Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro
Ram spermatozoa are most susceptible to damage during freezing between the temperatures of −10°C and −25°C. The objectives of the present study were to examine how freezing rate through this critical temperature zone affected the fertility of spermatozoa as assessed in vivo and in vitro. Semen from...
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| Veröffentlicht in: | Animal reproduction science 2000-09, Vol.62 (4), p.265-275 |
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| creator | Byrne, G.P Lonergan, P Wade, M Duffy, P Donovan, A Hanrahan, J.P Boland, M.P |
| description | Ram spermatozoa are most susceptible to damage during freezing between the temperatures of −10°C and −25°C. The objectives of the present study were to examine how freezing rate through this critical temperature zone affected the fertility of spermatozoa as assessed in vivo and in vitro. Semen from six adult rams was frozen at two different rates (“fast”: 5°C/min from +5 to −25°C; “slow”: 0.5°C/min from +5 to −25°C). In Experiment 1, semen from the fast and slow treatments was used to fertilize ovine oocytes that had been matured in vitro. Semen from the fast treatment yielded a higher cleavage rate (57% vs. 26%;
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| doi_str_mv | 10.1016/S0378-4320(00)00121-4 |
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P<0.001) and more blastocysts per oocyte (28% vs. 13%,
P<0.001) than slow-frozen. No correlation was found between fertilizing ability and viability as assessed by fluorescent probes. Experiment 2 was designed to establish the conception rates following both cervical and intrauterine insemination of frozen-thawed semen from the same bank of semen as used in Experiment 1. Ewes were superovulated with FSH and inseminated by laparoscopy with frozen semen. A significant difference was found in the number of fertilized ova following embryo recovery (81.4% vs. 39.3%;
P<0.001). In a further study, 119 mature cull ewes were inseminated following a 12-day synchronization treatment with frozen semen by either intrauterine (laparoscopic) or cervical insemination. Insemination with fast-frozen semen resulted in a significantly higher pregnancy rate (
P<0.05) irrespective of method of insemination. The data show that freezing rate affects the proportion of spermatozoa that retain their fertilizing ability post-thawing. However, once fertilization has occurred, development to the blastocyst stage is independent of freezing rate.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/S0378-4320(00)00121-4</identifier><identifier>PMID: 10924829</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Centrifugation, Density Gradient - veterinary ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Embryo ; Female ; Fertility - physiology ; Fertilization in Vitro - methods ; Fertilization in Vitro - veterinary ; Fluorescent Dyes - chemistry ; IVF ; Laparoscopy - veterinary ; Male ; Microscopy, Fluorescence - veterinary ; Organic Chemicals ; Pregnancy ; Progestins - administration & dosage ; Propidium - chemistry ; Random Allocation ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sheep - physiology ; Sheep male reproduction ; Sperm Motility - physiology ; Sperm-Ovum Interactions ; Spermatozoa ; Spermatozoa - physiology ; Superovulation ; Temperature</subject><ispartof>Animal reproduction science, 2000-09, Vol.62 (4), p.265-275</ispartof><rights>2000 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-b440e7cbf64ca53e60ec5cda68fa7f3ce86edfd0f1d7a8c1387fcafcbb21d2083</citedby><cites>FETCH-LOGICAL-c361t-b440e7cbf64ca53e60ec5cda68fa7f3ce86edfd0f1d7a8c1387fcafcbb21d2083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-4320(00)00121-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10924829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Byrne, G.P</creatorcontrib><creatorcontrib>Lonergan, P</creatorcontrib><creatorcontrib>Wade, M</creatorcontrib><creatorcontrib>Duffy, P</creatorcontrib><creatorcontrib>Donovan, A</creatorcontrib><creatorcontrib>Hanrahan, J.P</creatorcontrib><creatorcontrib>Boland, M.P</creatorcontrib><title>Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>Ram