Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays
We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhib...
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| Veröffentlicht in: | Molecular human reproduction 2002-06, Vol.8 (6), p.511-517 |
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| creator | Sha, Jiahao Zhou, Zuomin Li, Jianmin Yin, Lanlan Yang, Huanmin Hu, Gengxi Luo, Ming Chan, Hsiao Chang Zhou, Kaiya |
| description | We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1–4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models. |
| doi_str_mv | 10.1093/molehr/8.6.511 |
| format | Article |
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The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1–4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models.</description><identifier>ISSN: 1360-9947</identifier><identifier>ISSN: 1460-2407</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/8.6.511</identifier><identifier>PMID: 12029067</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adult ; Animals ; Biological and medical sciences ; cDNA array ; development ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Humans ; Male ; Mammalian male genital system ; Mice ; Molecular Sequence Data ; Morphology. Physiology ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; spermatogenesis ; Spermatogenesis - genetics ; Spermatogenesis - physiology ; testis ; Testis - growth & development ; Testis - physiology ; Vertebrates: reproduction</subject><ispartof>Molecular human reproduction, 2002-06, Vol.8 (6), p.511-517</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Jun 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-95052bff339204733572f3557cde785f336ec75e68871b7a8311b5faf4bbeb013</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13684025$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12029067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sha, Jiahao</creatorcontrib><creatorcontrib>Zhou, Zuomin</creatorcontrib><creatorcontrib>Li, Jianmin</creatorcontrib><creatorcontrib>Yin, Lanlan</creatorcontrib><creatorcontrib>Yang, Huanmin</creatorcontrib><creatorcontrib>Hu, Gengxi</creatorcontrib><creatorcontrib>Luo, Ming</creatorcontrib><creatorcontrib>Chan, Hsiao Chang</creatorcontrib><creatorcontrib>Zhou, Kaiya</creatorcontrib><creatorcontrib>Spermatogenesis study group</creatorcontrib><title>Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays</title><title>Molecular human reproduction</title><addtitle>Mol. Hum. Reprod</addtitle><description>We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1–4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models.</description><subject>Adult</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>cDNA array</subject><subject>development</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Humans</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>spermatogenesis</subject><subject>Spermatogenesis - genetics</subject><subject>Spermatogenesis - physiology</subject><subject>testis</subject><subject>Testis - growth & development</subject><subject>Testis - physiology</subject><subject>Vertebrates: reproduction</subject><issn>1360-9947</issn><issn>1460-2407</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1PFTEUxRsjEUS3Lk1jort59HPaWRJEISG4UULYNJ3OLRRn2mc7Y-C_t7z3IokbV72959eT23sQekfJipKOH01phLt8pFftSlL6Ah1Q0ZKGCaJe1prXuuuE2kevS7knhCom9Su0TxlhHWnVAcrnA8Q5-ODsHFLEyeMZyhwKHuA3jGk9VRnbOOCyhjzZOd1ChBJKk2G0Mwx4c8ch4rtlsnGDTmkpsPGpylJCvMXu8-Uxtjnbx_IG7Xk7Fni7Ow_Rjy-n30_OmotvX89Pji8aJ6Scm04SyXrvOe8YEYpzqZjnUio3gNKy9ltwSkKrtaK9sppT2ktvveh76Anlh-jT1ned06-lDmOmUByMo41QBzSqboMIrv8LVkoQwmUFP_wD3qclx_oJw5ismJSkQqst5HIqJYM36xwmmx8NJeYpM7PNzGjTmppZffB-57r0EwzP-C6kCnzcAbY4O_psowvlmeOtFoQ9jddsuVAX__BXt_mnqS5KmrPrGyOvWk3oDTeC_wGaD6-Q</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Sha, Jiahao</creator><creator>Zhou, Zuomin</creator><creator>Li, Jianmin</creator><creator>Yin, Lanlan</creator><creator>Yang, Huanmin</creator><creator>Hu, Gengxi</creator><creator>Luo, Ming</creator><creator>Chan, Hsiao Chang</creator><creator>Zhou, Kaiya</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays</title><author>Sha, Jiahao ; Zhou, Zuomin ; Li, Jianmin ; Yin, Lanlan ; Yang, Huanmin ; Hu, Gengxi ; Luo, Ming ; Chan, Hsiao Chang ; Zhou, Kaiya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-95052bff339204733572f3557cde785f336ec75e68871b7a8311b5faf4bbeb013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>cDNA array</topic><topic>development</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Humans</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>spermatogenesis</topic><topic>Spermatogenesis - genetics</topic><topic>Spermatogenesis - physiology</topic><topic>testis</topic><topic>Testis - growth & development</topic><topic>Testis - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sha, Jiahao</creatorcontrib><creatorcontrib>Zhou, Zuomin</creatorcontrib><creatorcontrib>Li, Jianmin</creatorcontrib><creatorcontrib>Yin, Lanlan</creatorcontrib><creatorcontrib>Yang, Huanmin</creatorcontrib><creatorcontrib>Hu, Gengxi</creatorcontrib><creatorcontrib>Luo, Ming</creatorcontrib><creatorcontrib>Chan, Hsiao Chang</creatorcontrib><creatorcontrib>Zhou, Kaiya</creatorcontrib><creatorcontrib>Spermatogenesis study group</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sha, Jiahao</au><au>Zhou, Zuomin</au><au>Li, Jianmin</au><au>Yin, Lanlan</au><au>Yang, Huanmin</au><au>Hu, Gengxi</au><au>Luo, Ming</au><au>Chan, Hsiao Chang</au><au>Zhou, Kaiya</au><aucorp>Spermatogenesis study group</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol. Hum. Reprod</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>8</volume><issue>6</issue><spage>511</spage><epage>517</epage><pages>511-517</pages><issn>1360-9947</issn><issn>1460-2407</issn><eissn>1460-2407</eissn><abstract>We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1–4 weeks. 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| subjects | Adult Animals Biological and medical sciences cDNA array development Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Humans Male Mammalian male genital system Mice Molecular Sequence Data Morphology. Physiology Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis RNA, Messenger - genetics RNA, Messenger - metabolism spermatogenesis Spermatogenesis - genetics Spermatogenesis - physiology testis Testis - growth & development Testis - physiology Vertebrates: reproduction |
| title | Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays |
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