Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation
Local physical interactions between cells and extracellular matrix (ECM) influence directional cell motility that is critical for tissue development, wound repair, and cancer metastasis. Here we test the possibility that the precise spatial positioning of focal adhesions governs the direction in whi...
Gespeichert in:
| Veröffentlicht in: | The FASEB journal 2008-06, Vol.22 (6), p.1649-1659 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1659 |
|---|---|
| container_issue | 6 |
| container_start_page | 1649 |
| container_title | The FASEB journal |
| container_volume | 22 |
| creator | Xia, Nan Thodeti, Charles K. Hunt, Tom P. Xu, Qiaobing Ho, Madelyn Whitesides, George M. Westervelt, Robert Ingber, Donald E. |
| description | Local physical interactions between cells and extracellular matrix (ECM) influence directional cell motility that is critical for tissue development, wound repair, and cancer metastasis. Here we test the possibility that the precise spatial positioning of focal adhesions governs the direction in which cells spread and move. NIH 3T3 cells were cultured on circular or linear ECM islands, which were created using a microcontact printing technique and were 1 μm wide and of various lengths (1 to 8 μm) and separated by 1 to 4.5 μm wide nonadhesive barrier regions. Cells could be driven proactively to spread and move in particular directions by altering either the interisland spacing or the shape of similar‐sized ECM islands. Immunofluorescence microscopy confirmed that focal adhesions assembled preferentially above the ECM islands, with the greatest staining intensity being observed at adhesion sites along the cell periphery. Rac‐FRET analysis of living cells revealed that Rac became activated within 2 min after peripheral membrane extensions adhered to new ECM islands, and this activation wave propagated outward in an oriented manner as the cells spread from island to island. A computational model, which incorporates that cells preferentially protrude membrane processes from regions near newly formed focal adhesion contacts, could predict with high accuracy the effects of six different arrangements of micropatterned ECM islands on directional cell spreading. Taken together, these results suggest that physical properties of the ECM may influence directional cell movement by dictating where cells will form new focal adhesions and activate Rac and, hence, govern where new membrane protrusions will form.— Xia N., Thodeti, C. K., Hunt, T. P., Xu, Q., Ho, M., Whitesides, G. M., Westervelt, R., Ingber D. E. Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation. FASEB J. 22, 1649–1659 (2008) |
| doi_str_mv | 10.1096/fj.07-090571 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71651197</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71651197</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4351-f581da64113d3eac8179d718fe39242af84db8a7f6694a1d6c47a8276f217a013</originalsourceid><addsrcrecordid>eNp90MFP2zAUx3ELDa0FduM8-bQTKe_Fie0cB6ywCQkJtrP1cGzqKolLnID63y9VK6FddvLl876Sf4ydIywQKnnp1wtQGVRQKjxicywFZFJL-MTmoKs8k1LoGTtJaQ0ACCg_sxlq1CBEMWftTeidHULsqOE2dkMfGx49t65peBuH0IRhy4dVH8eXFffRTozqlUvTBd_EFHanoXvh1NU8bWgI_3YeyXKa-m-0g2fs2FOT3JfDe8r-LH_8vr7L7h9uf15_v89sIUrMfKmxJlkgilo4shpVVSvU3okqL3LyuqifNSkvZVUQ1tIWinSupM9REaA4Zd_23U0fX0eXBtOGtPsSdS6OySiUJWKlJnixh7aPKfXOm00fWuq3BsHs5jV-bUCZ_bwT_3rojs-tqz_wYc8J6D14D43b_jdmlk9X-fIXqEP7Lwhuh4w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71651197</pqid></control><display><type>article</type><title>Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><creator>Xia, Nan ; Thodeti, Charles K. ; Hunt, Tom P. ; Xu, Qiaobing ; Ho, Madelyn ; Whitesides, George M. ; Westervelt, Robert ; Ingber, Donald E.</creator><creatorcontrib>Xia, Nan ; Thodeti, Charles K. ; Hunt, Tom P. ; Xu, Qiaobing ; Ho, Madelyn ; Whitesides, George M. ; Westervelt, Robert ; Ingber, Donald E.</creatorcontrib><description>Local physical interactions between cells and extracellular matrix (ECM) influence directional cell motility that is critical for tissue development, wound repair, and cancer metastasis. Here we test the possibility that the precise spatial positioning of focal adhesions governs the direction in which cells spread and move. NIH 3T3 cells were cultured on circular or linear ECM islands, which were created using a microcontact printing technique and were 1 μm wide and of various lengths (1 to 8 μm) and separated by 1 to 4.5 μm wide nonadhesive barrier regions. Cells could be driven proactively to spread and move in particular directions by altering either the interisland spacing or the shape of similar‐sized ECM islands. Immunofluorescence microscopy confirmed that focal adhesions assembled preferentially above the ECM islands, with the greatest staining intensity being observed at adhesion sites along the cell periphery. Rac‐FRET analysis of living cells revealed that Rac became activated within 2 min after peripheral membrane extensions adhered to new ECM islands, and this activation wave propagated outward in an oriented manner as the cells spread from island to island. A computational model, which incorporates that cells preferentially protrude membrane processes from regions near newly formed focal adhesion contacts, could predict with high accuracy the effects of six different arrangements of micropatterned ECM islands on directional cell spreading. Taken together, these results suggest that physical properties of the ECM may influence directional cell movement by dictating where cells will form new focal adhesions and activate Rac and, hence, govern where new membrane protrusions will form.