Follicle-stimulating hormone receptors in oocytes?
The regulatory mechanisms of oocyte maturation remain poorly understood. Although gonadotropins play a major role in these processes, they have generally been considered to act on somatic supportive cells, but not directly on germ cells. We have raised high affinity monoclonal antibodies against LH...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2002-05, Vol.87 (5), p.2266-2276 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | MEDURI, Geri CHARNAUX, Nathalie DRIANCOURT, Marc-Antoine COMBETTES, Laurent GRANET, Philippe VANNIER, Brigitte LOOSFELT, Hugues MILGROM, Edwin |
| description | The regulatory mechanisms of oocyte maturation remain poorly understood. Although gonadotropins play a major role in these processes, they have generally been considered to act on somatic supportive cells, but not directly on germ cells. We have raised high affinity monoclonal antibodies against LH and FSH receptors. When using the latter to study receptor distribution in human and pig ovaries we have observed the presence of FSH (but not LH) receptors in the oocytes. FSH receptors appeared in the oocytes of primary follicles during follicular development and persisted up to the preovulatory stage. In denuded human preovulatory oocytes, FSH receptor mRNA was detected at a concentration per cell exceeding by about 20-fold that present in granulosa cells. Saturable binding of [(125)I]FSH to the membrane of oocytes was demonstrated by autoradiography. When incubated with FSH, denuded oocytes responded by a mobilization of Ca(2+). These observations concur to demonstrate the presence of functional FSH receptors in oocytes and raise the possibility of direct control of oocyte development by FSH. |
| doi_str_mv | 10.1210/jc.87.5.2266 |
| format | Article |
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Although gonadotropins play a major role in these processes, they have generally been considered to act on somatic supportive cells, but not directly on germ cells. We have raised high affinity monoclonal antibodies against LH and FSH receptors. When using the latter to study receptor distribution in human and pig ovaries we have observed the presence of FSH (but not LH) receptors in the oocytes. FSH receptors appeared in the oocytes of primary follicles during follicular development and persisted up to the preovulatory stage. In denuded human preovulatory oocytes, FSH receptor mRNA was detected at a concentration per cell exceeding by about 20-fold that present in granulosa cells. Saturable binding of [(125)I]FSH to the membrane of oocytes was demonstrated by autoradiography. When incubated with FSH, denuded oocytes responded by a mobilization of Ca(2+). These observations concur to demonstrate the presence of functional FSH receptors in oocytes and raise the possibility of direct control of oocyte development by FSH.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.87.5.2266</identifier><identifier>PMID: 11994374</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Adult ; Animals ; Autoradiography ; Biological and medical sciences ; Biological Transport - drug effects ; Calcium - metabolism ; Endocrinopathies ; Female ; Follicle Stimulating Hormone - metabolism ; Follicular Atresia - metabolism ; Hormones. Endocrine system ; Humans ; Immunohistochemistry - methods ; Malignant tumors ; Medical sciences ; Oocytes - metabolism ; Ovarian Follicle - cytology ; Ovarian Follicle - physiology ; Pharmacology. Drug treatments ; Receptors, FSH - genetics ; Receptors, FSH - metabolism ; RNA, Messenger - metabolism ; Staining and Labeling ; Swine ; Thyroid. Thyroid axis (diseases)</subject><ispartof>The journal of clinical endocrinology and metabolism, 2002-05, Vol.87 (5), p.2266-2276</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-41bc1364143a1563b759e2172f185875b387a94a7c3b2d934b30e7eee20898473</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13665716$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11994374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MEDURI, Geri</creatorcontrib><creatorcontrib>CHARNAUX, Nathalie</creatorcontrib><creatorcontrib>DRIANCOURT, Marc-Antoine</creatorcontrib><creatorcontrib>COMBETTES, Laurent</creatorcontrib><creatorcontrib>GRANET, Philippe</creatorcontrib><creatorcontrib>VANNIER, Brigitte</creatorcontrib><creatorcontrib>LOOSFELT, Hugues</creatorcontrib><creatorcontrib>MILGROM, Edwin</creatorcontrib><title>Follicle-stimulating hormone receptors in oocytes?</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>The regulatory mechanisms of oocyte maturation remain poorly understood. Although gonadotropins play a major role in these processes, they have generally been considered to act on somatic supportive cells, but not directly on germ cells. We have raised high affinity monoclonal antibodies against LH and FSH receptors. When using the latter to study receptor distribution in human and pig ovaries we have observed the presence of FSH (but not LH) receptors in the oocytes. FSH receptors appeared in the oocytes of primary follicles during follicular development and persisted up to the preovulatory stage. In denuded human preovulatory oocytes, FSH receptor mRNA was detected at a concentration per cell exceeding by about 20-fold that present in granulosa cells. Saturable binding of [(125)I]FSH to the membrane of oocytes was demonstrated by autoradiography. When incubated with FSH, denuded oocytes responded by a mobilization of Ca(2+). These observations concur to demonstrate the presence of functional FSH receptors in oocytes and raise the possibility of direct control of oocyte development by FSH.