Photochemical immobilization of proteins on microwave–synthesized photoreactive polymers

We report a rapid and versatile procedure for the preparation of photoreactive polymers and light-induced immobilization of proteins onto such polymers. Photoreactive controlled-pore glass, silica gel, glass slide, and polystyrene microtiter plate are prepared in 40–60 s by microwave irradiation of...

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Veröffentlicht in:	Analytical biochemistry 2004-04, Vol.327 (1), p.68-73
Hauptverfasser:	Naqvi, Azmi, Nahar, Pradip
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Fluoro-2-nitro-4-azidobenzene Azides - chemistry Enzymes, Immobilized - chemistry Glass - chemistry Glucose Oxidase - chemistry Glucose Oxidase - radiation effects Horseradish peroxidase Horseradish Peroxidase - chemistry Horseradish Peroxidase - radiation effects Microwave Microwaves Nitrobenzenes - chemistry Photochemistry - methods Photoimmobilization Photoreactive polymer Polymers - chemical synthesis Polymers - radiation effects Silica Gel Silicon Dioxide - chemistry Ultraviolet Rays
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