Pressure simulation of orthodontic force in osteoblasts: a pilot study
Structured Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading. Design – Using a stringent pulse‐chase protocol human osteoblasts were expose...
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creator | Baumert, U. Golan, I. Becker, B. Hrala, B.P. Redlich, M. Roos, H.A. Palmon, A. Reichenberg, E. Müßig, D. |
description | Structured
Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D
Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading.
Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application.
Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression.
Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup. |
doi_str_mv | 10.1046/j.1601-6335.2003.00270.x |
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Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D
Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading.
Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application.
Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression.
Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup.</description><identifier>ISSN: 1601-6335</identifier><identifier>ISSN: 1397-5927</identifier><identifier>EISSN: 1601-6343</identifier><identifier>DOI: 10.1046/j.1601-6335.2003.00270.x</identifier><identifier>PMID: 14989748</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishers</publisher><subject>Cells, Cultured ; Centrifugation ; Core Binding Factor Alpha 1 Subunit ; Dental Stress Analysis ; Dentistry ; Humans ; mechanical load simulation ; Neoplasm Proteins - biosynthesis ; orthodontic tooth movement ; osteoblasts ; Osteoblasts - metabolism ; Osteoblasts - physiology ; Pilot Projects ; Pressure ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - analysis ; RUNX2 ; Tooth Movement Techniques ; Transcription Factors - biosynthesis</subject><ispartof>Clinical orthodontics and research, 2004-02, Vol.7 (1), p.3-9</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4140-c6e9eee63f359fac9f7a79b6e3547c2241931e15a470db06d6e14297986e3aa13</citedby><cites>FETCH-LOGICAL-c4140-c6e9eee63f359fac9f7a79b6e3547c2241931e15a470db06d6e14297986e3aa13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1601-6335.2003.00270.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1601-6335.2003.00270.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14989748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baumert, U.</creatorcontrib><creatorcontrib>Golan, I.</creatorcontrib><creatorcontrib>Becker, B.</creatorcontrib><creatorcontrib>Hrala, B.P.</creatorcontrib><creatorcontrib>Redlich, M.</creatorcontrib><creatorcontrib>Roos, H.A.</creatorcontrib><creatorcontrib>Palmon, A.</creatorcontrib><creatorcontrib>Reichenberg, E.</creatorcontrib><creatorcontrib>Müßig, D.</creatorcontrib><title>Pressure simulation of orthodontic force in osteoblasts: a pilot study</title><title>Clinical orthodontics and research</title><addtitle>Orthod Craniofac Res</addtitle><description>Structured
Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D
Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading.
Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application.
Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression.
Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup.</description><subject>Cells, Cultured</subject><subject>Centrifugation</subject><subject>Core Binding Factor Alpha 1 Subunit</subject><subject>Dental Stress Analysis</subject><subject>Dentistry</subject><subject>Humans</subject><subject>mechanical load simulation</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>orthodontic tooth movement</subject><subject>osteoblasts</subject><subject>Osteoblasts - metabolism</subject><subject>Osteoblasts - physiology</subject><subject>Pilot Projects</subject><subject>Pressure</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - analysis</subject><subject>RUNX2</subject><subject>Tooth Movement Techniques</subject><subject>Transcription Factors - biosynthesis</subject><issn>1601-6335</issn><issn>1397-5927</issn><issn>1601-6343</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAQRS0E4v0LyCt2CX7FjhEbVPEUAoSKurTcZCJc0rrYiWj_HpdWsISVR55zZzQHIUxJTomQZ5OcSkIzyXmRM0J4TghTJF9sof1NQ_Dtn5oXe-ggxkmCCGNyF-1RoUutRLmPrp8DxNgHwNFN-9Z2zs-wb7AP3Zuv_axzFW58qAC79B878OPWxi6eY4vnrvUdjl1fL4_QTmPbCMeb9xC9Xl8NB7fZw9PN3eDyIasEFSSrJGgAkLzhhW5spRtllR5L4IVQFWOCak6BFlYoUo-JrCVQwbTSZUKspfwQna7nzoP_6CF2ZupiBW1rZ-D7aBSV6bCi_BOkWrFSU5HAcg1WwccYoDHz4KY2LA0lZiXbTMzKo1l5NCvZ5lu2WaToyWZHP55C_Rvc2E3AxRr4dC0s_z3YPA1eUpHi2TrukvfFT9yGdyMVV4UZPd6YUg_v-Ug-Gsa_AMgim_o</recordid><startdate>200402</startdate><enddate>200402</enddate><creator>Baumert, U.