Immunization with a DNA vaccine encoding the hepatitis-C-virus structural antigens elicits a specific immune response against the capsid and envelope proteins in rabbits and Macaca irus (crab-eating macaque monkeys)
In the present study, we evaluated the capability of the plasmid pIDKE2, encoding the HCV (hepatitis C virus) structural proteins Core, E1 and E2, to induce immune response against HCV antigens after injection into rabbits and Macaca irus (crab‐eating macaque). Animals were immunized intramuscularly...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2004-04, Vol.39 (2), p.249-255 |
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| creator | Dueñas-Carrera, Santiago Viña, Ariel Martínez, Rafael Alvarez-Lajonchere, Liz Alvarez-Obregón, Julio César Marante, Jeny Pérez, Anna Mosqueda, Omar Martínez, Gillian Morales, Juan |
| description | In the present study, we evaluated the capability of the plasmid pIDKE2, encoding the HCV (hepatitis C virus) structural proteins Core, E1 and E2, to induce immune response against HCV antigens after injection into rabbits and Macaca irus (crab‐eating macaque). Animals were immunized intramuscularly with different amounts of plasmid on weeks 0, 3 and 8. Monkeys received a booster dose on week 46. All rabbits immunized with pIDKE2 generated a positive antibody response and, particularly in rabbits immunized with 2 mg, antibody titres reached values above 1:1500 and 1:400 against the core and the envelope proteins, respectively, 28 weeks after primary immunization. The antibody response in monkeys developed slowly, but antibody titres greater than 1:3500 against HCV structural antigens were detected at week 52. Moreover, anti‐E2 antibodies recognized synthetic peptides covering the HVR‐1 (hypervariable region‐1) from different isolates corresponding to different genotypes. Additionally, a specific lymphoproliferative response against Core and E2 was detected in two out of three monkeys immunized with pIDKE2. The other monkey had a specific proliferative response to E1. Taking all these data together, immunization with pIDKE2 is able to elicit both humoral and cellular immunity against HCV structural antigens in animal models other than mice. |
| doi_str_mv | 10.1042/BA200301129 |
| format | Article |
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Animals were immunized intramuscularly with different amounts of plasmid on weeks 0, 3 and 8. Monkeys received a booster dose on week 46. All rabbits immunized with pIDKE2 generated a positive antibody response and, particularly in rabbits immunized with 2 mg, antibody titres reached values above 1:1500 and 1:400 against the core and the envelope proteins, respectively, 28 weeks after primary immunization. The antibody response in monkeys developed slowly, but antibody titres greater than 1:3500 against HCV structural antigens were detected at week 52. Moreover, anti‐E2 antibodies recognized synthetic peptides covering the HVR‐1 (hypervariable region‐1) from different isolates corresponding to different genotypes. Additionally, a specific lymphoproliferative response against Core and E2 was detected in two out of three monkeys immunized with pIDKE2. The other monkey had a specific proliferative response to E1. Taking all these data together, immunization with pIDKE2 is able to elicit both humoral and cellular immunity against HCV structural antigens in animal models other than mice.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1042/BA200301129</identifier><identifier>PMID: 15032746</identifier><identifier>CODEN: BABIEC</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; antibody response ; Antigens, Viral - administration & dosage ; Antigens, Viral - immunology ; Biological and medical sciences ; Biotechnology ; Capsid Proteins - immunology ; core ; envelope ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Transfer Techniques ; Genetic Therapy - methods ; Hepatitis C - immunology ; Hepatitis C - prevention & control ; Hepatitis C Antibodies - immunology ; Hepatitis C virus ; HVR-1 ; Immunization - methods ; Immunotherapy - methods ; lymphoproliferation ; Macaca ; Macaca irus ; Male ; Rabbits ; Species Specificity ; Vaccination - methods ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology ; Viral Envelope Proteins - metabolism</subject><ispartof>Biotechnology and applied biochemistry, 2004-04, Vol.39 (2), p.249-255</ispartof><rights>2004 International Union of Biochemistry and Molecular