Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae)
Sixteen Pythium isolates from diverse hosts and locations, which showed similarities in their morphology and sequences of the internal transcribed spacer (ITS) region of their rRNA gene, were investigated. As opposed to the generally accepted view, within single isolates ITS sequence variations were...
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| Veröffentlicht in: | FEMS microbiology letters 2008-07, Vol.284 (1), p.17-27 |
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| creator | Belbahri, Lassaad McLeod, Adèle Paul, Bernard Calmin, Gautier Moralejo, Eduardo Spies, Chris F.J Botha, Wilhelm J Clemente, Antonio Descals, Enrique Sánchez-Hernández, Esperanza Lefort, Francois |
| description | Sixteen Pythium isolates from diverse hosts and locations, which showed similarities in their morphology and sequences of the internal transcribed spacer (ITS) region of their rRNA gene, were investigated. As opposed to the generally accepted view, within single isolates ITS sequence variations were consistently found mostly as part of a tract of identical bases (A-T) within ITS1, and of GT or GTTT repeats within the ITS2 sequence. Thirty-one different ITS sequences obtained from 39 cloned ITS products from the 16 isolates showed high sequence and length polymorphisms within and between isolates. However, in a phylogenetic analysis, they formed a cluster distinct from those of other Pythium species. Additional sequencing of two nuclear genes (elongation factor 1α and β-tubulin) and one mitochondrial gene (nadh1) revealed high levels of heterozygosity as well as polymorphism within and between isolates, with some isolates possessing two or more alleles for each of the nuclear genes. In contrast to the observed variation in the ITS and other gene areas, all isolates were phenotypically similar. Pythium mercuriale sp. nov. (Pythiaceae) is characterized by forming thin-walled chlamydospores, subglobose to obovoid, papillate sporangia proliferating internally and smooth-walled oogonia surrounded by multiple antheridia. Maximum likelihood phylogenetic analyses based on both ITS and β-tubulin sequence data place P. mercuriale in a clade between Pythium and Phytophthora. |
| doi_str_mv | 10.1111/j.1574-6968.2008.01168.x |
| format | Article |
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(Pythiaceae)</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Belbahri, Lassaad ; McLeod, Adèle ; Paul, Bernard ; Calmin, Gautier ; Moralejo, Eduardo ; Spies, Chris F.J ; Botha, Wilhelm J ; Clemente, Antonio ; Descals, Enrique ; Sánchez-Hernández, Esperanza ; Lefort, Francois</creator><creatorcontrib>Belbahri, Lassaad ; McLeod, Adèle ; Paul, Bernard ; Calmin, Gautier ; Moralejo, Eduardo ; Spies, Chris F.J ; Botha, Wilhelm J ; Clemente, Antonio ; Descals, Enrique ; Sánchez-Hernández, Esperanza ; Lefort, Francois</creatorcontrib><description>Sixteen Pythium isolates from diverse hosts and locations, which showed similarities in their morphology and sequences of the internal transcribed spacer (ITS) region of their rRNA gene, were investigated. As opposed to the generally accepted view, within single isolates ITS sequence variations were consistently found mostly as part of a tract of identical bases (A-T) within ITS1, and of GT or GTTT repeats within the ITS2 sequence. Thirty-one different ITS sequences obtained from 39 cloned ITS products from the 16 isolates showed high sequence and length polymorphisms within and between isolates. However, in a phylogenetic analysis, they formed a cluster distinct from those of other Pythium species. Additional sequencing of two nuclear genes (elongation factor 1α and β-tubulin) and one mitochondrial gene (nadh1) revealed high levels of heterozygosity as well as polymorphism within and between isolates, with some isolates possessing two or more alleles for each of the nuclear genes. In contrast to the observed variation in the ITS and other gene areas, all isolates were phenotypically similar. Pythium mercuriale sp. nov. (Pythiaceae) is characterized by forming thin-walled chlamydospores, subglobose to obovoid, papillate sporangia proliferating internally and smooth-walled oogonia surrounded by multiple antheridia. Maximum likelihood phylogenetic analyses based on both ITS and β-tubulin sequence data place P. mercuriale in a clade between Pythium and Phytophthora.