A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures
Background & Aims : Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract. Methods : One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary trac...
Gespeichert in:
| Veröffentlicht in: | Clinical gastroenterology and hepatology 2004-03, Vol.2 (3), p.214-219 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 219 |
|---|---|
| container_issue | 3 |
| container_start_page | 214 |
| container_title | Clinical gastroenterology and hepatology |
| container_volume | 2 |
| creator | Baron, Todd H Harewood, Gavin C Rumalla, Ashwin Pochron, Nicole L Stadheim, Linda M Gores, Gregory J Therneau, Terry M De Groen, Piet C Sebo, Thomas J Salomao, Diva R Kipp, Benjamin R |
| description | Background & Aims
: Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract.
Methods
: One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary tract strictures were enrolled in a prospective study comparing the accuracy of DIA and routine cytology (RC). Standard brush cytology sampling was performed twice by using 2 cytology brushes per patient. Both brushes were fixed in a single-specimen vial. Each specimen was formed into 1 pellet, and the sample was equally divided for evaluation by DIA and RC. DNA histograms were generated for ploidy analysis. The DIA criterion for malignancy was demonstration of aneuploidy.
Results
: Two patients had inadequate samples obtained for DIA analysis, 7 benign patients were excluded because of inadequate follow-up of less than 75 days, and 1 patient was lost to follow-up to clarify malignant versus benign disease. Of the remaining 100 patients, 56 strictures were malignant and 44 were benign. The sensitivities of DIA and RC were 39.3% and 17.9%, respectively (
P = 0.014). The specificities of DIA and RC were 77.3% and 97.7%, respectively (
P = 0.003). The accuracy of DIA (56.0%) was equivalent to RC (53.0%).
Conclusions
: DIA is a valuable adjunct to RC for detecting malignant strictures of the biliary tract. |
| doi_str_mv | 10.1016/S1542-3565(04)00006-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71735577</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1542356504000060</els_id><sourcerecordid>71735577</sourcerecordid><originalsourceid>FETCH-LOGICAL-c363t-ec0df3a44cbb41e878806c5c88598984e4cf4f1a24b68632d54edea910aad5c83</originalsourceid><addsrcrecordid>eNqFkc1q3TAQhUVpyV_zCA1alXThRrqWLHsVQkjTQqCLpGshS-PbKbZ0I8kBv0EeO7o_kGW10TB8Z4Y5h5AvnH3njDdXj1yKVVXLRl4y8Y2V11TsAznZtZXi4uOh3iLH5DSlf4ytOtGpI3LMJeOqYfKEvN7QTQxpAzbjC1Abpo2JmIKnYaAO15jNSHEya6DGm3FJmErhaAxzRl8ESw5jWC90CJHmv0DRgc84oDUZ91MmM-LaG28Xip72OKKJC83R2ExTjmjzHCF9Jp8GMyY4P_xn5M-Pu6fbn9XD7_tftzcPla2bOldgmRtqI4Tte8GhVW3LGitt28qu7VoBwg5i4GYl-qZt6pWTAhyYjjNjXMHqM_J1P7ec_TxDynrCZGEcjYcwJ624qqVUqoByD9riT4ow6E0sRsRFc6a3EehdBHrrr2ZC7yLQrOguDgvmfgL3rjp4XoDrPQDlzBeEqJNF8BYcxhKDdgH_s-IN4xWZnA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71735577</pqid></control><display><type>article</type><title>A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Baron, Todd H ; Harewood, Gavin C ; Rumalla, Ashwin ; Pochron, Nicole L ; Stadheim, Linda M ; Gores, Gregory J ; Therneau, Terry M ; De Groen, Piet C ; Sebo, Thomas J ; Salomao, Diva R ; Kipp, Benjamin R</creator><creatorcontrib>Baron, Todd H ; Harewood, Gavin C ; Rumalla, Ashwin ; Pochron, Nicole L ; Stadheim, Linda M ; Gores, Gregory J ; Therneau, Terry M ; De Groen, Piet C ; Sebo, Thomas J ; Salomao, Diva R ; Kipp, Benjamin R</creatorcontrib><description>Background & Aims
: Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract.
