NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1
Abstract The polysialic acid capsule of Escherichia coli K1 is an essential virulence determinant. The kps gene cluster, which encodes the proteins necessary for polymer synthesis and transport, is divided into three functional regions. In this report, we present evidence that the neuD gene from reg...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 2000-08, Vol.189 (2), p.281-284 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 284 |
|---|---|
| container_issue | 2 |
| container_start_page | 281 |
| container_title | FEMS microbiology letters |
| container_volume | 189 |
| creator | Daines, Dayle A. Wright, Lori F. Chaffin, Donald O. Rubens, Craig E. Silver, Richard P. |
| description | Abstract
The polysialic acid capsule of Escherichia coli K1 is an essential virulence determinant. The kps gene cluster, which encodes the proteins necessary for polymer synthesis and transport, is divided into three functional regions. In this report, we present evidence that the neuD gene from region 2 is involved in sialic acid synthesis. A non-polar chromosomal deletion in neuD was constructed. The defect was complemented by neuD in trans or by the addition of exogenous sialic acid. A NeuD homologue, NeuIIID, from serotype III Streptococcus agalactiae (GBS) also restored capsule expression to the neuD deletion strain. These data confirm the role of neuD in E. coli sialic acid capsule synthesis and demonstrate that the neuIIID homologue from GBS shares a similar enzymatic function. |
| doi_str_mv | 10.1111/j.1574-6968.2000.tb09244.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71731317</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6968.2000.tb09244.x</oup_id><sourcerecordid>71731317</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4041-beda2769e40563bfe4beb47b0dc41f7b090ef00afccd4b2725b3488252dbd1f93</originalsourceid><addsrcrecordid>eNqVkF9r2zAUxcVYWdNsX2GIDfZm90qWLXsPhZL-pen2sj0LSZaIgmKnUsyab18Zh1LK-lA96ILuOUeHH0LfCOQkndN1TkrOsqqp6pwCQL5T0FDG8scPaPa8-ohmUPA6I9DwY3QS4zpJGYXqEzpObwXwks7QzS8zXOCtl_uIJQ69N9h1eLcyOO67NKKLuLc4OumdxlK7dtxfRr0ywemVk1j33uE78hkdWemj-XKYc_T36vLP4iZb_r6-XZwvM82AkUyZVlJeNYZBWRXKGqaMYlxBqxmxaTZgLIC0WrdMUU5LVbC6piVtVUtsU8zRjyl3G_qHwcSd2LiojfeyM_0QBSe8IEW65uj7K-G6H0KXuglaEAIVZwBJ9XNS6dDHGIwV2-A2MuwFATHSFmsxIhUjUjHSFgfa4jGZvx6-GNTGtC-sE94XHWTU0tsgO-3is64BIM3Y4WxS_XPe7N9RQFzdL2lNUkA5BfTD9g179r_-T7F5qao</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2311067400</pqid></control><display><type>article</type><title>NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Daines, Dayle A. ; Wright, Lori F. ; Chaffin, Donald O. ; Rubens, Craig E. ; Silver, Richard P.</creator><creatorcontrib>Daines, Dayle A. ; Wright, Lori F. ; Chaffin, Donald O. ; Rubens, Craig E. ; Silver, Richard P.</creatorcontrib><description>Abstract
The polysialic acid capsule of Escherichia coli K1 is an essential virulence determinant. The kps gene cluster, which encodes the proteins necessary for polymer synthesis and transport, is divided into three functional regions. In this report, we present evidence that the neuD gene from region 2 is involved in sialic acid synthesis. A non-polar chromosomal deletion in neuD was constructed. The defect was complemented by neuD in trans or by the addition of exogenous sialic acid. A NeuD homologue, NeuIIID, from serotype III Streptococcus agalactiae (GBS) also restored capsule expression to the neuD deletion strain. These data confirm the role of neuD in E. coli sialic acid capsule synthesis and demonstrate that the neuIIID homologue from GBS shares a similar enzymatic function.