Characteristics of Lysophosphatidylcholine in Its Inhibition of Taurine Uptake by Human Intestinal Caco-2 Cells
The characteristics of lysophosphatidylcholine (LPC) in its inhibition of the taurine uptake by human intestinal Caco-2 cells were investigated. By treating the cells with 200 μM of LPC, the taurine uptake was rapidly decreased by approximately 60%. This decrease was accompanied by an increase in th...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002, Vol.66 (4), p.730-736 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | ISHIZUKA, Koji MIYAMOTO, Yusei SATSU, Hideo SATO, Ryuichiro SHIMIZU, Makoto |
| description | The characteristics of lysophosphatidylcholine (LPC) in its inhibition of the taurine uptake by human intestinal Caco-2 cells were investigated. By treating the cells with 200 μM of LPC, the taurine uptake was rapidly decreased by approximately 60%. This decrease was accompanied by an increase in the K
m
value for the uptake. A rapid uptake of LPC itself by the cells was also observed. The inhibitory activity of LPC was specific to the uptake of taurine and certain amino acids, while the uptake of glucose, glutamic acid and peptide (glycylglutamine) was not affected by LPC. The activity was dependent on the structure of a polar head and the bound fatty acid. The phosphorylcholine residue was likely to have played an important role, and surface active LPC with fatty acids of C14 or longer was highly inhibitory. These results suggest that the interaction of LPC with the taurine transporter in the intestinal cell membrane was the cause of the reduced taurine uptake. |
| doi_str_mv | 10.1271/bbb.66.730 |
| format | Article |
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m
value for the uptake. A rapid uptake of LPC itself by the cells was also observed. The inhibitory activity of LPC was specific to the uptake of taurine and certain amino acids, while the uptake of glucose, glutamic acid and peptide (glycylglutamine) was not affected by LPC. The activity was dependent on the structure of a polar head and the bound fatty acid. The phosphorylcholine residue was likely to have played an important role, and surface active LPC with fatty acids of C14 or longer was highly inhibitory. These results suggest that the interaction of LPC with the taurine transporter in the intestinal cell membrane was the cause of the reduced taurine uptake.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.66.730</identifier><identifier>PMID: 12036043</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Biological and medical sciences ; Biological Transport - drug effects ; Caco-2 ; Caco-2 Cells ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Humans ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Intestine. Mesentery ; Kinetics ; lysophosphatidylcholine ; Lysophosphatidylcholines - pharmacology ; small intestine ; Surface Tension ; taurine ; Taurine - antagonists & inhibitors ; Taurine - metabolism ; Taurocholic Acid - pharmacology ; transporter ; Vertebrates: digestive system</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2002, Vol.66 (4), p.730-736</ispartof><rights>2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2002</rights><rights>2002 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c603t-2329668c92d5ed29f2ef91b91798d00a89bac636595ac1a06736953b99b5c34d3</citedby><cites>FETCH-LOGICAL-c603t-2329668c92d5ed29f2ef91b91798d00a89bac636595ac1a06736953b99b5c34d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13696547$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12036043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ISHIZUKA, Koji</creatorcontrib><creatorcontrib>MIYAMOTO, Yusei</creatorcontrib><creatorcontrib>SATSU, Hideo</creatorcontrib><creatorcontrib>SATO, Ryuichiro</creatorcontrib><creatorcontrib>SHIMIZU, Makoto</creatorcontrib><title>Characteristics of Lysophosphatidylcholine in Its Inhibition of Taurine Uptake by Human Intestinal Caco-2 Cells</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The characteristics of lysophosphatidylcholine (LPC) in its inhibition of the taurine uptake by human intestinal Caco-2 cells were investigated. By treating the cells with 200 μM of LPC, the taurine uptake was rapidly decreased by approximately 60%. This decrease was accompanied by an increase in the K
m