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2008.01168.x</identifier><identifier>PMID: 18479434</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Algal Proteins - genetics ; Alleles ; Biological and medical sciences ; Chlamydospores ; Cluster Analysis ; DNA, Algal - chemistry ; DNA, Algal - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; DNA, Ribosomal Spacer - chemistry ; DNA, Ribosomal Spacer - genetics ; Elongation ; Fundamental and applied biological sciences. Psychology ; Gene polymorphism ; Gene sequencing ; Genes ; Genes, rRNA ; Genotype ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Heterozygosity ; Heterozygote ; ITS heterogeneity ; Microbiology ; Mitochondria ; molecular phylogeny ; Molecular Sequence Data ; Morphology ; Mycology ; New species ; Oogonia ; Oomycetes ; Peptide Elongation Factor 1 - genetics ; Phenotype ; Phylogenetics ; Phylogeny ; plant pathogens ; Polymorphism ; Polymorphism, Genetic ; Pythiaceae ; Pythium ; Pythium - classification ; Pythium - cytology ; Pythium - genetics ; Pythium - isolation & purification ; Pythium mercuriale ; rRNA ; Sequence Analysis, DNA ; Sporangia ; Tubulin ; Tubulin - genetics ; Variation</subject><ispartof>FEMS microbiology letters, 2008-07, Vol.284 (1), p.17-27</ispartof><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2008</rights><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5248-c6fe6d91d4fe5285f35682ac1249ef4e8854a8ef6846928e097fd598d5d9c50a3</citedby><cites>FETCH-LOGICAL-c5248-c6fe6d91d4fe5285f35682ac1249ef4e8854a8ef6846928e097fd598d5d9c50a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2008.01168.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2008.01168.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20410292$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18479434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belbahri, Lassaad</creatorcontrib><creatorcontrib>McLeod, Adèle</creatorcontrib><creatorcontrib>Paul, Bernard</creatorcontrib><creatorcontrib>Calmin, Gautier</creatorcontrib><creatorcontrib>Moralejo, Eduardo</creatorcontrib><creatorcontrib>Spies, Chris F.J</creatorcontrib><creatorcontrib>Botha, Wilhelm J</creatorcontrib><creatorcontrib>Clemente, Antonio</creatorcontrib><creatorcontrib>Descals, Enrique</creatorcontrib><creatorcontrib>Sánchez-Hernández, Esperanza</creatorcontrib><creatorcontrib>Lefort, Francois</creatorcontrib><title>Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae)</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Sixteen Pythium isolates from diverse hosts and locations, which showed similarities in their morphology and sequences of the internal transcribed spacer (ITS) region of their rRNA gene, were investigated. As opposed to the generally accepted view, within single isolates ITS sequence variations were consistently found mostly as part of a tract of identical bases (A-T) within ITS1, and of GT or GTTT repeats within the ITS2 sequence. Thirty-one different ITS sequences obtained from 39 cloned ITS products from the 16 isolates showed high sequence and length polymorphisms within and between isolates. However, in a phylogenetic analysis, they formed a cluster distinct from those of other Pythium species. Additional sequencing of two nuclear genes (elongation factor 1α and β-tubulin) and one mitochondrial gene (nadh1) revealed high levels of heterozygosity as well as polymorphism within and between isolates, with some isolates possessing two or more alleles for each of the nuclear genes. In contrast to the observed variation in the ITS and other gene areas, all isolates were phenotypically similar. Pythium mercuriale sp. nov. (Pythiaceae) is characterized by forming thin-walled chlamydospores, subglobose to obovoid, papillate sporangia proliferating internally and smooth-walled oogonia surrounded by multiple antheridia. Maximum likelihood phylogenetic analyses based on both ITS and β-tubulin sequence data place P. mercuriale in a clade between Pythium and Phytophthora.