In vitro analysis of neuron-glial cell interactions during cellular migration
We used time-lapse microscopy to study the in vitro migration of neuronal cells from developing chick ciliary ganglion. These cells, when dissociated and cultured in a chemically defined medium, are able to migrate and to associate into clusters. We focused our attention on the study of the distribu...
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| Veröffentlicht in: | European biophysics journal 2002-05, Vol.31 (2), p.81-88 |
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| creator | Distasi, Carla Ariano, Paolo Zamburlin, Pollyanna Ferraro, Mario |
| description | We used time-lapse microscopy to study the in vitro migration of neuronal cells from developing chick ciliary ganglion. These cells, when dissociated and cultured in a chemically defined medium, are able to migrate and to associate into clusters. We focused our attention on the study of the distribution of neuronal velocity components. Quantitative analysis of cell trajectories allowed us to demonstrate that, in many cells, velocities are well described by the Langevin equation, when deterministic components of the forces acting on the cells are taken into account. We also have shown that the majority of neurons whose movement is not purely random migrate in association with glial cells. We conclude that glial cells, by guiding neurons during migration, play an important role in the cell organization in vitro. |
| doi_str_mv | 10.1007/s00249-001-0194-y |
| format | Article |
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These cells, when dissociated and cultured in a chemically defined medium, are able to migrate and to associate into clusters. We focused our attention on the study of the distribution of neuronal velocity components. Quantitative analysis of cell trajectories allowed us to demonstrate that, in many cells, velocities are well described by the Langevin equation, when deterministic components of the forces acting on the cells are taken into account. We also have shown that the majority of neurons whose movement is not purely random migrate in association with glial cells. We conclude that glial cells, by guiding neurons during migration, play an important role in the cell organization in vitro.</description><identifier>ISSN: 0175-7571</identifier><identifier>EISSN: 1432-1017</identifier><identifier>DOI: 10.1007/s00249-001-0194-y</identifier><identifier>PMID: 12012111</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Attention ; Axon guidance ; Biophysical Phenomena ; Biophysics ; Cell Communication ; Cell interactions ; Cell migration ; Cell Movement ; Cells, Cultured ; Cellular biology ; Chick Embryo ; ciliary ganglion ; Glial cells ; Humans ; Mathematical models ; Microscopy ; Microscopy, Video ; Models, Theoretical ; Movement ; Neuroglia - cytology ; Neuronal-glial interactions ; Neurons ; Neurons - cytology ; Normal Distribution ; Software</subject><ispartof>European biophysics journal, 2002-05, Vol.31 (2), p.81-88</ispartof><rights>EBSA 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-d1fd1b7729d95b51c588c264bb12370a1ce6c35aaf3d7d2fd030295b626dc43d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12012111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Distasi, Carla</creatorcontrib><creatorcontrib>Ariano, Paolo</creatorcontrib><creatorcontrib>Zamburlin, Pollyanna</creatorcontrib><creatorcontrib>Ferraro, Mario</creatorcontrib><title>In vitro analysis of neuron-glial cell interactions during cellular migration</title><title>European biophysics journal</title><addtitle>Eur Biophys J</addtitle><description>We used time-lapse microscopy to study the in vitro migration of neuronal cells from developing chick ciliary ganglion. These cells, when dissociated and cultured in a chemically defined medium, are able to migrate and to associate into clusters. We focused our attention on the study of the distribution of neuronal velocity components. Quantitative analysis of cell trajectories allowed us to demonstrate that, in many cells, velocities are well described by the Langevin equation, when deterministic components of the forces acting on the cells are taken into account. We also have shown that the majority of neurons whose movement is not purely random migrate in association with glial cells. 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| subjects | Animals Attention Axon guidance Biophysical Phenomena Biophysics Cell Communication Cell interactions Cell migration Cell Movement Cells, Cultured Cellular biology Chick Embryo ciliary ganglion Glial cells Humans Mathematical models Microscopy Microscopy, Video Models, Theoretical Movement Neuroglia - cytology Neuronal-glial interactions Neurons Neurons - cytology Normal Distribution Software |
| title | In vitro analysis of neuron-glial cell interactions during cellular migration |
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