Complement-Like Protein TEP1 Is a Determinant of Vectorial Capacity in the Malaria Vector Anopheles gambiae
Anopheles mosquitoes are major vectors of human malaria in Africa. Large variation exists in the ability of mosquitoes to serve as vectors and to transmit malaria parasites, but the molecular mechanisms that determine vectorial capacity remain poorly understood. We report that the hemocyte-specific...
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| Veröffentlicht in: | Cell 2004-03, Vol.116 (5), p.661-670 |
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| creator | Blandin, Stephanie Shiao, Shin-Hong Moita, Luis F Janse, Chris J Waters, Andrew P Kafatos, Fotis C Levashina, Elena A |
| description | Anopheles mosquitoes are major vectors of human malaria in Africa. Large variation exists in the ability of mosquitoes to serve as vectors and to transmit malaria parasites, but the molecular mechanisms that determine vectorial capacity remain poorly understood. We report that the hemocyte-specific complement-like protein TEP1 from the mosquito
Anopheles gambiae binds to and mediates killing of midgut stages of the rodent malaria parasite
Plasmodium berghei. The dsRNA knockdown of
TEP1 in adults completely abolishes melanotic refractoriness in a genetically selected refractory strain. Moreover, in susceptible mosquitoes this knockdown increases the number of developing parasites. Our results suggest that the TEP1-dependent parasite killing is followed by a TEP1-independent clearance of dead parasites by lysis and/or melanization. Further elucidation of the molecular mechanisms of TEP1-mediated parasite killing will be of great importance for our understanding of the principles of vectorial capacity in insects. |
| doi_str_mv | 10.1016/S0092-8674(04)00173-4 |
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Anopheles gambiae binds to and mediates killing of midgut stages of the rodent malaria parasite
Plasmodium berghei. The dsRNA knockdown of
TEP1 in adults completely abolishes melanotic refractoriness in a genetically selected refractory strain. Moreover, in susceptible mosquitoes this knockdown increases the number of developing parasites. Our results suggest that the TEP1-dependent parasite killing is followed by a TEP1-independent clearance of dead parasites by lysis and/or melanization. Further elucidation of the molecular mechanisms of TEP1-mediated parasite killing will be of great importance for our understanding of the principles of vectorial capacity in insects.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/S0092-8674(04)00173-4</identifier><identifier>PMID: 15006349</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Anopheles - anatomy & histology ; Anopheles - metabolism ; Anopheles - parasitology ; Anopheles gambiae ; Female ; Humans ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insect Vectors - genetics ; Insect Vectors - parasitology ; Insect Vectors - physiology ; Malaria - epidemiology ; Models, Molecular ; Molecular Sequence Data ; Plasmodium berghei ; Plasmodium berghei - cytology ; Plasmodium berghei - physiology ; Polymorphism, Genetic ; Protein Structure, Tertiary ; RNA - metabolism ; Sequence Alignment</subject><ispartof>Cell, 2004-03, Vol.116 (5), p.661-670</ispartof><rights>2004 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-1b588226ad1067fa6761c921ed7a635be2980bb95b037c90d800293203cd2cfc3</citedby><cites>FETCH-LOGICAL-c491t-1b588226ad1067fa6761c921ed7a635be2980bb95b037c90d800293203cd2cfc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0092-8674(04)00173-4$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15006349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blandin, Stephanie</creatorcontrib><creatorcontrib>Shiao, Shin-Hong</creatorcontrib><creatorcontrib>Moita, Luis F</creatorcontrib><creatorcontrib>Janse, Chris J</creatorcontrib><creatorcontrib>Waters, Andrew P</creatorcontrib><creatorcontrib>Kafatos, Fotis C</creatorcontrib><creatorcontrib>Levashina, Elena A</creatorcontrib><title>Complement-Like Protein TEP1 Is a Determinant of Vectorial Capacity in the Malaria Vector Anopheles gambiae</title><title>Cell</title><addtitle>Cell</addtitle><description>Anopheles mosquitoes are major vectors of human malaria in Africa. Large variation exists in the ability of mosquitoes to serve as vectors and to transmit malaria parasites, but the molecular mechanisms that determine vectorial capacity remain poorly understood. We report that the hemocyte-specific complement-like protein TEP1 from the mosquito
Anopheles gambiae binds to and mediates killing of midgut stages of the rodent malaria parasite
Plasmodium berghei. The dsRNA knockdown of
