Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines
A series of well-defined oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded...
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| Veröffentlicht in: | Carbohydrate research 2002-04, Vol.337 (9), p.819-825 |
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| creator | Lefeber, Dirk J. Gutiérrez Gallego, Ricardo Grün, Christian H. Proietti, Daniela D'Ascenzi, Sandro Costantino, Paolo Kamerling, Johannis P. Vliegenthart, Johannes F.G. |
| description | A series of well-defined oligosaccharide fragments of the capsular polysaccharide of
Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [→3)-β-
d-Glc
pA-(1→4)-β-
d-Glc
p-(1→] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by
1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.
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| doi_str_mv | 10.1016/S0008-6215(02)00059-9 |
| format | Article |
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Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [→3)-β-
d-Glc
pA-(1→4)-β-
d-Glc
p-(1→] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by
1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.
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Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [→3)-β-
d-Glc
pA-(1→4)-β-
d-Glc
p-(1→] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by
1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.
Graphic</description><subject>Carbohydrate Sequence</subject><subject>Chromatography, Ion Exchange</subject><subject>Conjugate vaccines</subject><subject>HPAEC-PAD</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - isolation & purification</subject><subject>Oligosaccharides - metabolism</subject><subject>Polysaccharide degradation</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Polysaccharides, Bacterial - metabolism</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - chemistry</subject><subject>Vaccines, Conjugate - chemistry</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAURi1ERYfCTwC8QrAI-BF7klWFKh6VKrEoldhZN_b11FUSBzupNEv-eT0P6LILy7Lu-T7bh5A3nH3ijOvP14yxptKCqw9MfCwH1VbtM7LizVpWtdC_n5PVf-SUvMz5rhyZXusX5JTzttWNVCvy9zLHHuYQRxo9jX3YxAzW3kIKDjP1KQ4U6ARpDtBXYIOjt1uXYr_NMOMuM424DNFGa6Gn83ZCKum0n_-roT4mumSkYaQ2jnfLZhe9L_MwYn5FTjz0GV8f9zNy8-3rr4sf1dXP75cXX64qW0sxV-A60ba-tp3yEsACNqC5cg3aWqHsnK9LIXjnWFe-pzpet0qgFKC08h3KM_L-0Dul-GfBPJshZIt9DyPGJZs115oxWRdQHUCbYs4JvZlSGCBtDWdm597s3ZudWMOE2bs3bcm9PV6wdAO6x9RRdgHeHQAP0cAmhWxurkUpZGXxmrNCnB8ILCLuAyaTbcDRogsJ7WxcDE884gFl8aD9</recordid><startdate>20020430</startdate><enddate>20020430</enddate><creator>Lefeber, Dirk J.</creator><creator>Gutiérrez Gallego, Ricardo</creator><creator>Grün, Christian H.</creator><creator>Proietti, Daniela</creator><creator>D'Ascenzi, Sandro</creator><creator>Costantino, Paolo</creator><creator>Kamerling, Johannis P.</creator><creator>Vliegenthart, Johannes F.G.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020430</creationdate><title>Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines</title><author>Lefeber, Dirk J. ; Gutiérrez Gallego, Ricardo ; Grün, Christian H. ; Proietti, Daniela ; D'Ascenzi, Sandro ; Costantino, Paolo ; Kamerling, Johannis P. ; Vliegenthart, Johannes F.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-adb299f4cb5f3aacae8a615d8ec45e3bdf4accafdd0b1195b14952e32a565fbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Carbohydrate Sequence</topic><topic>Chromatography, Ion Exchange</topic><topic>Conjugate vaccines</topic><topic>HPAEC-PAD</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Sequence Data</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - isolation & purification</topic><topic>Oligosaccharides - metabolism</topic><topic>Polysaccharide degradation</topic><topic>Polysaccharides, Bacterial - chemistry</topic><topic>Polysaccharides, Bacterial - metabolism</topic><topic>Streptococcus pneumoniae</topic><topic>Streptococcus pneumoniae - chemistry</topic><topic>Vaccines, Conjugate - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lefeber, Dirk J.</creatorcontrib><creatorcontrib>Gutiérrez Gallego, Ricardo</creatorcontrib><creatorcontrib>Grün, Christian H.</creatorcontrib><creatorcontrib>Proietti, Daniela</creatorcontrib><creatorcontrib>D'Ascenzi, Sandro</creatorcontrib><creatorcontrib>Costantino, Paolo</creatorcontrib><creatorcontrib>Kamerling, Johannis P.</creatorcontrib><creatorcontrib>Vliegenthart, Johannes F.G.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lefeber, Dirk J.</au><au>Gutiérrez Gallego, Ricardo</au><au>Grün, Christian H.</au><au>Proietti, Daniela</au><au>D'Ascenzi, Sandro</au><au>Costantino, Paolo</au><au>Kamerling, Johannis P.</au><au>Vliegenthart, Johannes F.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>2002-04-30</date><risdate>2002</risdate><volume>337</volume><issue>9</issue><spage>819</spage><epage>825</epage><pages>819-825</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>A series of well-defined oligosaccharide fragments of the capsular polysaccharide of
Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [→3)-β-
d-Glc
pA-(1→4)-β-
d-Glc
p-(1→] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by
1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.
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| subjects | Carbohydrate Sequence Chromatography, Ion Exchange Conjugate vaccines HPAEC-PAD Hydrogen-Ion Concentration Hydrolysis Magnetic Resonance Spectroscopy Molecular Sequence Data Oligosaccharides - chemistry Oligosaccharides - isolation & purification Oligosaccharides - metabolism Polysaccharide degradation Polysaccharides, Bacterial - chemistry Polysaccharides, Bacterial - metabolism Streptococcus pneumoniae Streptococcus pneumoniae - chemistry Vaccines, Conjugate - chemistry |
| title | Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines |
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