Pressure simulation of orthodontic force in osteoblasts: a pilot study

Structured Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading. Design – Using a stringent pulse‐chase protocol human osteoblasts were expose...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Clinical orthodontics and research 2004-02, Vol.7 (1), p.3-9
Hauptverfasser: Baumert, U., Golan, I., Becker, B., Hrala, B.P., Redlich, M., Roos, H.A., Palmon, A., Reichenberg, E., Müßig, D.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 9
container_issue 1
container_start_page 3
container_title Clinical orthodontics and research
container_volume 7
creator Baumert, U.
Golan, I.
Becker, B.
Hrala, B.P.
Redlich, M.
Roos, H.A.
Palmon, A.
Reichenberg, E.
Müßig, D.
description Structured Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading. Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application. Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression. Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup.
doi_str_mv 10.1046/j.1601-6335.2003.00270.x
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71689758</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19728914</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4140-c6e9eee63f359fac9f7a79b6e3547c2241931e15a470db06d6e14297986e3aa13</originalsourceid><addsrcrecordid>eNqNkMtOwzAQRS0E4v0LyCt2CX7FjhEbVPEUAoSKurTcZCJc0rrYiWj_HpdWsISVR55zZzQHIUxJTomQZ5OcSkIzyXmRM0J4TghTJF9sof1NQ_Dtn5oXe-ggxkmCCGNyF-1RoUutRLmPrp8DxNgHwNFN-9Z2zs-wb7AP3Zuv_axzFW58qAC79B878OPWxi6eY4vnrvUdjl1fL4_QTmPbCMeb9xC9Xl8NB7fZw9PN3eDyIasEFSSrJGgAkLzhhW5spRtllR5L4IVQFWOCak6BFlYoUo-JrCVQwbTSZUKspfwQna7nzoP_6CF2ZupiBW1rZ-D7aBSV6bCi_BOkWrFSU5HAcg1WwccYoDHz4KY2LA0lZiXbTMzKo1l5NCvZ5lu2WaToyWZHP55C_Rvc2E3AxRr4dC0s_z3YPA1eUpHi2TrukvfFT9yGdyMVV4UZPd6YUg_v-Ug-Gsa_AMgim_o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19728914</pqid></control><display><type>article</type><title>Pressure simulation of orthodontic force in osteoblasts: a pilot study</title><source>MEDLINE</source><source>Wiley Online Library Journals</source><creator>Baumert, U. ; Golan, I. ; Becker, B. ; Hrala, B.P. ; Redlich, M. ; Roos, H.A. ; Palmon, A. ; Reichenberg, E. ; Müßig, D.</creator><creatorcontrib>Baumert, U. ; Golan, I. ; Becker, B. ; Hrala, B.P. ; Redlich, M. ; Roos, H.A. ; Palmon, A. ; Reichenberg, E. ; Müßig, D.</creatorcontrib><description>Structured Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading. Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application. Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression. Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup.</description><identifier>ISSN: 1601-6335</identifier><identifier>ISSN: 1397-5927</identifier><identifier>EISSN: 1601-6343</identifier><identifier>DOI: 10.1046/j.1601-6335.2003.00270.x</identifier><identifier>PMID: 14989748</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishers</publisher><subject>Cells, Cultured ; Centrifugation ; Core Binding Factor Alpha 1 Subunit ; Dental Stress Analysis ; Dentistry ; Humans ; mechanical load simulation ; Neoplasm Proteins - biosynthesis ; orthodontic tooth movement ; osteoblasts ; Osteoblasts - metabolism ; Osteoblasts - physiology ; Pilot Projects ; Pressure ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - analysis ; RUNX2 ; Tooth Movement Techniques ; Transcription Factors - biosynthesis</subject><ispartof>Clinical orthodontics and research, 2004-02, Vol.7 (1), p.3-9</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4140-c6e9eee63f359fac9f7a79b6e3547c2241931e15a470db06d6e14297986e3aa13</citedby><cites>FETCH-LOGICAL-c4140-c6e9eee63f359fac9f7a79b6e3547c2241931e15a470db06d6e14297986e3aa13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1601-6335.2003.00270.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1601-6335.2003.00270.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14989748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baumert, U.</creatorcontrib><creatorcontrib>Golan, I.</creatorcontrib><creatorcontrib>Becker, B.</creatorcontrib><creatorcontrib>Hrala, B.P.</creatorcontrib><creatorcontrib>Redlich, M.</creatorcontrib><creatorcontrib>Roos, H.A.</creatorcontrib><creatorcontrib>Palmon, A.</creatorcontrib><creatorcontrib>Reichenberg, E.</creatorcontrib><creatorcontrib>Müßig, D.</creatorcontrib><title>Pressure simulation of orthodontic force in osteoblasts: a pilot study</title><title>Clinical orthodontics and research</title><addtitle>Orthod Craniofac Res</addtitle><description>Structured Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading. Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application. Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression. Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup.</description><subject>Cells, Cultured</subject><subject>Centrifugation</subject><subject>Core Binding Factor Alpha 1 Subunit</subject><subject>Dental Stress Analysis</subject><subject>Dentistry</subject><subject>Humans</subject><subject>mechanical load simulation</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>orthodontic tooth movement</subject><subject>osteoblasts</subject><subject>Osteoblasts - metabolism</subject><subject>Osteoblasts - physiology</subject><subject>Pilot Projects</subject><subject>Pressure</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - analysis</subject><subject>RUNX2</subject><subject>Tooth Movement Techniques</subject><subject>Transcription Factors - biosynthesis</subject><issn>1601-6335</issn><issn>1397-5927</issn><issn>1601-6343</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAQRS0E4v0LyCt2CX7FjhEbVPEUAoSKurTcZCJc0rrYiWj_HpdWsISVR55zZzQHIUxJTomQZ5OcSkIzyXmRM0J4TghTJF9sof1NQ_Dtn5oXe-ggxkmCCGNyF-1RoUutRLmPrp8DxNgHwNFN-9Z2zs-wb7AP3Zuv_axzFW58qAC79B878OPWxi6eY4vnrvUdjl1fL4_QTmPbCMeb9xC9Xl8NB7fZw9PN3eDyIasEFSSrJGgAkLzhhW5spRtllR5L4IVQFWOCak6BFlYoUo-JrCVQwbTSZUKspfwQna7nzoP_6CF2ZupiBW1rZ-D7aBSV6bCi_BOkWrFSU5HAcg1WwccYoDHz4KY2LA0lZiXbTMzKo1l5NCvZ5lu2WaToyWZHP55C_Rvc2E3AxRr4dC0s_z3YPA1eUpHi2TrukvfFT9yGdyMVV4UZPd6YUg_v-Ug-Gsa_AMgim_o</recordid><startdate>200402</startdate><enddate>200402</enddate><creator>Baumert, U.</creator><creator>Golan, I.</creator><creator>Becker, B.</creator><creator>Hrala, B.P.</creator><creator>Redlich, M.</creator><creator>Roos, H.A.</creator><creator>Palmon, A.</creator><creator>Reichenberg, E.</creator><creator>Müßig, D.</creator><general>Blackwell Publishers</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200402</creationdate><title>Pressure simulation of orthodontic force in osteoblasts: a pilot study</title><author>Baumert, U. ; Golan, I. ; Becker, B. ; Hrala, B.P. ; Redlich, M. ; Roos, H.A. ; Palmon, A. ; Reichenberg, E. ; Müßig, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4140-c6e9eee63f359fac9f7a79b6e3547c2241931e15a470db06d6e14297986e3aa13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Cells, Cultured</topic><topic>Centrifugation</topic><topic>Core Binding Factor Alpha 1 Subunit</topic><topic>Dental Stress Analysis</topic><topic>Dentistry</topic><topic>Humans</topic><topic>mechanical load simulation</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>orthodontic tooth movement</topic><topic>osteoblasts</topic><topic>Osteoblasts - metabolism</topic><topic>Osteoblasts - physiology</topic><topic>Pilot Projects</topic><topic>Pressure</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - analysis</topic><topic>RUNX2</topic><topic>Tooth Movement Techniques</topic><topic>Transcription Factors - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baumert, U.</creatorcontrib><creatorcontrib>Golan, I.</creatorcontrib><creatorcontrib>Becker, B.</creatorcontrib><creatorcontrib>Hrala, B.P.</creatorcontrib><creatorcontrib>Redlich, M.</creatorcontrib><creatorcontrib>Roos, H.A.</creatorcontrib><creatorcontrib>Palmon, A.</creatorcontrib><creatorcontrib>Reichenberg, E.</creatorcontrib><creatorcontrib>Müßig, D.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical orthodontics and research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baumert, U.</au><au>Golan, I.</au><au>Becker, B.</au><au>Hrala, B.P.</au><au>Redlich, M.</au><au>Roos, H.A.</au><au>Palmon, A.</au><au>Reichenberg, E.</au><au>Müßig, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pressure simulation of orthodontic force in osteoblasts: a pilot study</atitle><jtitle>Clinical orthodontics and research</jtitle><addtitle>Orthod Craniofac Res</addtitle><date>2004-02</date><risdate>2004</risdate><volume>7</volume><issue>1</issue><spage>3</spage><epage>9</epage><pages>3-9</pages><issn>1601-6335</issn><issn>1397-5927</issn><eissn>1601-6343</eissn><abstract>Structured Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading. Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application. Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression. Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishers</pub><pmid>14989748</pmid><doi>10.1046/j.1601-6335.2003.00270.x</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1601-6335
ispartof Clinical orthodontics and research, 2004-02, Vol.7 (1), p.3-9
issn 1601-6335
1397-5927
1601-6343
language eng
recordid cdi_proquest_miscellaneous_71689758
source MEDLINE; Wiley Online Library Journals
subjects Cells, Cultured
Centrifugation
Core Binding Factor Alpha 1 Subunit
Dental Stress Analysis
Dentistry
Humans
mechanical load simulation
Neoplasm Proteins - biosynthesis
orthodontic tooth movement
osteoblasts
Osteoblasts - metabolism
Osteoblasts - physiology
Pilot Projects
Pressure
Reverse Transcriptase Polymerase Chain Reaction
RNA - analysis
RUNX2
Tooth Movement Techniques
Transcription Factors - biosynthesis
title Pressure simulation of orthodontic force in osteoblasts: a pilot study
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T14%3A07%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pressure%20simulation%20of%20orthodontic%20force%20in%20osteoblasts:%20a%20pilot%20study&rft.jtitle=Clinical%20orthodontics%20and%20research&rft.au=Baumert,%20U.&rft.date=2004-02&rft.volume=7&rft.issue=1&rft.spage=3&rft.epage=9&rft.pages=3-9&rft.issn=1601-6335&rft.eissn=1601-6343&rft_id=info:doi/10.1046/j.1601-6335.2003.00270.x&rft_dat=%3Cproquest_cross%3E19728914%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19728914&rft_id=info:pmid/14989748&rfr_iscdi=true