Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium
We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in...
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| Veröffentlicht in: | Tree physiology 2004-05, Vol.24 (5), p.551-560 |
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| creator | Minocha, R Lee, J.S Long, S Bhatnagar, P Minocha, S.C |
| description | We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in transgenic and non-transgenic cells. Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein. |
| doi_str_mv | 10.1093/treephys/24.5.551 |
| format | Article |
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Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein.</description><identifier>ISSN: 0829-318X</identifier><identifier>EISSN: 1758-4469</identifier><identifier>DOI: 10.1093/treephys/24.5.551</identifier><identifier>PMID: 14996659</identifier><language>eng</language><publisher>Canada</publisher><subject>ammonium nitrate ; biochemical pathways ; biosynthesis ; cell membranes ; cell suspension culture ; Cells, Cultured ; chemical concentration ; chemical reactions ; Culture Media ; gene expression ; genes ; hybrids ; metabolism ; Nitrogen - metabolism ; nitrogen content ; nutrient requirements ; permeability ; Plants, Genetically Modified - cytology ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - physiology ; Populus - cytology ; Populus - genetics ; Populus - physiology ; Populus maximowiczii ; Populus nigra ; potassium nitrate ; protein synthesis ; putrescine ; Putrescine - biosynthesis ; transgenic plants ; Trees - cytology ; Trees - genetics ; Trees - physiology ; wild relatives</subject><ispartof>Tree physiology, 2004-05, Vol.24 (5), p.551-560</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-615b84e16e0a2af28fdd80cb7006403b92635a1b654c11409126c7c37102eb3b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14996659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minocha, R</creatorcontrib><creatorcontrib>Lee, J.S</creatorcontrib><creatorcontrib>Long, S</creatorcontrib><creatorcontrib>Bhatnagar, P</creatorcontrib><creatorcontrib>Minocha, S.C</creatorcontrib><title>Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium</title><title>Tree physiology</title><addtitle>Tree Physiol</addtitle><description>We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in transgenic and non-transgenic cells. Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein.</description><subject>ammonium nitrate</subject><subject>biochemical pathways</subject><subject>biosynthesis</subject><subject>cell membranes</subject><subject>cell suspension culture</subject><subject>Cells, Cultured</subject><subject>chemical concentration</subject><subject>chemical reactions</subject><subject>Culture Media</subject><subject>gene expression</subject><subject>genes</subject><subject>hybrids</subject><subject>metabolism</subject><subject>Nitrogen - metabolism</subject><subject>nitrogen content</subject><subject>nutrient requirements</subject><subject>permeability</subject><subject>Plants, Genetically Modified - cytology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - physiology</subject><subject>Populus - cytology</subject><subject>Populus - genetics</subject><subject>Populus - physiology</subject><subject>Populus maximowiczii</subject><subject>Populus nigra</subject><subject>potassium nitrate</subject><subject>protein synthesis</subject><subject>putrescine</subject><subject>Putrescine - biosynthesis</subject><subject>transgenic plants</subject><subject>Trees - cytology</subject><subject>Trees - genetics</subject><subject>Trees - physiology</subject><subject>wild relatives</subject><issn>0829-318X</issn><issn>1758-4469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkd9qFDEchYModrv6AN5orrybbf7P5FKKWqGgoAXvQibzm21kJhmTTGVfxyc1293iVQg53xcOB6E3lOwo0fyqJIDl_pCvmNjJnZT0GdrQVnaNEEo_RxvSMd1w2v28QJc5_yKEyq7TL9EFFVorJfUG_f1WeR-nuPfOTjhBXmLIkHEc8R8_DbgcFsA2DHhZ63fZ-QBNfIC0pDis9bbHJdmQ9xC8ww6m6RFd4jLZhEvEDzZ5W3yVYh9wuQc8xjQ_Gl0MDkLFj89HKviSYjU9JWcY_Dq_Qi9GO2V4fT636O7Txx_XN83t189frj_cNo4rURpFZd8JoAqIZXZk3TgMHXF9S4gShPeaKS4t7ZUUjlJBNGXKtY63lDDoec-36P3JW6v9XiEXM_t8bGQDxDWbliqtWbVsET0FXYo5JxjNkvxs08FQYo7DmKdhDBNGmjpMZd6e5Wtfa_0nzkvUwLtTYLTR2H3y2dx9Z4RyQrSQjLf8H6JXmWc</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Minocha, R</creator><creator>Lee, J.S</creator><creator>Long, S</creator><creator>Bhatnagar, P</creator><creator>Minocha, S.C</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium</title><author>Minocha, R ; Lee, J.S ; Long, S ; Bhatnagar, P ; Minocha, S.