Determination of omeprazole and its metabolites in human plasma by liquid chromatography–mass spectrometry
Omeprazole is a benzimidazole compound that acts as a proton-pump inhibitor. Because the metabolism of omeprazole is mainly catalyzed by cytochrome P-450 ( CYP) 3A4 and CYP2C19, the genetic polymorphism of CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole...
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| Veröffentlicht in: | Journal of Chromatography A 2002-03, Vol.949 (1), p.1-9 |
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| creator | Kanazawa, Hideko Okada, Akiko Matsushima, Yoshikazu Yokota, Hiromitsu Okubo, Shigeo Mashige, Fumiko Nakahara, Kazuhiko |
| description | Omeprazole is a benzimidazole compound that acts as a proton-pump inhibitor. Because the metabolism of omeprazole is mainly catalyzed by cytochrome P-450 (
CYP)
3A4 and
CYP2C19, the genetic polymorphism of
CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole. Therefore, a reliable method for omeprazole phenotyping is desirable in clinical situations. This study has demonstrated the determination of omeprazole and its metabolites in human plasma by liquid chromatography–three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro C
18(50×2.0 mm I.D.) using acetonitrile–50 m
M ammonium acetate (pH 7.25) (1:4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110 °C and Shield temperature was 230 °C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole and omeprazole sulfone were clearly observed as base peaks. This method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazol. |
| doi_str_mv | 10.1016/S0021-9673(01)01508-4 |
| format | Article |
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CYP)
3A4 and
CYP2C19, the genetic polymorphism of
CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole. Therefore, a reliable method for omeprazole phenotyping is desirable in clinical situations. This study has demonstrated the determination of omeprazole and its metabolites in human plasma by liquid chromatography–three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro C
18(50×2.0 mm I.D.) using acetonitrile–50 m
M ammonium acetate (pH 7.25) (1:4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110 °C and Shield temperature was 230 °C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole and omeprazole sulfone were clearly observed as base peaks. This method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazol.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(01)01508-4</identifier><identifier>PMID: 11999727</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Anti-Ulcer Agents - blood ; Anti-Ulcer Agents - pharmacokinetics ; Aryl Hydrocarbon Hydroxylases - genetics ; Aryl Hydrocarbon Hydroxylases - metabolism ; Benzimidazoles ; Biological and medical sciences ; Calibration ; Chromatography, Liquid - methods ; Cytochrome P-450 CYP2C19 ; Cytochromes ; Female ; General pharmacology ; Humans ; Mass Spectrometry - methods ; Medical sciences ; Mixed Function Oxygenases - genetics ; Mixed Function Oxygenases - metabolism ; Omeprazole ; Omeprazole - blood ; Omeprazole - pharmacokinetics ; Pharmacology. Drug treatments ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Reproducibility of Results</subject><ispartof>Journal of Chromatography A, 2002-03, Vol.949 (1), p.1-9</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-df532eefe764ec498f3662d8ec0562be483ed5ef0870745893b24b4949832acc3</citedby><cites>FETCH-LOGICAL-c391t-df532eefe764ec498f3662d8ec0562be483ed5ef0870745893b24b4949832acc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021967301015084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3536,23910,23911,25119,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13543907$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11999727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Okada, Akiko</creatorcontrib><creatorcontrib>Matsushima, Yoshikazu</creatorcontrib><creatorcontrib>Yokota, Hiromitsu</creatorcontrib><creatorcontrib>Okubo, Shigeo</creatorcontrib><creatorcontrib>Mashige, Fumiko</creatorcontrib><creatorcontrib>Nakahara, Kazuhiko</creatorcontrib><title>Determination of omeprazole and its metabolites in human plasma by liquid chromatography–mass spectrometry</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>Omeprazole is a benzimidazole compound that acts as a proton-pump inhibitor. Because the metabolism of omeprazole is mainly catalyzed by cytochrome P-450 (
CYP)
3A4 and
CYP2C19, the genetic polymorphism of
CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole. Therefore, a reliable method for omeprazole phenotyping is desirable in clinical situations. This study has demonstrated the determination of omeprazole and its metabolites in human plasma by liquid chromatography–three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro C
18(50×2.0 mm I.D.) using acetonitrile–50 m
