Effect of microenvironment pH of aluminum hydroxide adjuvant on the chemical stability of adsorbed antigen
The rate of acid-catalyzed hydrolysis of glucose-1-phosphate (G1P) when adsorbed to aluminum hydroxide adjuvant was significantly slower than the rate of hydrolysis of a solution of G1P at the same pH. It was concluded that the positively charged aluminum hydroxide adjuvant (iep 11.4) electrostatica...
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| Veröffentlicht in: | Vaccine 2004-03, Vol.22 (9), p.1172-1176 |
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| creator | Wittayanukulluk, Arunee Jiang, Dongping Regnier, Fred E Hem, Stanley L |
| description | The rate of acid-catalyzed hydrolysis of glucose-1-phosphate (G1P) when adsorbed to aluminum hydroxide adjuvant was significantly slower than the rate of hydrolysis of a solution of G1P at the same pH. It was concluded that the positively charged aluminum hydroxide adjuvant (iep 11.4) electrostatically attracted anions including hydroxyls to form a double layer surrounding the adjuvant particles. Thus, the pH of the microenvironment surrounding the aluminum hydroxide adjuvant was higher than the bulk pH. Adsorbed G1P hydrolyzed at a rate associated with the pH of the microenvironment of the surface of the adjuvant rather than with the pH of the bulk solution. Comparison of the rate constant for the hydrolysis of adsorbed G1P to the pH-stability profile of G1P in solution revealed that adsorbed G1P hydrolyzed at a rate associated with a pH that was approximately two pH units higher than the bulk pH. The results suggest that the chemical stability of antigens that degrade by pH-dependent mechanisms can be optimized by modifying the surface charge of the aluminum-containing adjuvant to produce the pH of maximum stability in the microenvironment of the adjuvant. |
| doi_str_mv | 10.1016/j.vaccine.2003.09.037 |
| format | Article |
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It was concluded that the positively charged aluminum hydroxide adjuvant (iep 11.4) electrostatically attracted anions including hydroxyls to form a double layer surrounding the adjuvant particles. Thus, the pH of the microenvironment surrounding the aluminum hydroxide adjuvant was higher than the bulk pH. Adsorbed G1P hydrolyzed at a rate associated with the pH of the microenvironment of the surface of the adjuvant rather than with the pH of the bulk solution. Comparison of the rate constant for the hydrolysis of adsorbed G1P to the pH-stability profile of G1P in solution revealed that adsorbed G1P hydrolyzed at a rate associated with a pH that was approximately two pH units higher than the bulk pH. The results suggest that the chemical stability of antigens that degrade by pH-dependent mechanisms can be optimized by modifying the surface charge of the aluminum-containing adjuvant to produce the pH of maximum stability in the microenvironment of the adjuvant.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2003.09.037</identifier><identifier>PMID: 15003645</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Absorption ; Adjuvants, Immunologic - chemistry ; Adsorption ; Aluminum ; Aluminum Hydroxide - chemistry ; Aluminum hydroxide adjuvant ; Anions ; Antigens ; Antigens - chemistry ; Applied microbiology ; Biological and medical sciences ; Colorimetry ; Fundamental and applied biological sciences. Psychology ; Glucose ; Glucose-1-phosphate hydrolysis ; Glucosephosphates - chemistry ; Hydrogen-Ion Concentration ; Hydrolysis ; Kinetics ; Microbiology ; Microenvironment pH ; Molybdenum - chemistry ; Studies ; Temperature ; Thermodynamics ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>Vaccine, 2004-03, Vol.22 (9), p.1172-1176</ispartof><rights>2003 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Elsevier Limited Mar 12, 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-6cfdeb839c927731f3ee00bcca3f3d5865dc1663508c8e9d07f1bc5727760cb53</citedby><cites>FETCH-LOGICAL-c450t-6cfdeb839c927731f3ee00bcca3f3d5865dc1663508c8e9d07f1bc5727760cb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1546938392?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976,64364,64366,64368,72218</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15580931$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15003645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wittayanukulluk, Arunee</creatorcontrib><creatorcontrib>Jiang, Dongping</creatorcontrib><creatorcontrib>Regnier, Fred E</creatorcontrib><creatorcontrib>Hem, Stanley L</creatorcontrib><title>Effect of microenvironment pH of aluminum hydroxide adjuvant on the chemical stability of adsorbed antigen</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>The rate of acid-catalyzed hydrolysis of glucose-1-phosphate (G1P) when adsorbed to aluminum hydroxide adjuvant was significantly slower than the rate of hydrolysis of a solution of G1P at the same pH. It was concluded that the positively charged aluminum hydroxide adjuvant (iep 11.4) electrostatically attracted anions including hydroxyls to form a double layer surrounding the adjuvant particles. Thus, the pH of the microenvironment surrounding the aluminum hydroxide adjuvant was higher than the bulk pH. Adsorbed G1P hydrolyzed at a rate associated with the pH of the microenvironment of the surface of the adjuvant rather than with the pH of the bulk solution. Comparison of the rate constant for the hydrolysis of adsorbed G1P to the pH-stability profile of G1P in solution revealed that adsorbed G1P hydrolyzed at a rate associated with a pH that was approximately two pH units higher than the bulk pH. The results suggest that the chemical stability of antigens that degrade by pH-dependent mechanisms can be optimized by modifying the surface charge of the aluminum-containing adjuvant to produce the pH of maximum stability in the microenvironment of the adjuvant.