RGS5 expression is a quantitative measure of pericyte coverage of blood vessels
Pericytes play a key role in the process of vascular maturation and stabilization however, the current methods for quantifying pericyte coverage of the neovasculature are laborious and subjective in nature. In this study, we have developed an objective, sensitive, and high-throughput method for quan...
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| Veröffentlicht in: | Angiogenesis (London) 2008-06, Vol.11 (2), p.141-151 |
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| creator | Mitchell, Tracy S. Bradley, John Robinson, Gregory S. Shima, David T. Ng, Yin-Shan |
| description | Pericytes play a key role in the process of vascular maturation and stabilization however, the current methods for quantifying pericyte coverage of the neovasculature are laborious and subjective in nature. In this study, we have developed an objective, sensitive, and high-throughput method for quantifying pericyte coverage of angiogenic vessels by analyzing the expression of the pericyte-specific gene, the regulator of G-protein signaling 5 (RGS5). We determined that RGS5 expression was up-regulated during a defined developmental time period in which nascent vessel sprouts acquired a pericyte covering. Furthermore, RGS5 expression was dramatically reduced in vessels with poor pericyte coverage compared to normal angiogenic vasculature. Finally, we determined that the susceptibility of nascent vessels to regression by vascular endothelial growth factor (VEGF) inhibition was significantly reduced following RGS5 up-regulation, further implicating RGS5 in pericyte-endothelial cell interactions and the vascular maturation process. These studies establish the use of RGS5 gene expression as a quantitative and robust measure of pericyte coverage of neovasculature. This method provides a tool for vascular biologists studying pericyte-endothelial cell interactions and vascular maturation in both normal and pathological conditions, such as diabetic retinopathy and cancer. |
| doi_str_mv | 10.1007/s10456-007-9085-x |
| format | Article |
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In this study, we have developed an objective, sensitive, and high-throughput method for quantifying pericyte coverage of angiogenic vessels by analyzing the expression of the pericyte-specific gene, the regulator of G-protein signaling 5 (RGS5). We determined that RGS5 expression was up-regulated during a defined developmental time period in which nascent vessel sprouts acquired a pericyte covering. Furthermore, RGS5 expression was dramatically reduced in vessels with poor pericyte coverage compared to normal angiogenic vasculature. Finally, we determined that the susceptibility of nascent vessels to regression by vascular endothelial growth factor (VEGF) inhibition was significantly reduced following RGS5 up-regulation, further implicating RGS5 in pericyte-endothelial cell interactions and the vascular maturation process. These studies establish the use of RGS5 gene expression as a quantitative and robust measure of pericyte coverage of neovasculature. This method provides a tool for vascular biologists studying pericyte-endothelial cell interactions and vascular maturation in both normal and pathological conditions, such as diabetic retinopathy and cancer.</description><identifier>ISSN: 0969-6970</identifier><identifier>EISSN: 1573-7209</identifier><identifier>DOI: 10.1007/s10456-007-9085-x</identifier><identifier>PMID: 18038251</identifier><identifier>CODEN: AGIOFT</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Aptamers, Nucleotide - pharmacology ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Blood and lymphatic vessels ; Brain ; Cancer Research ; Cardiology ; Cardiology. Vascular system ; Cell Biology ; Cornea - blood supply ; Cornea - drug effects ; Cornea - pathology ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Gene Expression Regulation - drug effects ; Investigative techniques of hemodynamics ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Neovascularization, Physiologic - genetics ; Oncology ; Ophthalmology ; Original Paper ; Pericytes - drug effects ; Pericytes - metabolism ; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism ; Platelet-Derived Growth Factor - antagonists & inhibitors ; Retina - drug effects ; Retina - embryology ; Retina - pathology ; RGS Proteins - genetics ; RGS Proteins - metabolism ; Vascular Endothelial Growth Factor A - pharmacology</subject><ispartof>Angiogenesis (London), 2008-06, Vol.11 (2), p.141-151</ispartof><rights>Springer Science+Business Media B.V. 2007</rights><rights>2008 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-3a0f1b3aa42df119e6b44e1b2997ae3ff7b835be904dd114bdb205c45c18b8a23</citedby><cites>FETCH-LOGICAL-c399t-3a0f1b3aa42df119e6b44e1b2997ae3ff7b835be904dd114bdb205c45c18b8a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10456-007-9085-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10456-007-9085-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20453452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18038251$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitchell, Tracy S.</creatorcontrib><creatorcontrib>Bradley, John</creatorcontrib><creatorcontrib>Robinson, Gregory S.</creatorcontrib><creatorcontrib>Shima, David T.