LC determination of flavonoids: separation of quercetin, luteolin and 3- O-methylquercetin in Achyrocline satureioides preparations
The pharmacological activities of the flavonoids show the interest in quantifying these constituents in phytopharmaceutical preparations, as well as in the validation of the analytical methodologies. LC methods have been reported to quantify isolated flavonoids or these compounds in complex biologic...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2002-05, Vol.28 (3), p.771-777 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | De Souza, K.C.B Schapoval, E.E.S Bassani, V.L |
| description | The pharmacological activities of the flavonoids show the interest in quantifying these constituents in phytopharmaceutical preparations, as well as in the validation of the analytical methodologies. LC methods have been reported to quantify isolated flavonoids or these compounds in complex biological matrices, such as herbal raw materials and extractive preparations. This work was designed, therefore, to develop an LC system to separate quercetin, luteolin and 3-
O-methylquercetin and to quantify them in extractive solutions from
Achyrocline satureioides. The main validation parameters of the method are also determined. The method showed linearity for quercetin and luteolin in the range 1–10 μg/ml. The aqueous and ethanol 80% extractive solutions showed linear response in the range 2.5–20 μl/ml and ethanol 40% extractive solution in the range 2.5–10 μl/ml. Precision and accuracy were determined for ethanol 80% extractive solution, in the concentration of 10 μl/ml. The LC method showed an excellent performance in separating the flavonoids quercetin, luteolin and 3-
O-methylquercetin in
A. satureioides extracts, since the presence of interference has been previously evaluated. |
| doi_str_mv | 10.1016/S0731-7085(01)00693-8 |
| format | Article |
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O-methylquercetin and to quantify them in extractive solutions from
Achyrocline satureioides. The main validation parameters of the method are also determined. The method showed linearity for quercetin and luteolin in the range 1–10 μg/ml. The aqueous and ethanol 80% extractive solutions showed linear response in the range 2.5–20 μl/ml and ethanol 40% extractive solution in the range 2.5–10 μl/ml. Precision and accuracy were determined for ethanol 80% extractive solution, in the concentration of 10 μl/ml. The LC method showed an excellent performance in separating the flavonoids quercetin, luteolin and 3-
O-methylquercetin in
A. satureioides extracts, since the presence of interference has been previously evaluated.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(01)00693-8</identifier><identifier>PMID: 12008157</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>3- O-Methylquercetin ; Achyrocline satureioides ; Analysis ; Biological and medical sciences ; Calibration ; Chromatography, Liquid ; Ethanol ; Flavonoids ; Flavonoids - analysis ; Flavonoids - isolation & purification ; General pharmacology ; Indicators and Reagents ; Luteolin ; Medical sciences ; Pharmacology. Drug treatments ; Plants, Medicinal - chemistry ; Quercetin ; Quercetin - analogs & derivatives ; Quercetin - analysis ; Quercetin - isolation & purification ; Reproducibility of Results ; Solutions ; Solvents ; Spectrophotometry, Ultraviolet ; Water</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2002-05, Vol.28 (3), p.771-777</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-3083b62297f111500f444a3b5e2e99f1f290653f006c35c40760c1e70b71ea8e3</citedby><cites>FETCH-LOGICAL-c391t-3083b62297f111500f444a3b5e2e99f1f290653f006c35c40760c1e70b71ea8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0731-7085(01)00693-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13682716$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12008157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Souza, K.C.B</creatorcontrib><creatorcontrib>Schapoval, E.E.S</creatorcontrib><creatorcontrib>Bassani, V.L</creatorcontrib><title>LC determination of flavonoids: separation of quercetin, luteolin and 3- O-methylquercetin in Achyrocline satureioides preparations</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>The pharmacological activities of the flavonoids show the interest in quantifying these constituents in phytopharmaceutical preparations, as well as in the validation of the analytical methodologies. LC methods have been reported to quantify isolated flavonoids or these compounds in complex biological matrices, such as herbal raw materials and extractive preparations. This work was designed, therefore, to develop an LC system to separate quercetin, luteolin and 3-
O-methylquercetin and to quantify them in extractive solutions from
Achyrocline satureioides. The main validation parameters of the method are also determined. The method showed linearity for quercetin and luteolin in the range 1–10 μg/ml. The aqueous and ethanol 80% extractive solutions showed linear response in the range 2.5–20 μl/ml and ethanol 40% extractive solution in the range 2.5–10 μl/ml. Precision and accuracy were determined for ethanol 80% extractive solution, in the concentration of 10 μl/ml. The LC method showed an excellent performance in separating the flavonoids quercetin, luteolin and 3-
O-methylquercetin in