</description><subject>Absorption</subject><subject>Adjuvants, Immunologic - chemistry</subject><subject>Adsorption</subject><subject>Aluminum</subject><subject>Aluminum Hydroxide - chemistry</subject><subject>Aluminum hydroxide adjuvant</subject><subject>Anions</subject><subject>Antigens</subject><subject>Antigens - chemistry</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Colorimetry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose</subject><subject>Glucose-1-phosphate hydrolysis</subject><subject>Glucosephosphates - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Kinetics</subject><subject>Microbiology</subject><subject>Microenvironment pH</subject><subject>Molybdenum - chemistry</subject><subject>Studies</subject><subject>Temperature</subject><subject>Thermodynamics</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0UGL1DAUB_AgijuufgSlIHprfWmatD2JLKsrLHhR8BbS5MVJaZMxaQfn25txCoqXPQXC7_9I3p-QlxQqClS8G6uj0tp5rGoAVkFfAWsfkR3tWlbWnHaPyQ5q0ZQNhe9X5FlKIwBwRvun5IrynBEN35Hx1lrUSxFsMTsdA_qji8HP6JficHe-VtM6O7_Oxf5kYvjlDBbKjOtRZRF8seyx0HvMYTUVaVGDm9xy-hM0KcQBTZGl-4H-OXli1ZTwxXZek28fb7_e3JX3Xz59vvlwX-qGw1IKbQ0OHet1X7cto5YhAgxaK2aZ4Z3gRlMhGIdOd9gbaC0dNG8zFqAHzq7J28vcQww_V0yLnF3SOE3KY1iTbGlLqejZg5C2ddOJpsvw9X9wDGv0-ROS8iaPyq-ts-IXldeYUkQrD9HNKp4kBXnuTI5y60yeO5PQy9xZzr3apq_DjOZvaispgzcbUClv2UbltUv_ON5Bz2h27y8O83aPDqNM2qHXaFzMHUsT3ANP-Q1oMLgM</recordid><startdate>20040312</startdate><enddate>20040312</enddate><creator>Wittayanukulluk, Arunee</creator><creator>Jiang, Dongping</creator><creator>Regnier, Fred E</creator><creator>Hem, Stanley L</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040312</creationdate><title>Effect of microenvironment pH of aluminum hydroxide adjuvant on the chemical stability of adsorbed antigen</title><author>Wittayanukulluk, Arunee ; Jiang, Dongping ; Regnier, Fred E ; Hem, Stanley L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-6cfdeb839c927731f3ee00bcca3f3d5865dc1663508c8e9d07f1bc5727760cb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Absorption</topic><topic>Adjuvants, Immunologic - chemistry</topic><topic>Adsorption</topic><topic>Aluminum</topic><topic>Aluminum Hydroxide - chemistry</topic><topic>Aluminum hydroxide adjuvant</topic><topic>Anions</topic><topic>Antigens</topic><topic>Antigens - chemistry</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Colorimetry</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wittayanukulluk, Arunee</au><au>Jiang, Dongping</au><au>Regnier, Fred E</au><au>Hem, Stanley L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of microenvironment pH of aluminum hydroxide adjuvant on the chemical stability of adsorbed antigen</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2004-03-12</date><risdate>2004</risdate><volume>22</volume><issue>9</issue><spage>1172</spage><epage>1176</epage><pages>1172-1176</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>The rate of acid-catalyzed hydrolysis of glucose-1-phosphate (G1P) when adsorbed to aluminum hydroxide adjuvant was significantly slower than the rate of hydrolysis of a solution of G1P at the same pH. It was concluded that the positively charged aluminum hydroxide adjuvant (iep 11.4) electrostatically attracted anions including hydroxyls to form a double layer surrounding the adjuvant particles. Thus, the pH of the microenvironment surrounding the aluminum hydroxide adjuvant was higher than the bulk pH. Adsorbed G1P hydrolyzed at a rate associated with the pH of the microenvironment of the surface of the adjuvant rather than with the pH of the bulk solution. Comparison of the rate constant for the hydrolysis of adsorbed G1P to the pH-stability profile of G1P in solution revealed that adsorbed G1P hydrolyzed at a rate associated with a pH that was approximately two pH units higher than the bulk pH. The results suggest that the chemical stability of antigens that degrade by pH-dependent mechanisms can be optimized by modifying the surface charge of the aluminum-containing adjuvant to produce the pH of maximum stability in the microenvironment of the adjuvant.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15003645</pmid><doi>10.1016/j.vaccine.2003.09.037</doi><tpages>5</tpages></addata></record> |
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| ispartof | Vaccine, 2004-03, Vol.22 (9), p.1172-1176 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; ProQuest Central UK/Ireland |
| subjects | Absorption Adjuvants, Immunologic - chemistry Adsorption Aluminum Aluminum Hydroxide - chemistry Aluminum hydroxide adjuvant Anions Antigens Antigens - chemistry Applied microbiology Biological and medical sciences Colorimetry Fundamental and applied biological sciences. Psychology Glucose Glucose-1-phosphate hydrolysis Glucosephosphates - chemistry Hydrogen-Ion Concentration Hydrolysis Kinetics Microbiology Microenvironment pH Molybdenum - chemistry Studies Temperature Thermodynamics Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Effect of microenvironment pH of aluminum hydroxide adjuvant on the chemical stability of adsorbed antigen |
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