Localization of calbindin D-28K in the otoconia of lizard Podarcis sicula
The membranous labyrinth of lizard Podarcis sicula contains calcite and aragonite crystals. Saccule, utricle and lagena contain calcite crystals while aragonite crystals are present only in the saccule where they are very abundant. We have recently demonstrated the presence of calbindin D-28K in the...
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| Veröffentlicht in: | Hearing research 2004-03, Vol.189 (1), p.76-82 |
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| creator | Piscopo, Marina Avallone, Bice D’Angelo, Loredana Fascio, Umberto Balsamo, Giuseppe Marmo, Francesco |
| description | The membranous labyrinth of lizard
Podarcis sicula contains calcite and aragonite crystals. Saccule, utricle and lagena contain calcite crystals while aragonite crystals are present only in the saccule where they are very abundant. We have recently demonstrated the presence of calbindin D-28K in the organic matrix of lizard
P. sicula otoconia. In order to define its localization, since calbindin modulates cellular Ca
2+ level, otoconia from utricle and lagena were collected separately from those from saccule and then otoconial proteins were extracted. Immunoblot assay on proteins extracted from the otoconia and confocal laser scanning microscope analyses of otoconia using monoclonal anti-calbindin D-28K antibodies indicated that calbindin D-28K is a protein typical of aragonite crystals. |
| doi_str_mv | 10.1016/S0378-5955(03)00366-6 |
| format | Article |
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Podarcis sicula contains calcite and aragonite crystals. Saccule, utricle and lagena contain calcite crystals while aragonite crystals are present only in the saccule where they are very abundant. We have recently demonstrated the presence of calbindin D-28K in the organic matrix of lizard
P. sicula otoconia. In order to define its localization, since calbindin modulates cellular Ca
2+ level, otoconia from utricle and lagena were collected separately from those from saccule and then otoconial proteins were extracted. Immunoblot assay on proteins extracted from the otoconia and confocal laser scanning microscope analyses of otoconia using monoclonal anti-calbindin D-28K antibodies indicated that calbindin D-28K is a protein typical of aragonite crystals.</description><identifier>ISSN: 0378-5955</identifier><identifier>EISSN: 1878-5891</identifier><identifier>DOI: 10.1016/S0378-5955(03)00366-6</identifier><identifier>PMID: 14987754</identifier><identifier>CODEN: HERED3</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal ; Aragonite ; Biological and medical sciences ; Blotting, Western ; Calbindin D-28K ; Calbindins ; Fundamental and applied biological sciences. Psychology ; Inner ear ; Lizards - metabolism ; Microscopy, Confocal ; Microscopy, Electron, Scanning ; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration ; Otoconia ; Otolithic Membrane - metabolism ; Otolithic Membrane - ultrastructure ; Podarcis sicula ; S100 Calcium Binding Protein G - metabolism ; Tissue Distribution ; Vertebrates: nervous system and sense organs</subject><ispartof>Hearing research, 2004-03, Vol.189 (1), p.76-82</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-cc86a397ac6133035b5af0cf38c703fac109a3b4224c3c21c1ada3b9867b0f903</citedby><cites>FETCH-LOGICAL-c391t-cc86a397ac6133035b5af0cf38c703fac109a3b4224c3c21c1ada3b9867b0f903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-5955(03)00366-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15499292$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14987754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Piscopo, Marina</creatorcontrib><creatorcontrib>Avallone, Bice</creatorcontrib><creatorcontrib>D’Angelo, Loredana</creatorcontrib><creatorcontrib>Fascio, Umberto</creatorcontrib><creatorcontrib>Balsamo, Giuseppe</creatorcontrib><creatorcontrib>Marmo, Francesco</creatorcontrib><title>Localization of calbindin D-28K in the otoconia of lizard Podarcis sicula</title><title>Hearing research</title><addtitle>Hear Res</addtitle><description>The membranous labyrinth of lizard
Podarcis sicula contains calcite and aragonite crystals. Saccule, utricle and lagena contain calcite crystals while aragonite crystals are present only in the saccule where they are very abundant. We have recently demonstrated the presence of calbindin D-28K in the organic matrix of lizard
P. sicula otoconia. In order to define its localization, since calbindin modulates cellular Ca