spermatozoa are most susceptible to damage during freezing between the temperatures of −10°C and −25°C. The objectives of the present study were to examine how freezing rate through this critical temperature zone affected the fertility of spermatozoa as assessed in vivo and in vitro. Semen from six adult rams was frozen at two different rates (“fast”: 5°C/min from +5 to −25°C; “slow”: 0.5°C/min from +5 to −25°C). In Experiment 1, semen from the fast and slow treatments was used to fertilize ovine oocytes that had been matured in vitro. Semen from the fast treatment yielded a higher cleavage rate (57% vs. 26%;
P<0.001) and more blastocysts per oocyte (28% vs. 13%,
P<0.001) than slow-frozen. No correlation was found between fertilizing ability and viability as assessed by fluorescent probes. Experiment 2 was designed to establish the conception rates following both cervical and intrauterine insemination of frozen-thawed semen from the same bank of semen as used in Experiment 1. Ewes were superovulated with FSH and inseminated by laparoscopy with frozen semen. A significant difference was found in the number of fertilized ova following embryo recovery (81.4% vs. 39.3%;
P<0.001). In a further study, 119 mature cull ewes were inseminated following a 12-day synchronization treatment with frozen semen by either intrauterine (laparoscopic) or cervical insemination. Insemination with fast-frozen semen resulted in a significantly higher pregnancy rate (
P<0.05) irrespective of method of insemination. The data show that freezing rate affects the proportion of spermatozoa that retain their fertilizing ability post-thawing. However, once fertilization has occurred, development to the blastocyst stage is independent of freezing rate.</description><subject>Animals</subject><subject>Centrifugation, Density Gradient - veterinary</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Embryo</subject><subject>Female</subject><subject>Fertility - physiology</subject><subject>Fertilization in Vitro - methods</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Fluorescent Dyes - chemistry</subject><subject>IVF</subject><subject>Laparoscopy - veterinary</subject><subject>Male</subject><subject>Microscopy, Fluorescence - veterinary</subject><subject>Organic Chemicals</subject><subject>Pregnancy</subject><subject>Progestins - administration & dosage</subject><subject>Propidium - chemistry</subject><subject>Random Allocation</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sheep - physiology</subject><subject>Sheep male reproduction</subject><subject>Sperm Motility - physiology</subject><subject>Sperm-Ovum Interactions</subject><subject>Spermatozoa</subject><subject>Spermatozoa - physiology</subject><subject>Superovulation</subject><subject>Temperature</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQQIMouq7-BCUn0UN1knSb9iQifoHgQcVjSJOJRLbNmmQX9NfbtSLehIGZDC8zySPkgMEpA1adPYKQdVEKDscAJwCMs6LcIBNWS1FwLvgmmfwiO2Q3pTcAkFXVbJMdBg0va95MyMuVc2gyDY66iPjp-1cadcZ1I-qOpgXGTufwGTQNPU3LNuH7EvtMHcbs5z5_UN_TlV8Fqns71jmGPbLl9Dzh_k-ekufrq6fL2-L-4ebu8uK-MKJiuWjLElCa1lWl0TOBFaCZGaur2mnphMG6QussOGalrg0TtXRGO9O2nFkOtZiSo3HuIobhXSmrzieD87nuMSyTkkzypmz4AM5G0MSQUkSnFtF3On4oBmptVH0bVWtdCtYxGB1OU3L4s2DZdmj_3BoVDsD5CODwzZXHqJLx2Bu0Pg5mlQ3-nxVfWsqHnA</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Byrne, G.P</creator><creator>Lonergan, P</creator><creator>Wade, M</creator><creator>Duffy, P</creator><creator>Donovan, A</creator><creator>Hanrahan, J.P</creator><creator>Boland, M.P</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro</title><author>Byrne, G.P ; Lonergan, P ; Wade, M ; Duffy, P ; Donovan, A ; Hanrahan, J.P ; Boland, M.