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2000.tb09244.x</identifier><identifier>PMID: 10930752</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetyltransferases - metabolism ; Acids ; Baby foods ; Bacteria ; Bacteriology ; Biological and medical sciences ; Chemical synthesis ; Chromosome deletion ; E coli ; Escherichia coli ; Escherichia coli - metabolism ; Escherichia coli Proteins ; Fundamental and applied biological sciences. Psychology ; Group B Streptococcus ; Homology ; K1 capsule ; Membrane Proteins - metabolism ; Metabolism. Enzymes ; Microbiology ; N-Acetylneuraminic Acid - metabolism ; NeuD ; Polymers ; Polysialic acid ; Sialic acid ; Streptococcus infections ; Virulence</subject><ispartof>FEMS microbiology letters, 2000-08, Vol.189 (2), p.281-284</ispartof><rights>2000 Federation of European Microbiological Societies 2000</rights><rights>2001 INIST-CNRS</rights><rights>2000 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4041-beda2769e40563bfe4beb47b0dc41f7b090ef00afccd4b2725b3488252dbd1f93</citedby><cites>FETCH-LOGICAL-c4041-beda2769e40563bfe4beb47b0dc41f7b090ef00afccd4b2725b3488252dbd1f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2000.tb09244.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2000.tb09244.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=900190$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10930752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Daines, Dayle A.</creatorcontrib><creatorcontrib>Wright, Lori F.</creatorcontrib><creatorcontrib>Chaffin, Donald O.</creatorcontrib><creatorcontrib>Rubens, Craig E.</creatorcontrib><creatorcontrib>Silver, Richard P.</creatorcontrib><title>NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
The polysialic acid capsule of Escherichia coli K1 is an essential virulence determinant. The kps gene cluster, which encodes the proteins necessary for polymer synthesis and transport, is divided into three functional regions. In this report, we present evidence that the neuD gene from region 2 is involved in sialic acid synthesis. A non-polar chromosomal deletion in neuD was constructed. The defect was complemented by neuD in trans or by the addition of exogenous sialic acid. A NeuD homologue, NeuIIID, from serotype III Streptococcus agalactiae (GBS) also restored capsule expression to the neuD deletion strain. These data confirm the role of neuD in E. coli sialic acid capsule synthesis and demonstrate that the neuIIID homologue from GBS shares a similar enzymatic function.</description><subject>Acetyltransferases - metabolism</subject><subject>Acids</subject><subject>Baby foods</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chemical synthesis</subject><subject>Chromosome deletion</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Group B Streptococcus</subject><subject>Homology</subject><subject>K1 capsule</subject><subject>Membrane Proteins - metabolism</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>N-Acetylneuraminic Acid - metabolism</subject><subject>NeuD</subject><subject>Polymers</subject><subject>Polysialic acid</subject><subject>Sialic acid</subject><subject>Streptococcus infections</subject><subject>Virulence</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkF9r2zAUxcVYWdNsX2GIDfZm90qWLXsPhZL-pen2sj0LSZaIgmKnUsyab18Zh1LK-lA96ILuOUeHH0LfCOQkndN1TkrOsqqp6pwCQL5T0FDG8scPaPa8-ohmUPA6I9DwY3QS4zpJGYXqEzpObwXwks7QzS8zXOCtl_uIJQ69N9h1eLcyOO67NKKLuLc4OumdxlK7dtxfRr0ywemVk1j33uE78hkdWemj-XKYc_T36vLP4iZb_r6-XZwvM82AkUyZVlJeNYZBWRXKGqaMYlxBqxmxaTZgLIC0WrdMUU5LVbC6piVtVUtsU8zRjyl3G_qHwcSd2LiojfeyM_0QBSe8IEW65uj7K-G6H0KXuglaEAIVZwBJ9XNS6dDHGIwV2-A2MuwFATHSFmsxIhUjUjHSFgfa4jGZvx6-GNTGtC-sE94XHWTU0tsgO-3is64BIM3Y4WxS_XPe7N9RQFzdL2lNUkA5BfTD9g179r_-T7F5qao</recordid><startdate>200008</startdate><enddate>200008</enddate><creator>Daines, Dayle A.</creator><creator>Wright, Lori F.</creator><creator>Chaffin, Donald O.</creator><creator>Rubens, Craig E.</creator><creator>Silver, Richard P.