Immobilization of Penicillin G Acylase on Poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-Grafted Magnetic Microspheres
Poly[(glycidyl methacrylate)‐co‐(glycerol monomethacrylate)]‐grafted magnetic microspheres were prepared by graft random copolymerization via ATRP from polymer microspheres with dispersed Fe3O4 nanoparticles. Penicillin G acylase (PGA) was immobilized onto the polymer brush‐grafted magnetic microsph...
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| Veröffentlicht in: | Macromolecular bioscience 2008-06, Vol.8 (6), p.508-515 |
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| creator | Huang, Junsheng Li, Xiutao Zheng, Yanhui Zhang, Yong Zhao, Ruiying Gao, Xicai Yan, Husheng |
| description | Poly[(glycidyl methacrylate)‐co‐(glycerol monomethacrylate)]‐grafted magnetic microspheres were prepared by graft random copolymerization via ATRP from polymer microspheres with dispersed Fe3O4 nanoparticles. Penicillin G acylase (PGA) was immobilized onto the polymer brush‐grafted magnetic microspheres. The immobilized PGA prepared with initial glycidyl methacrylate/ glycerol monomethacrylate ratios of 40/60 to 60/40 possessed higher catalytic activity than that prepared with higher proportions of glycidyl methacrylate in the initial monomer mixture. The immobilized PGA showed high thermal stability and enhanced tolerability to the pH variance. |
| doi_str_mv | 10.1002/mabi.200700256 |
| format | Article |
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Penicillin G acylase (PGA) was immobilized onto the polymer brush‐grafted magnetic microspheres. The immobilized PGA prepared with initial glycidyl methacrylate/ glycerol monomethacrylate ratios of 40/60 to 60/40 possessed higher catalytic activity than that prepared with higher proportions of glycidyl methacrylate in the initial monomer mixture. The immobilized PGA showed high thermal stability and enhanced tolerability to the pH variance.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.200700256</identifier><identifier>PMID: 18322908</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Applied sciences ; Biological and medical sciences ; Biotechnology ; Catalysis ; enzyme immobilization ; Enzyme Stability ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Epoxy Compounds - chemistry ; Exact sciences and technology ; Ferric Compounds - chemistry ; Ferrous Compounds - chemistry ; Fundamental and applied biological sciences. Psychology ; graft copolymers ; Hydrogen-Ion Concentration ; Immobilization of enzymes and other molecules ; Immobilization techniques ; magnetic microbeads ; magnetic polymers ; Magnetics ; Methacrylates - chemical synthesis ; Methacrylates - chemistry ; Methods. Procedures. Technologies ; Nanoparticles - chemistry ; Organic polymers ; Osmolar Concentration ; Penicillin Amidase - chemistry ; Penicillin Amidase - metabolism ; penicillin G acylase ; Physicochemistry of polymers ; Polymers with particular properties ; Polymethacrylic Acids - chemistry ; Preparation, kinetics, thermodynamics, mechanism and catalysts ; Temperature</subject><ispartof>Macromolecular bioscience, 2008-06, Vol.8 (6), p.508-515</ispartof><rights>Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. 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Biosci</addtitle><description>Poly[(glycidyl methacrylate)‐co‐(glycerol monomethacrylate)]‐grafted magnetic microspheres were prepared by graft random copolymerization via ATRP from polymer microspheres with dispersed Fe3O4 nanoparticles. Penicillin G acylase (PGA) was immobilized onto the polymer brush‐grafted magnetic microspheres. The immobilized PGA prepared with initial glycidyl methacrylate/ glycerol monomethacrylate ratios of 40/60 to 60/40 possessed higher catalytic activity than that prepared with higher proportions of glycidyl methacrylate in the initial monomer mixture. The immobilized PGA showed high thermal stability and enhanced tolerability to the pH variance.</description><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Catalysis</subject><subject>enzyme immobilization</subject><subject>Enzyme Stability</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Epoxy Compounds - chemistry</subject><subject>Exact sciences and technology</subject><subject>Ferric Compounds - chemistry</subject><subject>Ferrous Compounds - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>graft copolymers</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immobilization of enzymes and other molecules</subject><subject>Immobilization techniques</subject><subject>magnetic microbeads</subject><subject>magnetic polymers</subject><subject>Magnetics</subject><subject>Methacrylates - chemical synthesis</subject><subject>Methacrylates - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Nanoparticles - chemistry</subject><subject>Organic polymers</subject><subject>Osmolar Concentration</subject><subject>Penicillin Amidase - chemistry</subject><subject>Penicillin Amidase - metabolism</subject><subject>penicillin G acylase</subject><subject>Physicochemistry of polymers</subject><subject>Polymers with particular properties</subject><subject>Polymethacrylic Acids - chemistry</subject><subject>Preparation, kinetics, thermodynamics, mechanism and