M ammonium acetate (pH 7.25) (1:4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110 °C and Shield temperature was 230 °C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole and omeprazole sulfone were clearly observed as base peaks. This method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazol.</description><subject>Analysis</subject><subject>Anti-Ulcer Agents - blood</subject><subject>Anti-Ulcer Agents - pharmacokinetics</subject><subject>Aryl Hydrocarbon Hydroxylases - genetics</subject><subject>Aryl Hydrocarbon Hydroxylases - metabolism</subject><subject>Benzimidazoles</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chromatography, Liquid - methods</subject><subject>Cytochrome P-450 CYP2C19</subject><subject>Cytochromes</subject><subject>Female</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Mixed Function Oxygenases - genetics</subject><subject>Mixed Function Oxygenases - metabolism</subject><subject>Omeprazole</subject><subject>Omeprazole - blood</subject><subject>Omeprazole - pharmacokinetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Reproducibility of Results</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1DAQgH0ooqX0Eah8KYJDqB07TnxCqPxKlTi0nK2JM2GN7Di1HaTlxDvwhjwJ2e6qPXIaaeabv4-QF5y94YyryxvGal5p1YpXjL9mvGFdJY_IyUP6mDzL-QdjvGVt_ZQcc661buv2hPj3WDAFN0FxcaJxpDHgnOBX9EhhGqgrmQYs0EfvCmbqJrpZAkx09pAD0H5Lvbtb3EDtJsUAJX5PMG-2f3__CZAzzTPashawpO1z8mQEn_HsEE_Jt48fbq8-V9dfP325enddWaF5qYaxETXiiK2SaKXuRqFUPXRoWaPqHmUncGhwZN36jmw6Lfpa9lKvpKjBWnFKXu7nzineLZiLCS5b9B4mjEs2LVdKdZqtYLMHbYo5JxzNnFyAtDWcmZ1ac6_W7Bwaxs29WiPXvvPDgqUPODx2HbyuwMUBgGzBjwkm6_IjJxopNNtxb_ccrjp-OkwmW4eTxcGl1ZsZovvPKf8ABWSa4w</recordid><startdate>20020308</startdate><enddate>20020308</enddate><creator>Kanazawa, Hideko</creator><creator>Okada, Akiko</creator><creator>Matsushima, Yoshikazu</creator><creator>Yokota, Hiromitsu</creator><creator>Okubo, Shigeo</creator><creator>Mashige, Fumiko</creator><creator>Nakahara, Kazuhiko</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020308</creationdate><title>Determination of omeprazole and its metabolites in human plasma by liquid chromatography–mass spectrometry</title><author>Kanazawa, Hideko ; Okada, Akiko ; Matsushima, Yoshikazu ; Yokota, Hiromitsu ; Okubo, Shigeo ; Mashige, Fumiko ; Nakahara, Kazuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-df532eefe764ec498f3662d8ec0562be483ed5ef0870745893b24b4949832acc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analysis</topic><topic>Anti-Ulcer Agents - blood</topic><topic>Anti-Ulcer Agents - pharmacokinetics</topic><topic>Aryl Hydrocarbon Hydroxylases - genetics</topic><topic>Aryl Hydrocarbon Hydroxylases - metabolism</topic><topic>Benzimidazoles</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chromatography, Liquid - methods</topic><topic>Cytochrome P-450 CYP2C19</topic><topic>Cytochromes</topic><topic>Female</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Mixed Function Oxygenases - genetics</topic><topic>Mixed Function Oxygenases - metabolism</topic><topic>Omeprazole</topic><topic>Omeprazole - blood</topic><topic>Omeprazole - pharmacokinetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Okada, Akiko</creatorcontrib><creatorcontrib>Matsushima, Yoshikazu</creatorcontrib><creatorcontrib>Yokota, Hiromitsu</creatorcontrib><creatorcontrib>Okubo, Shigeo</creatorcontrib><creatorcontrib>Mashige, Fumiko</creatorcontrib><creatorcontrib>Nakahara, Kazuhiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanazawa, Hideko</au><au>Okada, Akiko</au><au>Matsushima, Yoshikazu</au><au>Yokota, Hiromitsu</au><au>Okubo, Shigeo</au><au>Mashige, Fumiko</au><au>Nakahara, Kazuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of omeprazole and its metabolites in human plasma by liquid chromatography–mass spectrometry</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2002-03-08</date><risdate>2002</risdate><volume>949</volume><issue>1</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>Omeprazole is a benzimidazole compound that acts as a proton-pump inhibitor. Because the metabolism of omeprazole is mainly catalyzed by cytochrome P-450 (
CYP)
3A4 and
CYP2C19, the genetic polymorphism of
CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole. Therefore, a reliable method for omeprazole phenotyping is desirable in clinical situations. This study has demonstrated the determination of omeprazole and its metabolites in human plasma by liquid chromatography–three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro C
18(50×2.0 mm I.D.) using acetonitrile–50 m
M ammonium acetate (pH 7.25) (1:4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110 °C and Shield temperature was 230 °C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole and omeprazole sulfone were clearly observed as base peaks. This method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazol.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11999727</pmid><doi>10.1016/S0021-9673(01)01508-4</doi><tpages>9</tpages></addata></record> |
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| subjects | Analysis Anti-Ulcer Agents - blood Anti-Ulcer Agents - pharmacokinetics Aryl Hydrocarbon Hydroxylases - genetics Aryl Hydrocarbon Hydroxylases - metabolism Benzimidazoles Biological and medical sciences Calibration Chromatography, Liquid - methods Cytochrome P-450 CYP2C19 Cytochromes Female General pharmacology Humans Mass Spectrometry - methods Medical sciences Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Omeprazole Omeprazole - blood Omeprazole - pharmacokinetics Pharmacology. Drug treatments Recombinant Proteins - genetics Recombinant Proteins - metabolism Reproducibility of Results |
| title | Determination of omeprazole and its metabolites in human plasma by liquid chromatography–mass spectrometry |
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