DNA Sequence and Functional Analysis of Lactobacillus delbrueckii subsp. lactis Plasmids pN42 and pJBL2
The plasmids pN42 and pJBL2 were isolated from the Lactobacillus delbrueckii subsp. lactis strains NCC88 and JCL414. DNA sequence determination and bioinformatic analysis revealed a strikingly conserved genetic organization containing five major, highly conserved open reading frames (ORFs). Transfor...
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| Veröffentlicht in: | Plasmid 2002-03, Vol.47 (2), p.153-157 |
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| creator | Bourniquel, Aude A Casey, Michael G Mollet, Beat Pridmore, R.David |
| description | The plasmids pN42 and pJBL2 were isolated from the Lactobacillus delbrueckii subsp. lactis strains NCC88 and JCL414. DNA sequence determination and bioinformatic analysis revealed a strikingly conserved genetic organization containing five major, highly conserved open reading frames (ORFs). Transformation studies indicated that ORF2 (consisting of a primase fused to a replicative DNA helicase), ori, and ORF3 constitute the minimal requirements for replication of pN42 in the heterologous host Lactococcus lactis. The ORF1's are predicted to encode type I restriction-modification (R-M) system HsdS subunits with different specificities on either plasmid, suggesting that these plasmids may be involved in host defense by expanding their host R-M system repertoire. These plasmids constitute the basis for the construction of novel L. delbrueckii vectors. |
| doi_str_mv | 10.1006/plas.2001.1560 |
| format | Article |
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DNA sequence determination and bioinformatic analysis revealed a strikingly conserved genetic organization containing five major, highly conserved open reading frames (ORFs). Transformation studies indicated that ORF2 (consisting of a primase fused to a replicative DNA helicase), ori, and ORF3 constitute the minimal requirements for replication of pN42 in the heterologous host Lactococcus lactis. The ORF1's are predicted to encode type I restriction-modification (R-M) system HsdS subunits with different specificities on either plasmid, suggesting that these plasmids may be involved in host defense by expanding their host R-M system repertoire. These plasmids constitute the basis for the construction of novel L. delbrueckii vectors.</description><identifier>ISSN: 0147-619X</identifier><identifier>EISSN: 1095-9890</identifier><identifier>DOI: 10.1006/plas.2001.1560</identifier><identifier>PMID: 11982337</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; lactic acid bacteria ; Lactobacillus - genetics ; Lactobacillus delbrueckii ; Lactobacillus delbrueckii subsp. lactis ; Molecular Sequence Data ; Open Reading Frames ; ORF2 gene ; ORF3 gene ; plasmid ; plasmid pJBL2 ; plasmid pN42 ; Plasmids - genetics ; replication ; Replicon - genetics ; Sequence Analysis, DNA ; transformation ; type I R-M system</subject><ispartof>Plasmid, 2002-03, Vol.47 (2), p.153-157</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>Copyright 2002 Elsevier Science (USA).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-e2bde8dfa10cbaed77d6fc3836b5adca2a5d590d521d9fe7a7b534ed255fd4933</citedby><cites>FETCH-LOGICAL-c371t-e2bde8dfa10cbaed77d6fc3836b5adca2a5d590d521d9fe7a7b534ed255fd4933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/plas.2001.1560$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11982337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bourniquel, Aude A</creatorcontrib><creatorcontrib>Casey, Michael G</creatorcontrib><creatorcontrib>Mollet, Beat</creatorcontrib><creatorcontrib>Pridmore, R.David</creatorcontrib><title>DNA Sequence and Functional Analysis of Lactobacillus delbrueckii subsp. lactis Plasmids pN42 and pJBL2</title><title>Plasmid</title><addtitle>Plasmid</addtitle><description>The plasmids pN42 and pJBL2 were isolated from the Lactobacillus delbrueckii subsp. lactis strains NCC88 and JCL414. 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These plasmids constitute the basis for the construction of novel L. delbrueckii vectors.