— Xia N., Thodeti, C. K., Hunt, T. P., Xu, Q., Ho, M., Whitesides, G. M., Westervelt, R., Ingber D. E. Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation. FASEB J. 22, 1649–1659 (2008)</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.07-090571</identifier><identifier>PMID: 18180334</identifier><language>eng</language><publisher>United States: Federation of American Societies for Experimental Biology</publisher><subject>Animals ; Cell Adhesion ; Cell Movement ; Cell Surface Extensions - metabolism ; cytoskeleton ; extracellular matrix ; Extracellular Matrix - metabolism ; Extracellular Matrix - ultrastructure ; Fluorescence Resonance Energy Transfer ; Focal Adhesions ; mechanical ; Mice ; microcontact printing ; migration ; NIH 3T3 Cells ; rac GTP-Binding Proteins - metabolism ; traction</subject><ispartof>The FASEB journal, 2008-06, Vol.22 (6), p.1649-1659</ispartof><rights>FASEB</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4351-f581da64113d3eac8179d718fe39242af84db8a7f6694a1d6c47a8276f217a013</citedby><cites>FETCH-LOGICAL-c4351-f581da64113d3eac8179d718fe39242af84db8a7f6694a1d6c47a8276f217a013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.07-090571$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.07-090571$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18180334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xia, Nan</creatorcontrib><creatorcontrib>Thodeti, Charles K.</creatorcontrib><creatorcontrib>Hunt, Tom P.</creatorcontrib><creatorcontrib>Xu, Qiaobing</creatorcontrib><creatorcontrib>Ho, Madelyn</creatorcontrib><creatorcontrib>Whitesides, George M.</creatorcontrib><creatorcontrib>Westervelt, Robert</creatorcontrib><creatorcontrib>Ingber, Donald E.</creatorcontrib><title>Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>Local physical interactions between cells and extracellular matrix (ECM) influence directional cell motility that is critical for tissue development, wound repair, and cancer metastasis. Here we test the possibility that the precise spatial positioning of focal adhesions governs the direction in which cells spread and move. NIH 3T3 cells were cultured on circular or linear ECM islands, which were created using a microcontact printing technique and were 1 μm wide and of various lengths (1 to 8 μm) and separated by 1 to 4.5 μm wide nonadhesive barrier regions. Cells could be driven proactively to spread and move in particular directions by altering either the interisland spacing or the shape of similar‐sized ECM islands. Immunofluorescence microscopy confirmed that focal adhesions assembled preferentially above the ECM islands, with the greatest staining intensity being observed at adhesion sites along the cell periphery. Rac‐FRET analysis of living cells revealed that Rac became activated within 2 min after peripheral membrane extensions adhered to new ECM islands, and this activation wave propagated outward in an oriented manner as the cells spread from island to island. A computational model, which incorporates that cells preferentially protrude membrane processes from regions near newly formed focal adhesion contacts, could predict with high accuracy the effects of six different arrangements of micropatterned ECM islands on directional cell spreading. Taken together, these results suggest that physical properties of the ECM may influence directional cell movement by dictating where cells will form new focal adhesions and activate Rac and, hence, govern where new membrane protrusions will form.— Xia N., Thodeti, C. K., Hunt, T. P., Xu, Q., Ho, M., Whitesides, G. M., Westervelt, R., Ingber D. E. Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation. FASEB J. 22, 1649–1659 (2008)</description><subject>Animals</subject><subject>Cell Adhesion</subject><subject>Cell Movement</subject><subject>Cell Surface Extensions - metabolism</subject><subject>cytoskeleton</subject><subject>extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extracellular Matrix - ultrastructure</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>Focal Adhesions</subject><subject>mechanical</subject><subject>Mice</subject><subject>microcontact printing</subject><subject>migration</subject><subject>NIH 3T3 Cells</subject><subject>rac GTP-Binding Proteins - metabolism</subject><subject>traction</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90MFP2zAUx3ELDa0FduM8-bQTKe_Fie0cB6ywCQkJtrP1cGzqKolLnID63y9VK6FddvLl876Sf4ydIywQKnnp1wtQGVRQKjxicywFZFJL-MTmoKs8k1LoGTtJaQ0ACCg_sxlq1CBEMWftTeidHULsqOE2dkMfGx49t65peBuH0IRhy4dVH8eXFffRTozqlUvTBd_EFHanoXvh1NU8bWgI_3YeyXKa-m-0g2fs2FOT3JfDe8r-LH_8vr7L7h9uf15_v89sIUrMfKmxJlkgilo4shpVVSvU3okqL3LyuqifNSkvZVUQ1tIWinSupM9REaA4Zd_23U0fX0eXBtOGtPsSdS6OySiUJWKlJnixh7aPKfXOm00fWuq3BsHs5jV-bUCZ_bwT_3rojs-tqz_wYc8J6D14D43b_jdmlk9X-fIXqEP7Lwhuh4w</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Xia, Nan</creator><creator>Thodeti, Charles K.