Molecular and Biochemical Characterization of a Cold-Regulated Phosphoethanolamine N-Methyltransferase from Wheat

A cDNA that encodes a methyltransferase (MT) was cloned from a cold-acclimated wheat (Triticum aestivum) cDNA library. Molecular analysis indicated that the enzyme WPEAMT (wheat phosphoethanolamine [P-EA] MT) is a bipartite protein with two separate sets of S-adenosyl-L-Met-binding domains, one clos...

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Veröffentlicht in:	Plant physiology (Bethesda) 2002-05, Vol.129 (1), p.363-373
Hauptverfasser:	Jean-Benoit Frenette Charron, Ghislain Breton, Danyluk, Jean, Ingrid Muzac, Ibrahim, Ragai K., Sarhan, Fathey
Format:	Artikel
Sprache:	eng
Schlagworte:	Abscisic Acid - pharmacology Acclimatization Acclimatization - genetics Acclimatization - physiology Adaptation to environment and cultivation conditions Agronomy. Soil science and plant productions Amino Acid Sequence Base Sequence Biological and medical sciences Catalytic Domain - genetics Cloning, Molecular Cold Temperature DNA, Complementary - chemistry DNA, Complementary - genetics Environmental Stress and Adaptation Enzymatic activity Enzymes Escherichia coli - genetics Freezing Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - drug effects Genetics and breeding of economic plants glycine betaine Lipids Methylation Methyltransferases - genetics Methyltransferases - isolation & purification Methyltransferases - metabolism Molecular Sequence Data phosphocholine Physical agents Plant physiology and development Plant Proteins - genetics Plant Proteins - isolation & purification Plant Proteins - metabolism Plants Protein synthesis Proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Alignment Sequence Analysis, DNA Sodium Chloride - pharmacology Spinach Substrate specificity Triticum - enzymology Triticum - genetics Triticum aestivum Up-Regulation - drug effects Varietal selection. Specialized plant breeding, plant breeding aims Vegetative apparatus, growth and morphogenesis. Senescence Water - pharmacology Wheat Wpeamt gene
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