Evaluation of single-base substitution rate in DNA by affinity capillary electrophoresis

Capillary electrophoretic separation of 60 mer single-stranded DNA (ssDNA) and a single-base-substituted ssDNA was demonstrated using a size- and composition-controlled poly(ethylene glycol)-oligodeoxyribonucleotide block copolymer (PEG- b-ODN) as an affinity ligand. Under appropriate conditions, PE...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytica chimica acta 2008-06, Vol.619 (1), p.101-109
Hauptverfasser:	Kanayama, Naoki, Takarada, Tohru, Shibata, Hideaki, Kimura, Ayumi, Maeda, Mizuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity capillary electrophoresis Alleles Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Block copolymer Chemistry Chromatographic methods and physical methods associated with chromatography Codon Cytochromes b - genetics DNA DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics Electrophoresis, Capillary - methods Exact sciences and technology Fundamental and applied biological sciences. Psychology Hydrodynamic drag Ligands Magnesium - chemistry Molecular Sequence Data Mutation Oligonucleotide Probes - chemical synthesis Oligonucleotide Probes - chemistry Oligonucleotide Probes - genetics Other chromatographic methods Polyethylene Glycols - chemistry Polymerase Chain Reaction Polymorphism, Single Nucleotide Sensitivity and Specificity Single-base mutation Temperature
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!