Analysis of APAF-1 expression in human cutaneous melanoma progression
: APAF‐1 plays a pivotal role in mitochondria‐dependent apoptosis, binding to cytochrome c and favoring activation of caspase‐9. It has been shown that epigenetic silencing of the APAF‐1 gene is a common event in several metastatic melanoma cells in vitro. We determined, by Western blot, variation i...
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| Veröffentlicht in: | Experimental dermatology 2004-02, Vol.13 (2), p.93-97 |
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| creator | Baldi, Alfonso Santini, Daniele Russo, Patrizia Catricalà, Caterina Amantea, Ada Picardo, Mauro Tatangelo, Fabiana Botti, Gerardo Dragonetti, Emanuele Murace, Raffaele Tonini, Giuseppe Natali, Pier Giorgio Baldi, Feliciano Paggi, Marco G. |
| description | : APAF‐1 plays a pivotal role in mitochondria‐dependent apoptosis, binding to cytochrome c and favoring activation of caspase‐9. It has been shown that epigenetic silencing of the APAF‐1 gene is a common event in several metastatic melanoma cells in vitro. We determined, by Western blot, variation in the level of expression of APAF‐1 in several human melanoma cell lines and, by immunohistochemistry, in a group of 106 histological samples including benign and malignant melanocytic lesions. We observed APAF‐1 down‐regulation or loss of expression in two metastatic melanoma cell lines, compared to primary melanoma cell lines. The immunohistochemical analysis revealed a significant difference in APAF‐1 staining between nevi and melanomas. In addition, we found a significant negative correlation between APAF‐1 expression level and tumor thickness and between primary melanomas and metastases. We conclude that loss of APAF‐1 expression can be considered as an indicator of malignant transformation in melanoma. |
| doi_str_mv | 10.1111/j.0906-6705.2004.00136.x |
| format | Article |
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It has been shown that epigenetic silencing of the APAF‐1 gene is a common event in several metastatic melanoma cells in vitro. We determined, by Western blot, variation in the level of expression of APAF‐1 in several human melanoma cell lines and, by immunohistochemistry, in a group of 106 histological samples including benign and malignant melanocytic lesions. We observed APAF‐1 down‐regulation or loss of expression in two metastatic melanoma cell lines, compared to primary melanoma cell lines. The immunohistochemical analysis revealed a significant difference in APAF‐1 staining between nevi and melanomas. In addition, we found a significant negative correlation between APAF‐1 expression level and tumor thickness and between primary melanomas and metastases. We conclude that loss of APAF‐1 expression can be considered as an indicator of malignant transformation in melanoma.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/j.0906-6705.2004.00136.x</identifier><identifier>PMID: 15009102</identifier><language>eng</language><publisher>Oxford, UK; Malden, USA: Munksgaard International Publishers</publisher><subject>APAF-1 ; Apoptosis - genetics ; Apoptotic Protease-Activating Factor 1 ; Biological and medical sciences ; Biopsy ; Cell Line, Tumor ; Dermatology ; Humans ; Immunohistochemistry ; Medical sciences ; melanoma ; Melanoma - genetics ; Melanoma - pathology ; Mitochondria - genetics ; Neoplasm Metastasis - genetics ; Neoplasm Metastasis - pathology ; Predictive Value of Tests ; Proteins - genetics ; Retrospective Studies ; Skin Neoplasms - genetics ; Skin Neoplasms - pathology ; Tumor Cells, Cultured ; tumor progression ; Tumors of the skin and soft tissue. Premalignant lesions</subject><ispartof>Experimental dermatology, 2004-02, Vol.13 (2), p.93-97</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4346-9ac10d75238c0f35fd5429dada5401c88b36a93399856d1ab47269932bc6f49e3</citedby><cites>FETCH-LOGICAL-c4346-9ac10d75238c0f35fd5429dada5401c88b36a93399856d1ab47269932bc6f49e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.0906-6705.2004.00136.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.0906-6705.2004.00136.