Cloning, Functional Expression, and Characterization of Cystatin in Sesame Seed

A cDNA fragment encoding cystatin, a cysteine protease inhibitor, was obtained from maturing sesame seeds. The clone was constructed in a nonfusion or fusion vector and then overexpressed in Escherichia coli. The recombinant cystatins were found in the soluble fraction of cell extract and were demon...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2004-03, Vol.52 (5), p.1350-1356
Hauptverfasser:	Shyu, Douglas J. H, Chou, Wing-Ming, Yiu, Tien-Joung, Lin, Coney P. C, Tzen, Jason T. C
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Amino Acid Sequence Biological and medical sciences Carrier Proteins - antagonists & inhibitors Cloning, Molecular Cystatins - chemistry Cystatins - genetics Cystatins - pharmacology DNA, Complementary - isolation & purification DNA, Plant - chemistry DNA, Plant - isolation & purification Economic plant physiology Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Molecular Sequence Data Morphology. Anatomy. Histology. Cytology Seeds - chemistry Sequence Alignment Sesamum - chemistry
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