TEP1 in adults completely abolishes melanotic refractoriness in a genetically selected refractory strain. Moreover, in susceptible mosquitoes this knockdown increases the number of developing parasites. Our results suggest that the TEP1-dependent parasite killing is followed by a TEP1-independent clearance of dead parasites by lysis and/or melanization. Further elucidation of the molecular mechanisms of TEP1-mediated parasite killing will be of great importance for our understanding of the principles of vectorial capacity in insects.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Anopheles - anatomy & histology</subject><subject>Anopheles - metabolism</subject><subject>Anopheles - parasitology</subject><subject>Anopheles gambiae</subject><subject>Female</subject><subject>Humans</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insect Vectors - genetics</subject><subject>Insect Vectors - parasitology</subject><subject>Insect Vectors - physiology</subject><subject>Malaria - epidemiology</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Plasmodium berghei</subject><subject>Plasmodium berghei - cytology</subject><subject>Plasmodium berghei - physiology</subject><subject>Polymorphism, Genetic</subject><subject>Protein Structure, Tertiary</subject><subject>RNA - metabolism</subject><subject>Sequence Alignment</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS1ERbeFnwDyCdFDytiJ7fiEqqVApUVUonC1HGdCTZM42F6k_nu87Ko9VhppDvO9N9J7hLxmcM6AyfffATSvWqmad9CcATBVV80zsmKgVdUwxZ-T1QNyTE5S-g0ArRDiBTlmAkDWjV6Ru3WYlhEnnHO18XdIr2PI6Gd6c3nN6FWiln7EjHHys50zDQP9iS6H6O1I13axzud7WvB8i_SrHW05HAh6MYflFkdM9JedOm_xJTka7Jjw1WGfkh-fLm_WX6rNt89X64tN5RrNcsU60bacS9szkGqwUknmNGfYKytr0SHXLXSdFh3UymnoWwCuaw6167kbXH1K3u59lxj-bDFlM_nkcBztjGGbjGKqGAv5JMiUZjWXdQHFHnQxpBRxMEv0k433hoHZ1WH-12F2WRsos6vDNEX35vBg203YP6oO-Rfgwx7Aksdfj9Ek53F22PtYUjR98E-8-Afx9ZiY</recordid><startdate>20040305</startdate><enddate>20040305</enddate><creator>Blandin, Stephanie</creator><creator>Shiao, Shin-Hong</creator><creator>Moita, Luis F</creator><creator>Janse, Chris J</creator><creator>Waters, Andrew P</creator><creator>Kafatos, Fotis C</creator><creator>Levashina, Elena A</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040305</creationdate><title>Complement-Like Protein TEP1 Is a Determinant of Vectorial Capacity in the Malaria Vector Anopheles gambiae</title><author>Blandin, Stephanie ; Shiao, Shin-Hong ; Moita, Luis F ; Janse, Chris J ; Waters, Andrew P ; Kafatos, Fotis C ; Levashina, Elena A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-1b588226ad1067fa6761c921ed7a635be2980bb95b037c90d800293203cd2cfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Anopheles - anatomy & histology</topic><topic>Anopheles - metabolism</topic><topic>Anopheles - parasitology</topic><topic>Anopheles gambiae</topic><topic>Female</topic><topic>Humans</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Insect Vectors - genetics</topic><topic>Insect Vectors - parasitology</topic><topic>Insect Vectors - physiology</topic><topic>Malaria - epidemiology</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Plasmodium berghei</topic><topic>Plasmodium berghei - cytology</topic><topic>Plasmodium berghei - physiology</topic><topic>Polymorphism, Genetic</topic><topic>Protein Structure, Tertiary</topic><topic>RNA - metabolism</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blandin, Stephanie</creatorcontrib><creatorcontrib>Shiao, Shin-Hong</creatorcontrib><creatorcontrib>Moita, Luis F</creatorcontrib><creatorcontrib>Janse, Chris J</creatorcontrib><creatorcontrib>Waters, Andrew P</creatorcontrib><creatorcontrib>Kafatos, Fotis C</creatorcontrib><creatorcontrib>Levashina, Elena A</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blandin, Stephanie</au><au>Shiao, Shin-Hong</au><au>Moita, Luis F</au><au>Janse, Chris J</au><au>Waters, Andrew P</au><au>Kafatos, Fotis C</au><au>Levashina, Elena A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complement-Like Protein TEP1 Is a Determinant of Vectorial Capacity in the Malaria Vector Anopheles gambiae</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>2004-03-05</date><risdate>2004</risdate><volume>116</volume><issue>5</issue><spage>661</spage><epage>670</epage><pages>661-670</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>Anopheles mosquitoes are major vectors of human malaria in Africa. Large variation exists in the ability of mosquitoes to serve as vectors and to transmit malaria parasites, but the molecular mechanisms that determine vectorial capacity remain poorly understood. We report that the hemocyte-specific complement-like protein TEP1 from the mosquito
Anopheles gambiae binds to and mediates killing of midgut stages of the rodent malaria parasite
Plasmodium berghei. The dsRNA knockdown of
TEP1 in adults completely abolishes melanotic refractoriness in a genetically selected refractory strain. Moreover, in susceptible mosquitoes this knockdown increases the number of developing parasites. Our results suggest that the TEP1-dependent parasite killing is followed by a TEP1-independent clearance of dead parasites by lysis and/or melanization. Further elucidation of the molecular mechanisms of TEP1-mediated parasite killing will be of great importance for our understanding of the principles of vectorial capacity in insects.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15006349</pmid><doi>10.1016/S0092-8674(04)00173-4</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals Anopheles - anatomy & histology Anopheles - metabolism Anopheles - parasitology Anopheles gambiae Female Humans Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism Insect Vectors - genetics Insect Vectors - parasitology Insect Vectors - physiology Malaria - epidemiology Models, Molecular Molecular Sequence Data Plasmodium berghei Plasmodium berghei - cytology Plasmodium berghei - physiology Polymorphism, Genetic Protein Structure, Tertiary RNA - metabolism Sequence Alignment |
| title | Complement-Like Protein TEP1 Is a Determinant of Vectorial Capacity in the Malaria Vector Anopheles gambiae |
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