Methods
: One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary tract strictures were enrolled in a prospective study comparing the accuracy of DIA and routine cytology (RC). Standard brush cytology sampling was performed twice by using 2 cytology brushes per patient. Both brushes were fixed in a single-specimen vial. Each specimen was formed into 1 pellet, and the sample was equally divided for evaluation by DIA and RC. DNA histograms were generated for ploidy analysis. The DIA criterion for malignancy was demonstration of aneuploidy.
Results
: Two patients had inadequate samples obtained for DIA analysis, 7 benign patients were excluded because of inadequate follow-up of less than 75 days, and 1 patient was lost to follow-up to clarify malignant versus benign disease. Of the remaining 100 patients, 56 strictures were malignant and 44 were benign. The sensitivities of DIA and RC were 39.3% and 17.9%, respectively (
P = 0.014). The specificities of DIA and RC were 77.3% and 97.7%, respectively (
P = 0.003). The accuracy of DIA (56.0%) was equivalent to RC (53.0%).
Conclusions
: DIA is a valuable adjunct to RC for detecting malignant strictures of the biliary tract.</description><identifier>ISSN: 1542-3565</identifier><identifier>EISSN: 1542-7714</identifier><identifier>DOI: 10.1016/S1542-3565(04)00006-0</identifier><identifier>PMID: 15017605</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Aged ; Biliary Tract Diseases - pathology ; Biliary Tract Neoplasms - pathology ; Biopsy, Needle ; Cholangiopancreatography, Endoscopic Retrograde - methods ; Cytodiagnosis ; Diagnosis, Differential ; Diagnostic Imaging - methods ; DNA, Neoplasm - analysis ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Prospective Studies ; Sensitivity and Specificity ; Signal Processing, Computer-Assisted</subject><ispartof>Clinical gastroenterology and hepatology, 2004-03, Vol.2 (3), p.214-219</ispartof><rights>2004 American Gastroenterological Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-ec0df3a44cbb41e878806c5c88598984e4cf4f1a24b68632d54edea910aad5c83</citedby><cites>FETCH-LOGICAL-c363t-ec0df3a44cbb41e878806c5c88598984e4cf4f1a24b68632d54edea910aad5c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1542-3565(04)00006-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15017605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baron, Todd H</creatorcontrib><creatorcontrib>Harewood, Gavin C</creatorcontrib><creatorcontrib>Rumalla, Ashwin</creatorcontrib><creatorcontrib>Pochron, Nicole L</creatorcontrib><creatorcontrib>Stadheim, Linda M</creatorcontrib><creatorcontrib>Gores, Gregory J</creatorcontrib><creatorcontrib>Therneau, Terry M</creatorcontrib><creatorcontrib>De Groen, Piet C</creatorcontrib><creatorcontrib>Sebo, Thomas J</creatorcontrib><creatorcontrib>Salomao, Diva R</creatorcontrib><creatorcontrib>Kipp, Benjamin R</creatorcontrib><title>A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures</title><title>Clinical gastroenterology and hepatology</title><addtitle>Clin Gastroenterol Hepatol</addtitle><description>Background & Aims
: Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract.
Methods
: One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary tract strictures were enrolled in a prospective study comparing the accuracy of DIA and routine cytology (RC). Standard brush cytology sampling was performed twice by using 2 cytology brushes per patient. Both brushes were fixed in a single-specimen vial. Each specimen was formed into 1 pellet, and the sample was equally divided for evaluation by DIA and RC. DNA histograms were generated for ploidy analysis. The DIA criterion for malignancy was demonstration of aneuploidy.
Results
: Two patients had inadequate samples obtained for DIA analysis, 7 benign patients were excluded because of inadequate follow-up of less than 75 days, and 1 patient was lost to follow-up to clarify malignant versus benign disease. Of the remaining 100 patients, 56 strictures were malignant and 44 were benign. The sensitivities of DIA and RC were 39.3% and 17.9%, respectively (
P = 0.014). The specificities of DIA and RC were 77.3% and 97.7%, respectively (
P = 0.003). The accuracy of DIA (56.0%) was equivalent to RC (53.0%).