</creator><creator>Hunt, Tom P.</creator><creator>Xu, Qiaobing</creator><creator>Ho, Madelyn</creator><creator>Whitesides, George M.</creator><creator>Westervelt, Robert</creator><creator>Ingber, Donald E.</creator><general>Federation of American Societies for Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation</title><author>Xia, Nan ; Thodeti, Charles K. ; Hunt, Tom P. ; Xu, Qiaobing ; Ho, Madelyn ; Whitesides, George M. ; Westervelt, Robert ; Ingber, Donald E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4351-f581da64113d3eac8179d718fe39242af84db8a7f6694a1d6c47a8276f217a013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Cell Adhesion</topic><topic>Cell Movement</topic><topic>Cell Surface Extensions - metabolism</topic><topic>cytoskeleton</topic><topic>extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Extracellular Matrix - ultrastructure</topic><topic>Fluorescence Resonance Energy Transfer</topic><topic>Focal Adhesions</topic><topic>mechanical</topic><topic>Mice</topic><topic>microcontact printing</topic><topic>migration</topic><topic>NIH 3T3 Cells</topic><topic>rac GTP-Binding Proteins - metabolism</topic><topic>traction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Nan</creatorcontrib><creatorcontrib>Thodeti, Charles K.</creatorcontrib><creatorcontrib>Hunt, Tom P.</creatorcontrib><creatorcontrib>Xu, Qiaobing</creatorcontrib><creatorcontrib>Ho, Madelyn</creatorcontrib><creatorcontrib>Whitesides, George M.</creatorcontrib><creatorcontrib>Westervelt, Robert</creatorcontrib><creatorcontrib>Ingber, Donald E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, Nan</au><au>Thodeti, Charles K.</au><au>Hunt, Tom P.</au><au>Xu, Qiaobing</au><au>Ho, Madelyn</au><au>Whitesides, George M.</au><au>Westervelt, Robert</au><au>Ingber, Donald E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2008-06</date><risdate>2008</risdate><volume>22</volume><issue>6</issue><spage>1649</spage><epage>1659</epage><pages>1649-1659</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>Local physical interactions between cells and extracellular matrix (ECM) influence directional cell motility that is critical for tissue development, wound repair, and cancer metastasis. Here we test the possibility that the precise spatial positioning of focal adhesions governs the direction in which cells spread and move. NIH 3T3 cells were cultured on circular or linear ECM islands, which were created using a microcontact printing technique and were 1 μm wide and of various lengths (1 to 8 μm) and separated by 1 to 4.5 μm wide nonadhesive barrier regions. Cells could be driven proactively to spread and move in particular directions by altering either the interisland spacing or the shape of similar‐sized ECM islands. Immunofluorescence microscopy confirmed that focal adhesions assembled preferentially above the ECM islands, with the greatest staining intensity being observed at adhesion sites along the cell periphery. Rac‐FRET analysis of living cells revealed that Rac became activated within 2 min after peripheral membrane extensions adhered to new ECM islands, and this activation wave propagated outward in an oriented manner as the cells spread from island to island. A computational model, which incorporates that cells preferentially protrude membrane processes from regions near newly formed focal adhesion contacts, could predict with high accuracy the effects of six different arrangements of micropatterned ECM islands on directional cell spreading. Taken together, these results suggest that physical properties of the ECM may influence directional cell movement by dictating where cells will form new focal adhesions and activate Rac and, hence, govern where new membrane protrusions will form.— Xia N., Thodeti, C. K., Hunt, T. P., Xu, Q., Ho, M., Whitesides, G. M., Westervelt, R., Ingber D. E. Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation. FASEB J. 22, 1649–1659 (2008)</abstract><cop>United States</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>18180334</pmid><doi>10.1096/fj.07-090571</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0892-6638 |
| ispartof | The FASEB journal, 2008-06, Vol.22 (6), p.1649-1659 |
| issn | 0892-6638 1530-6860 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71651197 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Animals Cell Adhesion Cell Movement Cell Surface Extensions - metabolism cytoskeleton extracellular matrix Extracellular Matrix - metabolism Extracellular Matrix - ultrastructure Fluorescence Resonance Energy Transfer Focal Adhesions mechanical Mice microcontact printing migration NIH 3T3 Cells rac GTP-Binding Proteins - metabolism traction |
| title | Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T15%3A25%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Directional%20control%20of%20cell%20motility%20through%20focal%20adhesion%20positioning%20and%20spatial%20control%20of%20Rac%20activation&rft.jtitle=The%20FASEB%20journal&rft.au=Xia,%20Nan&rft.date=2008-06&rft.volume=22&rft.issue=6&rft.spage=1649&rft.epage=1659&rft.pages=1649-1659&rft.issn=0892-6638&rft.eissn=1530-6860&rft_id=info:doi/10.1096/fj.07-090571&rft_dat=%3Cproquest_cross%3E71651197%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71651197&rft_id=info:pmid/18180334&rfr_iscdi=true |