBHja7ySsdkYKMdqlA1Vv9L-xFKI9H3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18075537</pqid></control><display><type>article</type><title>Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Harms, C.A ; Kennedy-Stoskopf, S ; Horne, W.A ; Fuller, F.J ; Tompkins, W.A.F</creator><creatorcontrib>Harms, C.A ; Kennedy-Stoskopf, S ; Horne, W.A ; Fuller, F.J ; Tompkins, W.A.F</creatorcontrib><description>A transforming growth factor (TGF)-β was isolated and cloned from hybrid striped bass (Morone saxatilis x M. chrysops) anterior kidney mononuclear cells. This isolate (Genbank accession number AF140363) contains an open reading frame of 1146 bases coding for a 382 amino acid protein most similar to rainbow trout TGF-β (57·3 and 78·6% identity with precursor and active protein, respectively) and rat TGF-β1(41·1 and 68·8% identity with precursor and active protein, respectively). Consensus primers were demonstrated to amplify specifically by polymerase chain reaction (PCR), a TGF-β segment from 14 species of teleost fish comprising 10 taxonomic families in 7 orders. A reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay was devised to measure TGF-β mRNA expression in teleost fish. Higher levels of TGF-β mRNA expression were detected in mononuclear cells of peripheral blood than from spleen or anterior kidney.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1006/fsim.1999.0230</identifier><identifier>PMID: 10938723</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Bass - metabolism ; Blotting, Northern - veterinary ; Blotting, Southern - veterinary ; Chimera - genetics ; Cloning, Molecular ; Consensus Sequence ; Humans ; Kidney - chemistry ; Molecular Sequence Data ; Morone chrysops ; Morone saxatilis ; Oncorhynchus mykiss ; Rats ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - analysis ; Teleostei ; Transforming Growth Factor beta - genetics ; transforming growth factor- beta ; transforming growth factor-β, TGF-β, sequence, hybrid striped bass, Morone saxatilis X M. chrysops, quantitative PCR ; Xenopus</subject><ispartof>Fish & shellfish immunology, 2000-01, Vol.10 (1), p.61-85</ispartof><rights>2000 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-86e37b2ea63967c032284249ba22c6fcb6af8b8eba1863c11770cbd6826682143</citedby><cites>FETCH-LOGICAL-c371t-86e37b2ea63967c032284249ba22c6fcb6af8b8eba1863c11770cbd6826682143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/fsim.1999.0230$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10938723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harms, C.A</creatorcontrib><creatorcontrib>Kennedy-Stoskopf, S</creatorcontrib><creatorcontrib>Horne, W.A</creatorcontrib><creatorcontrib>Fuller, F.J</creatorcontrib><creatorcontrib>Tompkins, W.A.F</creatorcontrib><title>Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>A transforming growth factor (TGF)-β was isolated and cloned from hybrid striped bass (Morone saxatilis x M. chrysops) anterior kidney mononuclear cells. This isolate (Genbank accession number AF140363) contains an open reading frame of 1146 bases coding for a 382 amino acid protein most similar to rainbow trout TGF-β (57·3 and 78·6% identity with precursor and active protein, respectively) and rat TGF-β1(41·1 and 68·8% identity with precursor and active protein, respectively). Consensus primers were demonstrated to amplify specifically by polymerase chain reaction (PCR), a TGF-β segment from 14 species of teleost fish comprising 10 taxonomic families in 7 orders. A reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay was devised to measure TGF-β mRNA expression in teleost fish. Higher levels of TGF-β mRNA expression were detected in mononuclear cells of peripheral blood than from spleen or anterior kidney.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Bass - metabolism</subject><subject>Blotting, Northern - veterinary</subject><subject>Blotting, Southern - veterinary</subject><subject>Chimera - genetics</subject><subject>Cloning, Molecular</subject><subject>Consensus Sequence</subject><subject>Humans</subject><subject>Kidney - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Morone chrysops</subject><subject>Morone saxatilis</subject><subject>Oncorhynchus mykiss</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - analysis</subject><subject>Teleostei</subject><subject>Transforming Growth Factor beta - genetics</subject><subject>transforming growth factor- beta</subject><subject>transforming growth factor-β, TGF-β, sequence, hybrid striped bass, Morone saxatilis X M. chrysops, quantitative PCR</subject><subject>Xenopus</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUs2O0zAQDgjEloUrRzSnVSuRYsepnRxXFbuLtAuoKufIcSZbo8RObbdsX4sH4ZnWIXvggjhY4xl9P6PRlyTvKFlSQvjH1ut-ScuyXJKMkefJjJJylZZlLl6M_xVJc54XZ8lr73-QSGCcvErOIogVImOzZ7N1Z4029yBNAx73BzRqbHen2uk4CU4P2EAtvYf5nXXWIHj5IIPutIcHuFuC2rmTt4NfQHDS-Na6flS4d_Zn2EErVbAu_f0L5tvrq1gXH_54NXjEzg49mgC2BQkuDpzHSURF26Ctgf1BmqBD9DsiKNsPGLvxP9ju1KOTkaF2UpvIj04jZb7Zpnv1bb1ZQNxaniBY6FH6g0OYVoB-8-VydA3YofUBWu13b5KXrew8vn2q58n3q0_b9U16-_X68_ryNlVM0JAWHJmoM5SclVwowrKsyLO8rGWWKd6qmsu2qAusJS04U5QKQVTd8CLj8dGcnScXk-7gbDy3D1WvvcKukwbtwVeCipwJTv4LpAURqxUTEbicgMpZ7x221eB0L92poqQaU1KNKanGlFRjSiLh_ZPyoe6x-Qs-xSICigmA8RBHja7ySsdkYKMdqlA1Vv9L-xFKI9H3</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Harms, C.A</creator><creator>Kennedy-Stoskopf, S</creator><creator>Horne, W.A</creator><creator>Fuller, F.J</creator><creator>Tompkins, W.A.F</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish</title><author>Harms, C.A ; Kennedy-Stoskopf, S ; Horne, W.A ; Fuller, F.J ; Tompkins, W.A.