Spontaneous outgrowth of EBV-transformed B-cells reflects EBV-specific immunity in vivo; a useful tool in the follow-up of EBV-driven immunoproliferative disorders in allograft recipients
During immunosuppressive medication, Epstein-Barr virus (EBV) infection is associated with a risk of developing posttransplant lymphoproliferative disease (PTLD). The appropriateness of a spontaneous EBV B-cell transformation (SET) assay as a monitor of EBV-specific immunity was evaluated to investi...
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| Veröffentlicht in: | Transplant international 2004-02, Vol.17 (2), p.89-96 |
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| creator | VOSSEN, Mireille T. M GENT, Mi-Ran KUIJPERS, Taco W DAVIN, Jean-Claude BAARS, Paul A DILLEN, Pauline M. E. Wertheim-Van WEEL, Jan F. L ROOS, Marijke T. L VAN BAARLE, Debbie GROOTHOFF, Jaap VAN LIER, René A. W |
| description | During immunosuppressive medication, Epstein-Barr virus (EBV) infection is associated with a risk of developing posttransplant lymphoproliferative disease (PTLD). The appropriateness of a spontaneous EBV B-cell transformation (SET) assay as a monitor of EBV-specific immunity was evaluated to investigate if it safely allows reducing immunosuppressive medication, thereby decreasing the risk of developing PTLD. PBMC were isolated longitudinally from 20 pediatric renal allograft recipients treated with prednisone and cyclosporine combined with either azathioprine or mycophenolate mofetil. Most significantly, EBV-peptide-specific CD8+ T cells were detectable in the blood of patients with negative SET assays, coinciding with significantly lower EBV loads, whereas these cells were less frequent in the blood of patients with positive SET assays. Reducing the levels of immunosuppression resulted in normalization of the SET assays. Therefore, the SET assay is a reflection of the interaction between viral replication, transformation of B cells, and EBV-specific immunity in vivo and hence a valuable screening test for EBV-driven lymphoproliferative phenomena in allograft recipients. |
| doi_str_mv | 10.1007/s00147-003-0665-4 |
| format | Article |
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M ; GENT, Mi-Ran ; KUIJPERS, Taco W ; DAVIN, Jean-Claude ; BAARS, Paul A ; DILLEN, Pauline M. E. Wertheim-Van ; WEEL, Jan F. L ; ROOS, Marijke T. L ; VAN BAARLE, Debbie ; GROOTHOFF, Jaap ; VAN LIER, René A. W</creator><creatorcontrib>VOSSEN, Mireille T. M ; GENT, Mi-Ran ; KUIJPERS, Taco W ; DAVIN, Jean-Claude ; BAARS, Paul A ; DILLEN, Pauline M. E. Wertheim-Van ; WEEL, Jan F. L ; ROOS, Marijke T. L ; VAN BAARLE, Debbie ; GROOTHOFF, Jaap ; VAN LIER, René A. W</creatorcontrib><description>During immunosuppressive medication, Epstein-Barr virus (EBV) infection is associated with a risk of developing posttransplant lymphoproliferative disease (PTLD). The appropriateness of a spontaneous EBV B-cell transformation (SET) assay as a monitor of EBV-specific immunity was evaluated to investigate if it safely allows reducing immunosuppressive medication, thereby decreasing the risk of developing PTLD. PBMC were isolated longitudinally from 20 pediatric renal allograft recipients treated with prednisone and cyclosporine combined with either azathioprine or mycophenolate mofetil. Most significantly, EBV-peptide-specific CD8+ T cells were detectable in the blood of patients with negative SET assays, coinciding with significantly lower EBV loads, whereas these cells were less frequent in the blood of patients with positive SET assays. Reducing the levels of immunosuppression resulted in normalization of the SET assays. Therefore, the SET assay is a reflection of the interaction between viral replication, transformation of B cells, and EBV-specific immunity in vivo and hence a valuable screening test for EBV-driven lymphoproliferative phenomena in allograft recipients.</description><identifier>ISSN: 0934-0874</identifier><identifier>EISSN: 1432-2277</identifier><identifier>DOI: 10.1007/s00147-003-0665-4</identifier><identifier>PMID: 14652716</identifier><language>eng</language><publisher>Oxford: Blackwell Publishing</publisher><subject>Antigens, CD - blood ; Biological and medical sciences ; CD8-Positive T-Lymphocytes - immunology ; Cell Transformation, Viral - immunology ; Follow-Up Studies ; General aspects ; Hematologic and hematopoietic diseases ; Herpesvirus 4, Human - immunology ; Humans ; Immunity ; Immunoproliferative Disorders - virology ; Kidney Transplantation - immunology ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Lymphocyte Count ; Lymphocyte Subsets - immunology ; Medical sciences ; Pharmacology. Drug treatments ; Postoperative Complications - immunology ; Postoperative Complications - virology ; Surgery (general aspects). Transplantations, organ and tissue grafts. 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M</creatorcontrib><creatorcontrib>GENT, Mi-Ran</creatorcontrib><creatorcontrib>KUIJPERS, Taco W</creatorcontrib><creatorcontrib>DAVIN, Jean-Claude</creatorcontrib><creatorcontrib>BAARS, Paul A</creatorcontrib><creatorcontrib>DILLEN, Pauline M. E. Wertheim-Van</creatorcontrib><creatorcontrib>WEEL, Jan F. L</creatorcontrib><creatorcontrib>ROOS, Marijke T. L</creatorcontrib><creatorcontrib>VAN BAARLE, Debbie</creatorcontrib><creatorcontrib>GROOTHOFF, Jaap</creatorcontrib><creatorcontrib>VAN LIER, René A. W</creatorcontrib><title>Spontaneous outgrowth of EBV-transformed B-cells reflects EBV-specific immunity in vivo; a useful tool in the follow-up of EBV-driven immunoproliferative disorders in allograft recipients</title><title>Transplant international</title><addtitle>Transpl Int</addtitle><description>During immunosuppressive