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>lactic acid bacteria</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus delbrueckii</subject><subject>Lactobacillus delbrueckii subsp. lactis</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>ORF2 gene</subject><subject>ORF3 gene</subject><subject>plasmid</subject><subject>plasmid pJBL2</subject><subject>plasmid pN42</subject><subject>Plasmids - genetics</subject><subject>replication</subject><subject>Replicon - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>transformation</subject><subject>type I R-M system</subject><issn>0147-619X</issn><issn>1095-9890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1P3DAQxa0KVBbaa4_IJ25JPXYcJ8fls0UrQKKVerMce1KZepMQJ0j89zjdlXqquMxcfu9p5j1CvgDLgbHy6xBMzDljkIMs2QeyAlbLrK5qdkBWDAqVlVD_OiLHMT6xJOBQfiRHAHXFhVAr8vvybk0f8XnGziI1naPXc2cn33cm0HUar9FH2rd0Y-zUN8b6EOZIHYZmnNH-8Z7GuYlDTkMCEvqQDtp6F-lwV_C_hsPt-YZ_IoetCRE_7_cJ-Xl99ePiW7a5v_l-sd5kViiYMuSNw8q1BphtDDqlXNlaUYmykcZZw410smZOcnB1i8qoRooCHZeydUUtxAk52_kOY5-eipPe-mgxBNNhP0etoCwKBfJdECoBrGRVAvMdaMc-xhFbPYx-a8ZXDUwvHeilA710oJcOkuB07zw3W3T_8H3oCah2AKYgXjyOOlq_5O_8iHbSrvf_834Dd1SWXQ</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Bourniquel, Aude A</creator><creator>Casey, Michael G</creator><creator>Mollet, Beat</creator><creator>Pridmore, R.David</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>DNA Sequence and Functional Analysis of Lactobacillus delbrueckii subsp. lactis Plasmids pN42 and pJBL2</title><author>Bourniquel, Aude A ; Casey, Michael G ; Mollet, Beat ; Pridmore, R.David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-e2bde8dfa10cbaed77d6fc3836b5adca2a5d590d521d9fe7a7b534ed255fd4933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>lactic acid bacteria</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus delbrueckii</topic><topic>Lactobacillus delbrueckii subsp. lactis</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>ORF2 gene</topic><topic>ORF3 gene</topic><topic>plasmid</topic><topic>plasmid pJBL2</topic><topic>plasmid pN42</topic><topic>Plasmids - genetics</topic><topic>replication</topic><topic>Replicon - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>transformation</topic><topic>type I R-M system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bourniquel, Aude A</creatorcontrib><creatorcontrib>Casey, Michael G</creatorcontrib><creatorcontrib>Mollet, Beat</creatorcontrib><creatorcontrib>Pridmore, R.David</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plasmid</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bourniquel, Aude A</au><au>Casey, Michael G</au><au>Mollet, Beat</au><au>Pridmore, R.David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Sequence and Functional Analysis of Lactobacillus delbrueckii subsp. lactis Plasmids pN42 and pJBL2</atitle><jtitle>Plasmid</jtitle><addtitle>Plasmid</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>47</volume><issue>2</issue><spage>153</spage><epage>157</epage><pages>153-157</pages><issn>0147-619X</issn><eissn>1095-9890</eissn><abstract>The plasmids pN42 and pJBL2 were isolated from the Lactobacillus delbrueckii subsp. lactis strains NCC88 and JCL414. DNA sequence determination and bioinformatic analysis revealed a strikingly conserved genetic organization containing five major, highly conserved open reading frames (ORFs). Transformation studies indicated that ORF2 (consisting of a primase fused to a replicative DNA helicase), ori, and ORF3 constitute the minimal requirements for replication of pN42 in the heterologous host Lactococcus lactis. The ORF1's are predicted to encode type I restriction-modification (R-M) system HsdS subunits with different specificities on either plasmid, suggesting that these plasmids may be involved in host defense by expanding their host R-M system repertoire. These plasmids constitute the basis for the construction of novel L. delbrueckii vectors.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11982337</pmid><doi>10.1006/plas.2001.1560</doi><tpages>5</tpages></addata></record> |
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| ispartof | Plasmid, 2002-03, Vol.47 (2), p.153-157 |
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| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism lactic acid bacteria Lactobacillus - genetics Lactobacillus delbrueckii Lactobacillus delbrueckii subsp. lactis Molecular Sequence Data Open Reading Frames ORF2 gene ORF3 gene plasmid plasmid pJBL2 plasmid pN42 Plasmids - genetics replication Replicon - genetics Sequence Analysis, DNA transformation type I R-M system |
| title | DNA Sequence and Functional Analysis of Lactobacillus delbrueckii subsp. lactis Plasmids pN42 and pJBL2 |
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