Interactions of 3T3 fibroblasts and endothelial cells with defined pore features
The colonization of biodegradable polymer scaffolds with cell populations has been established as the foundation for the engineering of a number of tissues, including cartilage, liver, and bone. Within these scaffolds, the cells encounter a porous environment in which they must migrate across the co...
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| Veröffentlicht in: | Journal of biomedical materials research 2002-08, Vol.61 (2), p.212-217 |
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| container_title | Journal of biomedical materials research |
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| creator | Salem, A. K. Stevens, R. Pearson, R. G. Davies, M. C. Tendler, S. J. B. Roberts, C. J. Williams, P. M. Shakesheff, K. M. |
| description | The colonization of biodegradable polymer scaffolds with cell populations has been established as the foundation for the engineering of a number of tissues, including cartilage, liver, and bone. Within these scaffolds, the cells encounter a porous environment in which they must migrate across the convoluted polymer surface to generate a homogenous cell distribution. Predicting the interactions between cells and pores is important if scaffold characteristics are to be optimized. Therefore, we investigated the behavior of two model cell types over a range of defined pore features. These pore features range from 5 to 90 μm in diameter and have been fabricated by photolithographic techniques. Quantitatively, the behavior of the cells is dependent on three factors: 1) percentage cell coverage of the surface; 2) pore size; and 3) cell type. Fibroblast cells displayed a co‐operative pattern of cell spreading in which pores with diameters greater than the cell dimensions were bridged by groups of cells using their neighbors as supports. Endothelial cells were unable to use neighbors as support structures and failed to bridge pores greater than the cell diameter. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 61: 212–217, 2002 |
| doi_str_mv | 10.1002/jbm.10195 |
| format | Article |
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These pore features range from 5 to 90 μm in diameter and have been fabricated by photolithographic techniques. Quantitatively, the behavior of the cells is dependent on three factors: 1) percentage cell coverage of the surface; 2) pore size; and 3) cell type. Fibroblast cells displayed a co‐operative pattern of cell spreading in which pores with diameters greater than the cell dimensions were bridged by groups of cells using their neighbors as supports. Endothelial cells were unable to use neighbors as support structures and failed to bridge pores greater than the cell diameter. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 61: 212–217, 2002</description><identifier>ISSN: 0021-9304</identifier><identifier>EISSN: 1097-4636</identifier><identifier>DOI: 10.1002/jbm.10195</identifier><identifier>PMID: 12007201</identifier><identifier>CODEN: JBMRBG</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>3T3 Cells - cytology ; 3T3 fibroblasts ; Actins - metabolism ; Actins - physiology ; Animals ; Biological and medical sciences ; Cattle ; Cell Culture Techniques ; Coated Materials, Biocompatible ; endothelial cells ; Endothelium, Vascular - cytology ; Fibroblasts - cytology ; Fibronectins ; Focal Adhesions ; Medical sciences ; Mice ; photolithography ; Porosity ; Silicon Compounds ; Surface Properties ; tissue engineering ; Tissue Engineering - methods</subject><ispartof>Journal of biomedical materials research, 2002-08, Vol.61 (2), p.212-217</ispartof><rights>Copyright © 2002 Wiley Periodicals, Inc.</rights><rights>2003 INIST-CNRS</rights><rights>Copyright 2002 Wiley Periodicals, Inc.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3895-24734e1c3a122f451d9efdab402a010529fc46bbc40b4e1d6e299ce053f9c9ca3</citedby><cites>FETCH-LOGICAL-c3895-24734e1c3a122f451d9efdab402a010529fc46bbc40b4e1d6e299ce053f9c9ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.10195$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.10195$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13705651$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12007201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salem, A. K.</creatorcontrib><creatorcontrib>Stevens, R.</creatorcontrib><creatorcontrib>Pearson, R. G.</creatorcontrib><creatorcontrib>Davies, M. C.</creatorcontrib><creatorcontrib>Tendler, S. J. B.</creatorcontrib><creatorcontrib>Roberts, C. J.</creatorcontrib><creatorcontrib>Williams, P. M.</creatorcontrib><creatorcontrib>Shakesheff, K. M.