Calpain inhibition by cerebrospinal fluid and effects of calpain on intrathecal nerve tissue
The effects of calpain on intrathecal nerve tissue in the rabbit were investigated. To evaluate the chemonucleolytic side effect of calpain on nerve tissue in the event of accidental intrathecal calpain injection. Calpain has a degradative effect on proteoglycans, and as previously shown, it is asso...
Gespeichert in:
| Veröffentlicht in: | Spine (Philadelphia, Pa. 1976) Pa. 1976), 2002-05, Vol.27 (10), p.1077-1081 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1081 |
|---|---|
| container_issue | 10 |
| container_start_page | 1077 |
| container_title | Spine (Philadelphia, Pa. 1976) |
| container_volume | 27 |
| creator | KODAMA, Hirotaka SHIMIZU, Katsuji BANNO, Yoshiko NONOMURA, Yuka OSHITA, Hidefumi AKAIKE, Atsushi |
| description | The effects of calpain on intrathecal nerve tissue in the rabbit were investigated.
To evaluate the chemonucleolytic side effect of calpain on nerve tissue in the event of accidental intrathecal calpain injection.
Calpain has a degradative effect on proteoglycans, and as previously shown, it is associated with chemonucleolytic action in the rabbit. However, its effect on nerve tissue in the event of accidental intrathecal injection is not clear.
The inhibitory activity of cerebrospinal fluid against calpain was measured in human cerebrospinal fluid using mu-calpain, and in different cerebrospinal fluid fractions separated by molecular filtration. The presence of the endogenous calpain inhibitor, calpastatin, in human cerebrospinal fluid was examined by Western blotting with anticalpastatin antibody. After intrathecal application of calpain in rabbits, the spinal cord nerve tissue was examined by light microscopy.
Cerebrospinal fluid inhibited the enzyme reaction of calpain at its normal concentration. Immunoblotting with anticalpastatin antibody did not yield positive staining. After the intrathecal application of calpain, there was no evidence of degeneration in the nerve tissue of the spinal cord.
This study suggests that in the event of accidental intrathecal injection of calpain for chemonucleolysis, the enzyme activity of calpain will be neutralized by cerebrospinal fluid, and the calpain should not cause unwanted side effects in chemonucleolysis. |
| doi_str_mv | 10.1097/00007632-200205150-00013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71672314</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71672314</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-1a1e029b445d616eca985f59dc8e11b686c607cf57a62964c2d3e6bb74dc4e9f3</originalsourceid><addsrcrecordid>eNpFkF1LwzAUQIMobk7_guRF36q5TZO0jzL8goEv-iaENL1hka6dSSv4781cdXkJXM65IYcQCuwGWKVuWTpK8jzLGcuZAMGyNAF-ROYg8jIDENUxmTMuE1JwOSNnMX4kRHKoTskMkleAEnPyvjTt1viO-m7taz_4vqP1N7UYsA593PrOtNS1o2-o6RqKzqEdIu0dtZPY79whmGGNaUQ7DF9IBx_jiOfkxJk24sV0L8jbw_3r8ilbvTw-L-9WmeWKDRkYQJZXdVGIRoJMa6pSOFE1tkSAWpbSSqasE8rIvJKFzRuOsq5V0dgCK8cX5Hq_dxv6zxHjoDc-Wmxb02E_Rq1AqpxDkcByD9r0txjQ6W3wGxO-NTC9K6v_yur_svq3bFIvpzfGeoPNQZxSJuBqAkxMIVwwnfXxwHGpRMlL_gPN9YFc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71672314</pqid></control><display><type>article</type><title>Calpain inhibition by cerebrospinal fluid and effects of calpain on intrathecal nerve tissue</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>KODAMA, Hirotaka ; SHIMIZU, Katsuji ; BANNO, Yoshiko ; NONOMURA, Yuka ; OSHITA, Hidefumi ; AKAIKE, Atsushi</creator><creatorcontrib>KODAMA, Hirotaka ; SHIMIZU, Katsuji ; BANNO, Yoshiko ; NONOMURA, Yuka ; OSHITA, Hidefumi ; AKAIKE, Atsushi</creatorcontrib><description>The effects of calpain on intrathecal nerve tissue in the rabbit were investigated.