2+ level, otoconia from utricle and lagena were collected separately from those from saccule and then otoconial proteins were extracted. Immunoblot assay on proteins extracted from the otoconia and confocal laser scanning microscope analyses of otoconia using monoclonal anti-calbindin D-28K antibodies indicated that calbindin D-28K is a protein typical of aragonite crystals.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Aragonite</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Calbindin D-28K</subject><subject>Calbindins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inner ear</subject><subject>Lizards - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron, Scanning</subject><subject>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</subject><subject>Otoconia</subject><subject>Otolithic Membrane - metabolism</subject><subject>Otolithic Membrane - ultrastructure</subject><subject>Podarcis sicula</subject><subject>S100 Calcium Binding Protein G - metabolism</subject><subject>Tissue Distribution</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0378-5955</issn><issn>1878-5891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUQIMoOj4-QelG0UX1pmnSZCXiGwcU1HVIb1OMdBpNOoJ-vakz6NLVfXDug0PILoVjClScPAKrZM4V54fAjgCYELlYIRMqx7ZUdJVMfpENshnjKwDlrCzWyQYtlawqXk7I7dSj6dyXGZzvM99mqapd37g-u8gLeZelZHixmR88-t6ZERnx0GQPvjEBXcyiw3lntslaa7pod5ZxizxfXT6d3-TT--vb87NpjkzRIUeUwjBVGRSUMWC85qYFbJnEClhrkIIyrC6LokSGBUVqmlQrKaoaWgVsixws9r4F_z63cdAzF9F2nemtn0ddUSFZCUUC-QLE4GMMttVvwc1M-NQU9OhQ_zjUoyANTP841CLN7S0PzOuZbf6mltISsL8ETEy62mD6pOGP46VShRofOF1wNun4cDboiM72aBsXLA668e6fV74BWaSMuA</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Piscopo, Marina</creator><creator>Avallone, Bice</creator><creator>D’Angelo, Loredana</creator><creator>Fascio, Umberto</creator><creator>Balsamo, Giuseppe</creator><creator>Marmo, Francesco</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>20040301</creationdate><title>Localization of calbindin D-28K in the otoconia of lizard Podarcis sicula</title><author>Piscopo, Marina ; Avallone, Bice ; D’Angelo, Loredana ; Fascio, Umberto ; Balsamo, Giuseppe ; Marmo, Francesco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-cc86a397ac6133035b5af0cf38c703fac109a3b4224c3c21c1ada3b9867b0f903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Aragonite</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Calbindin D-28K</topic><topic>Calbindins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inner ear</topic><topic>Lizards - metabolism</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Electron, Scanning</topic><topic>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</topic><topic>Otoconia</topic><topic>Otolithic Membrane - metabolism</topic><topic>Otolithic Membrane - ultrastructure</topic><topic>Podarcis sicula</topic><topic>S100 Calcium Binding Protein G - metabolism</topic><topic>Tissue Distribution</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Piscopo, Marina</creatorcontrib><creatorcontrib>Avallone, Bice</creatorcontrib><creatorcontrib>D’Angelo, Loredana</creatorcontrib><creatorcontrib>Fascio, Umberto</creatorcontrib><creatorcontrib>Balsamo, Giuseppe</creatorcontrib><creatorcontrib>Marmo, Francesco</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Hearing research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Piscopo, Marina</au><au>Avallone, Bice</au><au>D’Angelo, Loredana</au><au>Fascio, Umberto</au><au>Balsamo, Giuseppe</au><au>Marmo, Francesco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of calbindin D-28K in the otoconia of lizard Podarcis sicula</atitle><jtitle>Hearing research</jtitle><addtitle>Hear Res</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>189</volume><issue>1</issue><spage>76</spage><epage>82</epage><pages>76-82</pages><issn>0378-5955</issn><eissn>1878-5891</eissn><coden>HERED3</coden><abstract>The membranous labyrinth of lizard
Podarcis sicula contains calcite and aragonite crystals. Saccule, utricle and lagena contain calcite crystals while aragonite crystals are present only in the saccule where they are very abundant. We have recently demonstrated the presence of calbindin D-28K in the organic matrix of lizard
P. sicula otoconia. In order to define its localization, since calbindin modulates cellular Ca
2+ level, otoconia from utricle and lagena were collected separately from those from saccule and then otoconial proteins were extracted. Immunoblot assay on proteins extracted from the otoconia and confocal laser scanning microscope analyses of otoconia using monoclonal anti-calbindin D-28K antibodies indicated that calbindin D-28K is a protein typical of aragonite crystals.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>14987754</pmid><doi>10.1016/S0378-5955(03)00366-6</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Antibodies, Monoclonal Aragonite Biological and medical sciences Blotting, Western Calbindin D-28K Calbindins Fundamental and applied biological sciences. Psychology Inner ear Lizards - metabolism Microscopy, Confocal Microscopy, Electron, Scanning Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration Otoconia Otolithic Membrane - metabolism Otolithic Membrane - ultrastructure Podarcis sicula S100 Calcium Binding Protein G - metabolism Tissue Distribution Vertebrates: nervous system and sense organs |
| title | Localization of calbindin D-28K in the otoconia of lizard Podarcis sicula |
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