</description><subject>Adult</subject><subject>Animals</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Calcium - metabolism</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - metabolism</subject><subject>Follicular Atresia - metabolism</subject><subject>Hormones. Endocrine system</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Malignant tumors</subject><subject>Medical sciences</subject><subject>Oocytes - metabolism</subject><subject>Ovarian Follicle - cytology</subject><subject>Ovarian Follicle - physiology</subject><subject>Pharmacology. Drug treatments</subject><subject>Receptors, FSH - genetics</subject><subject>Receptors, FSH - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Staining and Labeling</subject><subject>Swine</subject><subject>Thyroid. Thyroid axis (diseases)</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M9LwzAYxvEgipvTm2fpRU-25s3P5iQynAoDLwreQpql2pE2M2kP---trLDTe_nwvPBF6BpwAQTww9YWpSx4QYgQJ2gOivFcgpKnaI4xgVxJ8jVDFyltMQbGOD1HMwClGJVsjsgqeN9Y7_LUN-3gTd9039lPiG3oXBaddbs-xJQ1XRaC3fcuPV6is9r45K6mu0Cfq-eP5Wu-fn95Wz6tc0tB9jmDygIVDBg1wAWtJFeOgCQ1lLyUvKKlNIoZaWlFNoqyimInnXMEl6pkki7Q3WF3F8Pv4FKv2yZZ573pXBiSliAEw4BHeH-ANoaUoqv1LjatiXsNWP830lurS6m5_m808ptpd6hatzniKcoIbidgkjW-jqazTTo6KgQfn9M_3uptDw</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>MEDURI, Geri</creator><creator>CHARNAUX, Nathalie</creator><creator>DRIANCOURT, Marc-Antoine</creator><creator>COMBETTES, Laurent</creator><creator>GRANET, Philippe</creator><creator>VANNIER, Brigitte</creator><creator>LOOSFELT, Hugues</creator><creator>MILGROM, Edwin</creator><general>Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020501</creationdate><title>Follicle-stimulating hormone receptors in oocytes?</title><author>MEDURI, Geri ; CHARNAUX, Nathalie ; DRIANCOURT, Marc-Antoine ; COMBETTES, Laurent ; GRANET, Philippe ; VANNIER, Brigitte ; LOOSFELT, Hugues ; MILGROM, Edwin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-41bc1364143a1563b759e2172f185875b387a94a7c3b2d934b30e7eee20898473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Calcium - metabolism</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - metabolism</topic><topic>Follicular Atresia - metabolism</topic><topic>Hormones. Endocrine system</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Malignant tumors</topic><topic>Medical sciences</topic><topic>Oocytes - metabolism</topic><topic>Ovarian Follicle - cytology</topic><topic>Ovarian Follicle - physiology</topic><topic>Pharmacology. Drug treatments</topic><topic>Receptors, FSH - genetics</topic><topic>Receptors, FSH - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Staining and Labeling</topic><topic>Swine</topic><topic>Thyroid. Thyroid axis (diseases)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MEDURI, Geri</creatorcontrib><creatorcontrib>CHARNAUX, Nathalie</creatorcontrib><creatorcontrib>DRIANCOURT, Marc-Antoine</creatorcontrib><creatorcontrib>COMBETTES, Laurent</creatorcontrib><creatorcontrib>GRANET, Philippe</creatorcontrib><creatorcontrib>VANNIER, Brigitte</creatorcontrib><creatorcontrib>LOOSFELT, Hugues</creatorcontrib><creatorcontrib>MILGROM, Edwin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MEDURI, Geri</au><au>CHARNAUX, Nathalie</au><au>DRIANCOURT, Marc-Antoine</au><au>COMBETTES, Laurent</au><au>GRANET, Philippe</au><au>VANNIER, Brigitte</au><au>LOOSFELT, Hugues</au><au>MILGROM, Edwin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Follicle-stimulating hormone receptors in oocytes?</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>87</volume><issue>5</issue><spage>2266</spage><epage>2276</epage><pages>2266-2276</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>The regulatory mechanisms of oocyte maturation remain poorly understood. Although gonadotropins play a major role in these processes, they have generally been considered to act on somatic supportive cells, but not directly on germ cells. We have raised high affinity monoclonal antibodies against LH and FSH receptors. When using the latter to study receptor distribution in human and pig ovaries we have observed the presence of FSH (but not LH) receptors in the oocytes. FSH receptors appeared in the oocytes of primary follicles during follicular development and persisted up to the preovulatory stage. In denuded human preovulatory oocytes, FSH receptor mRNA was detected at a concentration per cell exceeding by about 20-fold that present in granulosa cells. Saturable binding of [(125)I]FSH to the membrane of oocytes was demonstrated by autoradiography. When incubated with FSH, denuded oocytes responded by a mobilization of Ca(2+). These observations concur to demonstrate the presence of functional FSH receptors in oocytes and raise the possibility of direct control of oocyte development by FSH.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>11994374</pmid><doi>10.1210/jc.87.5.2266</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adult Animals Autoradiography Biological and medical sciences Biological Transport - drug effects Calcium - metabolism Endocrinopathies Female Follicle Stimulating Hormone - metabolism Follicular Atresia - metabolism Hormones. Endocrine system Humans Immunohistochemistry - methods Malignant tumors Medical sciences Oocytes - metabolism Ovarian Follicle - cytology Ovarian Follicle - physiology Pharmacology. Drug treatments Receptors, FSH - genetics Receptors, FSH - metabolism RNA, Messenger - metabolism Staining and Labeling Swine Thyroid. Thyroid axis (diseases) |
| title | Follicle-stimulating hormone receptors in oocytes? |
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