Effect of Baicalein on Apoptosis of the Human Hep G2 Cell Line was Induced by Mitochondrial Dysfunction
Abstract The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time-dependent manner. The apoptotic features such as chromatin condensation and DNA fra...
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| Veröffentlicht in: | Planta medica 2002-04, Vol.68 (4), p.302-306 |
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| creator | Chang, Wen-Huei Chen, Ching-Hsein Gau, Rung-Jiun Lin, Chun-Ching Tsai, Ching-Lin Tsai, Kelvin Lu, Fung-Jou |
| description | Abstract
The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time-dependent manner. The apoptotic features such as chromatin condensation and DNA fragmentation were observed in the baicalein-treated cells. During the process of apoptosis, we noticed a sequential dissipation of mitochondrial membrane potential (ΔΨ
m
) and an apparent redistribution of cytochrome c from the mitochondria to the cytosol in baicalein-treated cells. Furthermore, the mitochondrial Bcl-2 protein represented a dramatic change in response to baicalein treatment. Altogether, our data suggested that the effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction and Bcl-2 regulation. |
| doi_str_mv | 10.1055/s-2002-26760 |
| format | Article |
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The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time-dependent manner. The apoptotic features such as chromatin condensation and DNA fragmentation were observed in the baicalein-treated cells. During the process of apoptosis, we noticed a sequential dissipation of mitochondrial membrane potential (ΔΨ
m
) and an apparent redistribution of cytochrome c from the mitochondria to the cytosol in baicalein-treated cells. Furthermore, the mitochondrial Bcl-2 protein represented a dramatic change in response to baicalein treatment. Altogether, our data suggested that the effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction and Bcl-2 regulation.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2002-26760</identifier><identifier>PMID: 11988851</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Antineoplastic agents ; Apoptosis - drug effects ; Biological and medical sciences ; Blotting, Western ; Carcinoma, Hepatocellular - drug therapy ; Cell Survival - drug effects ; Chemotherapy ; Chromatin - drug effects ; Chromatin - metabolism ; Cytochrome c Group - drug effects ; Cytochrome c Group - metabolism ; DNA - drug effects ; DNA - metabolism ; Drugs, Chinese Herbal ; Flavanones ; Flavonoids - pharmacology ; General pharmacology ; Humans ; Lamiaceae ; Medical sciences ; Membrane Potentials - drug effects ; Mitochondria - drug effects ; Mitochondria - physiology ; Original Paper ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - pharmacology ; Plant Roots - chemistry ; Proto-Oncogene Proteins c-bcl-2 - drug effects ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Tumor Cells, Cultured</subject><ispartof>Planta medica, 2002-04, Vol.68 (4), p.302-306</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3340-635ff3d33f30f6679344a3220fb5f47aff8eba1c6479e0e29fda40b46c1659083</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2002-26760.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2002-26760$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3004,3005,27901,27902,54534,54535</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13647953$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11988851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Wen-Huei</creatorcontrib><creatorcontrib>Chen, Ching-Hsein</creatorcontrib><creatorcontrib>Gau, Rung-Jiun</creatorcontrib><creatorcontrib>Lin, Chun-Ching</creatorcontrib><creatorcontrib>Tsai, Ching-Lin</creatorcontrib><creatorcontrib>Tsai, Kelvin</creatorcontrib><creatorcontrib>Lu, Fung-Jou</creatorcontrib><title>Effect of Baicalein on Apoptosis of the Human Hep G2 Cell Line was Induced by Mitochondrial Dysfunction</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time-dependent manner. The apoptotic features such as chromatin condensation and DNA fragmentation were observed in the baicalein-treated cells. During the process of apoptosis, we noticed a sequential dissipation of mitochondrial membrane potential (ΔΨ
m
) and an apparent redistribution of cytochrome c from the mitochondria to the cytosol in baicalein-treated cells. Furthermore, the mitochondrial Bcl-2 protein represented a dramatic change in response to baicalein treatment. Altogether, our data suggested that the effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction and Bcl-2 regulation.