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-615b84e16e0a2af28fdd80cb7006403b92635a1b654c11409126c7c37102eb3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>ammonium nitrate</topic><topic>biochemical pathways</topic><topic>biosynthesis</topic><topic>cell membranes</topic><topic>cell suspension culture</topic><topic>Cells, Cultured</topic><topic>chemical concentration</topic><topic>chemical reactions</topic><topic>Culture Media</topic><topic>gene expression</topic><topic>genes</topic><topic>hybrids</topic><topic>metabolism</topic><topic>Nitrogen - metabolism</topic><topic>nitrogen content</topic><topic>nutrient requirements</topic><topic>permeability</topic><topic>Plants, Genetically Modified - cytology</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - physiology</topic><topic>Populus - cytology</topic><topic>Populus - genetics</topic><topic>Populus - physiology</topic><topic>Populus maximowiczii</topic><topic>Populus nigra</topic><topic>potassium nitrate</topic><topic>protein synthesis</topic><topic>putrescine</topic><topic>Putrescine - biosynthesis</topic><topic>transgenic plants</topic><topic>Trees - cytology</topic><topic>Trees - genetics</topic><topic>Trees - physiology</topic><topic>wild relatives</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minocha, R</creatorcontrib><creatorcontrib>Lee, J.S</creatorcontrib><creatorcontrib>Long, S</creatorcontrib><creatorcontrib>Bhatnagar, P</creatorcontrib><creatorcontrib>Minocha, S.C</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Tree physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minocha, R</au><au>Lee, J.S</au><au>Long, S</au><au>Bhatnagar, P</au><au>Minocha, S.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium</atitle><jtitle>Tree physiology</jtitle><addtitle>Tree Physiol</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>24</volume><issue>5</issue><spage>551</spage><epage>560</epage><pages>551-560</pages><issn>0829-318X</issn><eissn>1758-4469</eissn><abstract>We determined: (a) the physiological consequences of overproduction of putrescine in transgenic poplar (Populus nigra x maximoviczii) cells expressing an ornithine decarboxylase transgene; and (b) effects of variation in nitrogen (N) concentration of the medium on cellular polyamine concentration in transgenic and non-transgenic cells. Cells grown in the presence of supplemental (to the normal concentrations of N sources in the growth medium) and reduced amounts of NH4NO3 and KNO3 were used to study effects on membrane permeability, mitochondrial respiratory activity, protein accumulation, growth rates and changes in cellular polyamine concentration. The N concentration of the MS medium was not a limiting factor for continued overproduction of putrescine in transgenic cells. However, continued supplies of NH4+ and NO3- were required to maintain homeostatic amounts of putrescine in both cell lines. The presence of high amounts of putrescine in transgenic cells had significant effects on the physiological parameters measured. Compared with non-transgenic cells, transgenic cells had greater plasma membrane permeability, less tolerance to NH4NO3, more tolerance to KNO3, and accumulated higher amounts of soluble protein.</abstract><cop>Canada</cop><pmid>14996659</pmid><doi>10.1093/treephys/24.5.551</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Tree physiology, 2004-05, Vol.24 (5), p.551-560 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | ammonium nitrate biochemical pathways biosynthesis cell membranes cell suspension culture Cells, Cultured chemical concentration chemical reactions Culture Media gene expression genes hybrids metabolism Nitrogen - metabolism nitrogen content nutrient requirements permeability Plants, Genetically Modified - cytology Plants, Genetically Modified - genetics Plants, Genetically Modified - physiology Populus - cytology Populus - genetics Populus - physiology Populus maximowiczii Populus nigra potassium nitrate protein synthesis putrescine Putrescine - biosynthesis transgenic plants Trees - cytology Trees - genetics Trees - physiology wild relatives |
| title | Physiological responses of wild type and putrescine-overproducing transgenic cells of poplar to variations in the form and concentration of nitrogen in the medium |
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