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15513552$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15009102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baldi, Alfonso</creatorcontrib><creatorcontrib>Santini, Daniele</creatorcontrib><creatorcontrib>Russo, Patrizia</creatorcontrib><creatorcontrib>Catricalà, Caterina</creatorcontrib><creatorcontrib>Amantea, Ada</creatorcontrib><creatorcontrib>Picardo, Mauro</creatorcontrib><creatorcontrib>Tatangelo, Fabiana</creatorcontrib><creatorcontrib>Botti, Gerardo</creatorcontrib><creatorcontrib>Dragonetti, Emanuele</creatorcontrib><creatorcontrib>Murace, Raffaele</creatorcontrib><creatorcontrib>Tonini, Giuseppe</creatorcontrib><creatorcontrib>Natali, Pier Giorgio</creatorcontrib><creatorcontrib>Baldi, Feliciano</creatorcontrib><creatorcontrib>Paggi, Marco G.</creatorcontrib><title>Analysis of APAF-1 expression in human cutaneous melanoma progression</title><title>Experimental dermatology</title><addtitle>Exp Dermatol</addtitle><description>: APAF‐1 plays a pivotal role in mitochondria‐dependent apoptosis, binding to cytochrome c and favoring activation of caspase‐9. It has been shown that epigenetic silencing of the APAF‐1 gene is a common event in several metastatic melanoma cells in vitro. We determined, by Western blot, variation in the level of expression of APAF‐1 in several human melanoma cell lines and, by immunohistochemistry, in a group of 106 histological samples including benign and malignant melanocytic lesions. We observed APAF‐1 down‐regulation or loss of expression in two metastatic melanoma cell lines, compared to primary melanoma cell lines. The immunohistochemical analysis revealed a significant difference in APAF‐1 staining between nevi and melanomas. In addition, we found a significant negative correlation between APAF‐1 expression level and tumor thickness and between primary melanomas and metastases. We conclude that loss of APAF‐1 expression can be considered as an indicator of malignant transformation in melanoma.</description><subject>APAF-1</subject><subject>Apoptosis - genetics</subject><subject>Apoptotic Protease-Activating Factor 1</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Cell Line, Tumor</subject><subject>Dermatology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>melanoma</subject><subject>Melanoma - genetics</subject><subject>Melanoma - pathology</subject><subject>Mitochondria - genetics</subject><subject>Neoplasm Metastasis - genetics</subject><subject>Neoplasm Metastasis - pathology</subject><subject>Predictive Value of Tests</subject><subject>Proteins - genetics</subject><subject>Retrospective Studies</subject><subject>Skin Neoplasms - genetics</subject><subject>Skin Neoplasms - pathology</subject><subject>Tumor Cells, Cultured</subject><subject>tumor progression</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkbtOwzAUhi0EgnJ5BeQFtoRjO3ZiiaWCcpEq6ACim-U6DqTkUuJGtG-PQyJgxIs9fL_Pr-8ghAmExJ-LZQgSRCBi4CEFiEIAwkS42UEjIgACEJTvotEPdIAOnVt6KGYx30cHhANIAnSEJuNKF1uXO1xneDwb3wQE282qsc7ldYXzCr-1pa6wade6snXrcGkLXdWlxqumfh24Y7SX6cLZk-E-Qs83k6eru2D6eHt_NZ4GJmKRCKQ2BNKYU5YYyBjPUh5RmepU8wiISZIFE1oyJmXCRUr0IoqpkJLRhRFZJC07Quf9v372R2vdWpW5M7Yo-m4qJjGljHMPJj1omtq5xmZq1eSlbraKgOoUqqXq7KjOjuoUqm-FauOjp8OMdlHa9Dc4OPPA2QBoZ3SRNboyufvDceIrdNxlz33mhd3-u4CazK_9w8eDPp67td38xHXz7jN-jerl4VbdzeksntJECfYFj8OZgw</recordid><startdate>200402</startdate><enddate>200402</enddate><creator>Baldi, Alfonso</creator><creator>Santini, Daniele</creator><creator>Russo, Patrizia</creator><creator>Catricalà, Caterina</creator><creator>Amantea, Ada</creator><creator>Picardo, Mauro</creator><creator>Tatangelo, Fabiana</creator><creator>Botti, Gerardo</creator><creator>Dragonetti, Emanuele</creator><creator>Murace, Raffaele</creator><creator>Tonini, Giuseppe</creator><creator>Natali, Pier Giorgio</creator><creator>Baldi, Feliciano</creator><creator>Paggi, Marco G.