</description><subject>Antineoplastic agents</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Carcinoma, Hepatocellular - drug therapy</subject><subject>Cell Survival - drug effects</subject><subject>Chemotherapy</subject><subject>Chromatin - drug effects</subject><subject>Chromatin - metabolism</subject><subject>Cytochrome c Group - drug effects</subject><subject>Cytochrome c Group - metabolism</subject><subject>DNA - drug effects</subject><subject>DNA - metabolism</subject><subject>Drugs, Chinese Herbal</subject><subject>Flavanones</subject><subject>Flavonoids - pharmacology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Lamiaceae</subject><subject>Medical sciences</subject><subject>Membrane Potentials - drug effects</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - physiology</subject><subject>Original Paper</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Roots - chemistry</subject><subject>Proto-Oncogene Proteins c-bcl-2 - drug effects</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Tumor Cells, Cultured</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0L9v1DAUwHGrArVHy8aMvNAFQp9_xInH9ii9Soe6lNlynGfOVWKHOBG6_54cd1IXJg_-6L2nLyEfGHxlUJY3ueAAvOCqUnBGVkwKXQDn7A1ZAQhegJbigrzL-QWASQ1wTi4Y03Vdl2xFft17j26iydM7G5ztMESaIr0d0jClHPLhZ9oh3cy9jXSDA33gdI1dR7chIv1jM32M7eywpc2e_ghTcrsU2zHYjn7bZz9HN4UUr8hbb7uM70_vJfn5_f55vSm2Tw-P69tt4YSQUChRei9aIbwAr1SlhZRWcA6-Kb2srPc1NpY5JSuNgFz71kpopHJMlRpqcUmuj3OHMf2eMU-mD9kt59qIac6mYkpqXsECvxyhG1POI3ozjKG3494wMIewJptDWPMv7MI_nubOTY_tKz6VXMCnE7B5yehHG13Ir04cTi7F4j4f3bQL2KN5SfMYlyL_X_sX8mKMLA</recordid><startdate>200204</startdate><enddate>200204</enddate><creator>Chang, Wen-Huei</creator><creator>Chen, Ching-Hsein</creator><creator>Gau, Rung-Jiun</creator><creator>Lin, Chun-Ching</creator><creator>Tsai, Ching-Lin</creator><creator>Tsai, Kelvin</creator><creator>Lu, Fung-Jou</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200204</creationdate><title>Effect of Baicalein on Apoptosis of the Human Hep G2 Cell Line was Induced by Mitochondrial Dysfunction</title><author>Chang, Wen-Huei ; Chen, Ching-Hsein ; Gau, Rung-Jiun ; Lin, Chun-Ching ; Tsai, Ching-Lin ; Tsai, Kelvin ; Lu, Fung-Jou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3340-635ff3d33f30f6679344a3220fb5f47aff8eba1c6479e0e29fda40b46c1659083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Antineoplastic agents</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Carcinoma, Hepatocellular - drug therapy</topic><topic>Cell Survival - drug effects</topic><topic>Chemotherapy</topic><topic>Chromatin - drug effects</topic><topic>Chromatin - metabolism</topic><topic>Cytochrome c Group - drug effects</topic><topic>Cytochrome c Group - metabolism</topic><topic>DNA - drug effects</topic><topic>DNA - metabolism</topic><topic>Drugs, Chinese Herbal</topic><topic>Flavanones</topic><topic>Flavonoids - pharmacology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Lamiaceae</topic><topic>Medical sciences</topic><topic>Membrane Potentials - drug effects</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - physiology</topic><topic>Original Paper</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Roots - chemistry</topic><topic>Proto-Oncogene Proteins c-bcl-2 - drug effects</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Wen-Huei</creatorcontrib><creatorcontrib>Chen, Ching-Hsein</creatorcontrib><creatorcontrib>Gau, Rung-Jiun</creatorcontrib><creatorcontrib>Lin, Chun-Ching</creatorcontrib><creatorcontrib>Tsai, Ching-Lin</creatorcontrib><creatorcontrib>Tsai, Kelvin</creatorcontrib><creatorcontrib>Lu, Fung-Jou</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Wen-Huei</au><au>Chen, Ching-Hsein</au><au>Gau, Rung-Jiun</au><au>Lin, Chun-Ching</au><au>Tsai, Ching-Lin</au><au>Tsai, Kelvin</au><au>Lu, Fung-Jou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Baicalein on Apoptosis of the Human Hep G2 Cell Line was Induced by Mitochondrial Dysfunction</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2002-04</date><risdate>2002</risdate><volume>68</volume><issue>4</issue><spage>302</spage><epage>306</epage><pages>302-306</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time-dependent manner. The apoptotic features such as chromatin condensation and DNA fragmentation were observed in the baicalein-treated cells. During the process of apoptosis, we noticed a sequential dissipation of mitochondrial membrane potential (ΔΨ
m
) and an apparent redistribution of cytochrome c from the mitochondria to the cytosol in baicalein-treated cells. Furthermore, the mitochondrial Bcl-2 protein represented a dramatic change in response to baicalein treatment. Altogether, our data suggested that the effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction and Bcl-2 regulation.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>11988851</pmid><doi>10.1055/s-2002-26760</doi><tpages>5</tpages></addata></record> |
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| subjects | Antineoplastic agents Apoptosis - drug effects Biological and medical sciences Blotting, Western Carcinoma, Hepatocellular - drug therapy Cell Survival - drug effects Chemotherapy Chromatin - drug effects Chromatin - metabolism Cytochrome c Group - drug effects Cytochrome c Group - metabolism DNA - drug effects DNA - metabolism Drugs, Chinese Herbal Flavanones Flavonoids - pharmacology General pharmacology Humans Lamiaceae Medical sciences Membrane Potentials - drug effects Mitochondria - drug effects Mitochondria - physiology Original Paper Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - pharmacology Plant Roots - chemistry Proto-Oncogene Proteins c-bcl-2 - drug effects Proto-Oncogene Proteins c-bcl-2 - metabolism Tumor Cells, Cultured |
| title | Effect of Baicalein on Apoptosis of the Human Hep G2 Cell Line was Induced by Mitochondrial Dysfunction |
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