Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology
Objectives: The use of antibiotics for the treatment of infectious diseases has led to important changes in the structure of pathogenic bacterial populations. However, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2004-03, Vol.53 (3), p.432-434 |
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| creator | Alonso, Ana Morales, Gracia Escalante, Ricardo Campanario, Ester Sastre, Leandro Martinez, Jose L. |
| description | Objectives: The use of antibiotics for the treatment of infectious diseases has led to important changes in the structure of pathogenic bacterial populations. However, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic-resistant strains in antibiotic-free environments. To test the effect of antibiotic resistance on bacterial fitness, we analysed the effect of the overproduction of the multidrug efflux pump SmeDEF on the physiology of Stenotrophomonas maltophilia. SmeDEF confers resistance to antibiotics belonging to different structural families, and its overexpression is associated with an antibiotic resistance phenotype in clinical isolates of S. maltophilia. Results: Two S. maltophilia isogenic strains were analysed: the wild-type strain D457 and strain D457R, which is a SmeDEF overproducer. In co-culture experiments, under non-selective pressure the wild-type strain displaced the mutant strain D457R. Metabolic profiling showed that SmeDEF overproduction leads to several changes in S. maltophilia metabolism. Using a Dictyostelium discoideum model of bacterial virulence, we found overexpression of SmeDEF to be associated with a reduction in S. maltophilia virulence. Conclusions: Together, these data indicate that overexpression of the multidrug efflux pump SmeDEF causes a metabolic burden for S. maltophilia. |
| doi_str_mv | 10.1093/jac/dkh074 |
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However, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic-resistant strains in antibiotic-free environments. To test the effect of antibiotic resistance on bacterial fitness, we analysed the effect of the overproduction of the multidrug efflux pump SmeDEF on the physiology of Stenotrophomonas maltophilia. SmeDEF confers resistance to antibiotics belonging to different structural families, and its overexpression is associated with an antibiotic resistance phenotype in clinical isolates of S. maltophilia. Results: Two S. maltophilia isogenic strains were analysed: the wild-type strain D457 and strain D457R, which is a SmeDEF overproducer. In co-culture experiments, under non-selective pressure the wild-type strain displaced the mutant strain D457R. Metabolic profiling showed that SmeDEF overproduction leads to several changes in S. maltophilia metabolism. Using a Dictyostelium discoideum model of bacterial virulence, we found overexpression of SmeDEF to be associated with a reduction in S. maltophilia virulence. Conclusions: Together, these data indicate that overexpression of the multidrug efflux pump SmeDEF causes a metabolic burden for S. maltophilia.</description><identifier>ISSN: 0305-7453</identifier><identifier>ISSN: 1460-2091</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkh074</identifier><identifier>PMID: 14739147</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; antibiotic resistance ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; bacterial fitness ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Biological and medical sciences ; Culture Media ; Dictyostelium - genetics ; Dictyostelium - growth & development ; Dictyostelium discoideum ; Drug Resistance, Multiple, Bacterial - genetics ; MDR ; Medical sciences ; Membrane Transport Proteins - biosynthesis ; Membrane Transport Proteins - genetics ; metabolic profiling ; Pharmacology. Drug treatments ; Phenotype ; S. maltophilia ; Stenotrophomonas maltophilia ; Stenotrophomonas maltophilia - genetics ; Stenotrophomonas maltophilia - growth & development ; Stenotrophomonas maltophilia - physiology ; virulence ; Virulence - physiology</subject><ispartof>Journal of antimicrobial chemotherapy, 2004-03, Vol.53 (3), p.432-434</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Mar 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-66e3de82e4dfaf2d38acbbc45584eb78ec45984dcf7fc6493244dea387aa263f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15667206$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14739147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alonso, Ana</creatorcontrib><creatorcontrib>Morales, Gracia</creatorcontrib><creatorcontrib>Escalante, Ricardo</creatorcontrib><creatorcontrib>Campanario, Ester</creatorcontrib><creatorcontrib>Sastre, Leandro</creatorcontrib><creatorcontrib>Martinez, Jose L.</creatorcontrib><title>Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J. Antimicrob. Chemother</addtitle><description>Objectives: The use of antibiotics for the treatment of infectious diseases has led to important changes in the structure of pathogenic bacterial populations. However, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic-resistant strains in antibiotic-free environments. To test the effect of antibiotic resistance on bacterial fitness, we analysed the effect of the overproduction of the multidrug efflux pump SmeDEF on the physiology of Stenotrophomonas maltophilia. SmeDEF confers resistance to antibiotics belonging to different structural families, and its overexpression is associated with an antibiotic resistance phenotype in clinical isolates of S. maltophilia. Results: Two S. maltophilia isogenic strains were analysed: the wild-type strain D457 and strain D457R, which is a SmeDEF overproducer. In co-culture experiments, under non-selective pressure the wild-type strain displaced the mutant strain D457R. Metabolic profiling showed that SmeDEF overproduction leads to several changes in S. maltophilia metabolism. Using a Dictyostelium discoideum model of bacterial virulence, we found overexpression of SmeDEF to be associated with a reduction in S. maltophilia virulence. Conclusions: Together, these data indicate that overexpression of the multidrug efflux pump SmeDEF causes a metabolic burden for S. maltophilia.