The determination of protein phosphorylation on electrophoresis gel blots by laser ablation inductively coupled plasma-mass spectrometry
Laser ablation interfaced with inductively coupled plasma-mass spectrometry is described as a new method to determine the presence of phosphorylated proteins on electrophoresis gel blots. The method was applied to the phosphoprotein beta-casein with good detection levels being observed at 16 pmole....
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| Veröffentlicht in: | Analyst (London) 2002-04, Vol.127 (4), p.459-461 |
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| container_end_page | 461 |
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| container_issue | 4 |
| container_start_page | 459 |
| container_title | Analyst (London) |
| container_volume | 127 |
| creator | MARSHALL, Peter HEUDI, Olivier BAINS, Satty FREEMAN, Hamzah Neil ABOU-SHAKRA, Fadi REARDON, Kevin |
| description | Laser ablation interfaced with inductively coupled plasma-mass spectrometry is described as a new method to determine the presence of phosphorylated proteins on electrophoresis gel blots. The method was applied to the phosphoprotein beta-casein with good detection levels being observed at 16 pmole. Attempts at using the technique to detect beta-casein on electrophoresis gels are also described. |
| doi_str_mv | 10.1039/b201657p |
| format | Article |
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| ispartof | Analyst (London), 2002-04, Vol.127 (4), p.459-461 |
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| language | eng |
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| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Caseins - analysis Caseins - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Lasers Molecular biophysics Phosphorylation Photochemistry. Photosynthesis. Bioluminescence Radiation-biomolecule interaction |
| title | The determination of protein phosphorylation on electrophoresis gel blots by laser ablation inductively coupled plasma-mass spectrometry |
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