To evaluate the chemonucleolytic side effect of calpain on nerve tissue in the event of accidental intrathecal calpain injection.
Calpain has a degradative effect on proteoglycans, and as previously shown, it is associated with chemonucleolytic action in the rabbit. However, its effect on nerve tissue in the event of accidental intrathecal injection is not clear.
The inhibitory activity of cerebrospinal fluid against calpain was measured in human cerebrospinal fluid using mu-calpain, and in different cerebrospinal fluid fractions separated by molecular filtration. The presence of the endogenous calpain inhibitor, calpastatin, in human cerebrospinal fluid was examined by Western blotting with anticalpastatin antibody. After intrathecal application of calpain in rabbits, the spinal cord nerve tissue was examined by light microscopy.
Cerebrospinal fluid inhibited the enzyme reaction of calpain at its normal concentration. Immunoblotting with anticalpastatin antibody did not yield positive staining. After the intrathecal application of calpain, there was no evidence of degeneration in the nerve tissue of the spinal cord.
This study suggests that in the event of accidental intrathecal injection of calpain for chemonucleolysis, the enzyme activity of calpain will be neutralized by cerebrospinal fluid, and the calpain should not cause unwanted side effects in chemonucleolysis.</description><identifier>ISSN: 0362-2436</identifier><identifier>EISSN: 1528-1159</identifier><identifier>DOI: 10.1097/00007632-200205150-00013</identifier><identifier>PMID: 12004175</identifier><identifier>CODEN: SPINDD</identifier><language>eng</language><publisher>Philadelphia, PA: Lippincott</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Biological and medical sciences ; Calpain - antagonists & inhibitors ; Calpain - metabolism ; Calpain - pharmacology ; Cerebrospinal Fluid - chemistry ; Cerebrospinal Fluid - physiology ; Diseases of the osteoarticular system. Orthopedic treatment ; Drug toxicity and drugs side effects treatment ; Female ; Humans ; Injections, Spinal ; Male ; Medical sciences ; Middle Aged ; Nerve Tissue - drug effects ; Nerve Tissue - pathology ; Pharmacology. Drug treatments ; Rabbits ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Spinal Cord - drug effects ; Spinal Cord - pathology ; Toxicity: nervous system and muscle</subject><ispartof>Spine (Philadelphia, Pa. 1976), 2002-05, Vol.27 (10), p.1077-1081</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-1a1e029b445d616eca985f59dc8e11b686c607cf57a62964c2d3e6bb74dc4e9f3</citedby><cites>FETCH-LOGICAL-c370t-1a1e029b445d616eca985f59dc8e11b686c607cf57a62964c2d3e6bb74dc4e9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13675838$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12004175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KODAMA, Hirotaka</creatorcontrib><creatorcontrib>SHIMIZU, Katsuji</creatorcontrib><creatorcontrib>BANNO, Yoshiko</creatorcontrib><creatorcontrib>NONOMURA, Yuka</creatorcontrib><creatorcontrib>OSHITA, Hidefumi</creatorcontrib><creatorcontrib>AKAIKE, Atsushi</creatorcontrib><title>Calpain inhibition by cerebrospinal fluid and effects of calpain on intrathecal nerve tissue</title><title>Spine (Philadelphia, Pa. 1976)</title><addtitle>Spine (Phila Pa 1976)</addtitle><description>The effects of calpain on intrathecal nerve tissue in the rabbit were investigated.
To evaluate the chemonucleolytic side effect of calpain on nerve tissue in the event of accidental intrathecal calpain injection.
Calpain has a degradative effect on proteoglycans, and as previously shown, it is associated with chemonucleolytic action in the rabbit. However, its effect on nerve tissue in the event of accidental intrathecal injection is not clear.
