Resumption of follicle growth in gilts after ovarian autografting
The aims of the study were to evaluate autografting of porcine ovarian tissue in terms of establishment of a blood supply, follicle survival and development, commencement of oestrous cycles and endocrine patterns in this polyovular species. Experiment 1, a preliminary study on four gilts, showed tha...
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| Veröffentlicht in: | Animal reproduction science 2004-02, Vol.80 (3), p.317-328 |
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| creator | Hunter, M.G. Hudson, N. Mitchell, M. Walker, R.M. Webb, R. |
| description | The aims of the study were to evaluate autografting of porcine ovarian tissue in terms of establishment of a blood supply, follicle survival and development, commencement of oestrous cycles and endocrine patterns in this polyovular species. Experiment 1, a preliminary study on four gilts, showed that ovarian tissue slices survived the grafting procedure and re-vascularised. In Experiment 2, a further six pre-pubertal gilts had both ovaries surgically removed and two thin cortical slices of each ovary were immediately reattached to each of the ovarian pedicles. Blood samples were taken at surgery and then weekly. Two gilts were slaughtered 2 weeks after surgery and ovarian tissue recovered. The remaining four gilts underwent daily checks for behavioural oestrus until slaughter 24 weeks after surgery. All four gilts showed standing heat at least once prior to slaughter. Plasma LH and FSH concentrations increased significantly (
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| doi_str_mv | 10.1016/S0378-4320(03)00159-3 |
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P<0.01) by 3 days after surgery, then fell gradually, but did not return to pre-surgery levels. Progesterone concentrations showed some evidence of cyclicity in all animals. In the grafted tissue, re-vascularisation of the tissue was apparent by 2 weeks post-grafting, although no preantral or antral follicles were observed. The tissue recovered after 24 weeks contained healthy preantral and antral follicles, luteal tissue and some large cystic follicles. It is unclear whether these cysts were the result of ovarian or hypothalamic/pituitary disturbance. In conclusion, the results of this study have shown that follicle growth and resumption of cyclicity can be achieved following ovarian autografting in pigs and indicate that this will be a useful model for investigating the mechanisms that control the early stages of follicular growth and ultimately ovulation rate in this multiovular species.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/S0378-4320(03)00159-3</identifier><identifier>PMID: 15036507</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Autografting ; Estrus - physiology ; Female ; Follicle Stimulating Hormone - blood ; Genitalia, Female - anatomy & histology ; Gilt ; Luteinizing Hormone - blood ; Organ Size ; Ovarian Follicle - physiology ; Ovary - blood supply ; Ovary - physiology ; Ovary - transplantation ; Pig-ovary ; Primordial follicles ; Progesterone - blood ; Re-vascularisation ; Swine ; Transplantation, Autologous</subject><ispartof>Animal reproduction science, 2004-02, Vol.80 (3), p.317-328</ispartof><rights>2003 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-8e870fd8119878b803378c4d701e33612c5b85b8e33aea4fca2bf768a8aee6593</citedby><cites>FETCH-LOGICAL-c385t-8e870fd8119878b803378c4d701e33612c5b85b8e33aea4fca2bf768a8aee6593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-4320(03)00159-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15036507$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hunter, M.G.</creatorcontrib><creatorcontrib>Hudson, N.</creatorcontrib><creatorcontrib>Mitchell, M.</creatorcontrib><creatorcontrib>Walker, R.M.</creatorcontrib><creatorcontrib>Webb, R.</creatorcontrib><title>Resumption of follicle growth in gilts after ovarian autografting</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The aims of the study were to evaluate autografting of porcine ovarian tissue in terms of establishment of a blood supply, follicle survival and development, commencement of oestrous cycles and endocrine patterns in this polyovular species. Experiment 1, a preliminary study on four gilts, showed that ovarian tissue slices survived the grafting procedure and re-vascularised. In Experiment 2, a further six pre-pubertal gilts had both ovaries surgically removed and two thin cortical slices of each ovary were immediately reattached to each of the ovarian pedicles. Blood samples were taken at surgery and then weekly. Two gilts were slaughtered 2 weeks after surgery and ovarian tissue recovered. The remaining four gilts underwent daily checks for behavioural oestrus until slaughter 24 weeks after surgery. All four gilts showed standing heat at least once prior to slaughter. Plasma LH and FSH concentrations increased significantly (