value for the uptake. A rapid uptake of LPC itself by the cells was also observed. The inhibitory activity of LPC was specific to the uptake of taurine and certain amino acids, while the uptake of glucose, glutamic acid and peptide (glycylglutamine) was not affected by LPC. The activity was dependent on the structure of a polar head and the bound fatty acid. The phosphorylcholine residue was likely to have played an important role, and surface active LPC with fatty acids of C14 or longer was highly inhibitory. These results suggest that the interaction of LPC with the taurine transporter in the intestinal cell membrane was the cause of the reduced taurine uptake.</description><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Caco-2</subject><subject>Caco-2 Cells</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestine. Mesentery</subject><subject>Kinetics</subject><subject>lysophosphatidylcholine</subject><subject>Lysophosphatidylcholines - pharmacology</subject><subject>small intestine</subject><subject>Surface Tension</subject><subject>taurine</subject><subject>Taurine - antagonists & inhibitors</subject><subject>Taurine - metabolism</subject><subject>Taurocholic Acid - pharmacology</subject><subject>transporter</subject><subject>Vertebrates: digestive system</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0U2L1DAYB_AgijuuXvwAUhA9CB2T5qWToxR1Bwa87J7DkzSlWdOkJi3Sb2-GGVkQDyGQ_PLknzwIvSV4T5qWfNZa74XYtxQ_QztCWVsLydrnaIclEfWBcXKDXuX8iHFZ4OQluiENpgIzukOxGyGBWWxyeXEmV3GoTluO8xjzPMLi-s2bMXoXbOVCdVxydQyj025xMZzxPazpvPkwL_DTVnqr7tYJigyLLRUD-KoDE-um6qz3-TV6MYDP9s11vkUP377ed3f16cf3Y_flVBuB6VI3tJFCHIxsem77Rg6NHSTRkrTy0GMMB6nBCCq45GAIYNFSITnVUmpuKOvpLfp4qTun-GstSdTksikJINi4ZtWSlpbBCnz_D3yMayqxsyKMScY4a3FRny7KpJhzsoOak5sgbYpgdW6CKk1QQqjShILfXUuuerL9E73-egEfrgCyAT8kCMblJ1feIsq1xfGLc2GIaYLfMfleLbD5mP4eov8J8AdXcKDI</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>ISHIZUKA, Koji</creator><creator>MIYAMOTO, Yusei</creator><creator>SATSU, Hideo</creator><creator>SATO, Ryuichiro</creator><creator>SHIMIZU, Makoto</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Characteristics of Lysophosphatidylcholine in Its Inhibition of Taurine Uptake by Human Intestinal Caco-2 Cells</title><author>ISHIZUKA, Koji ; MIYAMOTO, Yusei ; SATSU, Hideo ; SATO, Ryuichiro ; SHIMIZU, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c603t-2329668c92d5ed29f2ef91b91798d00a89bac636595ac1a06736953b99b5c34d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Caco-2</topic><topic>Caco-2 Cells</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestine. Mesentery</topic><topic>Kinetics</topic><topic>lysophosphatidylcholine</topic><topic>Lysophosphatidylcholines - pharmacology</topic><topic>small intestine</topic><topic>Surface Tension</topic><topic>taurine</topic><topic>Taurine - antagonists & inhibitors</topic><topic>Taurine - metabolism</topic><topic>Taurocholic Acid - pharmacology</topic><topic>transporter</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ISHIZUKA, Koji</creatorcontrib><creatorcontrib>MIYAMOTO, Yusei</creatorcontrib><creatorcontrib>SATSU, Hideo</creatorcontrib><creatorcontrib>SATO, Ryuichiro</creatorcontrib><creatorcontrib>SHIMIZU, Makoto</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ISHIZUKA, Koji</au><au>MIYAMOTO, Yusei</au><au>SATSU, Hideo</au><au>SATO, Ryuichiro</au><au>SHIMIZU, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characteristics of Lysophosphatidylcholine in Its Inhibition of Taurine Uptake by Human Intestinal Caco-2 Cells</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2002</date><risdate>2002</risdate><volume>66</volume><issue>4</issue><spage>730</spage><epage>736</epage><pages>730-736</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>The characteristics of lysophosphatidylcholine (LPC) in its inhibition of the taurine uptake by human intestinal Caco-2 cells were investigated. By treating the cells with 200 μM of LPC, the taurine uptake was rapidly decreased by approximately 60%. This decrease was accompanied by an increase in the K
m
value for the uptake. A rapid uptake of LPC itself by the cells was also observed. The inhibitory activity of LPC was specific to the uptake of taurine and certain amino acids, while the uptake of glucose, glutamic acid and peptide (glycylglutamine) was not affected by LPC. The activity was dependent on the structure of a polar head and the bound fatty acid. The phosphorylcholine residue was likely to have played an important role, and surface active LPC with fatty acids of C14 or longer was highly inhibitory. These results suggest that the interaction of LPC with the taurine transporter in the intestinal cell membrane was the cause of the reduced taurine uptake.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>12036043</pmid><doi>10.1271/bbb.66.730</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | Biological and medical sciences Biological Transport - drug effects Caco-2 Caco-2 Cells Culture Media Fundamental and applied biological sciences. Psychology Humans Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Intestine. Mesentery Kinetics lysophosphatidylcholine Lysophosphatidylcholines - pharmacology small intestine Surface Tension taurine Taurine - antagonists & inhibitors Taurine - metabolism Taurocholic Acid - pharmacology transporter Vertebrates: digestive system |
| title | Characteristics of Lysophosphatidylcholine in Its Inhibition of Taurine Uptake by Human Intestinal Caco-2 Cells |
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