Human Immunoglobulin G Mediates Protective Immunity and Identifies Protective Antigens against Larval Strongyloides stercoralis in Mice
Protective immunity to larval Strongyloides stercoralis in mice has been shown to be dependent on antibody, complement, and granulocytes. The goals of the present study was to determine the following: (1) whether human serum could passively transfer immunity to mice, (2) the mechanism by which the s...
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| Veröffentlicht in: | The Journal of infectious diseases 2004-04, Vol.189 (7), p.1282-1290 |
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| container_title | The Journal of infectious diseases |
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| creator | Kerepesi, Laura A. Nolan, Thomas J. Schad, Gerhard A. Lustigman, Sara Herbert, De'Broski R. Keiser, Paul B. Nutman, Thomas B. Krolewiecki, Alejandro J. Abraham, David |
| description | Protective immunity to larval Strongyloides stercoralis in mice has been shown to be dependent on antibody, complement, and granulocytes. The goals of the present study was to determine the following: (1) whether human serum could passively transfer immunity to mice, (2) the mechanism by which the serum mediated killing, and (3) whether the antigens (Ags) recognized by the protective human antibody could induce protective immunity in mice. Immunoglobulin G (IgG) from a S. stercoralis-seropositive individual passively transferred immunity to mice. The antibody required granulocytes, but not eosinophils, and complement activation to kill the larvae. Antibody-dependent cellular cytotoxicity was not required for larval killing. Immunization of mice with soluble larval Ags isolated by use of the protective immune IgG resulted in protective immunity. In conclusion, immunity could be transferred to mice by IgG from immune humans, and Ags identified by the immune human IgG induced protective immunity in mice, which thereby suggests their possible use in a vaccine against this infection. |
| doi_str_mv | 10.1086/382484 |
| format | Article |
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The goals of the present study was to determine the following: (1) whether human serum could passively transfer immunity to mice, (2) the mechanism by which the serum mediated killing, and (3) whether the antigens (Ags) recognized by the protective human antibody could induce protective immunity in mice. Immunoglobulin G (IgG) from a S. stercoralis-seropositive individual passively transferred immunity to mice. The antibody required granulocytes, but not eosinophils, and complement activation to kill the larvae. Antibody-dependent cellular cytotoxicity was not required for larval killing. Immunization of mice with soluble larval Ags isolated by use of the protective immune IgG resulted in protective immunity. In conclusion, immunity could be transferred to mice by IgG from immune humans, and Ags identified by the immune human IgG induced protective immunity in mice, which thereby suggests their possible use in a vaccine against this infection.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/382484</identifier><identifier>PMID: 15031798</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animals ; Antigens, Helminth - immunology ; Antigens, Helminth - isolation & purification ; Biological and medical sciences ; Blotting, Western ; Chromatography, Affinity ; Complement C3 - immunology ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunization, Passive - methods ; Immunoglobulin G - immunology ; Infectious diseases ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Knockout ; Microbiology ; Microscopy, Immunoelectron ; Receptors, IgG - immunology ; Strongyloides stercoralis - immunology ; Strongyloides stercoralis - ultrastructure ; Strongyloidiasis - immunology ; Strongyloidiasis - parasitology</subject><ispartof>The Journal of infectious diseases, 2004-04, Vol.189 (7), p.1282-1290</ispartof><rights>2004 by the Infectious Diseases Society of America 2004</rights><rights>2004 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Apr 1 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-dd70c76596431237acb539d11f4d2c370f05585c0bfc61a572db7ec554d5f03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15725535$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15031798$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kerepesi, Laura A.</creatorcontrib><creatorcontrib>Nolan, Thomas J.</creatorcontrib><creatorcontrib>Schad, Gerhard A.</creatorcontrib><creatorcontrib>Lustigman, Sara</creatorcontrib><creatorcontrib>Herbert, De'Broski R.</creatorcontrib><creatorcontrib>Keiser, Paul B.</creatorcontrib><creatorcontrib>Nutman, Thomas B.</creatorcontrib><creatorcontrib>Krolewiecki, Alejandro J.</creatorcontrib><creatorcontrib>Abraham, David</creatorcontrib><title>Human Immunoglobulin G Mediates Protective Immunity and Identifies Protective Antigens against Larval Strongyloides stercoralis in Mice</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><addtitle>J Infect Dis</addtitle><description>Protective immunity to larval Strongyloides stercoralis in mice has been shown to be dependent on antibody, complement, and granulocytes. The goals of the present study was to determine the following: (1) whether human serum could passively transfer immunity to mice, (2) the mechanism by which the serum mediated killing, and (3) whether the antigens (Ags) recognized by the protective human antibody could induce protective immunity in mice. Immunoglobulin G (IgG) from a S. stercoralis-seropositive individual passively transferred immunity to mice. The antibody required granulocytes, but not eosinophils, and complement activation to kill the larvae. Antibody-dependent cellular cytotoxicity was not required for larval killing. Immunization of mice with soluble larval Ags isolated by use of the protective immune IgG resulted in protective immunity. In conclusion, immunity could be transferred to mice by IgG from immune humans, and Ags identified by the immune human IgG induced protective immunity in mice, which thereby suggests their possible use in a vaccine against this infection.