Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium
Palate, lung, and nasal epithelium clone (Plunc, now renamed Splunc1) is a small secreted protein expressed in the oropharynx and upper airways of humans, mice, rats, and cows. This protein is structurally homologous to known mediators of host defense against gram-negative bacteria. We have characte...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2004-04, Vol.83 (4), p.658-666 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | LeClair, E.E Nomellini, V Bahena, M Singleton, V Bingle, L Craven, C.J Bingle, C.D |
| description | Palate, lung, and nasal epithelium clone (Plunc, now renamed Splunc1) is a small secreted protein expressed in the oropharynx and upper airways of humans, mice, rats, and cows. This protein is structurally homologous to known mediators of host defense against gram-negative bacteria. We have characterized the genomic sequence and expression of a novel but closely related gene from rodents, which we call
splunc5. Mouse Splunc5 sequence is 60% identical to mouse Splunc1. The genes also share highly conserved genomic elements including intron–exon structure and intronic sequence. Strikingly,
splunc5 is expressed exclusively in the interpapillary epithelium of the tongue's dorsal surface. By comparing the expression profiles of
splunc5,
splunc1, and a third related sequence,
lplunc1, in mice, we show that these genes are expressed in unique domains along a continuous corridor of oral, lingual, pharyngeal, and respiratory epithelia. This expression pattern is consistent with the hypothesis that these proteins protect epithelial surfaces colonized by potentially pathogenic microorganisms. |
| doi_str_mv | 10.1016/j.ygeno.2003.09.015 |
| format | Article |
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splunc5. Mouse Splunc5 sequence is 60% identical to mouse Splunc1. The genes also share highly conserved genomic elements including intron–exon structure and intronic sequence. Strikingly,
splunc5 is expressed exclusively in the interpapillary epithelium of the tongue's dorsal surface. By comparing the expression profiles of
splunc5,
splunc1, and a third related sequence,
lplunc1, in mice, we show that these genes are expressed in unique domains along a continuous corridor of oral, lingual, pharyngeal, and respiratory epithelia. This expression pattern is consistent with the hypothesis that these proteins protect epithelial surfaces colonized by potentially pathogenic microorganisms.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2003.09.015</identifier><identifier>PMID: 15028288</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Airway ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Duplication ; Epithelial Cells - metabolism ; Exons ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation ; Gene structure ; Genes. Genome ; Genetics of eukaryotes. Biological and molecular evolution ; Glycoproteins - biosynthesis ; Glycoproteins - genetics ; Host defense ; In Situ Hybridization ; Introns ; Mice ; Mice, Inbred C57BL ; Models, Genetic ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Multigene Family ; Phosphoproteins - biosynthesis ; Phosphoproteins - genetics ; Protein Biosynthesis ; Proteins - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - metabolism ; Salivary gland ; Salivary Glands - metabolism ; Sequence Homology, Nucleic Acid ; Tissue Distribution ; Tongue ; Tongue - metabolism</subject><ispartof>Genomics (San Diego, Calif.), 2004-04, Vol.83 (4), p.658-666</ispartof><rights>2003 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-d0bfdb4145da239bca920877147a2192122888e3e285cef2dd26314d763e2a7e3</citedby><cites>FETCH-LOGICAL-c416t-d0bfdb4145da239bca920877147a2192122888e3e285cef2dd26314d763e2a7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ygeno.2003.09.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15611782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15028288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LeClair, E.E</creatorcontrib><creatorcontrib>Nomellini, V</creatorcontrib><creatorcontrib>Bahena, M</creatorcontrib><creatorcontrib>Singleton, V</creatorcontrib><creatorcontrib>Bingle, L</creatorcontrib><creatorcontrib>Craven, C.J</creatorcontrib><creatorcontrib>Bingle, C.D</creatorcontrib><title>Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Palate, lung, and nasal epithelium clone (Plunc, now renamed Splunc1) is a small secreted protein expressed in the oropharynx and upper airways of humans, mice, rats, and cows. This protein is structurally homologous to known mediators of host defense against gram-negative bacteria. We have characterized the genomic sequence and expression of a novel but closely related gene from rodents, which we call
splunc5. Mouse Splunc5 sequence is 60% identical to mouse Splunc1. The genes also share highly conserved genomic elements including intron–exon structure and intronic sequence. Strikingly,
splunc5 is expressed exclusively in the interpapillary epithelium of the tongue's dorsal surface. By comparing the expression profiles of
splunc5,
splunc1, and a third related sequence,