catalysts</subject><subject>Temperature</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9v0zAYhiMEYmNw5YhyAcEhxT9iOz52E3QTLUwCtANC1hfny2Zw4mKngnDmDyelVYHTTrb1PO9nW2-WPaZkRglhLzuo3YwRoqaDkHeyYyqpLATV4u5hX6mj7EFKXwihqtLsfnZEK86YJtVx9uui60LtvPsJgwt9Htr8Entnnfeuzxf53I4eEuYTugx-_PT82o_WNaPPOxxuwMYJD_iisKH4gzCGCYU-_Ic_F4sI7YBNvoLrHgdn85WzMaT1DUZMD7N7LfiEj_brSfbx9asPZ-fF8t3i4my-LGxZMllY3rQt1FToGigwgIZaVKWoNSfQCNAorWi41ZxygVhXJXCKJSpLKtYg4yfZs93cdQzfNpgG07lk0XvoMWySUVTKspL6VpFzLrgWdBJnO3H7mRSxNevoOoijocRsCzLbgsyhoCnwZD95U3fY_NX3jUzC070AyYJvI_TWpYPHSEloqcTk6Z333Xkcb7nWrOanF_8-othlXRrwxyEL8auRiithrt4uzPmpuirfvJdmyX8DYp-76Q</recordid><startdate>20080611</startdate><enddate>20080611</enddate><creator>Huang, Junsheng</creator><creator>Li, Xiutao</creator><creator>Zheng, Yanhui</creator><creator>Zhang, Yong</creator><creator>Zhao, Ruiying</creator><creator>Gao, Xicai</creator><creator>Yan, Husheng</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20080611</creationdate><title>Immobilization of Penicillin G Acylase on Poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-Grafted Magnetic Microspheres</title><author>Huang, Junsheng ; Li, Xiutao ; Zheng, Yanhui ; Zhang, Yong ; Zhao, Ruiying ; Gao, Xicai ; Yan, Husheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4426-c3dffab159ba1a2aad1ce745b930ad5a9e6c5d3c93135eeb84a31e4e7c082de23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Catalysis</topic><topic>enzyme immobilization</topic><topic>Enzyme Stability</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Epoxy Compounds - chemistry</topic><topic>Exact sciences and technology</topic><topic>Ferric Compounds - chemistry</topic><topic>Ferrous Compounds - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>graft copolymers</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immobilization of enzymes and other molecules</topic><topic>Immobilization techniques</topic><topic>magnetic microbeads</topic><topic>magnetic polymers</topic><topic>Magnetics</topic><topic>Methacrylates - chemical synthesis</topic><topic>Methacrylates - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Nanoparticles - chemistry</topic><topic>Organic polymers</topic><topic>Osmolar Concentration</topic><topic>Penicillin Amidase - chemistry</topic><topic>Penicillin Amidase - metabolism</topic><topic>penicillin G acylase</topic><topic>Physicochemistry of polymers</topic><topic>Polymers with particular properties</topic><topic>Polymethacrylic Acids - chemistry</topic><topic>Preparation, kinetics, thermodynamics, mechanism and catalysts</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Junsheng</creatorcontrib><creatorcontrib>Li, Xiutao</creatorcontrib><creatorcontrib>Zheng, Yanhui</creatorcontrib><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Zhao, Ruiying</creatorcontrib><creatorcontrib>Gao, Xicai</creatorcontrib><creatorcontrib>Yan, Husheng</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Junsheng</au><au>Li, Xiutao</au><au>Zheng, Yanhui</au><au>Zhang, Yong</au><au>Zhao, Ruiying</au><au>Gao, Xicai</au><au>Yan, Husheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of Penicillin G Acylase on Poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-Grafted Magnetic Microspheres</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2008-06-11</date><risdate>2008</risdate><volume>8</volume><issue>6</issue><spage>508</spage><epage>515</epage><pages>508-515</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>Poly[(glycidyl methacrylate)‐co‐(glycerol monomethacrylate)]‐grafted magnetic microspheres were prepared by graft random copolymerization via ATRP from polymer microspheres with dispersed Fe3O4 nanoparticles. Penicillin G acylase (PGA) was immobilized onto the polymer brush‐grafted magnetic microspheres. The immobilized PGA prepared with initial glycidyl methacrylate/ glycerol monomethacrylate ratios of 40/60 to 60/40 possessed higher catalytic activity than that prepared with higher proportions of glycidyl methacrylate in the initial monomer mixture. The immobilized PGA showed high thermal stability and enhanced tolerability to the pH variance.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>18322908</pmid><doi>10.1002/mabi.200700256</doi><tpages>8</tpages></addata></record> |
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| ispartof | Macromolecular bioscience, 2008-06, Vol.8 (6), p.508-515 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Applied sciences Biological and medical sciences Biotechnology Catalysis enzyme immobilization Enzyme Stability Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Epoxy Compounds - chemistry Exact sciences and technology Ferric Compounds - chemistry Ferrous Compounds - chemistry Fundamental and applied biological sciences. Psychology graft copolymers Hydrogen-Ion Concentration Immobilization of enzymes and other molecules Immobilization techniques magnetic microbeads magnetic polymers Magnetics Methacrylates - chemical synthesis Methacrylates - chemistry Methods. Procedures. Technologies Nanoparticles - chemistry Organic polymers Osmolar Concentration Penicillin Amidase - chemistry Penicillin Amidase - metabolism penicillin G acylase Physicochemistry of polymers Polymers with particular properties Polymethacrylic Acids - chemistry Preparation, kinetics, thermodynamics, mechanism and catalysts Temperature |
| title | Immobilization of Penicillin G Acylase on Poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-Grafted Magnetic Microspheres |
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