</creatorcontrib><title>Interactions of 3T3 fibroblasts and endothelial cells with defined pore features</title><title>Journal of biomedical materials research</title><addtitle>J. Biomed. Mater. Res</addtitle><description>The colonization of biodegradable polymer scaffolds with cell populations has been established as the foundation for the engineering of a number of tissues, including cartilage, liver, and bone. Within these scaffolds, the cells encounter a porous environment in which they must migrate across the convoluted polymer surface to generate a homogenous cell distribution. Predicting the interactions between cells and pores is important if scaffold characteristics are to be optimized. Therefore, we investigated the behavior of two model cell types over a range of defined pore features. These pore features range from 5 to 90 μm in diameter and have been fabricated by photolithographic techniques. Quantitatively, the behavior of the cells is dependent on three factors: 1) percentage cell coverage of the surface; 2) pore size; and 3) cell type. Fibroblast cells displayed a co‐operative pattern of cell spreading in which pores with diameters greater than the cell dimensions were bridged by groups of cells using their neighbors as supports. Endothelial cells were unable to use neighbors as support structures and failed to bridge pores greater than the cell diameter. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 61: 212–217, 2002</description><subject>3T3 Cells - cytology</subject><subject>3T3 fibroblasts</subject><subject>Actins - metabolism</subject><subject>Actins - physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Culture Techniques</subject><subject>Coated Materials, Biocompatible</subject><subject>endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Fibroblasts - cytology</subject><subject>Fibronectins</subject><subject>Focal Adhesions</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>photolithography</subject><subject>Porosity</subject><subject>Silicon Compounds</subject><subject>Surface Properties</subject><subject>tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>0021-9304</issn><issn>1097-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10D1PwzAQBmALgaB8DPwB5AUkhsDZjpN6hAoKCApDEWyW45yFIU2KnQr670lpgYnpLN1zd9ZLyD6DEwbAT1-LSfdgSq6RHgOVJ2kmsnXS63osUQLSLbId4ysAKCXYJtliHCDnwHrk4bpuMRjb-qaOtHFUjAV1vghNUZnYRmrqkmJdNu0LVt5U1GJVRfrh2xdaovM1lnTaBKQOTTsLGHfJhjNVxL1V3SGPlxfjwVVyez-8HpzdJlb0lUx4mosUmRWGce5SyUqFrjRFCtwAA8mVs2lWFDaFonNlhlwpiyCFU1ZZI3bI0XLvNDTvM4ytnvi4-JypsZlFnbOc9VWfdfB4CW1oYgzo9DT4iQlzzUAv4tNdfPo7vs4erJbOigmWf3KVVwcOV8BEayoXTG19_HMiB5nJhTtdug9f4fz_i_rm_O7ndLKc8LHFz98JE950lotc6qfRUN9djvqj57HUIL4A6TaVEg</recordid><startdate>200208</startdate><enddate>200208</enddate><creator>Salem, A. K.</creator><creator>Stevens, R.</creator><creator>Pearson, R. G.</creator><creator>Davies, M. C.</creator><creator>Tendler, S. J. B.</creator><creator>Roberts, C. J.</creator><creator>Williams, P. M.</creator><creator>Shakesheff, K. M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>John Wiley & Sons</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200208</creationdate><title>Interactions of 3T3 fibroblasts and endothelial cells with defined pore features</title><author>Salem, A. K. ; Stevens, R. ; Pearson, R. G. ; Davies, M. C. ; Tendler, S. J. B. ; Roberts, C. J. ; Williams, P. M. ; Shakesheff, K. 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K.</creatorcontrib><creatorcontrib>Stevens, R.</creatorcontrib><creatorcontrib>Pearson, R. G.</creatorcontrib><creatorcontrib>Davies, M. C.</creatorcontrib><creatorcontrib>Tendler, S. J. B.</creatorcontrib><creatorcontrib>Roberts, C. J.</creatorcontrib><creatorcontrib>Williams, P. M.</creatorcontrib><creatorcontrib>Shakesheff, K. M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salem, A. K.</au><au>Stevens, R.</au><au>Pearson, R. G.</au><au>Davies, M. C.</au><au>Tendler, S. J. B.</au><au>Roberts, C. 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Predicting the interactions between cells and pores is important if scaffold characteristics are to be optimized. Therefore, we investigated the behavior of two model cell types over a range of defined pore features. These pore features range from 5 to 90 μm in diameter and have been fabricated by photolithographic techniques. Quantitatively, the behavior of the cells is dependent on three factors: 1) percentage cell coverage of the surface; 2) pore size; and 3) cell type. Fibroblast cells displayed a co‐operative pattern of cell spreading in which pores with diameters greater than the cell dimensions were bridged by groups of cells using their neighbors as supports. Endothelial cells were unable to use neighbors as support structures and failed to bridge pores greater than the cell diameter. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 61: 212–217, 2002</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>12007201</pmid><doi>10.1002/jbm.10195</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3T3 Cells - cytology 3T3 fibroblasts Actins - metabolism Actins - physiology Animals Biological and medical sciences Cattle Cell Culture Techniques Coated Materials, Biocompatible endothelial cells Endothelium, Vascular - cytology Fibroblasts - cytology Fibronectins Focal Adhesions Medical sciences Mice photolithography Porosity Silicon Compounds Surface Properties tissue engineering Tissue Engineering - methods |
| title | Interactions of 3T3 fibroblasts and endothelial cells with defined pore features |
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