P<0.01) by 3 days after surgery, then fell gradually, but did not return to pre-surgery levels. Progesterone concentrations showed some evidence of cyclicity in all animals. In the grafted tissue, re-vascularisation of the tissue was apparent by 2 weeks post-grafting, although no preantral or antral follicles were observed. The tissue recovered after 24 weeks contained healthy preantral and antral follicles, luteal tissue and some large cystic follicles. It is unclear whether these cysts were the result of ovarian or hypothalamic/pituitary disturbance. In conclusion, the results of this study have shown that follicle growth and resumption of cyclicity can be achieved following ovarian autografting in pigs and indicate that this will be a useful model for investigating the mechanisms that control the early stages of follicular growth and ultimately ovulation rate in this multiovular species.</description><subject>Animals</subject><subject>Autografting</subject><subject>Estrus - physiology</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Genitalia, Female - anatomy & histology</subject><subject>Gilt</subject><subject>Luteinizing Hormone - blood</subject><subject>Organ Size</subject><subject>Ovarian Follicle - physiology</subject><subject>Ovary - blood supply</subject><subject>Ovary - physiology</subject><subject>Ovary - transplantation</subject><subject>Pig-ovary</subject><subject>Primordial follicles</subject><subject>Progesterone - blood</subject><subject>Re-vascularisation</subject><subject>Swine</subject><subject>Transplantation, Autologous</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQgIMouj5-gtqT6KE6abZJepJFfMGC4OMcsumkRrrNmrQr_nu720WPwsAwwzcPPkKOKVxSoPzqBZiQ6ZhlcA7sAoDmRcq2yIhKwdIsY9k2Gf0ie2Q_xg8AEJwXu2SP5sB4DmJEJs8Yu_midb5JvE2sr2tnakyq4L_a98Q1SeXqNibathgSv9TB6SbRXeur0PdcUx2SHavriEebfEDe7m5fbx7S6dP9481kmhom8zaVKAXYUlJaSCFnElj_nBmXAigyxmlm8pnsoy806rE1OptZwaWWGpHnBTsgZ8PeRfCfHcZWzV00WNe6Qd9FJagYU85ZD-YDaIKPMaBVi-DmOnwrCmrlTq3dqZUYBUyt3anV3MnmQDebY_k3tZHVA6cDYLVXugouqreXDCgDCqzIAXrieiCwF7F0GFQ0DhuDpQtoWlV6988TP_uNhw8</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Hunter, M.G.</creator><creator>Hudson, N.</creator><creator>Mitchell, M.</creator><creator>Walker, R.M.</creator><creator>Webb, R.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040201</creationdate><title>Resumption of follicle growth in gilts after ovarian autografting</title><author>Hunter, M.G. ; Hudson, N. ; Mitchell, M. ; Walker, R.M. ; Webb, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-8e870fd8119878b803378c4d701e33612c5b85b8e33aea4fca2bf768a8aee6593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Autografting</topic><topic>Estrus - physiology</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Genitalia, Female - anatomy & histology</topic><topic>Gilt</topic><topic>Luteinizing Hormone - blood</topic><topic>Organ Size</topic><topic>Ovarian Follicle - physiology</topic><topic>Ovary - blood supply</topic><topic>Ovary - physiology</topic><topic>Ovary - transplantation</topic><topic>Pig-ovary</topic><topic>Primordial follicles</topic><topic>Progesterone - blood</topic><topic>Re-vascularisation</topic><topic>Swine</topic><topic>Transplantation, Autologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hunter, M.G.</creatorcontrib><creatorcontrib>Hudson, N.</creatorcontrib><creatorcontrib>Mitchell, M.</creatorcontrib><creatorcontrib>Walker, R.M.</creatorcontrib><creatorcontrib>Webb, R.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hunter, M.G.</au><au>Hudson, N.</au><au>Mitchell, M.</au><au>Walker, R.M.</au><au>Webb, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resumption of follicle growth in gilts after ovarian autografting</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2004-02-01</date><risdate>2004</risdate><volume>80</volume><issue>3</issue><spage>317</spage><epage>328</epage><pages>317-328</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The aims of the study were to evaluate autografting of porcine ovarian tissue in terms of establishment of a blood supply, follicle survival and development, commencement of oestrous cycles and endocrine patterns in this polyovular species. Experiment 1, a preliminary study on four gilts, showed that ovarian tissue slices survived the grafting procedure and re-vascularised. In Experiment 2, a further six pre-pubertal gilts had both ovaries surgically removed and two thin cortical slices of each ovary were immediately reattached to each of the ovarian pedicles. Blood samples were taken at surgery and then weekly. Two gilts were slaughtered 2 weeks after surgery and ovarian tissue recovered. The remaining four gilts underwent daily checks for behavioural oestrus until slaughter 24 weeks after surgery. All four gilts showed standing heat at least once prior to slaughter. Plasma LH and FSH concentrations increased significantly (
P<0.01) by 3 days after surgery, then fell gradually, but did not return to pre-surgery levels. Progesterone concentrations showed some evidence of cyclicity in all animals. In the grafted tissue, re-vascularisation of the tissue was apparent by 2 weeks post-grafting, although no preantral or antral follicles were observed. The tissue recovered after 24 weeks contained healthy preantral and antral follicles, luteal tissue and some large cystic follicles. It is unclear whether these cysts were the result of ovarian or hypothalamic/pituitary disturbance. In conclusion, the results of this study have shown that follicle growth and resumption of cyclicity can be achieved following ovarian autografting in pigs and indicate that this will be a useful model for investigating the mechanisms that control the early stages of follicular growth and ultimately ovulation rate in this multiovular species.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15036507</pmid><doi>10.1016/S0378-4320(03)00159-3</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Autografting Estrus - physiology Female Follicle Stimulating Hormone - blood Genitalia, Female - anatomy & histology Gilt Luteinizing Hormone - blood Organ Size Ovarian Follicle - physiology Ovary - blood supply Ovary - physiology Ovary - transplantation Pig-ovary Primordial follicles Progesterone - blood Re-vascularisation Swine Transplantation, Autologous |
| title | Resumption of follicle growth in gilts after ovarian autografting |
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