lplunc1, in mice, we show that these genes are expressed in unique domains along a continuous corridor of oral, lingual, pharyngeal, and respiratory epithelia. This expression pattern is consistent with the hypothesis that these proteins protect epithelial surfaces colonized by potentially pathogenic microorganisms.</description><subject>Airway</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Duplication</subject><subject>Epithelial Cells - metabolism</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene structure</subject><subject>Genes. Genome</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Glycoproteins - biosynthesis</subject><subject>Glycoproteins - genetics</subject><subject>Host defense</subject><subject>In Situ Hybridization</subject><subject>Introns</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Models, Genetic</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Phosphoproteins - biosynthesis</subject><subject>Phosphoproteins - genetics</subject><subject>Protein Biosynthesis</subject><subject>Proteins - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - metabolism</subject><subject>Salivary gland</subject><subject>Salivary Glands - metabolism</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Tissue Distribution</subject><subject>Tongue</subject><subject>Tongue - metabolism</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS1ERYfCL0BC3sAuwY8kthcs0Kg8pBHtol1bjn0zeJTYg51UzL_H85BgBStb1985OtcHoTeU1JTQ7sOuPmwhxJoRwmuiakLbZ2hFiVSV7JruOVoRKWUl2oZfo5c57wghikv2Al3TljDJpFyhuB5j8GGLTXAYfu0T5OxjwHHABk9xyYAnmHpIx8n8A_D95vH7Gu9TnMEHPJjJj4citOOS_ROc7icTcLi8zzFsF8Cw90U7-mV6ha4GM2Z4fTlv0OPn24f112pz9-Xb-tOmsg3t5sqRfnB9Q5vWGcZVb41iRApBG2EYVYyykl4CByZbCwNzjnWcNk50ZWQE8Bv0_uxbkv5cIM968tnCOJoAZSstqOCEFvx_IBVKsYIWkJ9Bm2LOCQa9T34y6aAp0cdC9E6fCtHHQjRRuhRSVG8v9ks_gfujuTRQgHcXwGRrxiGZYH3-i-soFZIV7uOZg_JrTx6SztZDsOB8AjtrF_0_g_wGDESq9w</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>LeClair, E.E</creator><creator>Nomellini, V</creator><creator>Bahena, M</creator><creator>Singleton, V</creator><creator>Bingle, L</creator><creator>Craven, C.J</creator><creator>Bingle, C.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium</title><author>LeClair, E.E ; Nomellini, V ; Bahena, M ; Singleton, V ; Bingle, L ; Craven, C.J ; Bingle, C.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-d0bfdb4145da239bca920877147a2192122888e3e285cef2dd26314d763e2a7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Airway</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Duplication</topic><topic>Epithelial Cells - metabolism</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gene structure</topic><topic>Genes. Genome</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Glycoproteins - biosynthesis</topic><topic>Glycoproteins - genetics</topic><topic>Host defense</topic><topic>In Situ Hybridization</topic><topic>Introns</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Models, Genetic</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Phosphoproteins - biosynthesis</topic><topic>Phosphoproteins - genetics</topic><topic>Protein Biosynthesis</topic><topic>Proteins - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - metabolism</topic><topic>Salivary gland</topic><topic>Salivary Glands - metabolism</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Tissue Distribution</topic><topic>Tongue</topic><topic>Tongue - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LeClair, E.E</creatorcontrib><creatorcontrib>Nomellini, V</creatorcontrib><creatorcontrib>Bahena, M</creatorcontrib><creatorcontrib>Singleton, V</creatorcontrib><creatorcontrib>Bingle, L</creatorcontrib><creatorcontrib>Craven, C.J</creatorcontrib><creatorcontrib>Bingle, C.D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LeClair, E.E</au><au>Nomellini, V</au><au>Bahena, M</au><au>Singleton, V</au><au>Bingle, L</au><au>Craven, C.J</au><au>Bingle, C.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>83</volume><issue>4</issue><spage>658</spage><epage>666</epage><pages>658-666</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Palate, lung, and nasal epithelium clone (Plunc, now renamed Splunc1) is a small secreted protein expressed in the oropharynx and upper airways of humans, mice, rats, and cows. This protein is structurally homologous to known mediators of host defense against gram-negative bacteria. We have characterized the genomic sequence and expression of a novel but closely related gene from rodents, which we call
splunc5. Mouse Splunc5 sequence is 60% identical to mouse Splunc1. The genes also share highly conserved genomic elements including intron–exon structure and intronic sequence. Strikingly,
splunc5 is expressed exclusively in the interpapillary epithelium of the tongue's dorsal surface. By comparing the expression profiles of
splunc5,
splunc1, and a third related sequence,
lplunc1, in mice, we show that these genes are expressed in unique domains along a continuous corridor of oral, lingual, pharyngeal, and respiratory epithelia. This expression pattern is consistent with the hypothesis that these proteins protect epithelial surfaces colonized by potentially pathogenic microorganisms.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>15028288</pmid><doi>10.1016/j.ygeno.2003.09.015</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Airway Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cloning, Molecular Duplication Epithelial Cells - metabolism Exons Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation Gene structure Genes. Genome Genetics of eukaryotes. Biological and molecular evolution Glycoproteins - biosynthesis Glycoproteins - genetics Host defense In Situ Hybridization Introns Mice Mice, Inbred C57BL Models, Genetic Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family Phosphoproteins - biosynthesis Phosphoproteins - genetics Protein Biosynthesis Proteins - genetics Reverse Transcriptase Polymerase Chain Reaction RNA - metabolism Salivary gland Salivary Glands - metabolism Sequence Homology, Nucleic Acid Tissue Distribution Tongue Tongue - metabolism |
| title | Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium |
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