mRNA expression of T-helper 1, T-helper 2 cytokines in autoimmune hepatitis in childhood
Background: In the pathology of autoimmune hepatitis the immunity mechanism of T‐helper 1 (Th1) and Th2 cells was recently evaluated. The purpose of the present study was to measure the mRNA levels in peripheral mononuclear cells and serum cytokines obtained from children with autoimmune hepatitis f...
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| Veröffentlicht in: | Pediatrics international 2008-06, Vol.50 (3), p.284-286 |
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| creator | Kawashima, Hisashi Kato, Naoki Ioi, Hiroaki Nishimata, Shigeo Watanabe, Chiako Kashiwagi, Yasuyo Takekuma, Kouji Hoshika, Akinori Szenborn, Leszek Bergman, Kacprzak |
| description | Background: In the pathology of autoimmune hepatitis the immunity mechanism of T‐helper 1 (Th1) and Th2 cells was recently evaluated. The purpose of the present study was to measure the mRNA levels in peripheral mononuclear cells and serum cytokines obtained from children with autoimmune hepatitis for a better understanding of the mechanism.
Methods: Twenty‐five patients with autoimmune hepatitis and seven controls were enrolled. mRNA levels in peripheral mononuclear cells and serum cytokines were measured using real‐time polymerase chain reaction and immunoassay.
Results: Serum interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) were rarely detected. In contrast the IFN‐γ/β‐actin mRNA levels were high.
Conclusion: Autoimmune hepatitis is a Th1‐predominant state, therefore immune modulation therapies that target the control of Th1 cytokines should be used. |
| doi_str_mv | 10.1111/j.1442-200X.2008.02584.x |
| format | Article |
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Methods: Twenty‐five patients with autoimmune hepatitis and seven controls were enrolled. mRNA levels in peripheral mononuclear cells and serum cytokines were measured using real‐time polymerase chain reaction and immunoassay.
Results: Serum interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) were rarely detected. In contrast the IFN‐γ/β‐actin mRNA levels were high.
Conclusion: Autoimmune hepatitis is a Th1‐predominant state, therefore immune modulation therapies that target the control of Th1 cytokines should be used.</description><identifier>ISSN: 1328-8067</identifier><identifier>EISSN: 1442-200X</identifier><identifier>DOI: 10.1111/j.1442-200X.2008.02584.x</identifier><identifier>PMID: 18533937</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Actins - biosynthesis ; Actins - genetics ; Adolescent ; Adult ; Autoimmune diseases ; autoimmune hepatitis ; Biomarkers - blood ; Child ; Child, Preschool ; childhood ; Cytokines ; Gene Expression ; Hepatitis ; Hepatitis, Autoimmune - blood ; Hepatitis, Autoimmune - genetics ; Hepatitis, Autoimmune - immunology ; Humans ; Immune system ; Immunoassay ; Interferon-gamma - biosynthesis ; Interferon-gamma - genetics ; Interleukin-4 - biosynthesis ; Interleukin-4 - genetics ; Leukocytes ; mRNA ; Pediatrics ; Polymerase Chain Reaction ; Prognosis ; RNA, Messenger - genetics ; Th1 ; Th1 Cells - immunology ; Th1 Cells - metabolism ; Th2 ; Th2 Cells - immunology ; Th2 Cells - metabolism</subject><ispartof>Pediatrics international, 2008-06, Vol.50 (3), p.284-286</ispartof><rights>2008 Japan Pediatric Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4874-d50a4354ea4724c5f54b4928f7a51891cb6febbd3a2b96a2e11f076977df4b53</citedby><cites>FETCH-LOGICAL-c4874-d50a4354ea4724c5f54b4928f7a51891cb6febbd3a2b96a2e11f076977df4b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1442-200X.2008.02584.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1442-200X.2008.02584.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18533937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawashima, Hisashi</creatorcontrib><creatorcontrib>Kato, Naoki</creatorcontrib><creatorcontrib>Ioi, Hiroaki</creatorcontrib><creatorcontrib>Nishimata, Shigeo</creatorcontrib><creatorcontrib>Watanabe, Chiako</creatorcontrib><creatorcontrib>Kashiwagi, Yasuyo</creatorcontrib><creatorcontrib>Takekuma, Kouji</creatorcontrib><creatorcontrib>Hoshika, Akinori</creatorcontrib><creatorcontrib>Szenborn, Leszek</creatorcontrib><creatorcontrib>Bergman, Kacprzak</creatorcontrib><title>mRNA expression of T-helper 1, T-helper 2 cytokines in autoimmune hepatitis in childhood</title><title>Pediatrics international</title><addtitle>Pediatr Int</addtitle><description>Background: In the pathology of autoimmune hepatitis the immunity mechanism of T‐helper 1 (Th1) and Th2 cells was recently evaluated. The purpose of the present study was to measure the mRNA levels in peripheral mononuclear cells and serum cytokines obtained from children with autoimmune hepatitis for a better understanding of the mechanism.