Conclusions
: DIA is a valuable adjunct to RC for detecting malignant strictures of the biliary tract.</description><subject>Adult</subject><subject>Aged</subject><subject>Biliary Tract Diseases - pathology</subject><subject>Biliary Tract Neoplasms - pathology</subject><subject>Biopsy, Needle</subject><subject>Cholangiopancreatography, Endoscopic Retrograde - methods</subject><subject>Cytodiagnosis</subject><subject>Diagnosis, Differential</subject><subject>Diagnostic Imaging - methods</subject><subject>DNA, Neoplasm - analysis</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Prospective Studies</subject><subject>Sensitivity and Specificity</subject><subject>Signal Processing, Computer-Assisted</subject><issn>1542-3565</issn><issn>1542-7714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1q3TAQhUVpyV_zCA1alXThRrqWLHsVQkjTQqCLpGshS-PbKbZ0I8kBv0EeO7o_kGW10TB8Z4Y5h5AvnH3njDdXj1yKVVXLRl4y8Y2V11TsAznZtZXi4uOh3iLH5DSlf4ytOtGpI3LMJeOqYfKEvN7QTQxpAzbjC1Abpo2JmIKnYaAO15jNSHEya6DGm3FJmErhaAxzRl8ESw5jWC90CJHmv0DRgc84oDUZ91MmM-LaG28Xip72OKKJC83R2ExTjmjzHCF9Jp8GMyY4P_xn5M-Pu6fbn9XD7_tftzcPla2bOldgmRtqI4Tte8GhVW3LGitt28qu7VoBwg5i4GYl-qZt6pWTAhyYjjNjXMHqM_J1P7ec_TxDynrCZGEcjYcwJ624qqVUqoByD9riT4ow6E0sRsRFc6a3EehdBHrrr2ZC7yLQrOguDgvmfgL3rjp4XoDrPQDlzBeEqJNF8BYcxhKDdgH_s-IN4xWZnA</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Baron, Todd H</creator><creator>Harewood, Gavin C</creator><creator>Rumalla, Ashwin</creator><creator>Pochron, Nicole L</creator><creator>Stadheim, Linda M</creator><creator>Gores, Gregory J</creator><creator>Therneau, Terry M</creator><creator>De Groen, Piet C</creator><creator>Sebo, Thomas J</creator><creator>Salomao, Diva R</creator><creator>Kipp, Benjamin R</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040301</creationdate><title>A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures</title><author>Baron, Todd H ; Harewood, Gavin C ; Rumalla, Ashwin ; Pochron, Nicole L ; Stadheim, Linda M ; Gores, Gregory J ; Therneau, Terry M ; De Groen, Piet C ; Sebo, Thomas J ; Salomao, Diva R ; Kipp, Benjamin R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-ec0df3a44cbb41e878806c5c88598984e4cf4f1a24b68632d54edea910aad5c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biliary Tract Diseases - pathology</topic><topic>Biliary Tract Neoplasms - pathology</topic><topic>Biopsy, Needle</topic><topic>Cholangiopancreatography, Endoscopic Retrograde - methods</topic><topic>Cytodiagnosis</topic><topic>Diagnosis, Differential</topic><topic>Diagnostic Imaging - methods</topic><topic>DNA, Neoplasm - analysis</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Prospective Studies</topic><topic>Sensitivity and Specificity</topic><topic>Signal Processing, Computer-Assisted</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baron, Todd H</creatorcontrib><creatorcontrib>Harewood, Gavin C</creatorcontrib><creatorcontrib>Rumalla, Ashwin</creatorcontrib><creatorcontrib>Pochron, Nicole L</creatorcontrib><creatorcontrib>Stadheim, Linda M</creatorcontrib><creatorcontrib>Gores, Gregory J</creatorcontrib><creatorcontrib>Therneau, Terry M</creatorcontrib><creatorcontrib>De Groen, Piet C</creatorcontrib><creatorcontrib>Sebo, Thomas J</creatorcontrib><creatorcontrib>Salomao, Diva R</creatorcontrib><creatorcontrib>Kipp, Benjamin R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical gastroenterology and hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baron, Todd H</au><au>Harewood, Gavin C</au><au>Rumalla, Ashwin</au><au>Pochron, Nicole L</au><au>Stadheim, Linda M</au><au>Gores, Gregory J</au><au>Therneau, Terry M</au><au>De Groen, Piet C</au><au>Sebo, Thomas J</au><au>Salomao, Diva R</au><au>Kipp, Benjamin R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures</atitle><jtitle>Clinical gastroenterology and hepatology</jtitle><addtitle>Clin Gastroenterol Hepatol</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>2</volume><issue>3</issue><spage>214</spage><epage>219</epage><pages>214-219</pages><issn>1542-3565</issn><eissn>1542-7714</eissn><abstract>Background & Aims