medication, Epstein-Barr virus (EBV) infection is associated with a risk of developing posttransplant lymphoproliferative disease (PTLD). The appropriateness of a spontaneous EBV B-cell transformation (SET) assay as a monitor of EBV-specific immunity was evaluated to investigate if it safely allows reducing immunosuppressive medication, thereby decreasing the risk of developing PTLD. PBMC were isolated longitudinally from 20 pediatric renal allograft recipients treated with prednisone and cyclosporine combined with either azathioprine or mycophenolate mofetil. Most significantly, EBV-peptide-specific CD8+ T cells were detectable in the blood of patients with negative SET assays, coinciding with significantly lower EBV loads, whereas these cells were less frequent in the blood of patients with positive SET assays. Reducing the levels of immunosuppression resulted in normalization of the SET assays. Therefore, the SET assay is a reflection of the interaction between viral replication, transformation of B cells, and EBV-specific immunity in vivo and hence a valuable screening test for EBV-driven lymphoproliferative phenomena in allograft recipients.</description><subject>Antigens, CD - blood</subject><subject>Biological and medical sciences</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cell Transformation, Viral - immunology</subject><subject>Follow-Up Studies</subject><subject>General aspects</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Herpesvirus 4, Human - immunology</subject><subject>Humans</subject><subject>Immunity</subject><subject>Immunoproliferative Disorders - virology</subject><subject>Kidney Transplantation - immunology</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Lymphocyte Count</subject><subject>Lymphocyte Subsets - immunology</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Postoperative Complications - immunology</subject><subject>Postoperative Complications - virology</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. 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Drug treatments</topic><topic>Postoperative Complications - immunology</topic><topic>Postoperative Complications - virology</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Transplantation, Homologous - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VOSSEN, Mireille T. M</creatorcontrib><creatorcontrib>GENT, Mi-Ran</creatorcontrib><creatorcontrib>KUIJPERS, Taco W</creatorcontrib><creatorcontrib>DAVIN, Jean-Claude</creatorcontrib><creatorcontrib>BAARS, Paul A</creatorcontrib><creatorcontrib>DILLEN, Pauline M. E. Wertheim-Van</creatorcontrib><creatorcontrib>WEEL, Jan F. L</creatorcontrib><creatorcontrib>ROOS, Marijke T. L</creatorcontrib><creatorcontrib>VAN BAARLE, Debbie</creatorcontrib><creatorcontrib>GROOTHOFF, Jaap</creatorcontrib><creatorcontrib>VAN LIER, René A. 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M</au><au>GENT, Mi-Ran</au><au>KUIJPERS, Taco W</au><au>DAVIN, Jean-Claude</au><au>BAARS, Paul A</au><au>DILLEN, Pauline M. E. Wertheim-Van</au><au>WEEL, Jan F. L</au><au>ROOS, Marijke T. L</au><au>VAN BAARLE, Debbie</au><au>GROOTHOFF, Jaap</au><au>VAN LIER, René A. W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spontaneous outgrowth of EBV-transformed B-cells reflects EBV-specific immunity in vivo; a useful tool in the follow-up of EBV-driven immunoproliferative disorders in allograft recipients</atitle><jtitle>Transplant international</jtitle><addtitle>Transpl Int</addtitle><date>2004-02-01</date><risdate>2004</risdate><volume>17</volume><issue>2</issue><spage>89</spage><epage>96</epage><pages>89-96</pages><issn>0934-0874</issn><eissn>1432-2277</eissn><abstract>During immunosuppressive medication, Epstein-Barr virus (EBV) infection is associated with a risk of developing posttransplant lymphoproliferative disease (PTLD). The appropriateness of a spontaneous EBV B-cell transformation (SET) assay as a monitor of EBV-specific immunity was evaluated to investigate if it safely allows reducing immunosuppressive medication, thereby decreasing the risk of developing PTLD. PBMC were isolated longitudinally from 20 pediatric renal allograft recipients treated with prednisone and cyclosporine combined with either azathioprine or mycophenolate mofetil. Most significantly, EBV-peptide-specific CD8+ T cells were detectable in the blood of patients with negative SET assays, coinciding with significantly lower EBV loads, whereas these cells were less frequent in the blood of patients with positive SET assays. Reducing the levels of immunosuppression resulted in normalization of the SET assays. 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| ispartof | Transplant international, 2004-02, Vol.17 (2), p.89-96 |
| issn | 0934-0874 1432-2277 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Antigens, CD - blood Biological and medical sciences CD8-Positive T-Lymphocytes - immunology Cell Transformation, Viral - immunology Follow-Up Studies General aspects Hematologic and hematopoietic diseases Herpesvirus 4, Human - immunology Humans Immunity Immunoproliferative Disorders - virology Kidney Transplantation - immunology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphocyte Count Lymphocyte Subsets - immunology Medical sciences Pharmacology. Drug treatments Postoperative Complications - immunology Postoperative Complications - virology Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Transplantation, Homologous - immunology |
| title | Spontaneous outgrowth of EBV-transformed B-cells reflects EBV-specific immunity in vivo; a useful tool in the follow-up of EBV-driven immunoproliferative disorders in allograft recipients |
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