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-86e37b2ea63967c032284249ba22c6fcb6af8b8eba1863c11770cbd6826682143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Bass - metabolism</topic><topic>Blotting, Northern - veterinary</topic><topic>Blotting, Southern - veterinary</topic><topic>Chimera - genetics</topic><topic>Cloning, Molecular</topic><topic>Consensus Sequence</topic><topic>Humans</topic><topic>Kidney - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Morone chrysops</topic><topic>Morone saxatilis</topic><topic>Oncorhynchus mykiss</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - analysis</topic><topic>Teleostei</topic><topic>Transforming Growth Factor beta - genetics</topic><topic>transforming growth factor- beta</topic><topic>transforming growth factor-β, TGF-β, sequence, hybrid striped bass, Morone saxatilis X M. chrysops, quantitative PCR</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harms, C.A</creatorcontrib><creatorcontrib>Kennedy-Stoskopf, S</creatorcontrib><creatorcontrib>Horne, W.A</creatorcontrib><creatorcontrib>Fuller, F.J</creatorcontrib><creatorcontrib>Tompkins, W.A.F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harms, C.A</au><au>Kennedy-Stoskopf, S</au><au>Horne, W.A</au><au>Fuller, F.J</au><au>Tompkins, W.A.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2000-01</date><risdate>2000</risdate><volume>10</volume><issue>1</issue><spage>61</spage><epage>85</epage><pages>61-85</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>A transforming growth factor (TGF)-β was isolated and cloned from hybrid striped bass (Morone saxatilis x M. chrysops) anterior kidney mononuclear cells. This isolate (Genbank accession number AF140363) contains an open reading frame of 1146 bases coding for a 382 amino acid protein most similar to rainbow trout TGF-β (57·3 and 78·6% identity with precursor and active protein, respectively) and rat TGF-β1(41·1 and 68·8% identity with precursor and active protein, respectively). Consensus primers were demonstrated to amplify specifically by polymerase chain reaction (PCR), a TGF-β segment from 14 species of teleost fish comprising 10 taxonomic families in 7 orders. A reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay was devised to measure TGF-β mRNA expression in teleost fish. Higher levels of TGF-β mRNA expression were detected in mononuclear cells of peripheral blood than from spleen or anterior kidney.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>10938723</pmid><doi>10.1006/fsim.1999.0230</doi><tpages>25</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1050-4648 |
| ispartof | Fish & shellfish immunology, 2000-01, Vol.10 (1), p.61-85 |
| issn | 1050-4648 1095-9947 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71743760 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence Animals Base Sequence Bass - metabolism Blotting, Northern - veterinary Blotting, Southern - veterinary Chimera - genetics Cloning, Molecular Consensus Sequence Humans Kidney - chemistry Molecular Sequence Data Morone chrysops Morone saxatilis Oncorhynchus mykiss Rats Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - analysis Teleostei Transforming Growth Factor beta - genetics transforming growth factor- beta transforming growth factor-β, TGF-β, sequence, hybrid striped bass, Morone saxatilis X M. chrysops, quantitative PCR Xenopus |
| title | Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T12%3A06%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20and%20sequencing%20hybrid%20striped%20bass%20(Morone%20saxatilis%20x%20M.%20chrysops)%20transforming%20growth%20factor-%CE%B2%20(TGF-%CE%B2),%20and%20development%20of%20a%20reverse%20transcription%20quantitative%20competitive%20polymerase%20chain%20reaction%20(RT-qcPCR)%20assay%20to%20measure%20TGF-%CE%B2%20mRNA%20of%20teleost%20fish&rft.jtitle=Fish%20&%20shellfish%20immunology&rft.au=Harms,%20C.A&rft.date=2000-01&rft.volume=10&rft.issue=1&rft.spage=61&rft.epage=85&rft.pages=61-85&rft.issn=1050-4648&rft.eissn=1095-9947&rft_id=info:doi/10.1006/fsim.1999.0230&rft_dat=%3Cproquest_cross%3E71743760%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18075537&rft_id=info:pmid/10938723&rft_els_id=S1050464899902309&rfr_iscdi=true |