The inhibitory activity of cerebrospinal fluid against calpain was measured in human cerebrospinal fluid using mu-calpain, and in different cerebrospinal fluid fractions separated by molecular filtration. The presence of the endogenous calpain inhibitor, calpastatin, in human cerebrospinal fluid was examined by Western blotting with anticalpastatin antibody. After intrathecal application of calpain in rabbits, the spinal cord nerve tissue was examined by light microscopy.
Cerebrospinal fluid inhibited the enzyme reaction of calpain at its normal concentration. Immunoblotting with anticalpastatin antibody did not yield positive staining. After the intrathecal application of calpain, there was no evidence of degeneration in the nerve tissue of the spinal cord.
This study suggests that in the event of accidental intrathecal injection of calpain for chemonucleolysis, the enzyme activity of calpain will be neutralized by cerebrospinal fluid, and the calpain should not cause unwanted side effects in chemonucleolysis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calpain - antagonists & inhibitors</subject><subject>Calpain - metabolism</subject><subject>Calpain - pharmacology</subject><subject>Cerebrospinal Fluid - chemistry</subject><subject>Cerebrospinal Fluid - physiology</subject><subject>Diseases of the osteoarticular system. Orthopedic treatment</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Female</subject><subject>Humans</subject><subject>Injections, Spinal</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nerve Tissue - drug effects</subject><subject>Nerve Tissue - pathology</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - pathology</subject><subject>Toxicity: nervous system and muscle</subject><issn>0362-2436</issn><issn>1528-1159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF1LwzAUQIMobk7_guRF36q5TZO0jzL8goEv-iaENL1hka6dSSv4781cdXkJXM65IYcQCuwGWKVuWTpK8jzLGcuZAMGyNAF-ROYg8jIDENUxmTMuE1JwOSNnMX4kRHKoTskMkleAEnPyvjTt1viO-m7taz_4vqP1N7UYsA593PrOtNS1o2-o6RqKzqEdIu0dtZPY79whmGGNaUQ7DF9IBx_jiOfkxJk24sV0L8jbw_3r8ilbvTw-L-9WmeWKDRkYQJZXdVGIRoJMa6pSOFE1tkSAWpbSSqasE8rIvJKFzRuOsq5V0dgCK8cX5Hq_dxv6zxHjoDc-Wmxb02E_Rq1AqpxDkcByD9r0txjQ6W3wGxO-NTC9K6v_yur_svq3bFIvpzfGeoPNQZxSJuBqAkxMIVwwnfXxwHGpRMlL_gPN9YFc</recordid><startdate>20020515</startdate><enddate>20020515</enddate><creator>KODAMA, Hirotaka</creator><creator>SHIMIZU, Katsuji</creator><creator>BANNO, Yoshiko</creator><creator>NONOMURA, Yuka</creator><creator>OSHITA, Hidefumi</creator><creator>AKAIKE, Atsushi</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020515</creationdate><title>Calpain inhibition by cerebrospinal fluid and effects of calpain on intrathecal nerve tissue</title><author>KODAMA, Hirotaka ; SHIMIZU, Katsuji ; BANNO, Yoshiko ; NONOMURA, Yuka ; OSHITA, Hidefumi ; AKAIKE, Atsushi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-1a1e029b445d616eca985f59dc8e11b686c607cf57a62964c2d3e6bb74dc4e9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calpain - antagonists & inhibitors</topic><topic>Calpain - metabolism</topic><topic>Calpain - pharmacology</topic><topic>Cerebrospinal Fluid - chemistry</topic><topic>Cerebrospinal Fluid - physiology</topic><topic>Diseases of the osteoarticular system. Orthopedic treatment</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Female</topic><topic>Humans</topic><topic>Injections, Spinal</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Nerve Tissue - drug effects</topic><topic>Nerve Tissue - pathology</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - pathology</topic><topic>Toxicity: nervous system and muscle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KODAMA, Hirotaka</creatorcontrib><creatorcontrib>SHIMIZU, Katsuji</creatorcontrib><creatorcontrib>BANNO, Yoshiko</creatorcontrib><creatorcontrib>NONOMURA, Yuka</creatorcontrib><creatorcontrib>OSHITA, Hidefumi</creatorcontrib><creatorcontrib>AKAIKE, Atsushi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KODAMA, Hirotaka</au><au>SHIMIZU, Katsuji</au><au>BANNO, Yoshiko</au><au>NONOMURA, Yuka</au><au>OSHITA, Hidefumi</au><au>AKAIKE, Atsushi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calpain inhibition by cerebrospinal fluid and effects of calpain on intrathecal nerve tissue</atitle><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle><addtitle>Spine (Phila Pa 1976)</addtitle><date>2002-05-15</date><risdate>2002</risdate><volume>27</volume><issue>10</issue><spage>1077</spage><epage>1081</epage><pages>1077-1081</pages><issn>0362-2436</issn><eissn>1528-1159</eissn><coden>SPINDD</coden><abstract>The effects of calpain on intrathecal nerve tissue in the rabbit were investigated.