</creatorcontrib><creatorcontrib>Ng, Yin-Shan</creatorcontrib><title>RGS5 expression is a quantitative measure of pericyte coverage of blood vessels</title><title>Angiogenesis (London)</title><addtitle>Angiogenesis</addtitle><addtitle>Angiogenesis</addtitle><description>Pericytes play a key role in the process of vascular maturation and stabilization however, the current methods for quantifying pericyte coverage of the neovasculature are laborious and subjective in nature. 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This method provides a tool for vascular biologists studying pericyte-endothelial cell interactions and vascular maturation in both normal and pathological conditions, such as diabetic retinopathy and cancer.</description><subject>Animals</subject><subject>Aptamers, Nucleotide - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood and lymphatic vessels</subject><subject>Brain</subject><subject>Cancer Research</subject><subject>Cardiology</subject><subject>Cardiology. Vascular system</subject><subject>Cell Biology</subject><subject>Cornea - blood supply</subject><subject>Cornea - drug effects</subject><subject>Cornea - pathology</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. 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Vascular system</topic><topic>Cell Biology</topic><topic>Cornea - blood supply</topic><topic>Cornea - drug effects</topic><topic>Cornea - pathology</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Investigative techniques of hemodynamics</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neovascularization, Physiologic - genetics</topic><topic>Oncology</topic><topic>Ophthalmology</topic><topic>Original Paper</topic><topic>Pericytes - drug effects</topic><topic>Pericytes - metabolism</topic><topic>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</topic><topic>Platelet-Derived Growth Factor - antagonists & inhibitors</topic><topic>Retina - drug effects</topic><topic>Retina - embryology</topic><topic>Retina - pathology</topic><topic>RGS Proteins - genetics</topic><topic>RGS Proteins - metabolism</topic><topic>Vascular Endothelial Growth Factor A - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitchell, Tracy S.</creatorcontrib><creatorcontrib>Bradley, John</creatorcontrib><creatorcontrib>Robinson, Gregory S.</creatorcontrib><creatorcontrib>Shima, David T.</creatorcontrib><creatorcontrib>Ng, Yin-Shan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Angiogenesis (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitchell, Tracy S.</au><au>Bradley, John</au><au>Robinson, Gregory S.</au><au>Shima, David T.</au><au>Ng, Yin-Shan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RGS5 expression is a quantitative measure of pericyte coverage of blood vessels</atitle><jtitle>Angiogenesis (London)</jtitle><stitle>Angiogenesis</stitle><addtitle>Angiogenesis</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>11</volume><issue>2</issue><spage>141</spage><epage>151</epage><pages>141-151</pages><issn>0969-6970</issn><eissn>1573-7209</eissn><coden>AGIOFT</coden><abstract>Pericytes play a key role in the process of vascular maturation and stabilization however, the current methods for quantifying pericyte coverage of the neovasculature are laborious and subjective in nature. In this study, we have developed an objective, sensitive, and high-throughput method for quantifying pericyte coverage of angiogenic vessels by analyzing the expression of the pericyte-specific gene, the regulator of G-protein signaling 5 (RGS5). We determined that RGS5 expression was up-regulated during a defined developmental time period in which nascent vessel sprouts acquired a pericyte covering. Furthermore, RGS5 expression was dramatically reduced in vessels with poor pericyte coverage compared to normal angiogenic vasculature. Finally, we determined that the susceptibility of nascent vessels to regression by vascular endothelial growth factor (VEGF) inhibition was significantly reduced following RGS5 up-regulation, further implicating RGS5 in pericyte-endothelial cell interactions and the vascular maturation process. These studies establish the use of RGS5 gene expression as a quantitative and robust measure of pericyte coverage of neovasculature. This method provides a tool for vascular biologists studying pericyte-endothelial cell interactions and vascular maturation in both normal and pathological conditions, such as diabetic retinopathy and cancer.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>18038251</pmid><doi>10.1007/s10456-007-9085-x</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Aptamers, Nucleotide - pharmacology Biological and medical sciences Biomedical and Life Sciences Biomedicine Blood and lymphatic vessels Brain Cancer Research Cardiology Cardiology. Vascular system Cell Biology Cornea - blood supply Cornea - drug effects Cornea - pathology Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Gene Expression Regulation - drug effects Investigative techniques of hemodynamics Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Mice Mice, Inbred C57BL Neovascularization, Physiologic - genetics Oncology Ophthalmology Original Paper Pericytes - drug effects Pericytes - metabolism Platelet Endothelial Cell Adhesion Molecule-1 - metabolism Platelet-Derived Growth Factor - antagonists & inhibitors Retina - drug effects Retina - embryology Retina - pathology RGS Proteins - genetics RGS Proteins - metabolism Vascular Endothelial Growth Factor A - pharmacology |
| title | RGS5 expression is a quantitative measure of pericyte coverage of blood vessels |
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