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200008</creationdate><title>NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1</title><author>Daines, Dayle A. ; Wright, Lori F. ; Chaffin, Donald O. ; Rubens, Craig E. ; Silver, Richard P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4041-beda2769e40563bfe4beb47b0dc41f7b090ef00afccd4b2725b3488252dbd1f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acetyltransferases - metabolism</topic><topic>Acids</topic><topic>Baby foods</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chemical synthesis</topic><topic>Chromosome deletion</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Group B Streptococcus</topic><topic>Homology</topic><topic>K1 capsule</topic><topic>Membrane Proteins - metabolism</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>N-Acetylneuraminic Acid - metabolism</topic><topic>NeuD</topic><topic>Polymers</topic><topic>Polysialic acid</topic><topic>Sialic acid</topic><topic>Streptococcus infections</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Daines, Dayle A.</creatorcontrib><creatorcontrib>Wright, Lori F.</creatorcontrib><creatorcontrib>Chaffin, Donald O.</creatorcontrib><creatorcontrib>Rubens, Craig E.</creatorcontrib><creatorcontrib>Silver, Richard P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daines, Dayle A.</au><au>Wright, Lori F.</au><au>Chaffin, Donald O.</au><au>Rubens, Craig E.</au><au>Silver, Richard P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2000-08</date><risdate>2000</risdate><volume>189</volume><issue>2</issue><spage>281</spage><epage>284</epage><pages>281-284</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
The polysialic acid capsule of Escherichia coli K1 is an essential virulence determinant. The kps gene cluster, which encodes the proteins necessary for polymer synthesis and transport, is divided into three functional regions. In this report, we present evidence that the neuD gene from region 2 is involved in sialic acid synthesis. A non-polar chromosomal deletion in neuD was constructed. The defect was complemented by neuD in trans or by the addition of exogenous sialic acid. A NeuD homologue, NeuIIID, from serotype III Streptococcus agalactiae (GBS) also restored capsule expression to the neuD deletion strain. These data confirm the role of neuD in E. coli sialic acid capsule synthesis and demonstrate that the neuIIID homologue from GBS shares a similar enzymatic function.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10930752</pmid><doi>10.1111/j.1574-6968.2000.tb09244.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2000-08, Vol.189 (2), p.281-284 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71731317 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Acetyltransferases - metabolism Acids Baby foods Bacteria Bacteriology Biological and medical sciences Chemical synthesis Chromosome deletion E coli Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins Fundamental and applied biological sciences. Psychology Group B Streptococcus Homology K1 capsule Membrane Proteins - metabolism Metabolism. Enzymes Microbiology N-Acetylneuraminic Acid - metabolism NeuD Polymers Polysialic acid Sialic acid Streptococcus infections Virulence |
| title | NeuD plays a role in the synthesis of sialic acid in Escherichia coli K1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T06%3A29%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=NeuD%20plays%20a%20role%20in%20the%20synthesis%20of%20sialic%20acid%20in%20Escherichia%20coli%20K1&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Daines,%20Dayle%20A.&rft.date=2000-08&rft.volume=189&rft.issue=2&rft.spage=281&rft.epage=284&rft.pages=281-284&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.2000.tb09244.x&rft_dat=%3Cproquest_cross%3E71731317%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2311067400&rft_id=info:pmid/10930752&rft_oup_id=10.1111/j.1574-6968.2000.tb09244.x&rfr_iscdi=true |