Detection of the M30 neoepitope as a new tool to quantify liver apoptosis: Timing and patterns of positivity on frozen and paraffin-embedded sections
One of the first stages of apoptosis is cytokeratin cleavage mediated by caspases, which is associated with the expression of a neoepitope, the cleavage site of cytokeratin 18, identifiable by the M30 monoclonal antibody. The aim of this study was to evaluate the timing of neoantigen expression and...
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| Veröffentlicht in: | American journal of clinical pathology 2004-02, Vol.121 (2), p.211-219 |
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| creator | GRASSI, Alberto SUSCA, Micaela FERRI, Silvia GABUSI, Elena D'ERRICO, Antonia FARINA, Gabriele MACCARIELLO, Silvana ZAULI, Daniela BIANCHI, Francesco B BALLARDINI, Giorgio |
| description | One of the first stages of apoptosis is cytokeratin cleavage mediated by caspases, which is associated with the expression of a neoepitope, the cleavage site of cytokeratin 18, identifiable by the M30 monoclonal antibody. The aim of this study was to evaluate the timing of neoantigen expression and its modifications in the various morphologic stages of apoptosis on frozen and paraffin-embedded sections from liver biopsies of patients with chronic hepatitis or transplanted liver. The appearance of this neoepitope coincides with the gradual disappearance of cytokeratins, with the appearance of nuclear DNA fragmentation, and with the presence of Councilman bodies. The staining patterns on paraffin-embedded sections of liver specimens were similar to those found in frozen sections, with a reduced sensitivity. The M30 antibody is correlated with apoptosis, and its specificity for epithelial cells makes this method the first choice for routine evaluation of apoptosis in liver epithelial cells. |
| doi_str_mv | 10.1309/UK621LFJ4FX07KDE |
| format | Article |
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The aim of this study was to evaluate the timing of neoantigen expression and its modifications in the various morphologic stages of apoptosis on frozen and paraffin-embedded sections from liver biopsies of patients with chronic hepatitis or transplanted liver. The appearance of this neoepitope coincides with the gradual disappearance of cytokeratins, with the appearance of nuclear DNA fragmentation, and with the presence of Councilman bodies. The staining patterns on paraffin-embedded sections of liver specimens were similar to those found in frozen sections, with a reduced sensitivity. The M30 antibody is correlated with apoptosis, and its specificity for epithelial cells makes this method the first choice for routine evaluation of apoptosis in liver epithelial cells.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1309/UK621LFJ4FX07KDE</identifier><identifier>PMID: 14983934</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Antibodies, Monoclonal ; Biological and medical sciences ; Biomarkers - analysis ; Cell Nucleus - metabolism ; Cell Nucleus - pathology ; Cytoplasm - metabolism ; Cytoplasm - pathology ; DNA Fragmentation ; Epitope Mapping ; Fluorescent Antibody Technique, Indirect ; Frozen Sections ; Hepatitis, Chronic - metabolism ; Hepatitis, Chronic - pathology ; Hepatocytes - metabolism ; Hepatocytes - pathology ; Humans ; In Situ Nick-End Labeling ; Investigative techniques, diagnostic techniques (general aspects) ; Keratins - immunology ; Keratins - metabolism ; Liver - metabolism ; Liver - pathology ; Liver Transplantation ; Medical sciences ; Paraffin Embedding ; Pathology. 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Miscellaneous investigative techniques</subject><ispartof>American journal of clinical pathology, 2004-02, Vol.121 (2), p.211-219</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27913,27914</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15561109$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14983934$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GRASSI, Alberto</creatorcontrib><creatorcontrib>SUSCA, Micaela</creatorcontrib><creatorcontrib>FERRI, Silvia</creatorcontrib><creatorcontrib>GABUSI, Elena</creatorcontrib><creatorcontrib>D'ERRICO, Antonia</creatorcontrib><creatorcontrib>FARINA, Gabriele</creatorcontrib><creatorcontrib>MACCARIELLO, Silvana</creatorcontrib><creatorcontrib>ZAULI, Daniela</creatorcontrib><creatorcontrib>BIANCHI, Francesco B</creatorcontrib><creatorcontrib>BALLARDINI, Giorgio</creatorcontrib><title>Detection of the M30 neoepitope as a new tool to quantify liver apoptosis: Timing and patterns of positivity on frozen and paraffin-embedded sections</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>One of the first stages of apoptosis is cytokeratin cleavage mediated by caspases, which is associated with the expression of a neoepitope, the cleavage site of cytokeratin 18, identifiable by the M30 monoclonal antibody. The aim of this study was to evaluate the timing of neoantigen expression and its modifications in the various morphologic stages of apoptosis on frozen and paraffin-embedded sections from liver biopsies of patients with chronic hepatitis or transplanted liver. The appearance of this neoepitope coincides with the gradual disappearance of cytokeratins, with the appearance of nuclear DNA fragmentation, and with the presence of Councilman bodies. The staining patterns on paraffin-embedded sections of liver specimens were similar to those found in frozen sections, with a reduced sensitivity. The M30 antibody is correlated with apoptosis, and its specificity for epithelial cells makes this method the first choice for routine evaluation of apoptosis in liver epithelial cells.</description><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - pathology</subject><subject>Cytoplasm - metabolism</subject><subject>Cytoplasm - pathology</subject><subject>DNA Fragmentation</subject><subject>Epitope Mapping</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Frozen Sections</subject><subject>Hepatitis, Chronic - metabolism</subject><subject>Hepatitis, Chronic - pathology</subject><subject>Hepatocytes - metabolism</subject><subject>Hepatocytes - pathology</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Keratins - immunology</subject><subject>Keratins - metabolism</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver Transplantation</subject><subject>Medical sciences</subject><subject>Paraffin Embedding</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. 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Cytology. Biochemistry. Spectrometry. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Antibodies, Monoclonal Biological and medical sciences Biomarkers - analysis Cell Nucleus - metabolism Cell Nucleus - pathology Cytoplasm - metabolism Cytoplasm - pathology DNA Fragmentation Epitope Mapping Fluorescent Antibody Technique, Indirect Frozen Sections Hepatitis, Chronic - metabolism Hepatitis, Chronic - pathology Hepatocytes - metabolism Hepatocytes - pathology Humans In Situ Nick-End Labeling Investigative techniques, diagnostic techniques (general aspects) Keratins - immunology Keratins - metabolism Liver - metabolism Liver - pathology Liver Transplantation Medical sciences Paraffin Embedding Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques |
| title | Detection of the M30 neoepitope as a new tool to quantify liver apoptosis: Timing and patterns of positivity on frozen and paraffin-embedded sections |
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