Dynamic perifusion to maintain and assess isolated pancreatic islets

Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability w...

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Veröffentlicht in:	Diabetes technology & therapeutics 2002, Vol.4 (1), p.67-76
Hauptverfasser:	Sweet, Ian R, Cook, Daniel L, Wiseman, Robert W, Greenbaum, Carla J, Lernmark, Ake, Matsumoto, Shinichi, Teague, Jeanette C, Krohn, Kenneth A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Line Cells, Cultured Glucose - pharmacology Humans Insulin - metabolism Insulin Secretion Islets of Langerhans - cytology Islets of Langerhans - drug effects Islets of Langerhans - metabolism Islets of Langerhans - physiology Kinetics Macaca nemestrina Oxygen Consumption Perfusion - methods Time Factors
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