</description><subject>Animals</subject><subject>antibiotic resistance</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>bacterial fitness</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Culture Media</subject><subject>Dictyostelium - genetics</subject><subject>Dictyostelium - growth & development</subject><subject>Dictyostelium discoideum</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>MDR</subject><subject>Medical sciences</subject><subject>Membrane Transport Proteins - biosynthesis</subject><subject>Membrane Transport Proteins - genetics</subject><subject>metabolic profiling</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenotype</subject><subject>S. maltophilia</subject><subject>Stenotrophomonas maltophilia</subject><subject>Stenotrophomonas maltophilia - genetics</subject><subject>Stenotrophomonas maltophilia - growth & development</subject><subject>Stenotrophomonas maltophilia - physiology</subject><subject>virulence</subject><subject>Virulence - physiology</subject><issn>0305-7453</issn><issn>1460-2091</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9rFDEYBvAgil1bL34ACUI9CGOTSSaZOUr_bIVKkVaUvYRM5k0328lkTGZk99sb2cWCFy9JXvLjgZcHoTeUfKSkYWcbbc66xzWR_BlaUC5IUZKGPkcLwkhVSF6xI_QqpQ0hRFSifomOKJesyccCtbe_IMJ2jJCSCwMOFk9rwH7uJ9fF-QGDtf28xePsR3zn4eLyCjs_ahcTvptgCFMM4zr4MOiEve6nPLneaTyudzmwDw-7E_TC6j7B68N9jL5dXd6fXxc3t8vP559uCsN5NRVCAOugLoF3VtuyY7U2bWt4VdUcWllDfjY174yV1gjesJLzDjSrpdalYJYdo_f73DGGnzOkSXmXDPS9HiDMSUkqJCsp-y-kUlJZC5Lhu3_gJsxxyEuokkohalLJjD7skYkhpQhWjdF5HXeKEvWnH5X7Uft-Mn57SJxbD90TPRSSwekB6GR0b6MejEtPrhJClkRkV-ydSxNs__7r-KjylrJS1z9WaiW_rJbfl0v1lf0G312qoA</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Alonso, Ana</creator><creator>Morales, Gracia</creator><creator>Escalante, Ricardo</creator><creator>Campanario, Ester</creator><creator>Sastre, Leandro</creator><creator>Martinez, Jose L.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040301</creationdate><title>Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology</title><author>Alonso, Ana ; Morales, Gracia ; Escalante, Ricardo ; Campanario, Ester ; Sastre, Leandro ; Martinez, Jose L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-66e3de82e4dfaf2d38acbbc45584eb78ec45984dcf7fc6493244dea387aa263f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>antibiotic resistance</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>bacterial fitness</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Culture Media</topic><topic>Dictyostelium - genetics</topic><topic>Dictyostelium - growth & development</topic><topic>Dictyostelium discoideum</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>MDR</topic><topic>Medical sciences</topic><topic>Membrane Transport Proteins - biosynthesis</topic><topic>Membrane Transport Proteins - genetics</topic><topic>metabolic profiling</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenotype</topic><topic>S. maltophilia</topic><topic>Stenotrophomonas maltophilia</topic><topic>Stenotrophomonas maltophilia - genetics</topic><topic>Stenotrophomonas maltophilia - growth & development</topic><topic>Stenotrophomonas maltophilia - physiology</topic><topic>virulence</topic><topic>Virulence - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alonso, Ana</creatorcontrib><creatorcontrib>Morales, Gracia</creatorcontrib><creatorcontrib>Escalante, Ricardo</creatorcontrib><creatorcontrib>Campanario, Ester</creatorcontrib><creatorcontrib>Sastre, Leandro</creatorcontrib><creatorcontrib>Martinez, Jose L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alonso, Ana</au><au>Morales, Gracia</au><au>Escalante, Ricardo</au><au>Campanario, Ester</au><au>Sastre, Leandro</au><au>Martinez, Jose L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J. Antimicrob. Chemother</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>53</volume><issue>3</issue><spage>432</spage><epage>434</epage><pages>432-434</pages><issn>0305-7453</issn><issn>1460-2091</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives: The use of antibiotics for the treatment of infectious diseases has led to important changes in the structure of pathogenic bacterial populations. However, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic-resistant strains in antibiotic-free environments. To test the effect of antibiotic resistance on bacterial fitness, we analysed the effect of the overproduction of the multidrug efflux pump SmeDEF on the physiology of Stenotrophomonas maltophilia. SmeDEF confers resistance to antibiotics belonging to different structural families, and its overexpression is associated with an antibiotic resistance phenotype in clinical isolates of S. maltophilia. Results: Two S. maltophilia isogenic strains were analysed: the wild-type strain D457 and strain D457R, which is a SmeDEF overproducer. In co-culture experiments, under non-selective pressure the wild-type strain displaced the mutant strain D457R. Metabolic profiling showed that SmeDEF overproduction leads to several changes in S. maltophilia metabolism. Using a Dictyostelium discoideum model of bacterial virulence, we found overexpression of SmeDEF to be associated with a reduction in S. maltophilia virulence. Conclusions: Together, these data indicate that overexpression of the multidrug efflux pump SmeDEF causes a metabolic burden for S. maltophilia.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>14739147</pmid><doi>10.1093/jac/dkh074</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals antibiotic resistance Antibiotics. Antiinfectious agents. Antiparasitic agents bacterial fitness Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Biological and medical sciences Culture Media Dictyostelium - genetics Dictyostelium - growth & development Dictyostelium discoideum Drug Resistance, Multiple, Bacterial - genetics MDR Medical sciences Membrane Transport Proteins - biosynthesis Membrane Transport Proteins - genetics metabolic profiling Pharmacology. Drug treatments Phenotype S. maltophilia Stenotrophomonas maltophilia Stenotrophomonas maltophilia - genetics Stenotrophomonas maltophilia - growth & development Stenotrophomonas maltophilia - physiology virulence Virulence - physiology |
| title | Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology |
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