Methods: Twenty‐five patients with autoimmune hepatitis and seven controls were enrolled. mRNA levels in peripheral mononuclear cells and serum cytokines were measured using real‐time polymerase chain reaction and immunoassay.
Results: Serum interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) were rarely detected. In contrast the IFN‐γ/β‐actin mRNA levels were high.
Conclusion: Autoimmune hepatitis is a Th1‐predominant state, therefore immune modulation therapies that target the control of Th1 cytokines should be used.</description><subject>Actins - biosynthesis</subject><subject>Actins - genetics</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Autoimmune diseases</subject><subject>autoimmune hepatitis</subject><subject>Biomarkers - blood</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>childhood</subject><subject>Cytokines</subject><subject>Gene Expression</subject><subject>Hepatitis</subject><subject>Hepatitis, Autoimmune - blood</subject><subject>Hepatitis, Autoimmune - genetics</subject><subject>Hepatitis, Autoimmune - immunology</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunoassay</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - genetics</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Interleukin-4 - genetics</subject><subject>Leukocytes</subject><subject>mRNA</subject><subject>Pediatrics</subject><subject>Polymerase Chain Reaction</subject><subject>Prognosis</subject><subject>RNA, Messenger - genetics</subject><subject>Th1</subject><subject>Th1 Cells - immunology</subject><subject>Th1 Cells - metabolism</subject><subject>Th2</subject><subject>Th2 Cells - immunology</subject><subject>Th2 Cells - metabolism</subject><issn>1328-8067</issn><issn>1442-200X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS0EoqXwF1DEghUJfsb2gkVVSotUDQ-NRHeWk9xoPE3i1E7EzL_H6YxaiQ14cX1kf-dIVwehjOCCpPNxWxDOaU4xvi3SUAWmQvFi9wydPn48T5pRlStcyhP0KsYtTqRU_CU6IUowppk8Rbf9z9V5BrsxQIzOD5lvs3W-gW6EkJEPT5pm9X7yd26AmLkhs_PkXd_PA2QbGO3kJvfwXm9c12y8b16jF63tIrw53mdo_eVyfXGd33y7-npxfpPXXEmeNwJbzgQHyyXltWgFr7imqpVWEKVJXZUtVFXDLK10aSkQ0mJZaimblleCnaH3h9gx-PsZ4mR6F2voOjuAn6ORpOSSa_xPkOiEMkUS-O4vcOvnMKQdDCVUJI7yBKkDVAcfY4DWjMH1NuwNwWapyGzN0oRZmliGMg8VmV2yvj3mz1UPzZPx2EkCPh2A366D_X8Hm--XnxeV_PnB7-IEu0e_DXemlEwK82t1ZTQVml_zlfnB_gCgq6zw</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Kawashima, Hisashi</creator><creator>Kato, Naoki</creator><creator>Ioi, Hiroaki</creator><creator>Nishimata, Shigeo</creator><creator>Watanabe, Chiako</creator><creator>Kashiwagi, Yasuyo</creator><creator>Takekuma, Kouji</creator><creator>Hoshika, Akinori</creator><creator>Szenborn, Leszek</creator><creator>Bergman, Kacprzak</creator><general>Blackwell Publishing Asia</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7T5</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>mRNA expression of T-helper 1, T-helper 2 cytokines in autoimmune hepatitis in childhood</title><author>Kawashima, Hisashi ; Kato, Naoki ; Ioi, Hiroaki ; Nishimata, Shigeo ; Watanabe, Chiako ; Kashiwagi, Yasuyo ; Takekuma, Kouji ; Hoshika, Akinori ; Szenborn, Leszek ; Bergman, Kacprzak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4874-d50a4354ea4724c5f54b4928f7a51891cb6febbd3a2b96a2e11f076977df4b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Actins - biosynthesis</topic><topic>Actins - genetics</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Autoimmune diseases</topic><topic>autoimmune hepatitis</topic><topic>Biomarkers - blood</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>childhood</topic><topic>Cytokines</topic><topic>Gene Expression</topic><topic>Hepatitis</topic><topic>Hepatitis, Autoimmune - blood</topic><topic>Hepatitis, Autoimmune - genetics</topic><topic>Hepatitis, Autoimmune - immunology</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunoassay</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-gamma - genetics</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Interleukin-4 - genetics</topic><topic>Leukocytes</topic><topic>mRNA</topic><topic>Pediatrics</topic><topic>Polymerase Chain Reaction</topic><topic>Prognosis</topic><topic>RNA, Messenger - genetics</topic><topic>Th1</topic><topic>Th1 Cells - immunology</topic><topic>Th1 Cells - metabolism</topic><topic>Th2</topic><topic>Th2 Cells - immunology</topic><topic>Th2 Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawashima, Hisashi</creatorcontrib><creatorcontrib>Kato, Naoki</creatorcontrib><creatorcontrib>Ioi, Hiroaki</creatorcontrib><creatorcontrib>Nishimata, Shigeo</creatorcontrib><creatorcontrib>Watanabe, Chiako</creatorcontrib><creatorcontrib>Kashiwagi, Yasuyo</creatorcontrib><creatorcontrib>Takekuma, Kouji</creatorcontrib><creatorcontrib>Hoshika, Akinori</creatorcontrib><creatorcontrib>Szenborn, Leszek</creatorcontrib><creatorcontrib>Bergman, Kacprzak</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatrics international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawashima, Hisashi</au><au>Kato, Naoki</au><au>Ioi, Hiroaki</au><au>Nishimata, Shigeo</au><au>Watanabe, Chiako</au><au>Kashiwagi, Yasuyo</au><au>Takekuma, Kouji</au><au>Hoshika, Akinori</au><au>Szenborn, Leszek</au><au>Bergman, Kacprzak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>mRNA expression of T-helper 1, T-helper 2 cytokines in autoimmune hepatitis in childhood</atitle><jtitle>Pediatrics international</jtitle><addtitle>Pediatr Int</addtitle><date>2008-06</date><risdate>2008</risdate><volume>50</volume><issue>3</issue><spage>284</spage><epage>286</epage><pages>284-286</pages><issn>1328-8067</issn><eissn>1442-200X</eissn><abstract>Background: In the pathology of autoimmune hepatitis the immunity mechanism of T‐helper 1 (Th1) and Th2 cells was recently evaluated. The purpose of the present study was to measure the mRNA levels in peripheral mononuclear cells and serum cytokines obtained from children with autoimmune hepatitis for a better understanding of the mechanism.
Methods: Twenty‐five patients with autoimmune hepatitis and seven controls were enrolled. mRNA levels in peripheral mononuclear cells and serum cytokines were measured using real‐time polymerase chain reaction and immunoassay.
Results: Serum interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) were rarely detected. In contrast the IFN‐γ/β‐actin mRNA levels were high.
Conclusion: Autoimmune hepatitis is a Th1‐predominant state, therefore immune modulation therapies that target the control of Th1 cytokines should be used.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>18533937</pmid><doi>10.1111/j.1442-200X.2008.02584.x</doi><tpages>3</tpages></addata></record> |
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| ispartof | Pediatrics international, 2008-06, Vol.50 (3), p.284-286 |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Actins - biosynthesis Actins - genetics Adolescent Adult Autoimmune diseases autoimmune hepatitis Biomarkers - blood Child Child, Preschool childhood Cytokines Gene Expression Hepatitis Hepatitis, Autoimmune - blood Hepatitis, Autoimmune - genetics Hepatitis, Autoimmune - immunology Humans Immune system Immunoassay Interferon-gamma - biosynthesis Interferon-gamma - genetics Interleukin-4 - biosynthesis Interleukin-4 - genetics Leukocytes mRNA Pediatrics Polymerase Chain Reaction Prognosis RNA, Messenger - genetics Th1 Th1 Cells - immunology Th1 Cells - metabolism Th2 Th2 Cells - immunology Th2 Cells - metabolism |
| title | mRNA expression of T-helper 1, T-helper 2 cytokines in autoimmune hepatitis in childhood |
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