Calorimetric Demonstration of the Potential of Molecular Crowding To Emulate the Effect of an Allosteric Activator on Pyruvate Kinase Kinetics
A method based on isothermal calorimetry is described for the direct kinetic assay of pyruvate kinase. In agreement with earlier findings based on the standard coupled assay system for this enzyme in the presence of a fixed ADP concentration, the essentially rectangular hyperbolic dependence of init...
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| Veröffentlicht in: | Biochemistry (Easton) 2002-06, Vol.41 (22), p.6897-6901 |
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| container_title | Biochemistry (Easton) |
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| creator | Lonhienne, Thierry G. A Winzor, Donald J |
| description | A method based on isothermal calorimetry is described for the direct kinetic assay of pyruvate kinase. In agreement with earlier findings based on the standard coupled assay system for this enzyme in the presence of a fixed ADP concentration, the essentially rectangular hyperbolic dependence of initial velocity upon phosphoenolpyruvate concentration is rendered sigmoidal by the allosteric inhibitor phenylalanine. This effect of phenylalanine can be countered by including a high concentration of a space-filling osmolyte such as proline in the reaction mixtures. This investigation thus affords a dramatic example that illustrates the need to consider potential consequences of thermodynamic nonideality on the kinetics of enzyme reactions in crowded molecular environments such as the cell cytoplasm. |
| doi_str_mv | 10.1021/bi020064h |
| format | Article |
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This investigation thus affords a dramatic example that illustrates the need to consider potential consequences of thermodynamic nonideality on the kinetics of enzyme reactions in crowded molecular environments such as the cell cytoplasm.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi020064h</identifier><identifier>PMID: 12033921</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Allosteric Regulation - physiology ; Animals ; Calorimetry - methods ; Enzyme Activation - drug effects ; Kinetics ; Muscles - enzymology ; Osmolar Concentration ; Phenylalanine - pharmacology ; Pyruvate Kinase - antagonists & inhibitors ; Pyruvate Kinase - metabolism ; Rabbits ; Thermodynamics</subject><ispartof>Biochemistry (Easton), 2002-06, Vol.41 (22), p.6897-6901</ispartof><rights>Copyright © 2002 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a349t-5921dda59351529c03c6191450c5ee5047ba5166c01a630fa65f8209805efe5f3</citedby><cites>FETCH-LOGICAL-a349t-5921dda59351529c03c6191450c5ee5047ba5166c01a630fa65f8209805efe5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi020064h$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi020064h$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12033921$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lonhienne, Thierry G. 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This investigation thus affords a dramatic example that illustrates the need to consider potential consequences of thermodynamic nonideality on the kinetics of enzyme reactions in crowded molecular environments such as the cell cytoplasm.</description><subject>Allosteric Regulation - physiology</subject><subject>Animals</subject><subject>Calorimetry - methods</subject><subject>Enzyme Activation - drug effects</subject><subject>Kinetics</subject><subject>Muscles - enzymology</subject><subject>Osmolar Concentration</subject><subject>Phenylalanine - pharmacology</subject><subject>Pyruvate Kinase - antagonists & inhibitors</subject><subject>Pyruvate Kinase - metabolism</subject><subject>Rabbits</subject><subject>Thermodynamics</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM1uEzEYRS0EoqGw4AWQNyCxGLDHPxMvozT8NYhIDWwtx_lMXTzjYnsKfQmeGU8TlQ2rT74-vrYPQs8peUNJS9_uPGkJkfzyAZpR0ZKGKyUeohmpYdMqSU7Qk5yv6pKTjj9GJ7QljKmWztCfpQkx-R5K8hafQR-HXJIpPg44OlwuAW9igaF4E6bgcwxgx2ASXqb4a--H73gb8aqvUYE7fOUc2DKxZsCLEGIuMHUvbPE3psSEa_XmNo0304lzP5h8N6B4m5-iR86EDM-O8xR9fbfaLj806y_vPy4X68Ywrkoj6tv3eyMUE_W_yhJmJVWUC2IFgCC82xlBpbSEGsmIM1K4eUvUnAhwIBw7Ra8Ovdcp_hwhF937bCEEM0Acs-5oxxmXqoKvD6BNMecETl9XWybdakr0JF_fy6_si2PpuOth_4882q5AcwB8dfL7ft-kH1p2rBN6u7nQn9YX_Lz7dqbnlX954I3N-iqOaahO_nPxXyIEmw8</recordid><startdate>20020604</startdate><enddate>20020604</enddate><creator>Lonhienne, Thierry G. 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| ispartof | Biochemistry (Easton), 2002-06, Vol.41 (22), p.6897-6901 |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Allosteric Regulation - physiology Animals Calorimetry - methods Enzyme Activation - drug effects Kinetics Muscles - enzymology Osmolar Concentration Phenylalanine - pharmacology Pyruvate Kinase - antagonists & inhibitors Pyruvate Kinase - metabolism Rabbits Thermodynamics |
| title | Calorimetric Demonstration of the Potential of Molecular Crowding To Emulate the Effect of an Allosteric Activator on Pyruvate Kinase Kinetics |
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