Improved platelet counting using two-dimensional laser light scatter
Clinical management of platelet disorders depends on accurate platelet counts. We evaluated a new analytic approach for platelet counting based on improved platelet discrimination. Current automated counting methods provide accurate platelet counts for most samples but often are unable to discrimina...
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| Veröffentlicht in: | American journal of clinical pathology 2000-08, Vol.114 (2), p.283-289 |
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| container_end_page | 289 |
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| container_issue | 2 |
| container_start_page | 283 |
| container_title | American journal of clinical pathology |
| container_volume | 114 |
| creator | KUNICKA, J. E FISCHER, G MURPHY, J ZELMANOVIC, D |
| description | Clinical management of platelet disorders depends on accurate platelet counts. We evaluated a new analytic approach for platelet counting based on improved platelet discrimination. Current automated counting methods provide accurate platelet counts for most samples but often are unable to discriminate platelets accurately from nonplatelet particles such as microcytic RBCs, RBC fragments, and cellular debris that may falsely elevate platelet counts. The new approach measures 2 light-scatter angles of platelets and nonplatelet particles as they pass through a laser beam. The volume and refractive index of each platelet and particle are derived from the light-scatter measurements using the Mie scattering theory. Together, these 2 measurements provide improved platelet discrimination compared with 1-dimensional methods. With its improved discrimination, 2-dimensional platelet analysis provides more accurate platelet counts in samples containing interfering particles and may contribute to more effective clinical management of patients with platelet disorders. |
| doi_str_mv | 10.1309/0GLP-K5NU-N1GD-PR8T |
| format | Article |
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The volume and refractive index of each platelet and particle are derived from the light-scatter measurements using the Mie scattering theory. Together, these 2 measurements provide improved platelet discrimination compared with 1-dimensional methods. With its improved discrimination, 2-dimensional platelet analysis provides more accurate platelet counts in samples containing interfering particles and may contribute to more effective clinical management of patients with platelet disorders.</description><subject>Biological and medical sciences</subject><subject>Blood Platelet Disorders - diagnosis</subject><subject>Blood Platelets - pathology</subject><subject>Evaluation Studies as Topic</subject><subject>False Positive Reactions</subject><subject>Hematology</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lasers</subject><subject>Light</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. 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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biological and medical sciences Blood Platelet Disorders - diagnosis Blood Platelets - pathology Evaluation Studies as Topic False Positive Reactions Hematology Humans Investigative techniques, diagnostic techniques (general aspects) Lasers Light Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Platelet Count - methods Reproducibility of Results Scattering, Radiation |
| title | Improved platelet counting using two-dimensional laser light scatter |
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