Biology</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4270-1ad7ac069a19f1948abb6863ae73bbc678757a37be3f4f5b6f50df3c915b244e3</citedby><cites>FETCH-LOGICAL-c4270-1ad7ac069a19f1948abb6863ae73bbc678757a37be3f4f5b6f50df3c915b244e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1042%2FBA200301129$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1042%2FBA200301129$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15660299$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15032746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dueñas-Carrera, Santiago</creatorcontrib><creatorcontrib>Viña, Ariel</creatorcontrib><creatorcontrib>Martínez, Rafael</creatorcontrib><creatorcontrib>Alvarez-Lajonchere, Liz</creatorcontrib><creatorcontrib>Alvarez-Obregón, Julio César</creatorcontrib><creatorcontrib>Marante, Jeny</creatorcontrib><creatorcontrib>Pérez, Anna</creatorcontrib><creatorcontrib>Mosqueda, Omar</creatorcontrib><creatorcontrib>Martínez, Gillian</creatorcontrib><creatorcontrib>Morales, Juan</creatorcontrib><title>Immunization with a DNA vaccine encoding the hepatitis-C-virus structural antigens elicits a specific immune response against the capsid and envelope proteins in rabbits and Macaca irus (crab-eating macaque monkeys)</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnol Appl Biochem</addtitle><description>In the present study, we evaluated the capability of the plasmid pIDKE2, encoding the HCV (hepatitis C virus) structural proteins Core, E1 and E2, to induce immune response against HCV antigens after injection into rabbits and Macaca irus (crab‐eating macaque). Animals were immunized intramuscularly with different amounts of plasmid on weeks 0, 3 and 8. Monkeys received a booster dose on week 46. All rabbits immunized with pIDKE2 generated a positive antibody response and, particularly in rabbits immunized with 2 mg, antibody titres reached values above 1:1500 and 1:400 against the core and the envelope proteins, respectively, 28 weeks after primary immunization. The antibody response in monkeys developed slowly, but antibody titres greater than 1:3500 against HCV structural antigens were detected at week 52. Moreover, anti‐E2 antibodies recognized synthetic peptides covering the HVR‐1 (hypervariable region‐1) from different isolates corresponding to different genotypes. Additionally, a specific lymphoproliferative response against Core and E2 was detected in two out of three monkeys immunized with pIDKE2. The other monkey had a specific proliferative response to E1. Taking all these data together, immunization with pIDKE2 is able to elicit both humoral and cellular immunity against HCV structural antigens in animal models other than mice.</description><subject>Animals</subject><subject>antibody response</subject><subject>Antigens, Viral - administration & dosage</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Capsid Proteins - immunology</subject><subject>core</subject><subject>envelope</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Therapy - methods</subject><subject>Hepatitis C - immunology</subject><subject>Hepatitis C - prevention & control</subject><subject>Hepatitis C Antibodies - immunology</subject><subject>Hepatitis C virus</subject><subject>HVR-1</subject><subject>Immunization - methods</subject><subject>Immunotherapy - methods</subject><subject>lymphoproliferation</subject><subject>Macaca</subject><subject>Macaca irus</subject><subject>Male</subject><subject>Rabbits</subject><subject>Species Specificity</subject><subject>Vaccination - methods</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><subject>Viral Envelope Proteins - metabolism</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhSMEoqWwYo-8AYFQwI6TOFlOByiVSmExaJbRjXMzY5o4wdeZMrwor4PnR9AVyAtb9nfOufKJoqeCvxE8Td6ezxLOJRciKe9FpyJVPC5Umt6PTnlRZHGaCXkSPSL6xjkvVJE8jE5ExmWi0vw0-nXZ95M1P8GbwbJb49cM2LvrGduA1sYiQ6uHxtgV82tkaxwD6A3F83hj3ESMvJu0nxx0DKw3K7TEsDPaeApGNKI2rdHM7FKQOaRxsIQMVmAs-b2phpFME-RNCNtgN4zIRjd4DAQzljmo671dAD6BDovto1_q8BJjGChM14fr7xOyfrA3uKVXj6MHLXSET477WfT1w_vF_GN89fnicj67inWahI8S0CjQPC9BlK0o0yJk5UUuAZWsa52rQmUKpKpRtmmb1Xmb8aaVuhRZnaQpyrPoxcE3TBzyyVe9IY1dBxaHiSollCxDD_8FhSpzUSQ8gK8PoHYDkcO2Gp3pwW0rwatd4dWdwgP97Gg71T02f9ljwwF4fgSANHStA6sN3eHynCflzogfuFvT4fZfmeF8LsLnBUl8kBjy-OOPBNxNlSupsmp5fVEtlvPlF1EuqkL-BoTR1Dc</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Dueñas-Carrera, Santiago</creator><creator>Viña, Ariel</creator><creator>Martínez, Rafael</creator><creator>Alvarez-Lajonchere, Liz</creator><creator>Alvarez-Obregón, Julio César</creator><creator>Marante, Jeny</creator><creator>Pérez, Anna</creator><creator>Mosqueda, Omar</creator><creator>Martínez, Gillian</creator><creator>Morales, Juan</creator><general>Blackwell Publishing Ltd</general><general>Portland Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200404</creationdate><title>Immunization with a DNA vaccine encoding the hepatitis-C-virus structural antigens elicits a specific immune response against the capsid and envelope proteins in rabbits and Macaca irus (crab-eating macaque monkeys)</title><author>Dueñas-Carrera, Santiago ; Viña, Ariel ; Martínez, Rafael ; Alvarez-Lajonchere, Liz ; Alvarez-Obregón, Julio César ; Marante, Jeny ; Pérez, Anna ; Mosqueda, Omar ; Martínez, Gillian ; Morales, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4270-1ad7ac069a19f1948abb6863ae73bbc678757a37be3f4f5b6f50df3c915b244e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>antibody response</topic><topic>Antigens, Viral - administration & dosage</topic><topic>Antigens, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Capsid Proteins - immunology</topic><topic>core</topic><topic>envelope</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Therapy - methods</topic><topic>Hepatitis C - immunology</topic><topic>Hepatitis C - prevention & control</topic><topic>Hepatitis C Antibodies - immunology</topic><topic>Hepatitis C virus</topic><topic>HVR-1</topic><topic>Immunization - methods</topic><topic>Immunotherapy - methods</topic><topic>lymphoproliferation</topic><topic>Macaca</topic><topic>Macaca irus</topic><topic>Male</topic><topic>Rabbits</topic><topic>Species Specificity</topic><topic>Vaccination - methods</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><topic>Viral Envelope Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dueñas-Carrera, Santiago</creatorcontrib><creatorcontrib>Viña, Ariel</creatorcontrib><creatorcontrib>Martínez, Rafael</creatorcontrib><creatorcontrib>Alvarez-Lajonchere, Liz</creatorcontrib><creatorcontrib>Alvarez-Obregón, Julio César</creatorcontrib><creatorcontrib>Marante, Jeny</creatorcontrib><creatorcontrib>Pérez, Anna</creatorcontrib><creatorcontrib>Mosqueda, Omar</creatorcontrib><creatorcontrib>Martínez, Gillian</creatorcontrib><creatorcontrib>Morales, Juan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dueñas-Carrera, Santiago</au><au>Viña, Ariel</au><au>Martínez, Rafael</au><au>Alvarez-Lajonchere, Liz</au><au>Alvarez-Obregón, Julio César</au><au>Marante, Jeny</au><au>Pérez, Anna</au><au>Mosqueda, Omar</au><au>Martínez, Gillian</au><au>Morales, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunization with a DNA vaccine encoding the hepatitis-C-virus structural antigens elicits a specific immune response against the capsid and envelope proteins in rabbits and Macaca irus (crab-eating macaque monkeys)</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><addtitle>Biotechnol Appl Biochem</addtitle><date>2004-04</date><risdate>2004</risdate><volume>39</volume><issue>2</issue><spage>249</spage><epage>255</epage><pages>249-255</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><coden>BABIEC</coden><abstract>In the present study, we evaluated the capability of the plasmid pIDKE2, encoding the HCV (hepatitis C virus) structural proteins Core, E1 and E2, to induce immune response against HCV antigens after injection into rabbits and Macaca irus (crab‐eating macaque). Animals were immunized intramuscularly with different amounts of plasmid on weeks 0, 3 and 8. Monkeys received a booster dose on week 46. All rabbits immunized with pIDKE2 generated a positive antibody response and, particularly in rabbits immunized with 2 mg, antibody titres reached values above 1:1500 and 1:400 against the core and the envelope proteins, respectively, 28 weeks after primary immunization. The antibody response in monkeys developed slowly, but antibody titres greater than 1:3500 against HCV structural antigens were detected at week 52. Moreover, anti‐E2 antibodies recognized synthetic peptides covering the HVR‐1 (hypervariable region‐1) from different isolates corresponding to different genotypes. Additionally, a specific lymphoproliferative response against Core and E2 was detected in two out of three monkeys immunized with pIDKE2. The other monkey had a specific proliferative response to E1. Taking all these data together, immunization with pIDKE2 is able to elicit both humoral and cellular immunity against HCV structural antigens in animal models other than mice.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15032746</pmid><doi>10.1042/BA200301129</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals antibody response Antigens, Viral - administration & dosage Antigens, Viral - immunology Biological and medical sciences Biotechnology Capsid Proteins - immunology core envelope Female Fundamental and applied biological sciences. Psychology Gene Transfer Techniques Genetic Therapy - methods Hepatitis C - immunology Hepatitis C - prevention & control Hepatitis C Antibodies - immunology Hepatitis C virus HVR-1 Immunization - methods Immunotherapy - methods lymphoproliferation Macaca Macaca irus Male Rabbits Species Specificity Vaccination - methods Vaccines, DNA - administration & dosage Vaccines, DNA - immunology Viral Envelope Proteins - metabolism |
| title | Immunization with a DNA vaccine encoding the hepatitis-C-virus structural antigens elicits a specific immune response against the capsid and envelope proteins in rabbits and Macaca irus (crab-eating macaque monkeys) |
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