</description><subject>Algal Proteins - genetics</subject><subject>Alleles</subject><subject>Biological and medical sciences</subject><subject>Chlamydospores</subject><subject>Cluster Analysis</subject><subject>DNA, Algal - chemistry</subject><subject>DNA, Algal - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>DNA, Ribosomal Spacer - chemistry</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Elongation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene polymorphism</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, rRNA</subject><subject>Genotype</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Heterozygosity</subject><subject>Heterozygote</subject><subject>ITS heterogeneity</subject><subject>Microbiology</subject><subject>Mitochondria</subject><subject>molecular phylogeny</subject><subject>Molecular Sequence Data</subject><subject>Morphology</subject><subject>Mycology</subject><subject>New species</subject><subject>Oogonia</subject><subject>Oomycetes</subject><subject>Peptide Elongation Factor 1 - genetics</subject><subject>Phenotype</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>plant pathogens</subject><subject>Polymorphism</subject><subject>Polymorphism, Genetic</subject><subject>Pythiaceae</subject><subject>Pythium</subject><subject>Pythium - classification</subject><subject>Pythium - cytology</subject><subject>Pythium - genetics</subject><subject>Pythium - isolation & purification</subject><subject>Pythium mercuriale</subject><subject>rRNA</subject><subject>Sequence Analysis, DNA</subject><subject>Sporangia</subject><subject>Tubulin</subject><subject>Tubulin - genetics</subject><subject>Variation</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkV1rFDEUhgdR7Fr9CxoQpV7MmO_JXHhRitWF9QPbXoeYOdlmmc2syUzbpX_eTHepIIrmJgfO8745J29RIIIrks_bVUVEzUvZSFVRjFWFCcnlzYNidt94WMwwq1VJcFMfFE9SWmGMOcXycXFAFK8bzvisuJ2HIZq0Aeudt8iEFl374dKH0qe-MwOgBD9GCBbQlYneDL4PyAc0XAKan5-h-O3zMVpCABRhOfV6h75us8G4RmuIdsyaLptsKhT6qwod3TWNBQNvnhaPnOkSPNvfh8XF6fvzk4_l4suH-cnxorSCclVa6UC2DWm5A0GVcExIRY0llDfgOCgluFHgpOKyoQryvq4VjWpF21iBDTssXu98N7HPu6RBr32y0HUmQD8mXRMpGRPsn2D-aoZ5zTP48jdw1Y8x5CU0ZVgK2jCpMqV2lI19ShGc3kS_NnGrCdZTjnqlp7j0FNdkrvRdjvomS5_vHxi_r6H9JdwHl4FXe8AkazoXTbA-3XMUc4JpQzP3bsdd-w62_z2APv20mKqsZzt9P27-oi7_NP6LncqZXptlzJNdnFFMGMYNppQS9hMaG8_2</recordid><startdate>200807</startdate><enddate>200807</enddate><creator>Belbahri, Lassaad</creator><creator>McLeod, Adèle</creator><creator>Paul, Bernard</creator><creator>Calmin, Gautier</creator><creator>Moralejo, Eduardo</creator><creator>Spies, Chris F.J</creator><creator>Botha, Wilhelm J</creator><creator>Clemente, Antonio</creator><creator>Descals, Enrique</creator><creator>Sánchez-Hernández, Esperanza</creator><creator>Lefort, Francois</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200807</creationdate><title>Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae)</title><author>Belbahri, Lassaad ; McLeod, Adèle ; Paul, Bernard ; Calmin, Gautier ; Moralejo, Eduardo ; Spies, Chris F.J ; Botha, Wilhelm J ; Clemente, Antonio ; Descals, Enrique ; Sánchez-Hernández, Esperanza ; Lefort, Francois</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5248-c6fe6d91d4fe5285f35682ac1249ef4e8854a8ef6846928e097fd598d5d9c50a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Algal Proteins - genetics</topic><topic>Alleles</topic><topic>Biological and medical sciences</topic><topic>Chlamydospores</topic><topic>Cluster Analysis</topic><topic>DNA, Algal - chemistry</topic><topic>DNA, Algal - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>DNA, Ribosomal Spacer - chemistry</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Elongation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene polymorphism</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, rRNA</topic><topic>Genotype</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Heterozygosity</topic><topic>Heterozygote</topic><topic>ITS heterogeneity</topic><topic>Microbiology</topic><topic>Mitochondria</topic><topic>molecular phylogeny</topic><topic>Molecular Sequence Data</topic><topic>Morphology</topic><topic>Mycology</topic><topic>New species</topic><topic>Oogonia</topic><topic>Oomycetes</topic><topic>Peptide Elongation Factor 1 - genetics</topic><topic>Phenotype</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>plant pathogens</topic><topic>Polymorphism</topic><topic>Polymorphism, Genetic</topic><topic>Pythiaceae</topic><topic>Pythium</topic><topic>Pythium - classification</topic><topic>Pythium - cytology</topic><topic>Pythium - genetics</topic><topic>Pythium - isolation & purification</topic><topic>Pythium mercuriale</topic><topic>rRNA</topic><topic>Sequence Analysis, DNA</topic><topic>Sporangia</topic><topic>Tubulin</topic><topic>Tubulin - genetics</topic><topic>Variation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belbahri, Lassaad</creatorcontrib><creatorcontrib>McLeod, Adèle</creatorcontrib><creatorcontrib>Paul, Bernard</creatorcontrib><creatorcontrib>Calmin, Gautier</creatorcontrib><creatorcontrib>Moralejo, Eduardo</creatorcontrib><creatorcontrib>Spies, Chris F.J</creatorcontrib><creatorcontrib>Botha, Wilhelm J</creatorcontrib><creatorcontrib>Clemente, Antonio</creatorcontrib><creatorcontrib>Descals, Enrique</creatorcontrib><creatorcontrib>Sánchez-Hernández, Esperanza</creatorcontrib><creatorcontrib>Lefort, Francois</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belbahri, Lassaad</au><au>McLeod, Adèle</au><au>Paul, Bernard</au><au>Calmin, Gautier</au><au>Moralejo, Eduardo</au><au>Spies, Chris F.J</au><au>Botha, Wilhelm J</au><au>Clemente, Antonio</au><au>Descals, Enrique</au><au>Sánchez-Hernández, Esperanza</au><au>Lefort, Francois</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae)</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2008-07</date><risdate>2008</risdate><volume>284</volume><issue>1</issue><spage>17</spage><epage>27</epage><pages>17-27</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Sixteen Pythium isolates from diverse hosts and locations, which showed similarities in their morphology and sequences of the internal transcribed spacer (ITS) region of their rRNA gene, were investigated. As opposed to the generally accepted view, within single isolates ITS sequence variations were consistently found mostly as part of a tract of identical bases (A-T) within ITS1, and of GT or GTTT repeats within the ITS2 sequence. Thirty-one different ITS sequences obtained from 39 cloned ITS products from the 16 isolates showed high sequence and length polymorphisms within and between isolates. However, in a phylogenetic analysis, they formed a cluster distinct from those of other Pythium species. Additional sequencing of two nuclear genes (elongation factor 1α and β-tubulin) and one mitochondrial gene (nadh1) revealed high levels of heterozygosity as well as polymorphism within and between isolates, with some isolates possessing two or more alleles for each of the nuclear genes. In contrast to the observed variation in the ITS and other gene areas, all isolates were phenotypically similar. Pythium mercuriale sp. nov. (Pythiaceae) is characterized by forming thin-walled chlamydospores, subglobose to obovoid, papillate sporangia proliferating internally and smooth-walled oogonia surrounded by multiple antheridia. Maximum likelihood phylogenetic analyses based on both ITS and β-tubulin sequence data place P. mercuriale in a clade between Pythium and Phytophthora.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18479434</pmid><doi>10.1111/j.1574-6968.2008.01168.x</doi><tpages>11</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 2008-07, Vol.284 (1), p.17-27 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Algal Proteins - genetics Alleles Biological and medical sciences Chlamydospores Cluster Analysis DNA, Algal - chemistry DNA, Algal - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - genetics Elongation Fundamental and applied biological sciences. Psychology Gene polymorphism Gene sequencing Genes Genes, rRNA Genotype Growth, nutrition, metabolism, transports, enzymes. Molecular biology Heterozygosity Heterozygote ITS heterogeneity Microbiology Mitochondria molecular phylogeny Molecular Sequence Data Morphology Mycology New species Oogonia Oomycetes Peptide Elongation Factor 1 - genetics Phenotype Phylogenetics Phylogeny plant pathogens Polymorphism Polymorphism, Genetic Pythiaceae Pythium Pythium - classification Pythium - cytology Pythium - genetics Pythium - isolation & purification Pythium mercuriale rRNA Sequence Analysis, DNA Sporangia Tubulin Tubulin - genetics Variation |
| title | Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae) |
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