Improved and validated method for the determination of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC and 11-nor-9-carboxy-THC in serum, and in human liver microsomal preparations using gas chromatography-mass spectrometry
A validated method for the quantification of Delta(9)-tetrahydrocannabinol (THC) and its main metabolites 11-hydroxy-tetrahydrocannabinol (OH-THC) and 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) in serum is presented. The substances were isolated by solid-phase extraction, derivatised by methyl...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2002-06, Vol.772 (2), p.239-248 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Steinmeyer, Stefan Bregel, Dietmar Warth, Stefan Kraemer, Thomas Moeller, Manfred R |
| description | A validated method for the quantification of Delta(9)-tetrahydrocannabinol (THC) and its main metabolites 11-hydroxy-tetrahydrocannabinol (OH-THC) and 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) in serum is presented. The substances were isolated by solid-phase extraction, derivatised by methylation, and analysed by means of GC-MS in the selected ion monitoring mode. Quantitation was achieved by the addition of deuterated analogues as internal standards. The method was linear up to 10 ng/ml for THC and OH-THC, and up to 50 ng/ml for THC-COOH. The limits of quantification were 0.62 ng/ml for THC, 0.68 ng/ml for OH-THC and 3.35 ng/ml for THC-COOH. The limits of detection for the least intensive ions were 0.52 ng/ml for THC, 0.49 ng/ml for OH-THC and 0.65 ng/ml for THC-COOH. The method was validated according to the requirements of the Journal of Chromatography B. The method has been routinely used on samples from drivers suspected of "driving under the influence". In addition to the forensic application, a cross-validation was carried out by applying the method developed for serum to human liver microsomal preparation samples. |
| doi_str_mv | 10.1016/S1570-0232(02)00102-2 |
| format | Article |
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The substances were isolated by solid-phase extraction, derivatised by methylation, and analysed by means of GC-MS in the selected ion monitoring mode. Quantitation was achieved by the addition of deuterated analogues as internal standards. The method was linear up to 10 ng/ml for THC and OH-THC, and up to 50 ng/ml for THC-COOH. The limits of quantification were 0.62 ng/ml for THC, 0.68 ng/ml for OH-THC and 3.35 ng/ml for THC-COOH. The limits of detection for the least intensive ions were 0.52 ng/ml for THC, 0.49 ng/ml for OH-THC and 0.65 ng/ml for THC-COOH. The method was validated according to the requirements of the Journal of Chromatography B. The method has been routinely used on samples from drivers suspected of "driving under the influence". In addition to the forensic application, a cross-validation was carried out by applying the method developed for serum to human liver microsomal preparation samples.</description><identifier>ISSN: 1570-0232</identifier><identifier>DOI: 10.1016/S1570-0232(02)00102-2</identifier><identifier>PMID: 12007768</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Dronabinol - analogs & derivatives ; Dronabinol - blood ; Dronabinol - metabolism ; Forensic Medicine ; Gas Chromatography-Mass Spectrometry - methods ; Humans ; Microsomes, Liver - metabolism ; Reference Standards ; Sensitivity and Specificity</subject><ispartof>Journal of chromatography. 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B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A validated method for the quantification of Delta(9)-tetrahydrocannabinol (THC) and its main metabolites 11-hydroxy-tetrahydrocannabinol (OH-THC) and 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) in serum is presented. The substances were isolated by solid-phase extraction, derivatised by methylation, and analysed by means of GC-MS in the selected ion monitoring mode. Quantitation was achieved by the addition of deuterated analogues as internal standards. The method was linear up to 10 ng/ml for THC and OH-THC, and up to 50 ng/ml for THC-COOH. The limits of quantification were 0.62 ng/ml for THC, 0.68 ng/ml for OH-THC and 3.35 ng/ml for THC-COOH. The limits of detection for the least intensive ions were 0.52 ng/ml for THC, 0.49 ng/ml for OH-THC and 0.65 ng/ml for THC-COOH. The method was validated according to the requirements of the Journal of Chromatography B. The method has been routinely used on samples from drivers suspected of "driving under the influence". In addition to the forensic application, a cross-validation was carried out by applying the method developed for serum to human liver microsomal preparation samples.