To evaluate the chemonucleolytic side effect of calpain on nerve tissue in the event of accidental intrathecal calpain injection.
Calpain has a degradative effect on proteoglycans, and as previously shown, it is associated with chemonucleolytic action in the rabbit. However, its effect on nerve tissue in the event of accidental intrathecal injection is not clear.
The inhibitory activity of cerebrospinal fluid against calpain was measured in human cerebrospinal fluid using mu-calpain, and in different cerebrospinal fluid fractions separated by molecular filtration. The presence of the endogenous calpain inhibitor, calpastatin, in human cerebrospinal fluid was examined by Western blotting with anticalpastatin antibody. After intrathecal application of calpain in rabbits, the spinal cord nerve tissue was examined by light microscopy.
Cerebrospinal fluid inhibited the enzyme reaction of calpain at its normal concentration. Immunoblotting with anticalpastatin antibody did not yield positive staining. After the intrathecal application of calpain, there was no evidence of degeneration in the nerve tissue of the spinal cord.
This study suggests that in the event of accidental intrathecal injection of calpain for chemonucleolysis, the enzyme activity of calpain will be neutralized by cerebrospinal fluid, and the calpain should not cause unwanted side effects in chemonucleolysis.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>12004175</pmid><doi>10.1097/00007632-200205150-00013</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0362-2436 |
| ispartof | Spine (Philadelphia, Pa. 1976), 2002-05, Vol.27 (10), p.1077-1081 |
| issn | 0362-2436 1528-1159 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71672314 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adolescent Adult Aged Aged, 80 and over Animals Biological and medical sciences Calpain - antagonists & inhibitors Calpain - metabolism Calpain - pharmacology Cerebrospinal Fluid - chemistry Cerebrospinal Fluid - physiology Diseases of the osteoarticular system. Orthopedic treatment Drug toxicity and drugs side effects treatment Female Humans Injections, Spinal Male Medical sciences Middle Aged Nerve Tissue - drug effects Nerve Tissue - pathology Pharmacology. Drug treatments Rabbits Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Spinal Cord - drug effects Spinal Cord - pathology Toxicity: nervous system and muscle |
| title | Calpain inhibition by cerebrospinal fluid and effects of calpain on intrathecal nerve tissue |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T01%3A49%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Calpain%20inhibition%20by%20cerebrospinal%20fluid%20and%20effects%20of%20calpain%20on%20intrathecal%20nerve%20tissue&rft.jtitle=Spine%20(Philadelphia,%20Pa.%201976)&rft.au=KODAMA,%20Hirotaka&rft.date=2002-05-15&rft.volume=27&rft.issue=10&rft.spage=1077&rft.epage=1081&rft.pages=1077-1081&rft.issn=0362-2436&rft.eissn=1528-1159&rft.coden=SPINDD&rft_id=info:doi/10.1097/00007632-200205150-00013&rft_dat=%3Cproquest_cross%3E71672314%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71672314&rft_id=info:pmid/12004175&rfr_iscdi=true |