</creator><creator>Golan, I.</creator><creator>Becker, B.</creator><creator>Hrala, B.P.</creator><creator>Redlich, M.</creator><creator>Roos, H.A.</creator><creator>Palmon, A.</creator><creator>Reichenberg, E.</creator><creator>Müßig, D.</creator><general>Blackwell Publishers</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200402</creationdate><title>Pressure simulation of orthodontic force in osteoblasts: a pilot study</title><author>Baumert, U. ; Golan, I. ; Becker, B. ; Hrala, B.P. ; Redlich, M. ; Roos, H.A. ; Palmon, A. ; Reichenberg, E. ; Müßig, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4140-c6e9eee63f359fac9f7a79b6e3547c2241931e15a470db06d6e14297986e3aa13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Cells, Cultured</topic><topic>Centrifugation</topic><topic>Core Binding Factor Alpha 1 Subunit</topic><topic>Dental Stress Analysis</topic><topic>Dentistry</topic><topic>Humans</topic><topic>mechanical load simulation</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>orthodontic tooth movement</topic><topic>osteoblasts</topic><topic>Osteoblasts - metabolism</topic><topic>Osteoblasts - physiology</topic><topic>Pilot Projects</topic><topic>Pressure</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - analysis</topic><topic>RUNX2</topic><topic>Tooth Movement Techniques</topic><topic>Transcription Factors - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baumert, U.</creatorcontrib><creatorcontrib>Golan, I.</creatorcontrib><creatorcontrib>Becker, B.</creatorcontrib><creatorcontrib>Hrala, B.P.</creatorcontrib><creatorcontrib>Redlich, M.</creatorcontrib><creatorcontrib>Roos, H.A.</creatorcontrib><creatorcontrib>Palmon, A.</creatorcontrib><creatorcontrib>Reichenberg, E.</creatorcontrib><creatorcontrib>Müßig, D.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical orthodontics and research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baumert, U.</au><au>Golan, I.</au><au>Becker, B.</au><au>Hrala, B.P.</au><au>Redlich, M.</au><au>Roos, H.A.</au><au>Palmon, A.</au><au>Reichenberg, E.</au><au>Müßig, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pressure simulation of orthodontic force in osteoblasts: a pilot study</atitle><jtitle>Clinical orthodontics and research</jtitle><addtitle>Orthod Craniofac Res</addtitle><date>2004-02</date><risdate>2004</risdate><volume>7</volume><issue>1</issue><spage>3</spage><epage>9</epage><pages>3-9</pages><issn>1601-6335</issn><issn>1397-5927</issn><eissn>1601-6343</eissn><abstract>Structured
Authors – Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Reichenberg E, Palmon A, Müßig D
Objectives – To elucidate the RUNX2 gene expression induction in human osteoblasts after mechanical loading.
Design – Using a stringent pulse‐chase protocol human osteoblasts were exposed to centrifugal pressure force for 30 and 90 min. Untreated control cells were processed in parallel. Before, and at defined times after centrifugation, total RNA was isolated. RUNX2 gene expression was measured using real‐time quantitative reverse transcriptase polymerase chain reaction. The stress/control ratio was used to illustrate possible stimulatory or diminishing effects of force application.
Results – Immediately after 30 min of force application the RUNX2 gene expression was induced by a factor of 1.7 ± 0.14 as compared with the negative control. This induction decreased rapidly and reached its pre‐load levels within 30 min. Longer force applications (up to 90 min) did not change the RUNX2 gene expression.
Conclusion – In mature osteoblasts centrifugal pressure force stimulates RUNX2 gene expression within a narrow time frame: loading of mature cells results in a temporary increase of RUNX2 expression and a fast downregulation back to its pre‐load expression level. With this pilot study the gene expression behavior after mechanical stimuli could be determined with a simple laboratory setup.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishers</pub><pmid>14989748</pmid><doi>10.1046/j.1601-6335.2003.00270.x</doi><tpages>7</tpages></addata></record> |
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subjects | Cells, Cultured Centrifugation Core Binding Factor Alpha 1 Subunit Dental Stress Analysis Dentistry Humans mechanical load simulation Neoplasm Proteins - biosynthesis orthodontic tooth movement osteoblasts Osteoblasts - metabolism Osteoblasts - physiology Pilot Projects Pressure Reverse Transcriptase Polymerase Chain Reaction RNA - analysis RUNX2 Tooth Movement Techniques Transcription Factors - biosynthesis |
title | Pressure simulation of orthodontic force in osteoblasts: a pilot study |
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