: Digital image analysis (DIA) allows quantification of nuclear DNA content and may help distinguish benign and malignant strictures of the biliary tract.
Methods
: One hundred ten consecutive patients undergoing endoscopic retrograde cholangiography for suspicious biliary tract strictures were enrolled in a prospective study comparing the accuracy of DIA and routine cytology (RC). Standard brush cytology sampling was performed twice by using 2 cytology brushes per patient. Both brushes were fixed in a single-specimen vial. Each specimen was formed into 1 pellet, and the sample was equally divided for evaluation by DIA and RC. DNA histograms were generated for ploidy analysis. The DIA criterion for malignancy was demonstration of aneuploidy.
Results
: Two patients had inadequate samples obtained for DIA analysis, 7 benign patients were excluded because of inadequate follow-up of less than 75 days, and 1 patient was lost to follow-up to clarify malignant versus benign disease. Of the remaining 100 patients, 56 strictures were malignant and 44 were benign. The sensitivities of DIA and RC were 39.3% and 17.9%, respectively (
P = 0.014). The specificities of DIA and RC were 77.3% and 97.7%, respectively (
P = 0.003). The accuracy of DIA (56.0%) was equivalent to RC (53.0%).
Conclusions
: DIA is a valuable adjunct to RC for detecting malignant strictures of the biliary tract.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15017605</pmid><doi>10.1016/S1542-3565(04)00006-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1542-3565 |
| ispartof | Clinical gastroenterology and hepatology, 2004-03, Vol.2 (3), p.214-219 |
| issn | 1542-3565 1542-7714 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71735577 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Aged Biliary Tract Diseases - pathology Biliary Tract Neoplasms - pathology Biopsy, Needle Cholangiopancreatography, Endoscopic Retrograde - methods Cytodiagnosis Diagnosis, Differential Diagnostic Imaging - methods DNA, Neoplasm - analysis Female Humans Immunohistochemistry Male Middle Aged Prospective Studies Sensitivity and Specificity Signal Processing, Computer-Assisted |
| title | A prospective comparison of digital image analysis and routine cytology for the identification of malignancy in biliary tract strictures |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T14%3A08%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20prospective%20comparison%20of%20digital%20image%20analysis%20and%20routine%20cytology%20for%20the%20identification%20of%20malignancy%20in%20biliary%20tract%20strictures&rft.jtitle=Clinical%20gastroenterology%20and%20hepatology&rft.au=Baron,%20Todd%20H&rft.date=2004-03-01&rft.volume=2&rft.issue=3&rft.spage=214&rft.epage=219&rft.pages=214-219&rft.issn=1542-3565&rft.eissn=1542-7714&rft_id=info:doi/10.1016/S1542-3565(04)00006-0&rft_dat=%3Cproquest_cross%3E71735577%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71735577&rft_id=info:pmid/15017605&rft_els_id=S1542356504000060&rfr_iscdi=true |