</creator><general>Munksgaard International Publishers</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200402</creationdate><title>Analysis of APAF-1 expression in human cutaneous melanoma progression</title><author>Baldi, Alfonso ; Santini, Daniele ; Russo, Patrizia ; Catricalà, Caterina ; Amantea, Ada ; Picardo, Mauro ; Tatangelo, Fabiana ; Botti, Gerardo ; Dragonetti, Emanuele ; Murace, Raffaele ; Tonini, Giuseppe ; Natali, Pier Giorgio ; Baldi, Feliciano ; Paggi, Marco G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4346-9ac10d75238c0f35fd5429dada5401c88b36a93399856d1ab47269932bc6f49e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>APAF-1</topic><topic>Apoptosis - genetics</topic><topic>Apoptotic Protease-Activating Factor 1</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Cell Line, Tumor</topic><topic>Dermatology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>melanoma</topic><topic>Melanoma - genetics</topic><topic>Melanoma - pathology</topic><topic>Mitochondria - genetics</topic><topic>Neoplasm Metastasis - genetics</topic><topic>Neoplasm Metastasis - pathology</topic><topic>Predictive Value of Tests</topic><topic>Proteins - genetics</topic><topic>Retrospective Studies</topic><topic>Skin Neoplasms - genetics</topic><topic>Skin Neoplasms - pathology</topic><topic>Tumor Cells, Cultured</topic><topic>tumor progression</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baldi, Alfonso</creatorcontrib><creatorcontrib>Santini, Daniele</creatorcontrib><creatorcontrib>Russo, Patrizia</creatorcontrib><creatorcontrib>Catricalà, Caterina</creatorcontrib><creatorcontrib>Amantea, Ada</creatorcontrib><creatorcontrib>Picardo, Mauro</creatorcontrib><creatorcontrib>Tatangelo, Fabiana</creatorcontrib><creatorcontrib>Botti, Gerardo</creatorcontrib><creatorcontrib>Dragonetti, Emanuele</creatorcontrib><creatorcontrib>Murace, Raffaele</creatorcontrib><creatorcontrib>Tonini, Giuseppe</creatorcontrib><creatorcontrib>Natali, Pier Giorgio</creatorcontrib><creatorcontrib>Baldi, Feliciano</creatorcontrib><creatorcontrib>Paggi, Marco G.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baldi, Alfonso</au><au>Santini, Daniele</au><au>Russo, Patrizia</au><au>Catricalà, Caterina</au><au>Amantea, Ada</au><au>Picardo, Mauro</au><au>Tatangelo, Fabiana</au><au>Botti, Gerardo</au><au>Dragonetti, Emanuele</au><au>Murace, Raffaele</au><au>Tonini, Giuseppe</au><au>Natali, Pier Giorgio</au><au>Baldi, Feliciano</au><au>Paggi, Marco G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of APAF-1 expression in human cutaneous melanoma progression</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2004-02</date><risdate>2004</risdate><volume>13</volume><issue>2</issue><spage>93</spage><epage>97</epage><pages>93-97</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>: APAF‐1 plays a pivotal role in mitochondria‐dependent apoptosis, binding to cytochrome c and favoring activation of caspase‐9. It has been shown that epigenetic silencing of the APAF‐1 gene is a common event in several metastatic melanoma cells in vitro. We determined, by Western blot, variation in the level of expression of APAF‐1 in several human melanoma cell lines and, by immunohistochemistry, in a group of 106 histological samples including benign and malignant melanocytic lesions. We observed APAF‐1 down‐regulation or loss of expression in two metastatic melanoma cell lines, compared to primary melanoma cell lines. The immunohistochemical analysis revealed a significant difference in APAF‐1 staining between nevi and melanomas. In addition, we found a significant negative correlation between APAF‐1 expression level and tumor thickness and between primary melanomas and metastases. We conclude that loss of APAF‐1 expression can be considered as an indicator of malignant transformation in melanoma.</abstract><cop>Oxford, UK; Malden, USA</cop><pub>Munksgaard International Publishers</pub><pmid>15009102</pmid><doi>10.1111/j.0906-6705.2004.00136.x</doi><tpages>5</tpages></addata></record> |
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| subjects | APAF-1 Apoptosis - genetics Apoptotic Protease-Activating Factor 1 Biological and medical sciences Biopsy Cell Line, Tumor Dermatology Humans Immunohistochemistry Medical sciences melanoma Melanoma - genetics Melanoma - pathology Mitochondria - genetics Neoplasm Metastasis - genetics Neoplasm Metastasis - pathology Predictive Value of Tests Proteins - genetics Retrospective Studies Skin Neoplasms - genetics Skin Neoplasms - pathology Tumor Cells, Cultured tumor progression Tumors of the skin and soft tissue. Premalignant lesions |
| title | Analysis of APAF-1 expression in human cutaneous melanoma progression |
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