Purification and characterization of apolipophorin III from immune hemolymph of Heliothis virescens pupae

Apolipophorin III (ApoLp-III) from Heliothis virescens pupae was purified by heat-treatment followed by Sephadex G-50 filtration and reverse phase-HPLC. The molecular mass of the purified ApoLp-III was determined as 17 965.9±5 Da by mass spectrometry. The N-terminal sequence confirmed the protein as...

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Veröffentlicht in:	Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2002-06, Vol.132 (2), p.505-514
Hauptverfasser:	Chung, Kyung Tae, Ourth, Donald D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Apolipophorin III Apolipoproteins - chemistry Apolipoproteins - isolation & purification Apolipoproteins - metabolism Apolipoproteins - pharmacology Chromatography, Gel Chromatography, High Pressure Liquid Erythrocytes - metabolism Heliothis virescens Hemagglutination Hemagglutination Tests Hemolymph - chemistry Hemolymph - immunology Lectins - metabolism Lepidoptera - chemistry Lepidoptera - growth & development Lepidoptera - immunology Molecular Sequence Data Molecular Weight N-terminal sequence Pupa - chemistry Pupa - immunology Rabbits Sequence Alignment Sequence Analysis, Protein Spectrum Analysis UV absorption spectrum α-Helix
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