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-b440e7cbf64ca53e60ec5cda68fa7f3ce86edfd0f1d7a8c1387fcafcbb21d2083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Centrifugation, Density Gradient - veterinary</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Embryo</topic><topic>Female</topic><topic>Fertility - physiology</topic><topic>Fertilization in Vitro - methods</topic><topic>Fertilization in Vitro - veterinary</topic><topic>Fluorescent Dyes - chemistry</topic><topic>IVF</topic><topic>Laparoscopy - veterinary</topic><topic>Male</topic><topic>Microscopy, Fluorescence - veterinary</topic><topic>Organic Chemicals</topic><topic>Pregnancy</topic><topic>Progestins - administration & dosage</topic><topic>Propidium - chemistry</topic><topic>Random Allocation</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sheep - physiology</topic><topic>Sheep male reproduction</topic><topic>Sperm Motility - physiology</topic><topic>Sperm-Ovum Interactions</topic><topic>Spermatozoa</topic><topic>Spermatozoa - physiology</topic><topic>Superovulation</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Byrne, G.P</creatorcontrib><creatorcontrib>Lonergan, P</creatorcontrib><creatorcontrib>Wade, M</creatorcontrib><creatorcontrib>Duffy, P</creatorcontrib><creatorcontrib>Donovan, A</creatorcontrib><creatorcontrib>Hanrahan, J.P</creatorcontrib><creatorcontrib>Boland, M.P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Byrne, G.P</au><au>Lonergan, P</au><au>Wade, M</au><au>Duffy, P</au><au>Donovan, A</au><au>Hanrahan, J.P</au><au>Boland, M.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>62</volume><issue>4</issue><spage>265</spage><epage>275</epage><pages>265-275</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>Ram spermatozoa are most susceptible to damage during freezing between the temperatures of −10°C and −25°C. The objectives of the present study were to examine how freezing rate through this critical temperature zone affected the fertility of spermatozoa as assessed in vivo and in vitro. Semen from six adult rams was frozen at two different rates (“fast”: 5°C/min from +5 to −25°C; “slow”: 0.5°C/min from +5 to −25°C). In Experiment 1, semen from the fast and slow treatments was used to fertilize ovine oocytes that had been matured in vitro. Semen from the fast treatment yielded a higher cleavage rate (57% vs. 26%;
P<0.001) and more blastocysts per oocyte (28% vs. 13%,
P<0.001) than slow-frozen. No correlation was found between fertilizing ability and viability as assessed by fluorescent probes. Experiment 2 was designed to establish the conception rates following both cervical and intrauterine insemination of frozen-thawed semen from the same bank of semen as used in Experiment 1. Ewes were superovulated with FSH and inseminated by laparoscopy with frozen semen. A significant difference was found in the number of fertilized ova following embryo recovery (81.4% vs. 39.3%;
P<0.001). In a further study, 119 mature cull ewes were inseminated following a 12-day synchronization treatment with frozen semen by either intrauterine (laparoscopic) or cervical insemination. Insemination with fast-frozen semen resulted in a significantly higher pregnancy rate (
P<0.05) irrespective of method of insemination. The data show that freezing rate affects the proportion of spermatozoa that retain their fertilizing ability post-thawing. However, once fertilization has occurred, development to the blastocyst stage is independent of freezing rate.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10924829</pmid><doi>10.1016/S0378-4320(00)00121-4</doi><tpages>11</tpages></addata></record> |
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| ispartof | Animal reproduction science, 2000-09, Vol.62 (4), p.265-275 |
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| subjects | Animals Centrifugation, Density Gradient - veterinary Cryopreservation Cryopreservation - methods Cryopreservation - veterinary Embryo Female Fertility - physiology Fertilization in Vitro - methods Fertilization in Vitro - veterinary Fluorescent Dyes - chemistry IVF Laparoscopy - veterinary Male Microscopy, Fluorescence - veterinary Organic Chemicals Pregnancy Progestins - administration & dosage Propidium - chemistry Random Allocation Semen Preservation - methods Semen Preservation - veterinary Sheep - physiology Sheep male reproduction Sperm Motility - physiology Sperm-Ovum Interactions Spermatozoa Spermatozoa - physiology Superovulation Temperature |
| title | Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro |
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