Synthesis and Characterization of a Biotin-Alginate Conjugate and Its Application in a Biosensor Construction
Biotin was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a biotin-alginate conjugate for subsequent use in biosensor applications. The synthetic procedure was optimized with respect to pH of the reaction medium (pH 6.0...
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| Veröffentlicht in: | Biomacromolecules 2004-03, Vol.5 (2), p.389-396 |
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| creator | Polyak, Boris Geresh, Shimona Marks, Robert S |
| description | Biotin was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a biotin-alginate conjugate for subsequent use in biosensor applications. The synthetic procedure was optimized with respect to pH of the reaction medium (pH 6.0), the degree of uronic acid activation (20%), and the order of addition of the reagents. The biotin-alginate conjugate was characterized by titration with 2-anilinonaphthalene-6-sulfonic acid (2,6-ANS), 4-hydroxyazobene-2‘-carboxylic acid (HABA) and by an HPSEC-MALLS analytical method as well as by FTIR and 13C NMR spectroscopy. As a compromise between the need for a high percent of molar modification of the alginate, on one hand, and sufficient gelling capability, on the other hand, an optimal modification of 10−13% of biotin-alginate was used. The new biotin-alginate conjugate was used for the encapsulation of bioluminescent reporter cells into microspheres. A biosensor was prepared by conjugating these biotinylated alginate microspheres to the surface of a streptavidin-coated optical fiber, and the performance of the biosensor was demonstrated in the determination of the antibiotic, mitomycin C as a model toxin. |
| doi_str_mv | 10.1021/bm034454a |
| format | Article |
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The synthetic procedure was optimized with respect to pH of the reaction medium (pH 6.0), the degree of uronic acid activation (20%), and the order of addition of the reagents. The biotin-alginate conjugate was characterized by titration with 2-anilinonaphthalene-6-sulfonic acid (2,6-ANS), 4-hydroxyazobene-2‘-carboxylic acid (HABA) and by an HPSEC-MALLS analytical method as well as by FTIR and 13C NMR spectroscopy. As a compromise between the need for a high percent of molar modification of the alginate, on one hand, and sufficient gelling capability, on the other hand, an optimal modification of 10−13% of biotin-alginate was used. The new biotin-alginate conjugate was used for the encapsulation of bioluminescent reporter cells into microspheres. A biosensor was prepared by conjugating these biotinylated alginate microspheres to the surface of a streptavidin-coated optical fiber, and the performance of the biosensor was demonstrated in the determination of the antibiotic, mitomycin C as a model toxin.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm034454a</identifier><identifier>PMID: 15002998</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Alginates - analysis ; Alginates - chemical synthesis ; Applied sciences ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Biotin - analysis ; Biotin - chemical synthesis ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Glucuronic Acid - analysis ; Glucuronic Acid - chemical synthesis ; Hexuronic Acids - analysis ; Hexuronic Acids - chemical synthesis ; Methods. 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The synthetic procedure was optimized with respect to pH of the reaction medium (pH 6.0), the degree of uronic acid activation (20%), and the order of addition of the reagents. The biotin-alginate conjugate was characterized by titration with 2-anilinonaphthalene-6-sulfonic acid (2,6-ANS), 4-hydroxyazobene-2‘-carboxylic acid (HABA) and by an HPSEC-MALLS analytical method as well as by FTIR and 13C NMR spectroscopy. As a compromise between the need for a high percent of molar modification of the alginate, on one hand, and sufficient gelling capability, on the other hand, an optimal modification of 10−13% of biotin-alginate was used. The new biotin-alginate conjugate was used for the encapsulation of bioluminescent reporter cells into microspheres. 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Technologies</subject><subject>Microspheres</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><subject>Starch and polysaccharides</subject><subject>Various methods and equipments</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cFO3DAQBmCrAsGy7aEvgHIBiUPAju3EOS6rliKtxIH2HE28NniV2IvHOSxPT8KuoIdKPXkkf_-MNEPId0avGS3YTdtTLoQU8IXMmCzKXJS0OHqvZV5VdXVKzhA3lNKaC3lCTpmktKhrNSP9486nZ4MOM_DrbPkMEXQy0b1CcsFnwWaQ3bqQnM8X3ZPzkEy2DH4zPE3VlLlPmC22287pfcT5fQSNxxAnjCkOevr7So4tdGi-Hd45-fPzx-_lr3z1cHe_XKxyEFylvGRC84qpSqvWqtLU1PLC1K2mrRQaWGFLVSjQ0lBecVuXCiRYVnJulVmXis_J5b7vNoaXwWBqeofadB14EwZsKlZxMS7hv3B0TNacj_BqD3UMiNHYZhtdD3HXMNpMR2g-jjDa80PToe3N-lMetj6CiwMA1NDZCF47_MtJqeQ49sOBxmYThujHpf1j4Buygpr7</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Polyak, Boris</creator><creator>Geresh, Shimona</creator><creator>Marks, Robert S</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040301</creationdate><title>Synthesis and Characterization of a Biotin-Alginate Conjugate and Its Application in a Biosensor Construction</title><author>Polyak, Boris ; Geresh, Shimona ; Marks, Robert S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a438t-614c37187c8bf86e90f32e9bc0b54ca12f6828ac5e0373f968a5af1633f8ed683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alginates - analysis</topic><topic>Alginates - chemical synthesis</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Biotin - analysis</topic><topic>Biotin - chemical synthesis</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucuronic Acid - analysis</topic><topic>Glucuronic Acid - chemical synthesis</topic><topic>Hexuronic Acids - analysis</topic><topic>Hexuronic Acids - chemical synthesis</topic><topic>Methods. Procedures. Technologies</topic><topic>Microspheres</topic><topic>Natural polymers</topic><topic>Physicochemistry of polymers</topic><topic>Starch and polysaccharides</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polyak, Boris</creatorcontrib><creatorcontrib>Geresh, Shimona</creatorcontrib><creatorcontrib>Marks, Robert S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomacromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polyak, Boris</au><au>Geresh, Shimona</au><au>Marks, Robert S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and Characterization of a Biotin-Alginate Conjugate and Its Application in a Biosensor Construction</atitle><jtitle>Biomacromolecules</jtitle><addtitle>Biomacromolecules</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>5</volume><issue>2</issue><spage>389</spage><epage>396</epage><pages>389-396</pages><issn>1525-7797</issn><eissn>1526-4602</eissn><abstract>Biotin was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a biotin-alginate conjugate for subsequent use in biosensor applications. 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| subjects | Alginates - analysis Alginates - chemical synthesis Applied sciences Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Biotin - analysis Biotin - chemical synthesis Exact sciences and technology Fundamental and applied biological sciences. Psychology Glucuronic Acid - analysis Glucuronic Acid - chemical synthesis Hexuronic Acids - analysis Hexuronic Acids - chemical synthesis Methods. Procedures. Technologies Microspheres Natural polymers Physicochemistry of polymers Starch and polysaccharides Various methods and equipments |
| title | Synthesis and Characterization of a Biotin-Alginate Conjugate and Its Application in a Biosensor Construction |
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