A. satureioides extracts, since the presence of interference has been previously evaluated.</description><subject>3- O-Methylquercetin</subject><subject>Achyrocline satureioides</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chromatography, Liquid</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Flavonoids - analysis</subject><subject>Flavonoids - isolation & purification</subject><subject>General pharmacology</subject><subject>Indicators and Reagents</subject><subject>Luteolin</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Plants, Medicinal - chemistry</subject><subject>Quercetin</subject><subject>Quercetin - analogs & derivatives</subject><subject>Quercetin - analysis</subject><subject>Quercetin - isolation & purification</subject><subject>Reproducibility of Results</subject><subject>Solutions</subject><subject>Solvents</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Water</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo7uzqT1ByUVawtarT6XR7kWXwCwb2oIK3kElX2Eh3Z0zSC3P2j5vdGWaPQqAOeeqtl4exFwjvELB9_x2UwEpBJy8B3wC0vai6R2yFnRJV3Ta_HrPVCTlj5yn9BgCJffOUnWEN0KFUK_Z3s-YDZYqTn032YebBcTea2zAHP6QPPNHOxNPPn4Wipeznt3xcMoXRz9zMAxcVv64myjf78YTw8q7szT4GWzDiyeQlki-xlPgunnLTM_bEmTHR8-O8YD8_f_qx_lptrr98W19tKit6zJWATmzbuu6VQ0QJ4JqmMWIrqaa-d-jqHlopXHFhhbQNqBYskoKtQjIdiQv2-pC7i6G0TFlPPlkaRzNTWJJW2HayeCmgPIA2hpQiOb2LfjJxrxH0nX19b1_fqdWA-t6-7srey-OBZTvR8LB11F2AV0fAJGtGF81sfXrgRNvVpUXhPh44KjpuPUWdrKfZ0uAj2ayH4P9T5R_q5KKP</recordid><startdate>20020515</startdate><enddate>20020515</enddate><creator>De Souza, K.C.B</creator><creator>Schapoval, E.E.S</creator><creator>Bassani, V.L</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020515</creationdate><title>LC determination of flavonoids: separation of quercetin, luteolin and 3- O-methylquercetin in Achyrocline satureioides preparations</title><author>De Souza, K.C.B ; Schapoval, E.E.S ; Bassani, V.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-3083b62297f111500f444a3b5e2e99f1f290653f006c35c40760c1e70b71ea8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>3- O-Methylquercetin</topic><topic>Achyrocline satureioides</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chromatography, Liquid</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Flavonoids - analysis</topic><topic>Flavonoids - isolation & purification</topic><topic>General pharmacology</topic><topic>Indicators and Reagents</topic><topic>Luteolin</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Plants, Medicinal - chemistry</topic><topic>Quercetin</topic><topic>Quercetin - analogs & derivatives</topic><topic>Quercetin - analysis</topic><topic>Quercetin - isolation & purification</topic><topic>Reproducibility of Results</topic><topic>Solutions</topic><topic>Solvents</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Souza, K.C.B</creatorcontrib><creatorcontrib>Schapoval, E.E.S</creatorcontrib><creatorcontrib>Bassani, V.L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Souza, K.C.B</au><au>Schapoval, E.E.S</au><au>Bassani, V.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LC determination of flavonoids: separation of quercetin, luteolin and 3- O-methylquercetin in Achyrocline satureioides preparations</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2002-05-15</date><risdate>2002</risdate><volume>28</volume><issue>3</issue><spage>771</spage><epage>777</epage><pages>771-777</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>The pharmacological activities of the flavonoids show the interest in quantifying these constituents in phytopharmaceutical preparations, as well as in the validation of the analytical methodologies. LC methods have been reported to quantify isolated flavonoids or these compounds in complex biological matrices, such as herbal raw materials and extractive preparations. This work was designed, therefore, to develop an LC system to separate quercetin, luteolin and 3-
O-methylquercetin and to quantify them in extractive solutions from
Achyrocline satureioides. The main validation parameters of the method are also determined. The method showed linearity for quercetin and luteolin in the range 1–10 μg/ml. The aqueous and ethanol 80% extractive solutions showed linear response in the range 2.5–20 μl/ml and ethanol 40% extractive solution in the range 2.5–10 μl/ml. Precision and accuracy were determined for ethanol 80% extractive solution, in the concentration of 10 μl/ml. The LC method showed an excellent performance in separating the flavonoids quercetin, luteolin and 3-
O-methylquercetin in
A. satureioides extracts, since the presence of interference has been previously evaluated.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12008157</pmid><doi>10.1016/S0731-7085(01)00693-8</doi><tpages>7</tpages></addata></record> |
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| subjects | 3- O-Methylquercetin Achyrocline satureioides Analysis Biological and medical sciences Calibration Chromatography, Liquid Ethanol Flavonoids Flavonoids - analysis Flavonoids - isolation & purification General pharmacology Indicators and Reagents Luteolin Medical sciences Pharmacology. Drug treatments Plants, Medicinal - chemistry Quercetin Quercetin - analogs & derivatives Quercetin - analysis Quercetin - isolation & purification Reproducibility of Results Solutions Solvents Spectrophotometry, Ultraviolet Water |
| title | LC determination of flavonoids: separation of quercetin, luteolin and 3- O-methylquercetin in Achyrocline satureioides preparations |
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