</description><subject>Dronabinol - analogs & derivatives</subject><subject>Dronabinol - blood</subject><subject>Dronabinol - metabolism</subject><subject>Forensic Medicine</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Humans</subject><subject>Microsomes, Liver - metabolism</subject><subject>Reference Standards</subject><subject>Sensitivity and Specificity</subject><issn>1570-0232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UUtv1DAQzgFES-EngHxCu1Jd_EjW2SNa6EOq1APlvJrYk41RbAfbWZHf2z-CtbQ9zXwPzXyaqapPnF1xxjdff_JGMcqEFCsm1oxxJqh4U52_0mfV-5R-F0ExJd9VZ1wwptSmPa-e7twUwxENAW_IEUZrIBfkMA_BkD5EkgckBjNGZz1kGzwJPfmOY4bVdk0z5gjDYmLQ4D101oeRrB5vd-tLwjk9KX8XWojThkL5EOmWaojdi2A9SRhnd3myFDTMDjwZ7REjcVbHkIKDkUwRJ4inDInMyfoDOUAieohFzuEQYRoW6iAlkibUudAl3fKhetvDmPDjc72ofl3_eNzd0vuHm7vdt3s6CaYylbLutIHWdD12vWZbkKZu5FbquhW8bQ3rjOgMN0oA8rqDVpqmbkFA6RqF8qL68n9uueifGVPeO5s0jiN4DHPaK75p64bJYvz8bJw7h2Y_ResgLvuXt8h_4kuS6g</recordid><startdate>20020605</startdate><enddate>20020605</enddate><creator>Steinmeyer, Stefan</creator><creator>Bregel, Dietmar</creator><creator>Warth, Stefan</creator><creator>Kraemer, Thomas</creator><creator>Moeller, Manfred R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020605</creationdate><title>Improved and validated method for the determination of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC and 11-nor-9-carboxy-THC in serum, and in human liver microsomal preparations using gas chromatography-mass spectrometry</title><author>Steinmeyer, Stefan ; Bregel, Dietmar ; Warth, Stefan ; Kraemer, Thomas ; Moeller, Manfred R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-334bcda8dbfebfc09a3d45393c482188d0bd2bd1d72ae14ba83d548a2aa8357e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Dronabinol - analogs & derivatives</topic><topic>Dronabinol - blood</topic><topic>Dronabinol - metabolism</topic><topic>Forensic Medicine</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>Humans</topic><topic>Microsomes, Liver - metabolism</topic><topic>Reference Standards</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Steinmeyer, Stefan</creatorcontrib><creatorcontrib>Bregel, Dietmar</creatorcontrib><creatorcontrib>Warth, Stefan</creatorcontrib><creatorcontrib>Kraemer, Thomas</creatorcontrib><creatorcontrib>Moeller, Manfred R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Steinmeyer, Stefan</au><au>Bregel, Dietmar</au><au>Warth, Stefan</au><au>Kraemer, Thomas</au><au>Moeller, Manfred R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved and validated method for the determination of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC and 11-nor-9-carboxy-THC in serum, and in human liver microsomal preparations using gas chromatography-mass spectrometry</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2002-06-05</date><risdate>2002</risdate><volume>772</volume><issue>2</issue><spage>239</spage><epage>248</epage><pages>239-248</pages><issn>1570-0232</issn><abstract>A validated method for the quantification of Delta(9)-tetrahydrocannabinol (THC) and its main metabolites 11-hydroxy-tetrahydrocannabinol (OH-THC) and 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) in serum is presented. The substances were isolated by solid-phase extraction, derivatised by methylation, and analysed by means of GC-MS in the selected ion monitoring mode. Quantitation was achieved by the addition of deuterated analogues as internal standards. The method was linear up to 10 ng/ml for THC and OH-THC, and up to 50 ng/ml for THC-COOH. The limits of quantification were 0.62 ng/ml for THC, 0.68 ng/ml for OH-THC and 3.35 ng/ml for THC-COOH. The limits of detection for the least intensive ions were 0.52 ng/ml for THC, 0.49 ng/ml for OH-THC and 0.65 ng/ml for THC-COOH. The method was validated according to the requirements of the Journal of Chromatography B. The method has been routinely used on samples from drivers suspected of "driving under the influence". In addition to the forensic application, a cross-validation was carried out by applying the method developed for serum to human liver microsomal preparation samples.</abstract><cop>Netherlands</cop><pmid>12007768</pmid><doi>10.1016/S1570-0232(02)00102-2</doi><tpages>10</tpages></addata></record> |
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| issn | 1570-0232 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Dronabinol - analogs & derivatives Dronabinol - blood Dronabinol - metabolism Forensic Medicine Gas Chromatography-Mass Spectrometry - methods Humans Microsomes, Liver - metabolism Reference Standards Sensitivity and Specificity |
| title | Improved and validated method for the determination of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC and 11-nor-9-carboxy-THC in serum, and in human liver microsomal preparations using gas chromatography-mass spectrometry |
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