</description><subject>Animals</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Chromatography, Affinity</subject><subject>Complement C3 - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunization, Passive - methods</subject><subject>Immunoglobulin G - immunology</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Microbiology</subject><subject>Microscopy, Immunoelectron</subject><subject>Receptors, IgG - immunology</subject><subject>Strongyloides stercoralis - immunology</subject><subject>Strongyloides stercoralis - ultrastructure</subject><subject>Strongyloidiasis - immunology</subject><subject>Strongyloidiasis - parasitology</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10d2KEzEYBuAgiltXvQSJgp6N5meSzBwuRdtKF4UuKHsSMkmmZM0k3SSz2Cvwth2Z4qrgUSA8eb_wvQA8x-gtRg1_RxtSN_UDsMCMiopzTB-CBUKEVLhp2zPwJOcbhFBNuXgMzjBDFIu2WYAf63FQAW6GYQxx72M3ehfgCl5a41SxGX5OsVhd3J2dkStHqIKBG2NDcb37m1xMd3sbMlR75UIucKvSnfJwV1IM-6OPzkwPcrFJx6S8y3Cadum0fQoe9cpn--x0noPdh_dXy3W1_bTaLC-2laYtL5UxAmnBWctrigkVSneMtgbjvjZEU4F6xFjDNOp6zbFigphOWM1YbViP6Dl4M6ceUrwdbS5ycFlb71WwccxSYEFrKsgEX_0Db-KYwvQzSQhtMRaI36fpFHNOtpeH5AaVjhIj-asVObcywRentLEbrLlnpxom8PoEVNbK90kF7fIfThDGKJvcy9nF8fD_YdVs3LTm77-VSt8kF1Qwuf56LZc78eXj9epKruhP_KKumQ</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Kerepesi, Laura A.</creator><creator>Nolan, Thomas J.</creator><creator>Schad, Gerhard A.</creator><creator>Lustigman, Sara</creator><creator>Herbert, De'Broski R.</creator><creator>Keiser, Paul B.</creator><creator>Nutman, Thomas B.</creator><creator>Krolewiecki, Alejandro J.</creator><creator>Abraham, David</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Human Immunoglobulin G Mediates Protective Immunity and Identifies Protective Antigens against Larval Strongyloides stercoralis in Mice</title><author>Kerepesi, Laura A. ; Nolan, Thomas J. ; Schad, Gerhard A. ; Lustigman, Sara ; Herbert, De'Broski R. ; Keiser, Paul B. ; Nutman, Thomas B. ; Krolewiecki, Alejandro J. ; Abraham, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-dd70c76596431237acb539d11f4d2c370f05585c0bfc61a572db7ec554d5f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antigens, Helminth - immunology</topic><topic>Antigens, Helminth - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Chromatography, Affinity</topic><topic>Complement C3 - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunization, Passive - methods</topic><topic>Immunoglobulin G - immunology</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Microbiology</topic><topic>Microscopy, Immunoelectron</topic><topic>Receptors, IgG - immunology</topic><topic>Strongyloides stercoralis - immunology</topic><topic>Strongyloides stercoralis - ultrastructure</topic><topic>Strongyloidiasis - immunology</topic><topic>Strongyloidiasis - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kerepesi, Laura A.</creatorcontrib><creatorcontrib>Nolan, Thomas J.</creatorcontrib><creatorcontrib>Schad, Gerhard A.</creatorcontrib><creatorcontrib>Lustigman, Sara</creatorcontrib><creatorcontrib>Herbert, De'Broski R.</creatorcontrib><creatorcontrib>Keiser, Paul B.</creatorcontrib><creatorcontrib>Nutman, Thomas B.</creatorcontrib><creatorcontrib>Krolewiecki, Alejandro J.</creatorcontrib><creatorcontrib>Abraham, David</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kerepesi, Laura A.</au><au>Nolan, Thomas J.</au><au>Schad, Gerhard A.</au><au>Lustigman, Sara</au><au>Herbert, De'Broski R.</au><au>Keiser, Paul B.</au><au>Nutman, Thomas B.</au><au>Krolewiecki, Alejandro J.</au><au>Abraham, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Immunoglobulin G Mediates Protective Immunity and Identifies Protective Antigens against Larval Strongyloides stercoralis in Mice</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>J Infect Dis</stitle><addtitle>J Infect Dis</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>189</volume><issue>7</issue><spage>1282</spage><epage>1290</epage><pages>1282-1290</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Protective immunity to larval Strongyloides stercoralis in mice has been shown to be dependent on antibody, complement, and granulocytes. The goals of the present study was to determine the following: (1) whether human serum could passively transfer immunity to mice, (2) the mechanism by which the serum mediated killing, and (3) whether the antigens (Ags) recognized by the protective human antibody could induce protective immunity in mice. Immunoglobulin G (IgG) from a S. stercoralis-seropositive individual passively transferred immunity to mice. The antibody required granulocytes, but not eosinophils, and complement activation to kill the larvae. Antibody-dependent cellular cytotoxicity was not required for larval killing. Immunization of mice with soluble larval Ags isolated by use of the protective immune IgG resulted in protective immunity. In conclusion, immunity could be transferred to mice by IgG from immune humans, and Ags identified by the immune human IgG induced protective immunity in mice, which thereby suggests their possible use in a vaccine against this infection.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>15031798</pmid><doi>10.1086/382484</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals Antigens, Helminth - immunology Antigens, Helminth - isolation & purification Biological and medical sciences Blotting, Western Chromatography, Affinity Complement C3 - immunology Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Humans Immunization, Passive - methods Immunoglobulin G - immunology Infectious diseases Male Medical sciences Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Microbiology Microscopy, Immunoelectron Receptors, IgG - immunology Strongyloides stercoralis - immunology Strongyloides stercoralis - ultrastructure Strongyloidiasis - immunology Strongyloidiasis - parasitology |
| title | Human Immunoglobulin G Mediates Protective Immunity and Identifies Protective Antigens against Larval Strongyloides stercoralis in Mice |
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